Stx18 (NM_001289536) Mouse Untagged Clone

CAT#: MC226936

Stx18 (untagged) - Mouse syntaxin 18 (Stx18), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Stx18
• Synonyms: 1810035L21Rik; 4933425D03Rik; AU041562
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226936 representing NM_001289536
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGGACATCACACTGCTCTTCCGGGCTAGCGTCAAGACAGTGAAGACGCGGAATAAGGCTCTGG
  GAGTGGCCGTGGGTGGCGGGGCCGATGGCAGCCGGGACGAGCTGTTCCGCAGAAGCCCTCGGCCCAAGGG
  AGACTTCTCCAGCCGGGCCCGTGAAGTGATTTCTCACATTGGGAAGCTGAGAGATTTTCTCCTGGAGCAC
  AGGAAGGAGTATATTAATGCCTACAGCCACACCATGTCTGACTATGGGAGGATGACAGACACAGAGCGAG
  ACCAGATAGACCAGGATGCTCAGATATTCATAAGGACCTGTTCAGAAGCAATCCATCAACTCAGAACAGA
  AGGAGTGTGTAAGCTTTACTCTGAACAAAGAGCCATCCGAGTGAAGCGCGTGGTGGACAAGAAAAGACTA
  TCTAAGCTGGAACCTGAGCCACATACCAAGAGGAAAGACTCCACATCTGAGAAAGCCCCTCAGAACGCTT
  CCCAGGACTCTGAAGGAAAGCCTGCTGCTGAGGAGCTGCCAGAAAAGCCTTTGGCTGAATCACAGCCCGA
  ACTGGGAACCTGGGGCGATGGCAAGGGTGAAGATGAGCTGTCTCCAGAAGAGATACAGATGTTTGAACAA
  GAAAATCAGCGACTCATTGGTGAAATGAACAGCTTGTTTGACGAAGTGAGGCAAATCGAAGGGAAAGTCG
  TTGAAATTTCCAGACTCCAAGAGATATTCACCGAAAAGGTTTTGCAACAGGAAACTGAGATCGACAGCAT
  TCACCAGTTAGTCGTGGGGGCAACTGAAAATATTAAGGAAGGCAACGAAGACATCCGAGAGGCCATCAAA
  AACAATGCAGGTTTCCGAGTGTGGATCCTCTTCTTCCTCGTGATGTGTTCCTTTTCCTTGCTCTTCCTCG
  ACTGGTATGACAGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289536
• Insert Size: 927 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289536.1, NP_001276465.1
• RefSeq Size: 2441 bp
• RefSeq ORF: 927 bp
• Locus ID: 71116
• UniProt ID: Q8VDS8
• Cytogenetics: 5 B3
• Gene Summary: Syntaxin that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.