Ep300 (NM_177821) Mouse Untagged Clone

CAT#: MC225306

Ep300 (untagged) - Mouse E1A binding protein p300 (Ep300), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ep300
• Synonyms: A430090G16; A730011L11; KAT3B; p300; p300 HAT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225306 representing NM_177821
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGAATGTGGTGGAACCCGGGCCGCCTTCAGCCAAGCGGCCTAAACTCTCATCTCCGGCCCTCT
  CGGCGTCCGCCAGCGATGGCACAGATTTTGGTTCACTGTTTGACCTGGAACATGACTTACCAGATGAATT
  AATCAACTCTACAGAATTGGGACTAACCAATGGTGGCGATATCAGTCAGCTTCAGACAAGTCTTGGCATA
  GTACAAGATGCAGCCTCGAAACATAAACAGCTGTCAGAACTGCTGAGGTCTGGTAGCTCCCCAAACCTCA
  ACATGGGAGTCGGTGGCCCAGGCCAAGCGATGGCCAGCCAGGCCCAACAGAACAGCCCTGGATTAAGTTT
  GATAAATAGCATGGTCAAAAGCCCAATGGCACAGACAGGCTTGACTTCTCCAAACATGGGGATTGGCAGT
  AGTGGACCAAATCAGGGTCCTACTCAGTCCCCAGCAGGTATGATGAACAGTCCAGTGAACCAGCCTGCCA
  TGGGAATGAACACAGGGATGAATGCTGGCATGAATCCTGGAATGTTGGCTGCAGGCAATGGACAAGGGAT
  AATGCCCAATCAAGTCATGAACGGTTCCATTGGAGCAGGCCGGGGACGGCCAAACATGCAGTACCCAAAT
  GCAGGCATGGGCAATGCTGGCAGTTTATTGACTGAGCCACTACAGCAGGGCTCTCCTCAGATGGGAGGAC
  AGCCAGGATTGAGAGGCCCCCAACCACTTAAGATGGGAATGATGAACAATCCCAGTCCTTATGGTTCACC
  ATACACTCAGAATTCTGGACAGCAGATTGGAGCAAGTGGCCTTGGTCTCCAAATTCAGACAAAGACTGTT
  CTACCAAATAACTTATCTCCATTTGCAATGGACAAAAAGGCAGTTCCTGGTGGGGGAATGCCCAGTATGG
  GCCAGCAGCCTACCCCATCGGTCCAGCAGCCAGGCCTGGTGACTCCAGTTGCCGCAGGAATGGGTTCTGG
  AGCACACACAGCTGATCCAGAGAAGCGCAAGCTCATCCAGCAGCAGCTTGTTCTCCTTTTACATGCTCAC
  AAGTGCCAGCGCCGGGAGCAAGCTAATGGGGAAGTGAGGCAGTGCAACCTTCCTCACTGTCGTACCATGA
  AAAATGTCCTAAACCATATGACACATTGCCAGTCAGGCAAATCCTGCCAAGTGGCACATTGTGCATCTTC
  TCGACAAATCATTTCACACTGGAAAAATTGCACAAGGCATGATTGTCCTGTGTGTCTTCCTCTCAAAAAT
  GCTGGGGATAAGCGAAATCAACAGTCAATTTTGACTGGAGCACCAGTTGGGCTTGGAAACCCTAGCTCTC
  TAGGAGTGGGGCAGCAGTCCACTCCTAGCCTAAGCACTGTTAGTCAGATTGACCCCAGCTCTATAGAGCG
  AGCTTACGCTGCTCTTGGACTACCCTATCAAGTAAACCAGATTCCACCACAACCCCAGGTACAAGCAAAG
  AATCAACAAAGCCAGCCATCTGGACAGTCTCCCCAGGGCATGCGGTCTGTGAACAACATGAGTGCTAGTC
  CTATGGGTGTAAATGGAGGTGTTGGGGTTCAGACGCCAAATCTTCTTTCTGACTCCATGTTGCATTCAAC
  TATAAATTCTCAAAACCCAATGATGAGTGAAAATGCTGGTGTGGCCTCCCTGGGTCCTTTGCCAACAGCA
  GCTCAGCCATCTAGTACTGGAATTCGAAAACAGTGGCATGAAGATATTACTCAGGATCTTCGAAACCACC
  TTGTTCATAAACTTGTTCAAGCCATATTTCCCACTCCGGATCCTGCTGCTTTAAAAGACCGACGGATGGA
  AAATCTTGTTGCATATGCTCGTAAAGTGGAAGGGGACATGTATGAATCTGCAAACAATAGAGCGGAATAC
  TATCACCTCCTAGCCGAGAAGATCTATAAGATCCAGAAGGAACTAGAAGAGAAACGAAGGACTAGACTAC
  AGAAACAGAACATGCTACCGAATGCTCCTGGCATGGGTCCAGTTCCTATGAATACTGGGTCGAACATGGG
  GCAGCAACCAACAGGAATGACTACCAATGGTCCTGTACCTGACCCGTCTATGATCCGTGGCAGTGTGCCG
  AACCACATGATGCCTCGGATGACTCCACAGCCTGGTTTGAATCAATTTGGGCAGATGAATATGCCACAGC
  CCCCTATTGGACCCCGGCAACCCTCTCCTCTTCAGCACCATGGACAATTGGCTCAGTCTGGGTCACTCAA
