Med13 (NM_001080931) Mouse Untagged Clone

CAT#: MC225210

Med13 (untagged) - Mouse mediator complex subunit 13 (Med13), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Med13
• Synonyms: 1110067M05Rik; D030023K18; Thrap1; Trap240
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225210 representing NM_001080931
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCCTCCTTCGTGTCGAACGGGGCCAGCCTGGAAGATTGTCACTGTAACCTCTTCTGCCTGGCCG
  ACTTGACAGGAATTAAGTGGAAAAGATATGTATGGCAAGGCCCAACTTCCGCCCCTATTCTGTTTCCGGT
  GACAGAAGAAGACCCCATTTTGAGCAGTTTTAGTCGATGCCTTAAGGCAGATGTACTTGGTGTTTGGCGG
  CGAGATCAAAGACCTGGAAGAAGAGAATTGTGGATATTTTGGTGGGGTAAAGATCCCAATTTTGCTGACC
  TTATTCACCATGACTTATCAGAAGAGGAAGATGGAGTATGGGAGAATGGCCTTTCCTATGAATGCCGTAC
  TTTGCTTTTCAAAGCAGTTCACAATCTGTTGGAGCGATGTTTGATGAACCGAAACTTTGTACGAATTGGC
  AAGTGGTTTGTGAAGCCATATGAGAAAGATGAAAAACCTATAAATAAAAGTGAACACTTGTCCTGCTCTT
  TCACCTTTTTCTTGCATGGAGACAGCAATGTTTGTACCAGTGTGGAAATTAACCAACATCAACCTGTATA
  CCTTCTCAGTGAAGAGCATGTCACCCTTGCTCAACAGTCTAATAGCCCATTTCAAGTTATCTTAAGTCCA
  TTTGGACTAAATGGCACTCTCACGGGACAGGCTTTCAAGATGTCTGATTCAGCTACGAAAAAATTAATTG
  GTGAATGGAAACAGTTCTATCCTATTTCATGTGGTTTGAAGGAGATGTCTGAGGAGAAACAGGACGATAT
  GGATTGGGAAGACGATTCTTTGGCTGCGGTGGAAGTTCTTGTTGCGGGTGTCCGAATGATCTACCCAGCA
  TGCTTTGTGCTAGTCCCTCAGTCAGACATTCCTGCTCCTAGCTCTGTGGGAGCTTCTCACTGTTCAGCTT
  CTTGCTTGGGTATCCATCAAGTGCCTGCTTCCACAAGAGATCCTGCTATGTCTTCAGTTACTCTCACTCC
  ACCTACATCTCCTGAGGAAGTCCAAACAGTTGATCCTCAGTCCGCACAGAAGTGGGTCAAATTTTCTTCA
  GTATCCGATGGCTTCAGCACTGACAGTACTAGCCATCATGGTGGGAAAATACCCAGAAAATTAGCAAATC
  ATGTGGTGGATAGAGTCTGGCAAGAATGTAATATGAACAGATTACAGAACAAGAGGAAGTATTCTGCTAC
  ATCCAGCGGCCTGTGTGAGGAAGAGACTGCCGATAAAATAGGATGCTGGGACTTTGTTGAAGCCACACAA
  AGAACAAGCTGCAGTTGTTTGAGGCATAAAAGTCTCAAGACAAGAAATACTGGGCAACAAGGACAGGCAC
  CATCTTTAGGGCAGCAACAGCAAGTACTTCCTAAGCACAAGACCAATGAGAAGCAAGACAAGAGTGAGAA
  GCCACAGAAGCGCCCCTTGACTCCCTTTCACCATCGTGTATCAGTTAGTGATGAGATTGGCATGGACACA
