Il15ra (NM_001271500) Mouse Untagged Clone

CAT#: MC226232

Il15ra (untagged) - Mouse interleukin 15 receptor, alpha chain (Il15ra), transcript variant 6

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Il15ra
• Synonyms: AA690181; IL-15RA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226232 representing NM_001271500
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGCCGCAGCTCCGGGGCTATGGAGTCCAGGCCATTCCTGTGTTGCTGCTGCTGCTGTTGCTAC
  TGTTGCTCCCGCTGAGGGTGACGCCGGGCACCACGTGTCCACCTCCCGTATCTATTGAGCATGCTGACAT
  CCGGGTCAAGAATTACAGTGTGAACTCCAGGGAGAGGTATGTCTGTAACTCTGGCTTTAAGCGGAAAGCT
  GGAACATCCACCCTGATTGAGTGTGTGATCAACAAGAACACAAATGTTGCCCACTGGACAACTCCCAGCC
  TCAAGTGCATCAGAGACCCCTCCCTAGCTCACTACAGTCCAGTGCCAACAGTAGTGACACCAAAGGTGAC
  CTCACAGCCAGAGAGCCCCTCCCCCTCTGCAAAAGAGATTTCTCCCCACAGTTCCAAAATGACGAAAGTG
  GCCATCTCTACATCGGTCCTCTTGGTTGGTGCAGGGGTTGTGATGGCTTTCCTGGCCTGGTACATCAAAT
  CAAGGCAGCCTTCTCAGCCGTGCCGTGTTGAGGTGGAAACCATGGAAACAGTACCAATGACTGTGAGGGC
  CAGCAGCAAGGAGGATGAAGACACAGGAGCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271500
• Insert Size: 594 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271500.1, NP_001258429.1
• RefSeq Size: 1466 bp
• RefSeq ORF: 594 bp
• Locus ID: 16169
• UniProt ID: Q60819
• Cytogenetics: 2 8.97 cM
• Gene Summary: High-affinity receptor for interleukin-15 (PubMed:17947230). Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells (PubMed:17947230). In neutrophils, binds and activates kinase SYK in response to IL15 stimulation (By similarity). In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 5 (also known as isoform 1B) which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.