Syne1 (NM_001079686) Mouse Untagged Clone

CAT#: MC225445

Syne1 (untagged) - Mouse synaptic nuclear envelope 1 (Syne1), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Syne1
• Synonyms: 8B; A330049M09Rik; BE692247; C130039F11Rik; CPG2; mKIAA1756; myne-1; Myne1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225445 representing NM_001079686
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCCTCAGGAGCTGGCGCAGAAAATCAAGGGCTACCAGGAGCAGATCGACTCTTTGAATTCCAAAT
  GCAAGATGCTGACGATGAAAGCCAAGCACGCAACTATGCTGCTGACTGTGACCGAGGTCGAGGGACTGGC
  TGAAGGGACCGAGGATCTGGACCGCGAGCTCCACCCTACGCCTTCAGCCCACCCCTCTGTGGTCATGATG
  ACTGCAGGTCGCTGTCATACTCTGCTGTCACCTGTCACTGAAGAGTCTGGGGAAGAGGGAACCAACAGTG
  AGATTTCCTCTCCACCTGCCTGTCGCTCCCCATCACCTGTGGCTAACACGGAGGCGGCTGTTAACCAGGA
  CATTGCGTACTACCAAGCTCTGTCAGCCGAGGGGTTGCAGACAGATGCTGCAAGAATTCCCCCCAGTGCT
  GCTGTATCCCAAGAGTTGTATGAGCCAGGACTGGAGCCTTCAGCGACTGCCAAGCTGGGTGACTTACAAC
  GTTCCTGGGAAACCTTAAAAAACGTGATCAGTGAGAAACAGCGGACTCTCTACGAAGTTTTGGAACGCCA
  GCAGAAGTACCAAGACTCTCTCCAATCCATCTCGACTAAAATGGAGGCCATGGAGATGAAGCTAGGTGAG
  AGCCTCGAGCCCAGCCGGAGTCCAGAGAGCCAGATGGCTGAGCACCAGGCCCTGATGGACGAGGTGCAGA
  TGCTACAAGACGAGATCAATGGGCTCCAGGTGTCTCTTGCTGAGGAGCTGGTGGCCGAGTCTCAGGAGTC
  GGACCCCGCGGAGCAGCTAGCTCTGCAGTCCACGCTCACTGTCCTGGCTGAGAGAATGTCCACCATCAGG
  ATGAAAGCAGCTGGCAAGCGGCAGCTTCTGGAGGAGAAGCTAAGTGATCAGCTGGAGGAGCAGAGACAGG
  AGCAGGCTCTGCAGAGGTACCGCTGCGAGGCAGATGAGCTGGACCACTGGCTGCTGAACACTAAGGCTAC
  CCTGGATGTTGCACTGGGTACATCCCAGGAGCCCATGGACATGGATGCCCAGCTGGTGGACTGCCAGAAC
  ATGCTGGTGGAAATCGAACAGAAAGTGGTGGCCCTGTCGCAGCTGTCCGTGCACAATGAGAACCTGCTGT
  TGGAGGGCAAGGCTCACACTAAAGAGGAGGCCGAGCAGCTAGCGGTGAAGCTGAGGCTACTCAAAGGGAG
  CCTTGGAGAGCTGCAGAGGGCCCTGCATGACAGACAGCTCGACATGCAGGGAGTCACACAAGAGAAAGAG
  GAGAACGATGTTGACTTCACAGACACCCAGAGCCCCGGCGTGCAGGAATGGCTGGCACAAGCCCGCACCA
  CAAGGACCCACCAGCGGCAGAGCAGCCTCCAGCAGCAAAAAGAGTTCGAACAGGAGCTGGCCGAGCAGAA
  GAGCCTCCTCCGCTCAGTAGCTAGCCGTGGAGAGGAGATTCTAACCCAACATTCCACCGCAGAAGGTTCA
  GGTGGCCTTGGTGAAAAACCTGATGTGTTATCCCAGGAGTTGGGGATTGCTGAAGACCAGATGAGAGTGA
  AGTGGGAAAGCCTACACCAAGAGTTTAGTGCCAAGCAGAAGCTACTACAGAATATTCTGGAACAGGAGCA
  AGAACAAGTGCTTTACAGTAGTCCGAACAGACTCCTGTCAGGAGTACTTCCATTCAGAGGGGAAGCACAG