  GATTCAGCCAGCCAAAGACTTGTGATCTCTGCTGCAGATAGTCAAGTGAGGTTTTCAAACATCCGAACTA
  ATGATGTAGCAAAGACTCCTCAGATGCATGGCACTGAACTGGCAAATTCACCTCAGCCTCCCCCACTTAG
  TCCTCATCCTTGTGATGTGGTTGATGAAGGAGTGACTAAAACACCTTCAACTCCGCAGAGTCAACATTTT
  TATCAAATGCCAACACCGGATCCCTTGGTTCCTACCAAACCAATGGAAGATAGGATAGACAGTTTGTCCC
  AGTCTTTCCCACCTCCATTTCAGGAAGCTGTAGAACCTACCGTGTATGTTGGTACAGCAGTAAGCTTGGA
  AGAAGATGAAGCTAATGTAGCCTGGAAGTATTACAAAGTCCCAAAGAAAAAAGATGTAGAATTTTTACCA
  CCTCAACTTCCAAATGATAAATTCAAGGATGATCCAGTTGGACCTTTTGGACAGGAAAGTGTAACATCAG
  TTACAGAGTTAATGGTGCAGTGTAAGAAGCCTTTAAAAGTTTCTGATGAAATAGTCCAGCAATATCAAAT
  TAAAAATCAGTATCTTTCAGCAATAGCATCTGATACAGAACAAGAACCTAAAATTGATCCATATGCATTT
  GTTGAAGGAGATGAGGAATTCATTTTTACTGATAAAAAAGATAGACAAAATAGCGAGAGAGAAGCTGGGA
  AGAAACACAAGGTGGAAGATGGGACATCTGCTGTCACAGTGTTATCACACGAAGAAGATGCTATGTCATT
  ATTTAGTCCCTCTAAACAAGATGCTCCACGCCCTACAAATCATGCCCGTCCTCCATCAACCAGTTTGATT
  TATGACTCAGATTTGGCTGTGTCTTACACTGACCTTGATAACCTCTTCAATTCTGATGAAGATGAATTAA
  CACCTGGATCTAAAAAGTCAGCAAGTGGATCAGATGATAAAGCCAGCTCCAAAGAATCAAAGACAGGAAA
  TCTGGATCCGTTGTCCTGCATAAGCACTGCAGACCTCCACAAAATGTACCCCACGCCACCCTCACTGGAA
  CAGCACATTATGGGCTTCTCTCCAATGAATATGAATAACAAAGAATACGGTAGTGTGGACACAGCACCCG
  GAGGGACTGTCCTGGAAGGAAATAGCTCTAGTGTAGGAACGCAGTTCAGAATCGAGGTCGAGGAAGGATT
  CTGTAGCCCTAAACCGTCTGAAATTAAAGATTTTTCTTATGTCTATAAGCCCGAAAATTGTCAAGTTCTA
  GTAGGATGTTCCATGTTTGCACCTTTAAAAACTCTACCAAGCCATTGTCTGCCTCCTATCAAATTGCCAG
  AAGAGTGTGTTTACCGGCAGAGCTGGACTGTTGGAAAATTGGACTTGCTTCCTTCAGGCCCTTCAATGCC
  ATTCATCAAGGAGGGTGATGGCAGTAATTTGGATCAGGACTATGGCCCCGCCTACACACCGCAAACCCAT
  GCTTCTTTTGGGATGCCTCCGAGCAGCGCACCTCCTAGTAATGGTGGTGCAGGAATTCTTCCTTCCCCAT
  CCACCCCTAGGTTTCCAACTCCAAGGACTCCAAGGACTCCAAGGACCCCTCGTGGGGCTGGTGGGCCTGC
  TAGTGCTCAAGGTTCAGTCAAGTATGAGAATTCAGACTTGTATTCACCAGCTTCCACCCCATCTACATGC
  CGACCCCTCAATTCTGTTGAACCTGCAACTGTTCCTTCCATCCCTGAAGCGCACAGTCTTTATGTAAACC
