Abca12 (NM_175210) Mouse Untagged Clone

CAT#: MC225368

Abca12 (untagged) - Mouse ATP-binding cassette, sub-family A (ABC1), member 12 (Abca12), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Abca12
• Synonyms: 4832428G11Rik; 4833417A11Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225368 representing NM_175210
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCCAGTTTCATCAGCTTCGGATCCTGGTGTGGAAAAATTGGCTAGGTGTCAAAAGGCAGCCGC
  TTTGGACACTTGTCTTGATCTTATGGCCAGTCATTATTTTCATAATTCTGGCTATTACCCGGACAAAATT
  CCCGCCAACAGCAAAACCAACTTGTTACCTCGCACCTCGAAACCTTCCCAGTGCTGGATTCTTCCCATTC
  CTACAAACCCTGCTCTGTGACACAGACTCTAAATGTAAAGACACGCCCTATGGACCACGAGATCTGCTCC
  GCAGGAAGGGGATTGATGGACCGCTATTTAAAGAAAGCGAAATTCTGAAAAAGCCGTCCAACCCGAAGAG
  GGACAGCAATTTATCACTCCGGAGCACCCAAGTTCCAGAAAGAAGTCACACATCATTGGCCACAGTACCT
  CCAAGACCAAGCTATGATTTGGAAGGAACAGGCACAGAAAATTTCAATGGTAGCCAATTGCTTACACGCA
  TCCTTGGTTTGGAAAAGCTGCTGAAGCAAAATTCAACTCCAGAAGACATACGAAGAGAGCTGTGTGAGAG
  CTACCCTGGGTACACTGCGGACTATGCCTTCTCTTGGGTCACCCTAGGAAAAAACGTTTTTAACAAATTT
  TGCCTTTCGAACATGACACTTTTAGAGTCTTCACTCCAGGAACTAAAATATCAAGTCTCTCAGATGTCAA
  GTGACCCCGACAATCAGAAGAGGGTGTTCCGGGGACTTGTCCAGGTGCTGTCCTTTTTCTCTCAAGTGCA
  GCAGCAAAGAGAAGTGTGGCAACTTCTGTCGAGCTTACCAGATGTGTTTCAGAATGGCACCTCATTGAGC
  AGTCTATTTGGTGTTCTTCAAAAGGCAAACAGGGTGCTGCTGGTAGTGCAGAAGGTTTACCCACGTGTTC
  AAACTGATGAAGGATTCAGCACCCTCCAGAAGTCTGTTAAACATCTGCTGAACACTCTGGACTCCCCAAT
  GCAAGGTGACAATTCAACGCACGCATGGAGTGATGATGATGAGCAGACACTGTCCCCGAGCAGTCTGGCT
  GCACAGCTCCTGATCCTGGAAAACTTTGAAGATGCCATCTTGAACATATCATCAAATAGTCCATATTCTC
  CTTATTTGGCGTGTGTAAGGAACATGACTGACAACTTGGCTAAGGGATCACCAGACAATCTAAAACTTCT
  GCAGTCCACAATTCACTTCAGAAAATCTTTCCTTCAAAATGGCTCCTCCGAGGACAGCTTCCCTCCATTT
  CTTGAGATCCTGAAATCCAAGCTGTCTCAACTTCGAAATTTGACGGAACTTCTCTGCGAATCAGAGACTT
  TCAGTTCGATCAAGAAGTCTTGCCAGTTCTCTAACATGAGCTTCGAGAGGCTGTGTGAAGACCATGCCTT
  TCACGTGCAACTGATTGAAGCGGCAGAGCTGGGCACTGACCTCACCACTGGCTTACTGTACCATGACAAT
  ATAATATCTGCAAAGCTGAGGGGTTTGCTCACTGGAGATCCCAGCAAAATTAATCTCAATGTGGACTGGC
