Cyb561a3 (NM_001282064) Mouse Untagged Clone

CAT#: MC226518

Cyb561a3 (untagged) - Mouse cytochrome b561 family, member A3 (Cyb561a3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cyb561a3
• Synonyms: 2310004G04Rik; Cybasc3; Lcytb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226518 representing NM_001282064
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCAGGATGGTTTTACCTGTCCTGCATGGTGCTGGGATCGCTGGGATCGATGTGCATCCTCTTCA
  CTGCCTACTGGATGCAGTACTGGCGCGGTGGCTTTGCCTGGGATGGCACGGTGCTCATGTTTAACTGGCA
  CCCAGTGCTCATGGTTGCCGGCATGGTGGTGCTCTATGGAGCTGCCTCACTGGTGTACCGCCTGCCTTCA
  TCGTGGGTGGGGCCCAGGCTGCCCTGGAAAGTTCTCCATGCAGCACTGCACCTGCTGGCCTTCACCTGCA
  CTGTGGTGGGGCTGATTGCCGTCTTTCGGTTTCACAACCACTCGAGAATCGCACACCTCTACTCCCTGCA
  CAGCTGGCTGGGTATCACCACTGTAGTCCTCTTCGCCTGCCAGTGGTTCCTGGGCTTTGCTGTCTTCCTC
  CTGCCCTGGGCATCCCAGTGGCTGCGAAGCCTCCTGAAACCTCTGCATGTATTCTTTGGAGCCTGCATCC
  TTTCCCTGTCCATCACATCTGTTATTTCCGGCATCAATGAGAAGCTTTTCTTTGTTTTGAAAAATGCCAC
  CAAGCCCTACTCCAGCCTGCCTGGTGAGGCTGTCTTTGCCAACAGCACAGGGCTCTTGGTGGTGGCTTTT
  GGCTTGCTGGTTCTCTATGTTCTTCTGGCTTCATCATGGAAGCGTCCAGATCCAGGAGCTCTGACTGATA
  GACAGCCCCTGTTGCATGACAGGGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282064
• Insert Size: 729 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282064.1, NP_001268993.1
• RefSeq Size: 3167 bp
• RefSeq ORF: 729 bp
• Locus ID: 225912
• UniProt ID: Q6P1H1
• Cytogenetics: 19 A
• Gene Summary: Ferric-chelate reductase that reduces Fe(3+) to Fe(2+) before its transport from the endosome to the cytoplasm. Probably uses ascorbate as electron donor.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 4 encode the same isoform (1).