  TCATCCTTTCAGAATCAGTAATGAATTTGTTTAAAGACTGTAATTTTGATAGTTGCTGTATTTGTGTCTG
  CAATATGAATATCAAGGGTGCCGATGTTGGAGTTTACATTCCAGACCCAACCCAAGAAGCACAGTATCGG
  TGCACCTGTGGCTTCAGTGCTGTCATGAACCGAAAATTTGGAAATAATTCAGGATTATTTCTTGAAGATG
  AACTTGACATCATAGGACGTAACACAGACTGTGGGAAAGAAGCAGAAAAACGTTTTGAAGCTCTCAGGGC
  TTCCTCTGTGGAGAATGTTAATGGAGGCCTGAAGGAATCTGAAAAGGTGCCTGATGAATTAATATTACTG
  CTACAAGATCAATGCACTAATTTATTTTCACCCTTTGGAGCAGCAGACCAAGATCCTTTTCCCAAAGTGG
  GTATAAGTAGCAATTGGGTGCGTGTTGAGGAGCGGGACTGTTGTAGTGACTGCTGCCTCGCCTTAGAACA
  TGGGCGTCAGTTCATGGATAATATGTCAGGAGGGAAAGTTGATGAAGCACTTGTCAGAAGCTCATGCTTA
  CATCCGTGGGCCAAGCAAAATGATGCGAGCGTGCAGTGTTCACAGGATATACTTCGGATGCTCCTTTCTC
  TTCAGCCAGTTCTTCAAGATGCTATTCAGAAAAAAAGAACAGTGAGGCCTTGGGGTGTTCAAGGTCCTCT
  CACATGGCAACAATTTCATAAAATGGCTGGCCGAGGCTCTTATGGAACTGATGAATCTCCGGAACCACTG
  CCGATTCCCACGTTTTTGTTGGGTTATGATTACGATTTTCTGGTGCTTTCTCCATTTGCTCTCCCTTACT
  GGGAGAAACTTATGCTGGAACCCTATGGATCTCAAAGAGATATAGCCTATGTTGTACTCTGTCCAGAGAA
  TGAAGCTTTGTTAAATGGAGCCAGAAGCTTTTTTAGAGATCTCACTGCAATATATGAGTCCTGTCGATTA
  GGCCAACACAGACCTATTTCTCGATTGTTGACGGATGGGATCATGAAAGTGGGAGCTACTGCATCAAAGA
  AACTTTCAGAAAAGTTTGTAACAGAATGGTTTTCTCAGGCAGCTGATGGTAACAATGAAGCATTTTCCAA
  ATTGAAGCTGTATGCACAAGTCTGCAGATATGATTTAGGTCCATATCTTGCTTCTCAGCCGTTGGACAGC
  TCTCTGCTCTCCCAGCCAAATTTAGTTGCCCCTCCAAATCAGTCTTTGGTTACTGCACCTCAGATGACAA
  ATACTGGAAACGCTAATGCTCCATCTGCCACCTTAGCATCTGCGGCAAGTAGCACTATGACAATGACTTC
  AGGTGTTCCCATATCTACTTCAGTTGCCACAGCTAATTCAACTTTGACCACAACTTCATCTTCATCATCT
  TCCAGTCTGAGTAGTGGAGTATCATCAAATAAACTGCCTTCGTTTCCACCCTTTGGCAGTATGAACACTA
  GTGGTACAGGATCCATGTCTGCACAAGCAAGTACGGTTCAGAGTGGCCAGCTAGGAGGACAGCAGTCGTC
  ATCTCTGCAGGCAGCAGGAATATCAGGGGAGTCAGCTTCTCTTCCCACTCAGCCACATCCTGATGTGTCT
  GAAAGCACAATGGATCGTGATAAAGTGGGGATTCCCACAGATGGTGATTCACATGCAATTACCTACCCTC
  CTGCAATTGTTGTTTATATAATCGATCCTTTTACATATGAAAATAAAGACGAGAGCACTAACTCTTCTAA
  TGTATGGACTTTGGGGCTCCTTCGATGCTTCTTGGAAATGGTCCAAACTCTTCCTCCTCATATCAAGAGC