  ACTCAGGATAAGACTTCAGTGACGTCCTTGCTGGATGGGCTGAGCCAGGCCTTCGGGGAGGCGTCATCAC
  AGAGTGGAGGGACAGACAGACAGAGCATTCACTTGGAGCAGAAGCTATATGACGGAGTCTCAGCCACTTC
  CACCTGGTTGAATGACGTCGAGGAGCGCTTATTTGTAGCCACGGCACCTTTGCCAGAAGAAACAGAAGCT
  TGTCTCTTCAACCAGGAGGCTCTTGCCAAGGACATTAAGGAAATGTCTGAAGAAATGGATAAGAACAAGA
  ACTTGTTTTCCCAAGCATTCCCAGAGGACAGTGACAACCGGGATGTCATTGAAGATACTCTGGGTTGTCT
  TTTGGGCAGATTGTCCCTGCTGGATTCAGTAGTGGATCAGCGGTGTCATCAGATGAAGGAAAGACTCCAG
  CAAATCCTCCGTTTCCAGAATGACCTGAAGGTGTTGTTTACATCGTTAGCTGACAGCAAATATATCATTC
  TGCAGAAGCTGGCAAATGTGTTTGAACAGCCCATTGTGGAACAGATGCAGGCCATCCAACAGGCTGAAGA
  GGGACTCAGGGACCTCGAGGGTGGAATCTCTGAGTTGAAACGGTGGGCTGACAAGTTGCAAGTGGAACAG
  TCTGCTGTGCAGGAACTCTCCAAGCTCCAGGACATGTATGACGAGCTATTGATGACTGTCAGCTCCCGGA
  GGAGCAGCCTCCACCAGAACCTTGCTCTCAAGAGTCAGTATGATAAGGCTCTGCAAGATCTGGTGGACCT
  GCTGGACACTGGACAGGAGAAGATGACAGGGGACCAAAAGATCATTGTATGCTCCAAAGAAGAAATCCAG
  CAGCTGCTTGGGAAGCACAAGGAATACTTCCAGGGCCTGGAATCGCACATGATTTTGACTGAAATACTCT
  TCAGGAAGATCGTTGGCTTTGCAGCTGTGAAGGAAACTCAGTTCCATACGGACTGCATGGCTCAGGCCTC
  TGCTGTGTTGAAGCAGGCTCACAAGAGAGGGGTGGAGCTTGAGTACATCCTAGAGATGTGGTCCCATCTG
  GATGAGAATCGCCAGGAGCTCAGCAGACAGCTGGAGGTGATAGAAAACAGCATCCCAAGTGTGGGCCTGG
  TAGAAGAGAGTGAGGACAGACTTGTGGAAAGGACAAATCTCTACCAGCATTTAAAATCCAGCCTCAATGA
  ATACCAGCCCAAACTGTACCAAGCACTGGATGATGGGAAGCGACTTCTGATGTCTGTCAGCTGTTCAGAG
  CTTGAAAGTCAGCTAAATCAGCTGGGAGAGCACTGGCTCAGTAATACGAACAAAGTATCCAAGGAGCTGC
  ACAGACTGGAGACCATACTGAAACACTGGACCAGATATCAAAGTGAAGCTGCAGCTCTCAACCACTGGCT
  ACAGTGCGCTAAGGACAGGCTGGCATTTTGGACTCAGCAGTCTGTGACGGTTCCACAAGAACTGGAAATG
  GTCCGGGATCATCTCAGTGCTTTCCTGGAGTTCTCTAAAGAAGTAGATGCCAAGTCTGCCCTCAAGTCGT
  CAGTCACAAGCACAGGAAATCAGCTGCTTCGGTTAAAAAAAGTGGACACAGCGGCCCTGCGTGCTGAGCT
  GTCCCGCATGGACAGCCAGTGGACTGACCTACTGACCGGCATCCCTGTTGTACAGGAGAAGCTCCATCAG
  CTCCAGATGGACAAGCTGCCTTCCCGCCATGCCATTTCTGAGGTAATGAGTTGGATCTCACTCATGGAAA
  GTGTTATCCTGAAGGATGAAGAGGACATTAGAAATGCCATCGGTTACAAGGCGATCCACGAATACCTTCA
  GAAATACAAGGGTTTCAAGATAGACCTTAACTGCAAGCAGCTAACGGCGGACTTTGTGAACCAGTCAGTG
  CTCCAAATCAGCAGTCAGGACGTGGAGAGCAAGCGCAGTGACAAGACGGATTTTGCTGAGCAGCTTGGGG
  CAATGAATAAAAGTTGGCAGCTCTTGCAAGGTCGAGTTGGAGAGAAGATCCAGATGTTGGAAGGCTTGTT
  GGAGTCATGGTCAGAGTATGAAAACAGTGTCCAGTCTCTGAAAGCCTGGTTTGCAAACCAGGAGAGGAAG