  TCCGCCTATGGGCTATGGACCTCGCATGCAGCAGGCTTCCGGCCAGAACCAGTTCCTCTCCCAGACTCAG
  TTCACATCCCAAGGAATGAATGTAACAAACATGCCTTTGGCTCCATCCAGCGGTCAAGCACCAGTGTCTC
  AAGCACAAATGTCCAGTTCTTCTTGCCCAGTGAACTCTCCTATAATGCCTCCAGGGTCTCAAGGGAGCCA
  CATTCACTGTCCTACTCTGCCTCAACAAGCTCATCAGAATTCACCTTCTCCTGTTCCTAGCCGCACCCCT
  ACTCCTCATCATACCCCCCCAAGCATAGGGAATCAACCACCACCAGCAACAGCAATTCCAACACCTGTTC
  CTACACCTCCTGCCATACCACCTGGACCACAGCCCCCATCTCTGCATCCCTCATCTAGACAAACACCTAC
  ACCACCAACACATCTGCCTCCCCAAGTGCAGCCTTCACTTCCTGCTGCTCCTTCTGCTGACCAGTCCCAG
  CAACAGCCTCGGTCACAGCAGAGCACAGCAGTTTCTGTTCCTACCCCAACAGCACCATTGCTGCCTCCTC
  AGCCTTCCACTCCGCTTTCTCAGCCAGCTGTGAGCATTGAAGGCCAGGTCTCAAACCCTCCATCTACTAG
  TAGCACCGAAGTGAATTCTCAGACCATTCCTGAGAAGCAGCCTTCACAGGAAGTGAAAATGGAGTCTAAA
  ATGGAGGTGGATAAGCCAGAACCAGCAGATGCTCAACCTGAGGATACAAAGGAGGCTAAAGGTGAGGATG
  TTAAAGTAGAACCTACAGAAATGGAGGAGAGAGGCCCTGAGTTAAAAACTGATGGGAAAGAGGAGGAAGA
  ACAGCCAAGCACCTCTGCTACCCAGTCCTCCCCAGCTCCTGGACAGTCAAAGAAGAAGATTTTCAAACCA
  GAGGAATTACGACAGGCTCTGATGCCAACATTGGAGGCACTTTACCGGCAGGACCCAGAATCTCTTCCCT
  TTCGTCAGCCTGTGGACCCGCAGCTTCTAGGAATCCCTGATTACTTTGATATTGTGAAGAGCCCCATGGA
  TCTTTCTACAATCAAGAGGAAGTTAGATACTGGACAATACCAAGAGCCCTGGCAATATATAGATGATATT
  TGGCTTATGTTCAACAATGCCTGGTTATATAACCGTAAAACTTCAAGGGTATACAAATATTGCTCTAAAC
  TTTCTGAAGTATTTGAACAAGAAATTGACCCTGTCATGCAAAGCCTTGGATACTGTTGTGGCAGAAAGTT
  GGAGTTTTCTCCACAGACTCTTTGTTGCTATGGGAAACAGTTATGCACAATCCCCCGTGATGCTACTTAC
  TACAGTTACCAGAACAGGTATCATTTCTGTGAGAAGTGTTTCAATGAAATCCAAGGGGAGAGCGTTTCTT
  TGGGTGATGACCCTTCCCAACCTCAAACTACAATAAATAAAGAACAGTTTTCCAAGAGAAAAAATGACAC
  GTTGGATCCTGAACTGTTTGTTGAGTGCACAGAGTGTGGAAGAAAGATGCACCAGATCTGTGTCCTTCAC
  CACGAGATCATCTGGCCATCTGGGTTTGTCTGTGATGGCTGTTTAAAGAAAACTGCACGAACTAGGAAAG
  AAAATAAGCTTTCTGCTAAAAGATTGCCATCTACCAGACTTGGGACCTTTCTGGAGAATCGAGTGAATGA
  CTTTCTGAGGCGACAAAATCACCCTGAATCAGGAGAGGTCACTGTTCGGGTTGTTCATGCTTCTGACAAA
  ACTGTGGAAGTGAAACCAGGCATGAAAGCAAGGTTTGTAGATAGTGGAGAGATGGCAGAATCTTTTCCAT
  ACCGAACAAAGGCCCTGTTTGCCTTTGAAGAAATTGATGGTGTTGACTTGTGTTTCTTCGGCATGCATGT
  TCAAGAATATGGCTCTGACTGCCCCCCTCCCAACCAGAGGAGGGTATACATATCTTACCTCGATAGTGTT
  CATTTCTTCCGTCCTAAATGCTTGCGGACTGCAGTCTATCATGAAATTCTAATTGGATATTTGGAATATG
  TCAAGAAATTAGGATACACAACAGGGCACATCTGGGCCTGTCCACCCAGTGAAGGGGATGACTATATCTT
  CCATTGCCATCCACCTGATCAGAAGATACCAAAGCCCAAGCGCCTGCAAGAATGGTACAAAAAGATGCTT
  GACAAGGCTGTATCAGAACGTATTGTCCATGACTACAAGGATATTTTAAAACAAGCTACTGAAGATCGAT
  TAACAAGTGCAAAGGAACTACCCTACTTTGAAGGTGATTTCTGGCCCAATGTTCTAGAAGAAAGCATCAA
  GGAGCTTGAACAGGAAGAGGAAGAGAGGAAACGGGAAGAGAACACCAGCAACGAGAGTACTGATGTAACA
  AAAGGAGACAGCAAAAATGCTAAGAAGAAGAATAACAAGAAAACCAGCAAAAACAAGAGCAGCCTGAGTA
  GGGGCAATAAGAAGAAGCCTGGTATGCCCAATGTGTCTAATGACCTTTCTCAGAAACTGTATGCCACCAT
  GGAAAAGCATAAAGAGGTTTTCTTTGTGATCCGCCTCATCGCTTGTCCTGCTCCCAATTCCCTGCCTCCC