  ACTGTTTCTGTGCAGATTGTTCCTTGTCAGTACCTGTTACAACCTGTGAAACACGACGACAGACAGATCT
  ACTCCCAGCATTTAAAATCTTTGGCTTTTTCGGTGTTTACCCAGTGTCGGAGACCACTTCCAACATCAAC
  CAATGTAAAAACACTGACTGGGTTTGGTCCAGGCTTAGCTATGGAAACTGCTCTTAAAAGTCCTGATAGA
  CCAGAGTGTATTCGACTTTATACACCACCTTTTATTCTGGCTCCGGTGAAGGACAAACAAACAGAGCTAG
  GAGAGACCTTTGGAGAAGCTGGACAGAAATACAATGTTCTTTTTGTGGGCTACTGTTTATCACATGATCA
  AAGGTGGATTCTTGCATCTTGCACAGATCTATATGGAGAACTCCTAGAAACATGTATCATTAACATCGAT
  GTTCCAAATAGGGCCCGTCGGAAAAAGGGTTCTGCTAGAAGATTTGGTCTACAGAAACTGTGGGAGTGGT
  GCTTGGGACTTGTTCAGATGAGCTCTTTACCATGGAGAGTTGTGATTGGTCGCCTGGGAAGGATTGGACA
  TGGAGAGTTGAAAGATTGGAGTTGTTTGCTGAGTCGTCGAAACTTGCAATCTCTAAGCAAAAGGCTCAAA
  GACATGTGTAGGATGTGTGGCATATCTGCTGCAGACTCTCCAAGTATCCTTAGTGCTTGTTTGGTTGCAA
  TGGAACCCCAAGGTTCTTTTGTTATCATGCCAGATTCTGTGTCAACTGGTTCTGTTTTTGGAAGAAGTAC
  CACTCTAAATATGCAGACGCCTCAGCTAAATACCCCACAAGATACATCCTGTACTCATATACTTGTGTTC
  CCTACTTCTGCTTCTGTGCAAGTAGCTTCAGCTACTTACACCACTGAAAATTTGGATTTAGCTTTCAATC
  CCAACAATGATGGAGCAGACGGAATGGGTATCTTTGATTTATTAGACACAGGAGATGATCTTGATCCTGA
  TATCATTAATATCCTTCCTGCATCTCCAACTGCATCTCCTGTACATTCTCCAGGCTCTCATTACCCCCAT
  GGAGGTGATGCTGGCAAGGGTCAGGGTACTGATCGGCTACTTTCAACAGAATCGCATGATGAAGTAACTA
  ATATTCTTCAACAACCATTGGCCCTTGGATACTTTGTATCAACTGCCAAAGCGGGTCCATTACCTGACTG
  GTTCTGGTCAGCATGCCCTCAAGCACAATATCAATGCCCCCTTTTTCTTAAGGCCTCTTTGCACCTCCAC
  GTGCCTTCAGTGCAATCTGACGAGCTGCTTCACAGCAAACACTCCCACCCACTGGACTCAAATCAGACTT
  CAGATGTCCTCAGGTTTGTTTTGGAACAGTACAATGCACTCTCATGGCTAACCTGTGACCCTGCAGTCCA
  GGACAGACGCTCATGTCTCCCAGTTCATTTTGTGGTGCTGAATCAGTTATATAACTTCATCATGAATATG
  CTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001080931
• Insert Size: 6516 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080931.1, NP_001074400.1
• RefSeq Size: 11787 bp
• RefSeq ORF: 6516 bp
• Locus ID: 327987
• UniProt ID: Q5SWW4
• Cytogenetics: 11 C
• Gene Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).[UniProtKB/Swiss-Prot Function]