  CTGAAAGAGCAGCATCTACTTGGCGATCGGAATTCTGTTGAGAATGCTCTGAAGGACTGTCAGGAACTGG
  AAGATTTGATTAAAGCAAAAGAAAAGGAGGTAGAGAAAATTGAGCAGAATGGCCTCGCATTGATTCAGAA
  CAAGAGGGAGGAGGTCTCTGGCAGTGTCATGAGCACACTGCAAGAACTCAGGCAGACCTGGATCAGTTTA
  GACCGCACGGTTGAACAATTAAAGATCCAGCTGACCTCAGCCCTTGGCCAGTGGAGCAATCACAAAGCAG
  CCTGTGACGAGATCAACGGTCACCTCATGGAGGCCAGATACTCCCTGTCCCGATTCCGTCTGTTGACAGG
  TTCCTCCGAAGCTGTACAAGTTCAGGTCGACAATCTTCAGAATCTTCATGATGAACTAGAAAAACAGGAA
  GGAGGCTTACAGAAATTTGGCTCTATCACCAACCAACTATTGAAGGAGTGTCACCCACCTGTGGCAGAAA
  CCCTCTCCAGTACTCTGCAAGAAGTCAATATGAGATGGAATAACTTACTGGAAGAGATCGCTGAACAGCT
  GCACTCCAGCAAGGCCCTCCTTCAGCTCTGGCAGAGATACAAGGACTACTCAAAGCAGTGTGCCTCTGCC
  ATCCAGAGACAAGAGGAACAGACCAGTGTACTGTTGAAGGCAGCCACTAACAAGGATATCGCTGATGATG
  AAGTCACCAAGTGGATTCAGGATTGCAACGACCTTCTCAAGGGACTGGAGACAGTCAAGGACTCGCTTTT
  CATCCTCCGTGAACTAGGAGAGCAGCTTGGACAGCAGGTGGATGTTTCCGCAGCAGCAGCCATTCAGTGT
  GAGCAGCTCTGTTTCAGCCAGCGCCTGGGGGCCCTGGAGCAGGCTCTTTGCAAGCAGCAAGCAGTATTGC
  AGGCTGGAGTTGTTGACTATGAAACATTTGCCAAGAGCTTAGAAGCCTTAGAGGTCTGGATGGTGGAAGC
  CGAGGGCATCCTCCAAGGACAGGATCCCACCCATTCCTCTGACCTCTCTACTATCCAGGAAAGGATGGAG
  GAACTGAAGGGACAGATGCTAAAGTTCAGCAGCTTGGCTCCTGATTTAGACCGTCTCAATGAGTTGGGGT
  ACCGGTTACCCTTAAATGACAAGGAGATCAAGAGGATGCAGAACCTGAACCGCCATTGGTCTCTGACCTC
  TTCTCAGACCACAGAAAGATTCAGCAAGTTGCAGTCATTCTTGCTTCAGCACCAGACTTTCTTGGAAAAG
  TGTGAGACATGGATGGAATTTCTGGTTCAGACAGAGCACAAGTTGGCAGTGGAGATCTCAGGCAATTACC
  AGCATCTTTTGGAGCAGCAAAGAGCACATGAGCTTTTTCAAGCCGAGATGTTCAGCCGCCAGCAGATCCT
  CCACTCCATCATTGTTGATGGGCAGAATCTTCTAGAACAAGGTCAAGTTGATGATAGGGAAGAGTTCAGC
  CTGAAGTTGACGCTCCTCAGTAACCAGTGGCAGGGAGTGATTCGAAGGGCCCAACAGAGAAGGGGGATCA
  TTGACAGCCAGATTCGCCAGTGGCAACGCTATAGGGAAATGGCAGAGAAGCTCCGTAAATGGTTGGCTGA
  GGTGTCTCATCTGCCCCTGAGTGGTCTCGGGAACATCCCAGTACCACTGCAACAAGTGAGGATGCTCTTT
  GATGAAGTTCAGTTCAAAGAGAAGGTGTTCCTGCGCCAGCAAGGGAGCTACATCCTGACAGTGGAGGCTG
  GCAAGCAGCTCCTGCTCTCTGCCGACAGTGGCGCCGAGGCCGCCCTGCAGGCTGAGCTCACAGACATCCA
  AGAGAAGTGGAAGGCAGCCAGCATGCATCTGGAGGAGCAGAAGAAAAAGCTGGCTTTTCTGTTGAAAGAC
  TGGGAAAAATGTGAGAGAGGGATAGCCAATTCTCTGGAGAAGCTCCGAATGTTCAAAAAGAGGTTATCTC
  AGCCTCTACCTGACCACCATGAGGAACTTCATGCAGAGCAGATGCGGTGTAAGGAACTGGAAAACGCAGT
  TGGGAGGTGGACAGATGACCTGACAGAGTTGATGCTGGTGAGGGATGCCCTGGCTGTCTACCTCAGTGCT