  TCCTGGAACAGGCATTGCAAATGAATTACTTGGAGAATATCACACGGCTCATACCAACCGTAGAGGCCAT
  GCTGCATGTCAACACCAGTGCAGACGCTTCTGAGAAGCCAGGTCAATTAAGAGAAATGTTTAAAAACATA
  GATTTGCTGAAAGAAGATCTGAGGGCGATAGGGATGTCCAACACAAGTATCGACAAGCTGTTGGCCATTC
  CCATTCCAGACAATAGAGCCGAGATAATTTCTCGTGTATTCTGGTTGCACTCATGTGATACCAACGTCAC
  CAACCCCAAACTGGAAGATGCCATGAAGGAGTTCTGCAAGCTGCCTCTTCCAGAGAGATCACATCAGTCC
  TACCTCATCGGACTCACCCTTCTGCACTACTTAGACATTTACAATTTCACATACAAGGTGTTTTTCCCAA
  GGAAGGATCAAAAGCCAATGGAAAGGATGATGGAGCTTTTCATAAAGCTGAGAGAGATTCTCAATCAGTT
  GGCTTCCGGCACACATCCACTGCTGGACAAAATGAGATCCCTGAGACAAATGCACCTGCCCAGGAGTGTT
  CCATTAACACAGGCCATGTACAGAAACACTCGAATGAACTCGCCAGCAGGATCGTTCAGTACTATCTCCC
  AGGCTCTGTGCTCCCAGGGGATCACCACCGAGTACTTAACTGCTATGCTGCCTTCTTCCCAGAAGCCAAA
  AGGCAACCACACCAAGGATTTTCTCACTTATAAATTAACTAAAGAAGAAATTGCTTCAAAATACGGGATT
  CCTTTAAATGCTACACCATTTTGCTTCTCCCTTTACAAAGACATCATTAACATGCCTGCTGGGCCTGTGA
  TTTGGGCCTTCTTGAAACCCATGTTGTTGGGAAAGATTTTATATTCACCATATAACCCAACTACAAAGGC
  CATTATGGAAAAGTCCAATGTAACTCTAAGACAGCTGGCGGAATTAAGAGAAAAATCTCAGGAGTGGATG
  GATAAGTCACCAATTTTCATGAATTCCTTCCACCTGCTAAACCAGACGATCCCAATGCTCCAGAATACTC
  TACGGAACCCCTTTGTGCAAGTTTTTGTGAAGTTCTCCGTGGGACTCGATGCTGTCGAACTCCTGAAGCA
  GATAGACGACCTTGATGTTCTCAGGCTGAAATTAGTGAACAACATTGACATCATTGATCAGCTCAACACA
  CTGTCTTCCCTGACTGTAAATATTTCCTCCTGTGTTCTTTACGACCGTATTCAGGCGTCAGACACTGTAG
  AGGAGATGGAGACGGTGGCCGAACAACTCTACAAGAGCAACGAGCTCTTTGGAAGTGTCATTTTTAAGCT
  TCCATCTAACGGAAGTTTGCACAGAGGGTTTGACCCTGAAAAAGTGTCTCTTCCTCCTATCGTAAGATAT
  ACCATCCGCATGAGTCTGAAGACGGCACAGACCACAAGGAGCATAAGAACAAAGATCTGGGCCCCAGGGC
  CACACAACTCTCCATCCCATAACCAGATCTATGGCCGGGCTTTCATTTATCTGCAGGATAGCATTGAGAG
  AGCAATTATTGAGTTACAAACAGGAAGGAACTCACAGGAAGTCGCCGTCCAGGTTCAAGCGGTTCCTTAT
  CCCTGCTTCATGAAGGACAATTTCCTCACCAGTGTCTCCTATTCCCTCCCGATTGTGCTTATGGTCGCCT
  GGGTTGTATTTATAGCTGCCTTTGTTAAAAAGCTAGTCTACGAGAAGGACCTTCGGCTCCATGAGTACAT
  GAAGATGATGGGTGTCAACTCCTGCAGCCATTTCTTTGCCTGGCTTATAGAGAGTATTGGGTTCTTGCTG
  GTCACCATTGCAATCCTCATCGTTATCCTCAAGTTTGGCAATATTCTTCCCAAGACAAACGGCTTCATTT
  TGTTCCTGTACTTCTCAGACTACAGCTTCTCAGTGATTGCCATGAGCTATCTCATCAGTGTCTTCTTCAA