  ATTGTTGATCCTGACCCTCTCATCCCCTGTGACCTGATGGACGGTAGAGATGCATTTCTCACCCTTGCAA
  GGGACAAGCACCTGGAGTTCTCTTCACTTCGAAGAGCCCAGTGGTCTACCATGTGCATGCTGGTGGAGCT
  CCATACACAGAGTCAGGATCGCTTTGTCTACACCTGCAACGAGTGCAAGCACCACGTGGAGACACGCTGG
  CATTGCACTGTGTGTGAGGATTATGACCTGTGCATCACTTGTTATAACACTAAAAATCATGACCACAAAA
  TGGAGAAACTAGGCCTTGGCTTAGATGATGAGAGCAATAACCAGCAAGCTGCAGCTACACAGAGCCCAGG
  AGACTCCCGCCGCCTGAGCATCCAACGCTGCATCCAGTCTCTGGTGCATGCCTGTCAGTGCCGCAACGCC
  AACTGCTCCCTGCCTTCCTGCCAGAAGATGAAGAGGGTTGTGCAGCACACCAAAGGCTGCAAACGGAAAA
  CCAATGGTGGGTGCCCCATTTGCAAACAGCTTATTGCCCTCTGCTGCTATCATGCCAAGCACTGCCAGGA
  GAACAAGTGCCCGGTGCCATTTTGCCTCAACATCAAGCAAAAGCTCCGGCAGCAACAGCTGCAGCACCGG
  CTCCAACAGGCTCAGATGCTCCGTAGGAGGATGGCCAGCATGCAACGAACTGGTGTGGCGGGGCAGCAGC
  AGGGCCTGCCGTCCCCAACTCCTGCTACACCAACTACCCCTACTGGCCAACAGCCAGCCACCCCACAGAC
  ACCCCAGCCCCAACCCACCTCTCAACCACAGCCCACTCCTCCCAACAACATGACACCCTACTTACCCAGG
  ACTCAGACTACTGGCCCTGTGTCCCAGGGTAAGGCACCAGGCCAGGTGACCCCACCTACCCCACCTCAGA
  CTGCTCAGGCCCCCCTTCCAGGGCCTCCACCTGCAGCAGTAGAAATGGCAATGCAAATTCAGAGGGCAGC
  AGAGACACAGCGCCAGATGGCCCATGTACAGATTTTCCAAAGGCCAATCCAGCACCAGATGCCCCAGATG
  TCACCCATGGCCCCTATGGGCATGAACCCACCTCCTATGGCCAGAGGTCCTGGTGGGCATTTGGATCCAG
  GAATAGGTCCAGCAGGAATGCAGCAACAGCCACCTTGGGCCCAAGGAGGAATGCCTCAGCCCCAGCAGAT
  GCAGTCAGGGATGCCAAGGCCAGCCATGATGTCAGTGGCCCAGCATGGTCAGCCTTTAAACATGGCTCCA
  CAACCAGGATTGGGCCAAGTGGGTGTGAGCCCTCTCAAGCCAGGCACTGTGTCTCAACAAGCCTTACAAA
  ACCTTTTGCGGACTCTCAGGTCTCCCAGTTCTCCCTTACAGCAGCAACAGGTGCTTAGTATCCTTCATGC
  CAACCCCCAACTGTTGGCTGCATTCATCAAGCAGCGGGCTGCCAAGTATGCCAACCCTAATCCACAGCCT
  CTCCCTGGACAGCCTGGCATGACCCAGGGACAGCCAGGGCTGCAGCCACCTACCATGCCTGGCCAGCAAG
  GTGTCCACTCCAACCCAGCCTTGCAGAACATGAATCCTCTGCAGGCAGGTGTCCAGAGAGCTGGCCTGCC
  CCAGCAGCAGCCGCAGCAGCAGCTCCAGCCACCCATGGGAGCAATGAGTCCCCAAGCTCAGCAAATGAAC
  ATGAATCACAATACCATGCCTTCACAGTTCAGAGACATCTTAAGACGGCAGATGATGCAACAGCAGGGAG
  CAGGGCCAGGAATCGGCCCTGGAATGGCCAACCAGTTCCAGCAGCCCCAAGGAATTGGCTATCCACCGCA
  GCAGCAGCAGCAGCAGCGAATGCAGCATCACATGCAGCAAATGCAGCAAGGAAATATGGGACAAATGGGT
  CAGCTTCCCCAGGCTCTGGGGGCTGAGGCAGGAGCCAGTCTACAGGCCTATCAGCAGCGACTTCTTCAAC
  AACAGATGGGGTCTCCTGCTCAGCCCAACCCTATGAGCCCACAGCAGCACATGCTCCCAAATCAGGCACA
  GTCCCCACACCTACAAGGTCAACAAATCCCTAATTCTCTCTCCAATCAAGTGCGCTCTCCCCAGCCTGTC
  CCTTCTCCTCGACCACAGTCTCAGCCCCCCCATTCCAGCCCATCTCCGAGGATGCAGCCTCAGCCTTCTC
  CACACCATGTTTCTCCACAGACCAGTTCCCCACATCCTGGACTGGTAGCTGCCCAGGCTGCCAACCCTAT
  GGAACAAGGGCATTTTGCCAGCCCAGACCAGAATTCAATGCTTTCACAGCTCGCTAGCAATCCTGGCATG
  GCAAACCTCCACGGGGCAAGTGCCACGGACCTGGGACTGAGCAGCGATAATGCAGACTTGAATTCAAACC
  TCTCACAGAGTACACTAGACATACACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177821
• Insert Size: 7239 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177821.6, NP_808489.4
