Frem1 (NM_177863) Mouse Untagged Clone

CAT#: MC225220

Frem1 (untagged) - Mouse Fras1 related extracellular matrix protein 1 (Frem1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Frem1
• Synonyms: BC037594; crf11; D430009N09; D630008K06; eye; eyes2; heb; qbrick
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225220 representing NM_177863
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTCTCCAGGCTGCACTGGGCCCAAGGCTCAGTGGTTCTTGCTGCTCCAGCTGCTTCTGCTGCACC
  TGGACCGGGTCAGTGCCACCTTCATCAGCATCAACCGGGGCCTGAGGGTGATGAAGGGTAGTTCTGCCTT
  CCTGTCTGGAGATCACCTGAGGGTTGCAGTCCCTAAAGAAAAGGACGCCTGCAGACTGGAGGTGGTGATG
  AACGAGCCCGTAACCCAGAGGGTTGGGAAACTCTCTCCACAGGTCTTTGATTGCCATTTTCTTCCCAACG
  AAGTGAAGTATGTTCACAATGGATGCCCAATTCTGGATGAAGACTCTGTGAAGCTTAGACTTTACAGGTT
  TACTGAAACAGACACCTTCATGGAAACCTTTCTCTTGCGGGTTTATCTTGTGGAACCAGACTGTAACATC
  ATCCGTATGAGCAGTAATGTGTTGGAAGTGACTGAATTCTATGGCTTATCGCAAGCCATTGATAAGAACC
  TGCTCCAGTTTGATTATGATAGGACTGCCAGCCTGGATTGCACCATCAGACTAGACCCACTGAGGACTCA
  GCTGCCAGCTCACGGCAAGCTGGTCGTGGTGAATCGTAAGTCAGAGGGGCCTCGTGGAGATCAGCCACAC
  AGCTTCTTCTCTGAGACAGAGCTAGGGGCTGGACTGAAGTGTCCAGATGGAAGCTGTGCCCTGGAATTGA
  AGCAAGTGGCAAGTCTGAAAGTGAGCTGTGAGGAGTTTCTGCTGACGGGGTTTCACTACCAACACATGCA
  GCCCCCTTCACCCAACATTGATTACATCCCCATTCAGTTGGATCTCACCGACAGAAGGAGCAAAACCGTA
  TACAAGTCAGAGAGTGCGTGGTTGCCTGTCTATATCAGAGTTGGCATTCCTAACCAGGTCCCAAGGGCTG
  CATTTATGGCCATGTTTGTTCTGGAGGTGGACCAGTTCATACTGACCCCTCTGACAACCTCCGTTCTGGA
  CTGTGAGGAGGATGAGACTCCGAAACCCTTGTTGGTGTTCAACATCACTAAAGCCCCACTCCAGGGCTAT
  GTGACCCACCTGCTGGATCATACCAGACCCATCTCTTCATTCACCTGGAAGGATCTCAGTGACATGCAAG
  TTGCCTACCAGCCACCAAACAGCAGCCACCCTGAGAGGAGACATTACACGATGGAGCTGGAGGTGTACGA
  CTTCTTCTTTGAGAGGAGTGCCCCCATCACTGTCCACATCTCCATCAGAACAGCAGACACGAATGCACCA
  CGGGTGTCTTGGAATACAGGTTTGAATCTCCTAGAGGGGCAGTCTCGGGCCATCACTTGGGAACAATTTC
  AGATCGTTGACAATGATGACATTGGTGCTGTGCAGTTGGTCACCATTGGTGGCCTGCAGCATGGACGGCT
  TACAGTACGAGAGGGGAAAGGATTTCTCTTCACCGTGACTGACCTTCAAGCTGGAGTTGTCCGCTATCAT