  GAGGACATCTCCATGCTGAAGGAACGCGTGGAGCTTCTGCAAAGACAATGGGAAGAGCTTTGTCACCAGG
  TCTCCCTACGACGACAGCAAGTAAGCGAGCGCCTGAATGAGTGGGCTGTCTTCAGTGAAAAGAACAAGGA
  GCTGTGTGAGTGGCTGACCCAGATGGAGAGCAAAGTCTCTCAGAACGGAGACATTCTCATCGAAGAGATG
  ATAGAAAAACTCAAGAAGGATTATCAAGAGGAAATTGCTGTTGCCCAAGAGAACAAAATCCAGCTTCAGG
  AAATGGGAGAACGGCTTGCTAAAGCCAGCCACGAGAGCAAAGCCTCTGAGATTCAGTACAAGCTCAGCAG
  AGTGAAGGACAGGTGGCAGCATCTCCTGGACCTCATGGCAGCTAGGGTGAAGAAGCTAAAGGAGACGCTG
  GTTGCTGTGCAGCAGCTGGACAAGAACATGGGCAGCCTGAGGACTTGGCTGGCCCACATGGAGTCAGAGT
  TGGCCAAGCCCATAGTCTATGATTCTTGTAACTCTGAAGAAATACAGAGGAAACTGAATGAGCAGCAGGA
  GCTCCAGAGAGACATCGAGAAACACAGCACAGGTGTCGCCTCCGTCCTCAACCTGTGTGAGGTTCTGCTG
  CATGACTGCGACGCCTGTGCCACCGACGCAGAGTGTGACTCCATCCAGCAGGCCACTCGCAACCTGGACC
  GGCGGTGGAGGAACATCTGCGCCATGTCCATGGAGAGGAGACTCAAAATTGAAGAGACGTGGCGACTGTG
  GCAGAAGTTTCTGGATGACTATTCTCGCTTTGAAGACTGGCTGGAGGTTTCAGAAAGGACGGCTGCTTTC
  CCCAGTTCTTCCGGGGTGCTCTATACAGTTGCCAAGGAGGAGCTGAAGAAGTTTGAGGCTTTCCAGCGGC
  AGGTCCATGAGAGCCTGACCCAACTAGAGCTTATCAACAAACAGTACCGGCGCCTGGCCAGAGAGAATCG
  CACAGACTCCGCCTGCAGCCTCAGGCAGATGGTCCATGGGGGCAACCAGAGATGGGACGACCTACAGAAG
  CGGGTCACCTCCATCTTGCGCAGGCTCAAGCACTTCATTAGCCAGCGTGAGGAGTTCGAGACTGCCCGGG
  ACAGCATTCTTGTCTGGCTCACAGAGATGGATCTGCAGCTCACCAATATTGAACACTTTTCGGAATGTGA
  CGTTCAAGCTAAAATAAAGCAACTCAAGGCTTTCCAACAGGAGATCTCACTGAACCACAATAAAATCGAG
  CAGATCATTGCCCAGGGGGAGCAGCTGATAGAGAAGAGCGAGCCACTGGATGCCGCTGTCATCGAGGAGG
  AACTGGACGAGCTCAGACGCTACTGCCAGGAGGTCTTTGGACGTGTGGAAAGATATCACAAGAAACTGAT
  CCGCCTGCCTCTGCCAGATGACCATGACCTCTCAGACCGAGAACTAGATCTCGAAGACTCCACGGCTCTT
  TCAGACCTCCGCTGGCAGGACCCGTCTGCAGATGGCATGCCCTCCCCACAGCCTTCTTCCAACCCCTCCC
  TCTCCCTTCCTCAGCCCCTCCGGAGTGAGCGGTCAGGGCGAGATACGCCGGCCAGCGTGGACTCCATCCC
  CCTGGAATGGGACCACGATTACGACCTCAGTCGAGACCTAGAGTCAGCATCCCGAACTCTGCCCTCAGAA
  GATGAAGAAGGCGAGGAAGACAAGGAGTTCTACCTCAGGGGAGCTGTTGGCTTGTCAGGGGATCCCAGCT
  CACTGGAGTCACAGATGAGGCAACTGGACAAAGCCCTGGATGACAGCCGCTTCCAGATACAGCAAACCGC
  AAATATCCTCAGAAGCAAAACCCCCACAGGGCCAGACCTGGACACCAGCTACAAGGGCTACATGAAGCTG
  CTGGGCGAGTGCAGTGGCAGCATAGATTCCGTGAGGAGACTGGAGCACAAGCTGGCGGAGGAAGAGAGCT
  TTCCTGGCTTCGTTAACCTCAACAGCACAGAGACTCAGACAGCTGGTGTAATTGACCGCTGGGAGCTCCT
  GCAGGCCCAGGCAATGAGCAAGGAGTTGAGGATGAAGCAGAACCTTCAGAAGTGGCAGCAGTTTAATTCT