  CAACACCAACATTGCAGCTCTGATTGGAAGCCTCATCTATGTCATCGCCTTTTTCCCATTTATTGTTCTG
  GTTACAGTTGAGGATGAGCTGAGCTACGTTATTAAAGTATTCATGAGCCTGCTGTCCCCGACAGCATTCA
  GCTATGCAAGCCAATATATTGCACGGTATGAAGAACAAGGCGTTGGTCTTCAGTGGGAAAATATGTACAA
  ATCCCCTGTTCAGGATGACACCACCTCATTTGGCTGGCTGTGCTGTCTAATCCTAGCAGACTCCTTCATT
  TATTTCTTTATTGCTTGGTACGTCAGGAATGTTTTCCCAGGGACATATGGAATGGCAGCTCCATGGTATT
  TTCCCATCCTTCCTTCCTATTGGAAAGAGCGCTTTGGATGCGCAGAGGTGAAGCATGAGAAAAGCAATGG
  CTTAATGTTTACTAATATCATGATGCAGAACACCAACCCTTCTGCCAGTAAGACAAGTCCTGACTGTGCA
  TTTCCCTCCAACATTGAACCTGAACCTAAAGATCTCCAAGTTGGAGTTGCCCTTCATGGGGTCACAAAGA
  TCTATGGCTCAAAAACAGCTGTTGAGAACCTCAATCTGAACTTCTACGAAGGGCACATTACATCACTGTT
  GGGGCCAAACGGAGCTGGCAAAACCACAACAATCTCCATGCTGACTGGGCTGTTTGGTGCCACAGCGGGT
  ACCATTTTTGTATACGGAAAAGATATCAAGACAGACCTGAATACTGTTCGGAAAAACATGGGGGTCTGCA
  TGCAACATGACGTCTTGTTCAGTTACCTAACCACCAAGGAACACCTTCTCCTGTATGGCTCCATCAAAGT
  TCCTCACTGGACCAAAACACAGCTCCATGAGGAGGTTAAAAGGACTTTAAAAGACACTGGACTGTATAGT
  CATCGCCATAAGAGAGTTGGAACGTTGTCGGGAGGAATGAAGAGGAAGTTGTCCATATCCATAGCTCTCA
  TCGGTGGCTCCAGGGTAGTGATTTTGGATGAACCATCCACGGGCGTTGACCCATGTTCTCGCCGAAGCAT
  ATGGGATGTCATATCCAAGAACAAAACTGCAAGAACAATCATTCTGTCCACACACCACTTGGATGAGGCC
  GAAGTGCTGAGTGACCGCATCGCCTTCCTGGAACAGGGTGGGCTCCGGTGCTGTGGATCTCCATTTTACC
  TCAAGGAAGCTTTTGGGGATGGTTACCACCTCACACTCACCAAGAAGAAGAGTCCAAACCTAGACACAAA
  TGCAATCTGTGACACAGTGGCAGTGACAGCAATGATCCAGTCGCACCTTCCTGAAGCCTACCTCAAAGAG
  GATATTGGGGGAGAGCTTGTATATGTGCTCCCTCCATTCAGCACGAAAGTCTCAGGAGCTTACCTGTCCC
  TCCTGAGAGCACTGGACAAGGGCATGGGTAAACTCAACATCGGCTGCTATGGCATTTCAGACACCACTGT
  TGAAGAGGTCTTCCTGAACTTGACAAAAGATTCGCAAAAAAGTAGTAATATGAGTCTGGAGCACTTAACA
  CAGAGGAAAGTTGGGAACCCCAGTGCCAACGGGACCTCCACTCCTGACGACCTGTCTGTGAGCAGCAGCA
  ACTTCACCGACAGAGACGACAAAGTCCTGACAAGAAGCGAGAAGCTGGAGGGCTTTGGACTCTTACTGAA
  GAAGATAATGGCTATTCTCATCAAGAGGTTCCACCACACCCGCCGGAACTGGAAAGGTCTCATTGCTCAG
  GTCATTCTCCCCATCGTCTTTGTAGCCACGGCCATGGGTCTCGGCACACTGAGAGATTCCAGCAACAGTT
  ACCCAGAGATCATGATCTCTCCGTCCATTTACGGCACCTCCGAACAGACAGCCTTCTACGCGAATTTTGA