  CACGATGACAGTGATACCACCAAAGACTTTGTGGCTTTCAGGATATTCGATGGCCACCACAGCAGCCACC
  ACAAGTTTCCTATCAACATCTTGCCTAAAGATGACAGCCCCCCATTCCTCATCACCAATGTCGTGATTGA
  ACTAGAAGAGGGGAAGACCATCTTGATCCAAGGGTCTATGTTGAGAGCTTCAGATATGGACTCCAGTGAT
  GACTACATCTTCTTTAATATCACAAAGTTTCCACAGGCTGGGGAGATCATGAAGAAGCCAGGGCCAAGAC
  TGATAGGGTATCCTGTCCCTGGCTTCCTGCAGAGAGACCTGTTCAGTGGAATCATTTACTATCGTCACTT
  TGGTGGAGAAATCTTTGAAGATTCCTTTGAATTCGTACTGTGGGACAGCCATGAGCCTCCAAATCTCTCA
  GTGCCACAGGTGGTGACAATTCATATCACTCCAGTAGATGACCAGCTTCCTAAAGAAGCTCCTGGCATTT
  CCCGGCATTTGGTTGTCAAGGAAACAGAGGTGGCCTACATCACTAAAAAGCACCTACATTTCCTAGACAT
  GGAATCCCGTGATGGGGAACTCATCTACACAGTAACACGCCCTCCATGTTTCTCCTTCAGCCACAGACAC
  TTGGATGCTGGGAAGCTATTCATGGTGGACAGCATACCAAAGCTAACCAAAAATCCGACAGCCCCAGGGC
  TAAGTTCATTTACTCAGGGCTTGATTCTGATCTCTGCAAATCAGCACACGTGTTCATCTCAATTTGCTTC
  ACAGCATGCCGTGAACCATATGAAAGTGGCCTACATGCCACCCATGCAAGATATAGGCCCCTCCCCTCGA
  CATGTGCAGTTCACAGTGTCTGTCAGTAACCAGCATGGAGGTGCTTTGCATGGAATCTGCTTCAACATCA
  CAGTTCTCCCAGTGGACAACCAGGTTCCCGAGGTATTCACCAACACCCTGAGAGTGGTCGAGGGCGGTCA
  GTGCACCATCAGCACAGAGCACATTTTGGTTTCTGATGTGGACACCCCGCTAGACAGCATCAGCCTCTCT
  CTTAAGGAGAGGCCTCTGCATGGCGGGGTAGAGCTGGACGGATTTCCTCTAAACCCAAGAGGCACATTTT
  CTTGGCGAGACCTCAATACCTTAAAAGTTTGGTATCAACATGATGGATCTGAGGTTCTTCAGGATGAGAT
  ATTTTTGGAGGTCACAGATGGCACAAATTCAGCAGCATTTGTTCTCCACATTGAGGTGTTCCCTGTGAAT
  GATGAGCCACCCATTCTGAAGGCTGACCTCATCCCCATGATGCACTGCTCTGAGGGTGGAGAGGTGACCA
  TCACCCCTGAATACATTTCCGCTACTGATGCAGACAGCGATGACTTAGAACTGCTGTTTCTGATTGCCCG
  AGAGCCTCAGCATGGAGTGGTGAGAAAGGCTGGACTTCACGTGGACAGGTTCTCTCAGGGAGATGTCATC
  TCAGGGGCTGTGACATACAAACATACAGGTGGCGAGATTGGCCTTGAACCTTGTTCTGACACTGTTGTAT
  TGGTGGTTTCGGATGGTGAAGCTGACCCTTTGATGAACGGCTGTTGCTACGATGGACCTGATTCATCTGT
  TCCTCTTCATAAGTCCTTCCCCACATACCAGCTCAACATCACAGTACATCCAGTCGACAACCAGCCACCT
  TCAATTATAATAGGTAGGATGCTCACAGTGGATGAAGGCTTCTCAGCAGCCCTTACTACTCATCACTTGA
  CTGTTGTTGATTGGGACACTGCCCCGGATGACTTAAAATTCATGTTGGCTTCTCAGCCCCAGTTTGGCTA