  GACCTCAACAACATCTGGGCCTGGCTAGGAGAGACAGAGGAGGAGCTGGACAGGCTCCAGCACCTGGCGC
  TCAGCACTGACATCCACACCATCGAGTCTCACATCAAAAAGCTCAAGGAGCTCCAGAAAGCCGTGGACCA
  CCGCAAAGCCATCATCCTCTCCATCAACCTCTGCAGCTCTGAGTTCACCCAGGCTGACAGCAAAGAGAGT
  CATGACCTGCAGGATCGCTTATCCCAGATGAATGGGAGATGGGATCGAGTGTGCTCTCTGCTGGAGGACT
  GGAGAGGCTTGCTGCAGGATGCACTGATGCAGTGCCAGGAGTTCCATGAAATGAGCCATGCCTTGCTTCT
  CATGCTAGAAAACATTGACAGAAGGAAAAATGAAATTGTCCCAATTGATTCAACCCTTGACCCAGAGACA
  CTTCAGGACCATCACAAGCAACTCATGCAAATAAAGCAGGAGTTGCTGAAGTCCCAACTCAGAGTGGCCT
  CACTACAAGACATGTCTCGCCAACTCTTGGTGAATGCTGAAGGTTCAGATTGTCTAGAAGCCAAAGAAAA
  GGTCCATGTGATTGGAAACCGGCTCAAACTTCTCCTGAAGGAGGTCAGCCATCATATCAAGGATCTTGAG
  AAGTTACTAGACATGTCAAGCAGTCAGCAGGATTTGTCATCATGGTCTTCTGCAGATGAGCTGGACACCT
  CAGGATCTGTGAGTCCCACATCCGGAAGAAGTACCCCAAACAGACAGAAATCGCCACGAGGCAAATGTAG
  TCTCTCACAGCCTGGACCCTCTGTCAGCAGCCCAAAGAGCAGGTCCACAAGAGATGGCTCCGATTCCTCC
  CGTTCTGACCCCAGGCCAGAACGGGTGGGTCGAGCCTTCCTGTTCCGGATCCTCCGGGCAGCTCTTCCCT
  TTCAGCTGCTCCTGCTGCTGCTTATTGGACTCACCTGCCTTGTACCCATGTCAGAGAAAGACTACAGCTG
  TGCCCTCTCCAACAACTTTGCCCGATCCTTCCATCCGATGCTCAGATATACCAACGGTCCTCCTCCACTC
  TGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001079686
• Insert Size: 9033 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079686.1, NP_001073154.1
• RefSeq Size: 10876 bp
• RefSeq ORF: 9033 bp
• Locus ID: 64009
• UniProt ID: Q6ZWR6
• Cytogenetics: 10 A1
• Gene Summary: Multi-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment. During interkinetic nuclear migration (INM) at G2 phase and nuclear migration in neural progenitors its LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes functions to pull the nucleus toward the centrosome; SYNE1 and SYNE2 seem to act redundantly in cerebellum, midbrain, brain stem, and other brain regions except cerebral cortex and hippocampus. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenenis; a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region and lacks a large portion of the 5' coding region found in variant 4. These differences result in the use of an alternate start codon, compared to variant 4. The encoded isoform (3) is shorter and has distinct N-terminus compared to isoform 4.