  TCCAAGCACCAGTGGTCTGGTCTCAGCTCTCTGGAATTTCCCTGGCATTGACAATGTGTGTTTGAATACT
  AGTGACTTGCAGTGTTTAAAAAAAGATGATCTGGGAAAGTGGAACACAAGTGGAGAAGCTATCGACAACT
  TTGGTGTTTGCTCCTGTTCCGACAATGTCCAGGAATGCCCCAAATTCAACTATCATCCACCTCATAGAAG
  AACTTACTCTTCCCAAGTTATTTATAACCTCACTGGAAAACACATGGAAAATTATCTTATAACAACTGCA
  AATCATTTTGTGCAGAAAAGATATGGAGGTTGGAGTTTTGGGATGAAATTGACTAACGACCTTCGTTTTG
  ATGTGACAGCTGTCCCTGACAATAGAACTCTTGCTAAGGTGTGGTACGATCCGGAAGGCTACCATTCCCT
  CCCAGCTTACCTCAACAGCCTCAACAACTTCCTCCTGAGGGTTAACATGTCAGAGTACGATGCTGCCCGA
  CACGGGATCATCATGTATAGCCACCCTTATCCAGGGGTACAAGACCAGGAACAAGCCACAATCAGCAGTT
  TAATCGATATTTTAGTGGCGCTGTCCATCCTGATGGGCTACTCTGTCACCACTGCCAGCTTTGTCACCTA
  CATTGTCAGGGAACATCAGACCAAAGCCAAGCAGTTGCAGCACATTTCTGGCATCGGTGTGACGTGCTAC
  TGGGTGACAAATTTCATCTATGACATGGTCTTCTATTTGGTTCCTGTAGCATTTTCAATTGGTGTCATCG
  CAATTTTCAAGCTTCCTGCCTTCTACAGTGGAAACAATCTAGGCGCTGTATCCCTCCTACTTTTGCTGTT
  TGGCTATGCAACATTTTCTTGGATGTACCTGCTGGCTGGCCTCTTCCACGAAACAGGAATGGCCTTCATC
  ACTTATGTGTGTGTCAACCTGTTCTTTGGTATCAATTCCATCGTTTCCCTGTCGGTGGTATACTTCCTTT
  CCAAGGAAAAGCCTAATGACCCGACGCTAGAACTTATTTCTGAAACTCTCAAGCGGATTTTCCTGATCTT
  CCCCCAATTCTGTTTTGGCTACGGTTTGATTGAACTTTCTCAGCAACAGGCAGTCTTGGATTTCTTAAAA
  GCCTACGGTGTGGAATATCCAAGCGAGACCTTTGAGATGGATAAGCTGGGGGCAATGTTTGTGGCCTTGG
  TTTCTCAGGGCACCATGTTTTTCTTGTTGCGACTCCTCATCAATGAGTGGCTGATAAAGAAGCTCAGGCT
  CTTCTTCAGGAAATTTACATCTTCGCCTATCATGGAGACAGTAGATGAAGATGAAGATGTGCGGGCTGAG
  AGATTTCGAGTTGAGAGTGGTGCAGCTGAGTTTGACCTGGTCCAGCTCCATCGTCTCACAAAGACCTACC
  AGCTGATCCACAAGAAGATTATAGCTGTCAACAACATCAGCCTGGGGATACCTGCTGGAGAGTGTTTTGG
  CCTCCTTGGTGTGAACGGAGCAGGGAAAACCACTATATTTAAGATGCTGACTGGAGACATAATTCCTTCC
  AGTGGAAACATTCTGATCAGAAACAAGAGCGGATCCCTGGGACATGTGGATTCTCACAGCTCTTTGGTTG
  GCTACTGCCCTCAGGAGGACGCTTTAGATGACCTAGTAACTGTGGAAGAGCACTTGTATTTCTATGCCAG
  AGTGCATGGGATTCCAGAGAAGGACATCAAAGACACTGTTCATAAACTCCTTAGGAGACTTCACTTGATG
  GCCTACAAGGACAGATCTACCTCCATGTGCAGTTACGGCACAAAAAGGAAGCTGTCCACTGCCCTGGCCT
  TGATAGGGAAACCGTCCATCCTCCTGCTGGATGAGCCAAGCTCTGGGATGGATCCCAAGTCAAAACGACA