  CTTAGAAAACGCACTGCCTTCTGCAGGCTTTGAGAAAAGTAACATCGGCATAAGGATAGCCTCATTCCAG
  TGGACAGACATGAAGGCTTCACACATTAACTATGTGCAGTCCAGGCACCTGAGAGTGGAACCAACTGCTG
  ACCAGTTCACAGTATATGCCACAGACGGGAAGCATCGCTCCTTGGAGACAACATTTCACGTTATCATCAA
  CCCCACAAACGATGAAGCTCCTGACCTTGCAGTACAGAACATCACTGTGTATGAGGGGCACATGGTAGAA
  CTGGATTCTTCCATCATCAGTGCTACTGACAGGGATATTCCCAAGGACCCCTTGCTCTTCAGCATCGCTC
  TCAAGCCCCAGCACGGCCTGCTCGTCGATGCTGCCATCAGCAAGGACTCCCATCAGATCAAGCAGCTGCA
  ACACGAAATCCACAGCTTCTCCGTGGACCTTCTCAAGAATGGAATGAAGCTGGTCTATGCCCACGACGAC
  TCAGAGAGCTCTGCTGATAACTTCGTGATCCAGTTGTCAGACGGGAAACATAAAATCCTTAAAACCATCT
  CAGTAAACATTACCCCAGTGAATGACGAGACACCAACGCTAAGCAAGAAGGCTGAAATCTCAATGGCTGT
  GGGCGATACTCGCGTCCTGTCTAGTGCTGTTCTTTCAGCCACAGATAAAGACTCGCCCAGAGAGAAGATT
  CACTATGTATTTGAAAGACTTCCCCAAAATGGACAACTTCAACTTAAGATAGGGAGGGACTGGGTCCCTC
  TCTCCACTGGCATGCAGTGCACCCAGGAGGATGTGGACCTGAACTTGCTAAGATACACGCATGCTGGTAA
  AACGGACTCCCAGGATGGAGACAGCTTCACCTTCTACCTCTGGGATGAGGACAACAGGTCACCTGCATTT
  GACTGTCACATCATCATTGAAGACATGGGCAAAGGTGATATTGTCATTCATGCAAAACCACTCGTGGTAG
  TCAAAGGTGACAGAGGTCTCTTGACGACTGCCACCCTTCTGGCTGTGGATGGAGCAGACAAGCCTGAGGA
  ACTGCTCTACCTCATCACCTCCCCACCACGGCATGGCCAAGTGGAGTACGTCCACTCTCCTGGAGTCCCC
  ATTGCCAGCTTCAGCCAAATGGACATAGCAGGACAGACAGTGTGCTACATACACAAGAGCAGGACAGCTG
  TCCCCACTGACAGCTTCAGATTTACCATCAGCAATGGACTGCAGACCCAGCGTGGGGTGTTTGAAATCAC
  ACTGCAGACTGTGGACAGCGCCTTGCCTGTGCTGACCAAGAACAAAAGGCTGAGGCTAGCCGAAGGGGCC
  ATGGGCCTCCTGTCCGCTGATCACCTTCAGCTGACTGACCCCGACACACCTCCAGAGAACCTGACCTTCT
  TTCTGGCTCAGCTCCCACGCCACGGGTACCTCTTCCTGAGAGGGAAAGCACTCCAACACAACTTCACCCA
  GCGAGATGTGGACAGCGGGGGCGTGGCCTACCAGCACTCAGGAGGCGGCGCCCGGGAGGACTATTTTACT
  TTTCTAGCCACAGACAGGAAGAACCAAGGCTTTGTTGTGGATGGGAAAGTTCAGAAAGAGCCTGTTCGCT
  TCACAATCCAGGTGGATCAGCTGGACAAGGCAGCGCCCCGTATCACACACTTGCACTCCCCTACTCAAGT
  GGGGCTCTTGAAAAATGGCTGCTATGGGATTTACATCACTTCCCGTGTGCTGAAGGCATCAGACCCTGAC
  ACAGAGGATGACCAGATCATCTTTAAGATTTTACGAGGCCCATTGTACGGACGTCTGGAGAACACAACAA
