Abhd4 (NM_001205181) Mouse Untagged Clone

CAT#: MC226999

Abhd4 (untagged) - Mouse abhydrolase domain containing 4 (Abhd4), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Abhd4
• Synonyms: 1110035H23Rik; Abh4; AI429574
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226999 representing NM_001205181
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCAACTGAAGAATGTGGAAGCCAGGATCCTCCAGTGTCTCCAGAACAAGTTCCTGGCCCGTTATG
  TATCCCTCCCAAACCAGAACAAGATCTGGACGGTGACTGTGAGCCCAGAACAAAAGGATCGCACCCCTCT
  GGTGATGGTACATGGCTTTGGGGGCGGTGTGGGCCTCTGGATCCTCAACATGGATTCACTGAGTGCCCGC
  CGCACACTTCATACCTTTGATCTGCTTGGTTTTGGGCGAAGCTCAAGGCCAACATTCCCAAGGGACCCAG
  AAGGAGCTGAAGATGAGTTTGTGGCCTCAATAGAGACATGGCGGGAGACCATGGGAATCCCCACCATGAT
  CCTCCTGGGGCACAGTTTGGGAGGATTCCTGGCCACTTCTTACTCTATCAAGTACCCTGAAAGAGTTAAA
  CATCTTATCCTGGTGGATCCATGGGGCTTTCCCCTACGACCAACTGACCCTAGTGAGATCCGTGCACCTC
  CAACCTGGGTCAAGGCTGTGGCATCTGTCCTGGGACGTTCCAATCCACTGGCTGTTCTTCGAGTGGCTGG
  GCCTTGGGGGCCTGGGCTGGTGCAGAGATTCCGTCCAGACTTCAAGCGCAAGTTTGCAGACTTCTTTGAG
  GATGACACCATCTCGGAATACATCTACCACTGCAATGCACAGAATCCCAGTGGGGAAACGGCATTCAAAG
  CCATGATGGAGTCCTTTGGCTGGGCCCGGCGCCCCATGTTGGAGCGAATCCACTTAATTCGAAAAGATGT
  GCCCATCACCATGATCTATGGGGCCAACACCTGGATAGATACCAGCACAGGGAAGAAGGTGAAGATGCAA
  AGGCCGGATTCCTACGTCCGAGACATGGAGATCGAGGGCGCATCCCACCACGTCTATGCTGACCAGCCAC
  ACATCTTCAATGCTGTGGTAGAAGAGATCTGCAACTCAGTTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205181
• Insert Size: 957 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205181.1, NP_001192110.1
• RefSeq Size: 2661 bp
• RefSeq ORF: 957 bp
• Locus ID: 105501
• UniProt ID: Q8VD66
• Cytogenetics: 14 C2
• Gene Summary: Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis (PubMed:16818490, PubMed:25853435). Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains (PubMed:16818490, PubMed:25853435). Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine (PubMed:16818490).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.