Taf9 (NM_001015889) Mouse Untagged Clone

CAT#: MC226664

Taf9 (untagged) - Mouse TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor (Taf9), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Taf9
• Synonyms: 2310012M09Rik; AA673500; AU045423; Taf2g; TAFII31; TAFII32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226664 representing NM_001015889
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCTGGCAAGATGGCGTCTCCCAAGAGCATGCCGAAAGATGCACAGATGATGGCACAAATCCTGA
  AGGATATGGGGATTACAGAATATGAGCCAAGAGTTATAAATCAGATGCTGGAGTTTGCCTTCCGATATGT
  GACAACAATTCTAGATGATGCTAAAATTTATTCCAGCCATGCTAAGAAAGCTACTGTTGATGCAGATGAC
  GTACGACTGGCAATCCAGTGCCGTGCTGACCAGTCTTTCACTTCTCCACCCCCGAGAGATTTCTTATTAG
  ATATTGCACGACAAAGAAATCAAACCCCTTTGCCACTGATCAAGCCATATTCAGGTCCTAGATTGCCACC
  TGATAGGTATTGCTTAACTGCCCCAAACTATAGGCTTAAGTCTTTACAGAAAAAGGCACCTGCTCCTGCA
  GGAAGAATAACAGTTCCAAGGTTAAGTGTTGGTTCAGTTTCTAGTAGACCAAGTACTCCCACCCTAGGTA
  CACCGACTCCACAAACCATGTCTGTTTCAACTAAAGTAGGCACCCCAATGTCCCTCACAGGACAGAGGTT
  TACAGTACAGATGCCTGCTTCTCAGTCCCCTGCTGTAAAAGCTTCTATACCTGCAACATCAACAGTTCAG
  AATGTTCTTATTAATCCATCGTTAATTGGGTCCAAAAACATTCTTATTACTACTAATATGGTATCACAGA
  ATACAGCCGAGTCAGCAAATGCACTGAAACGGAAACGTGAAGACGATGATGATGACGATGATGACGATGA
  TGATGATGACTATGATAATATGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001015889
• Insert Size: 795 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001015889.2, NP_001015889.1
• RefSeq Size: 1248 bp
• RefSeq ORF: 795 bp
• Locus ID: 108143
• UniProt ID: Q8VI33
• Cytogenetics: 13 D1
• Gene Summary: Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) represents the longer transcript. Variants 1 and 3 encode the same protein.