  CAGGTGAATTCATCCACGAGCGATTTAGCCAGAAGGACTTAAGCCATAAGACCATCCTTTACATCATAAA
  CCCTTCTTTGCAAGTGACTTCCGATATCCTGGAATTTCAGGCCATGGACCCCACAGGGAACACTGCCACT
  CCCCAGAGCTTGGACTTGAGATGGTCGTATATTGAATGGGCCCAGACTGCATATGAGGTTTGTGAGAATG
  TGGGCTTATTGCCCTTGGAAGTTACCAGAAGGGGATATCCCATGGACTCAGCCTTTGTGGGTGTAGAGGT
  TAACCAAGTGTCAGCTACAGTTGGAAAAGATTTTACTGTCACTCCATCCAAACTGCTTCAATTTGACCCA
  GGAATGTCAACTAAGATGTGGAATATAGCAATTACCTATGACGGATTAGAGGAAGATGATGAGGTCTTTG
  AAGTAATTCTGAACTCCCCTGTGAATGCGGTTCTTGGCACGCAGACAAAAGCGGCGGTGAAAATTTTGGA
  CTCAAAAGGAGGACGGTGCCATCCTTCAAATTCCTTCAACCAAAGCAAGCACAGCACGTGGGGGAAGGGC
  CCTTGGCATCCGCTGCCCTCAGGCTCATCCTCGCTCACCACTTCAGGTTCTCCTCTTCTGGAAAGGCCTC
  CTCCATCTTTCACCAGTGGAGATGCTCTTCAGGGTTTTGGTCTCACCGACCTCACTCAAAGGAAGACAAT
  GACCCAGGGGAATGGCAAATCAGTTCTCCCATCCTCTGTTTGTAGAAATGGGACAGACACCATCTACAAT
  TACCATGGTATAGTTTCCTTGAAACTAGAGGGCGACAGATTCTCAGCTCACAAACGGAAGGCCAAAATAT
  CCATTGTTAGTCAGCCACAGAGGACAATCAAGGTGGCAGAGCTGCCTCTAGCTGATAAGGTGGAATCCAC
  AACTGATTTGCACTTTCTCAGACAGGGTCTGCGGCCCTTGTTTCCAAAGAATTGTTCTGTGGACTTAAAG
  GGACTCTTTCATTTTGAAGAAAGCACCCACAGATTGTATCAATGTGATGGGATCTCCTGGAAAGCCTGGA
  GCCCCCAAACCAAGGGGCTGGAGGACAGATCCTGCCCAGGTGGATGGCTCCTTCATTCTGGCTACTGTCA
  CATCTTGGTCACAAGACAGAAAGGCACCTGGACCACAGCTACCCGAGCTTGCAGAGAACAGCACCAGGGC
  GACCTTGTGACCGTACTCTCCAGAAGGCATATGCAGTGGCTATGGGCGATGAGTGGAAGAAAACCCTTCT
  GGATAGGCTTGAAGAACCAACCGAGGACTGGCCACTGGGAGTGGATCGGAGGTGAACCTGTTGCTTTTAC
  CAACTGGAGGAGAGGGGCCCCTCTGCACCCAAAACCTGGAAAGAACTGTGCTTTGGTTCAGAAAAGAGGA
  CAATGGCAGACAAAGAACTGTAGCAAAGGCAAGGCTCACAATTTTGTGTGCTCAAGGAAACTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177863
• Insert Size: 6576 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177863.4, NP_808531.2
• RefSeq Size: 9307 bp
• RefSeq ORF: 6576 bp
• Locus ID: 329872
• UniProt ID: Q684R7
• Cytogenetics: 4 C3
• Gene Summary: Extracellular matrix protein that plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.