Rnf144a (NM_001081977) Mouse Untagged Clone

CAT#: MC226842

Rnf144a (untagged) - Mouse ring finger protein 144A (Rnf144a), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rnf144a
• Synonyms: Rnf144; Ubce7ip4; UIP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226842 representing NM_001081977
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACAGCAAGGTACCGGCCCACCTGGGACCTGGCCCTCGACCCACTGGTGTCCTGCAAGCTGTGCC
  TCGGGGAATACCCAGCGGAGCAGATGACAACCATCGCCCAATGCCAGTGCATCTTCTGTACCCTGTGCCT
  GAAACAGTATGTTGAGCTCTTGATCAAAGAAGGGCTAGAAACTGCAATTAGCTGCCCGGATGCTGCCTGT
  CCCAAACAGGGCCACCTTCAGGAGAATGAGATTGAGTGTATGGTTGCCGCAGAAATAATGCAGAGATATA
  AAAAGCTACAATTTGAAAGAGAGGTGCTCTTTGACCCTTGTCGGACGTGGTGCCCAGCATCCACCTGCCA
  GGCCGTGTGCCAACTCCAGGACATAGGGCTGCAGACCCCGCAGCTGGTACAGTGCAAAGCCTGTGACATG
  GAATTCTGCTCTGCTTGCAAAGCCCGCTGGCACCCTGGCCAAGGCTGCCCAGAGACCATGCCCATCACAT
  TCCTTCCCGGAGAGACCAGCTCTGCTTTCAAGATGGAAGAGGGCGACGCACCCATCAAACGCTGCCCCAA
  GTGCAGGGTGTACATCGAGCGGGATGAGGGCTGTGCACAGATGATGTGTAAGAACTGCAAGCATGCCTTC
  TGCTGGTACTGCCTGGAGTCTCTGGATGATGATTTCCTTCTGATACATTATGACAAGGGGCCCTGCCGCA
  ACAAGCTGGGCCACTCCCGAGCATCAGTGATCTGGCATCGGACACAGGTGGTGGGAATTTTTGCTGGATT
  TGGGCTCCTGCTCTTAGTGGCCTCCCCTTTCCTGCTCCTGGCCACACCCTTCGTGCTTTGCTGCAAATGC
  AAGTGCAGTAAAGGTGACGACGACCCACTGCCCACCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081977
• Insert Size: 879 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081977.2, NP_001075446.1
• RefSeq Size: 5189 bp
• RefSeq ORF: 879 bp
• Locus ID: 108089
• UniProt ID: Q925F3
• Cytogenetics: 12 A2
• Gene Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.