• RefSeq Size: 8749 bp
• RefSeq ORF: 7239 bp
• Locus ID: 328572
• UniProt ID: B2RWS6
• Cytogenetics: 15 E1
• Gene Summary: Functions as histone acetyltransferase and regulates transcription via chromatin remodeling (By similarity). Acetylates all four core histones in nucleosomes (By similarity). Histone acetylation gives an epigenetic tag for transcriptional activation (By similarity). Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein (PubMed:18486321, PubMed:24216764). Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability (By similarity). Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac) (By similarity). Also functions as acetyltransferase for non-histone targets, such as ALX1, HDAC1, PRMT1 or SIRT2 (PubMed:28883095). Acetylates 'Lys-131' of ALX1 and acts as its coactivator (By similarity). Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function (By similarity). Acetylates HDAC1 leading to its inactivation and modulation of transcription (By similarity). Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2 (By similarity). Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium (By similarity). Promotes cardiac myocyte enlargement (By similarity). Can also mediate transcriptional repression (By similarity). Acetylates FOXO1 and enhances its transcriptional activity (By similarity). Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity (By similarity). Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter (By similarity). Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed:14645221, PubMed:9512516). Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (By similarity). Acetylates MEF2D (By similarity). Acetylates and stabilizes ZBTB7B protein by antagonizing ubiquitin conjugation and degragation, this mechanism may be involved in CD4/CD8 lineage differentiation (PubMed:20810990). In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E)-butenoyl-CoA (crotonyl-CoA), butanoyl-CoA (butyryl-CoA) or propanoyl-CoA (propionyl-CoA), and is able to mediate protein crotonylation, butyrylation or propionylation, respectively (PubMed:27105113). Acts as a histone crotonyltransferase; crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Histone crotonyltransferase activity is dependent on the concentration of (2E)-butenoyl-CoA (crotonyl-CoA) substrate and such activity is weak when (E)-but-2-enoyl-CoA (crotonyl-CoA) concentration is low (By similarity). Also acts as a histone butyryltransferase; butyrylation marks active promoters (PubMed:27105113). Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway (By similarity). Acetylates PCK1 and promotes PCK1 anaplerotic activity (By similarity).[UniProtKB/Swiss-Prot Function]