Rpain (NM_001252415) Mouse Untagged Clone

CAT#: MC226073

Rpain (untagged) - Mouse RPA interacting protein (Rpain), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rpain
• Synonyms: 2400006N03Rik; Rip
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC226073 representing NM_001252415
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGTCCTCGGGGTCTCCGCACCGCTTGTTGTACAAGCAGGTGGGCTCGCCCCACTGGAAAGAAA
  CTTTCAGGCAGGGATGTCTGGAGAGAATGAGAAACAGCAGGCACAGGCTCCTGAACAAATATCGCCAGGC
  TGCAGGTAGCACGCCGGGGACAGCCTCAGACAGACTTCTTGTGCAAGAAGTAATGGAGGAAGAGTGGGCT
  TCTTTGCAGTCTGTGGAGAATTGTCCGGAGGCCTTGCTTCAGTTGGAATTGCCACTGGACCTAGCTGTGC
  TGCAGGACATCGAGCAGGAGCTGTGTAATGAAGAAAAGTCCATCATAAGCGAGTATGAAGAGGACTTAGA
  GTTTGATGAAAGTTGTCTCAGGAGAATGTTGGCTGAGTGGGAAGCAAACTCCCTCATCTGTCCTGTGTGT
  ATAAAGTACAACCTGAGAATAATGAACAGTGTGGTCACGTGTCCATGTGGCCTGCACATCCCTGTTCACT
  CACCGGTGGAACAGAAGAGAAGCCCAGTCTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252415
• Insert Size: 525 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252415.1, NP_001239344.1
• RefSeq Size: 1119 bp
• RefSeq ORF: 525 bp
• Locus ID: 69723
• UniProt ID: Q9CWY9
• Cytogenetics: 11 B4
• Gene Summary: Mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. Sumoylation mediates the localization of RPA complex into the PML body of the nucleus, thereby participating in RPA function in DNA metabolism (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.