Glrx2 (NM_001038594) Mouse Untagged Clone

CAT#: MC225757

Glrx2 (untagged) - Mouse glutaredoxin 2 (thioltransferase) (Glrx2), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Glrx2
• Synonyms: 1700010P22Rik; AI645710; Grx2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225757 representing NM_001038594
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAACAGCACATCGTCGTTTTGGGGGAAGTCTACAACTACTCCTGTGAACCAGATCCAAGAAACAA
  TTTCTAACAATTGTGTGGTGATCTTCTCAAAAACATCCTGCTCTTACTGTTCCATGGCCAAGAAGATTTT
  CCATGACATGAATGTCAACTACAAGGCTGTGGAGTTGGATATGCTGGAATATGGCAACCAGTTTCAAGAT
  GCGCTTCACAAGATGACTGGGGAAAGAACCGTTCCCAGGATATTTGTCAATGGACGATTTATTGGAGGCG
  CAGCGGACACTCACAGGCTTCACAAAGAAGGGAAATTGCTGCCTCTGGTTCATCAGTGTTATTTAAAAAA
  AAAACAAGAGGAAAGACATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001038594
• Insert Size: 372 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001038594.1, NP_001033683.1
• RefSeq Size: 3445 bp
• RefSeq ORF: 372 bp
• Locus ID: 69367
• UniProt ID: Q923X4
• Cytogenetics: 1 62.53 cM
• Gene Summary: Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.[UniProtKB/Swiss-Prot Function]