  CCTCTGGAGGATCATTTCAGAAGAGGTGCAGAACAAATGCTCTGTCATCCTCACATCTCACAGCATGGAA
  GAATGTGAAGCTCTTTGTACCCGGCTGGCCATTATGGTGAACGGAAGGTTCCAGTGCATTGGGTCTCTGC
  AACACATAAAAAGCAGGTTTGGACGTGGATTTACTGTCAAGGTTCACTTGAAAAATAATAAAGTGAGCAT
  GGAAACCCTCACGAAGTTTATGCAGCTACACTTTCCAAAAACATACTTAAAAGATCAGCACCTGAGCATG
  CTGGAGTACCACGTGCCAGTCACAGCAGGAGGGGTCGCAAACATCTTTGACCTTCTGGAAACCAACAAGA
  CTGCTTTAAATATCACAAATTTCCTCGTGAGCCAGACCACTTTGGAGGAGGTTTTCATCAACTTTGCCAA
  AGACCAAAAATCCTACGAAAATGTTGATACCAGTAGCCAAGGTTCCACCATAAGTGTAGACTCACAGGAA
  GATCAACTAGATTCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175210
• Insert Size: 7788 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175210.3, NP_780419.2
• RefSeq Size: 8323 bp
• RefSeq ORF: 7788 bp
• Locus ID: 74591
• UniProt ID: E9Q876
• Cytogenetics: 1 C3
• Gene Summary: Transports lipids such as glucosylceramides from the outer to the inner leaflet of lamellar granules (LGs) membrane, whereby the lipids are finally transported to the keratinocyte periphery via the trans-Golgi network and LGs and released to the apical surface of the granular keratinocytes to form lipid lamellae in the stratum corneum of the epidermis, which is essential for skin barrier function (PubMed:18957418, PubMed:27551807, PubMed:24293640, PubMed:20489143, PubMed:18802465). In the mean time, participates in the transport of the lamellar granules-associated proteolytic enzymes, in turns regulates desquamation and keratinocyte differentiation (PubMed:27551807, PubMed:20489143). Furthermore, is essential for the regulation of cellular cholesterol homeostasis by regulating ABCA1-dependent cholesterol efflux from macrophages through interaction with NR1H2 and ABCA1 (PubMed:18802465, PubMed:23931754). Plays pleiotropic roles in regulating glucose stimulated insulin secretion from beta cells, regulating the morphology and fusion of insulin granules, lipid raft abundance and the actin cytoskeleton (PubMed:32072744). Also involved in lung surfactant biogenesis (PubMed:18632686).[UniProtKB/Swiss-Prot Function]