Pnck (NM_001199352) Mouse Untagged Clone

CAT#: MC227157

Pnck (untagged) - Mouse pregnancy upregulated non-ubiquitously expressed CaM kinase (Pnck), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pnck
• Synonyms: Bstk3; Camk1b; CaMKIbeta2; caMKlb1; CaMKlb2; Punc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227157 representing NM_001199352
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGCTCAAGAAACAGACGGAGGACATCAGCAGTGTCTATGAGATCCGGGAGAAGCTGGGCTCGG
  GTGCCTTCTCTGAGGTGATGCTGGCCCAGGAAAGGGGCTCTGCTCATCTTGTGGCCCTCAAGTGCATTCC
  CAAGAAAGCACTTCGGGGCAAGGAGGCCCTGGTGGAGAATGAGATCGCGGTACTTCGCAGAATCAGCCAT
  CCCAACATTGTGGCTCTGGAGGACGTCCATGAGAGTCCTTCTCATCTCTACTTGGCCATGGAGCTGGTAA
  CAGGTGGTGAACTGTTTGACCGCATCATGGAGCGGGGCTCCTACACAGAGAAGGACGCCAGCCACCTTGT
  AGGGCAGGTCCTTGGCGCTGTCTCCTACCTTCATAGCCTGGGCATCGTGCACCGGGACCTCAAGCCTGAA
  AACCTCCTCTATGCCACACCTTTTGAGGACTCCAAGATCATGGTCTCTGACTTTGGCCTGTCCAAAATAC
  AAGCTGGCAACATGCTAGGCACAGCCTGTGGGACCCCAGGATATGTGGCCCCAGAGCTCCTGGAGCAGAA
  ACCCTACGGGAAGGCCGTAGATGTGTGGGCCCTGGGTGTCATCTCCTACATCCTGCTGTGTGGGTACCCC
  CCCTTCTATGATGAGAGCGATCCTGAACTCTTCAGCCAGATTCTGAGGGCCAGCTATGAGTTTGACTCCC
  CCTTTTGGGATGACATCTCAGAATCAGCCAAAGACTTCATTCGCCACCTTCTGGAACGTGATCCCCAGAA
  GAGGTTCACCTGCCAGCAGGCCCTACAGCATCTTTGGATCTCTGGGGATGCAGCCTTCGATAGGGACATC
  CTGGGTTCTGTCAGTGAGCAGATCCAGAAGAATTTTGCCAGGACCCACTGGAAGCGTGCATTCAATGCCA
  CATCATTCCTACGTCACATCCGTAAGCTGGGACAAAGCCCAGAGGGTGAGGAGGCCTCCAGGCAGTGTAT
  GACCCGTCATAGCCACCCAGGCCTTGGGACTAGCCAGTCCCCCAAGTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199352
• Insert Size: 1032 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199352.1, NP_001186281.1
• RefSeq Size: 1506 bp
• RefSeq ORF: 1032 bp
• Locus ID: 93843
• UniProt ID: Q9QYK9
• Cytogenetics: X 37.38 cM
• Gene Summary: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) contains a different alternate exon in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein.