Eqtn (NM_001290623) Mouse Untagged Clone

CAT#: MC226873

Eqtn (untagged) - Mouse equatorin, sperm acrosome associated (Eqtn), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eqtn
• Synonyms: 1700028B15Rik; 4930579C15Rik; Afaf; C81058; Eqtl; Eqts
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226873 representing NM_001290623
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTTATACTGTTGATCATTTTATCTGGGGTTTTCCTCCCAGACATTATCAGTCTACAGCCCATTG
  TGGGACAGGAGCCTGGTGTGACACTTTCAGATGAAGAACAGTATTATGCAGATGAAGAAAATAATACAGA
  TGGGAATTCTGTTGCTTTACATAAATTGGAAGAGAATGAAATGGACACTCCTGCCAATGAGAAAACTGGC
  AATTATTATAAAGACATAAAACAATATGTGTTCACCACACCAAACATAAAAGGTTCTGAAGTATCTGTGA
  CTGCCACAACTAACCTGGAATTTGCTGTGAAGAAGAACTATAAAGCCAGCAAACCAACTGCCAGTGGAGA
  AGAAGAAAAACCTAGTGAATCCTCTCGTAAAACCTCAACCCCCAATATACCTGCATTTTGGACAATATTA
  TCTAAAGCTGTAAATGAAACAGCAGTGAGCATGGATGATAAAGATCAATTTTTTCAACCAATTCCAGCCT
  CAGATTTGAATGCTACCAATGAAGACAAACTGTCAGAGCTCGAGGAAATCAAGCTGAAGTTAATGCTGGG
  GATCTCGCTGATGACACTGGTTCTGCTAATCCCCCTCTTGATATTCTGCTTTGCCACACTGTACAAACTG
  AGACACCTACGTGACAAAAGTTATGAGAGTCAATACTCCATCAACCCAGAGCTGGCAACTCTATCTTACT
  TCCATCCAACAGAAGGCTCCAATCAAACAGTCTTAACCGACGAGTCAAGCTTCCTCCCTCCTGAGGAGAC
  AAGATTCCTCCTTCCTGAGGAGCCGGGTAAGGAGCTCATCGTTGAGAGAGGCCCTATGCAGGCAATGAAT
  GAGATTGATGCCCAGCTGTTGTTGAACAAGGAAGGATCACCGAGTAATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290623
• Insert Size: 891 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290623.1, NP_001277552.1
• RefSeq Size: 1164 bp
• RefSeq ORF: 891 bp
• Locus ID: 67753
• UniProt ID: Q9D9V2
• Cytogenetics: 4 C5
• Gene Summary: Acrosomal membrane-anchored protein involved in the process of fertilization and in acrosome biogenesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses alternate in-frame splice sites in the 3' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter, compared to isoform 1.