Pkd1l3 (NM_181544) Mouse Untagged Clone

CAT#: MC225202

Pkd1l3 (untagged) - Mouse polycystic kidney disease 1 like 3 (Pkd1l3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pkd1l3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225202 representing NM_181544
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTTGCAGAGGCGGTCCTGGCTCTGGCTGTACATTAGAATCGGTGTCATTCTGGGTGATATTTTGG
  GACGTAAACCAAGCATCCGGGAGCAACATGGGGGAAACAGCTGCTATCAGCTTAACAGACTTTTCTGTGA
  CTTCCAGGAAGCAGATAACTACTGCCACGCCCAGAGAGGACGCCTAGCCCACACGTGGAACCCCAAGCTT
  CGGGGTTTCCTAAAAAGCTTCCTGAATGAAGAAACAGTGTGGTGGGTCAGGGGAAACCTGACGCTGCCCG
  GATCGCATCCAGGGATAAATCAGACAGGAGGTGATGACGTCTTAAGGAACCAAAAGCCTGGCGAGTGCCC
  TTCCGTGGTCACACACTCTAATGCTGTCTTCTCAAGATGGAACCTGTGCATAGAGAAGCATCATTTCATT
  TGCCAGGCTGCCGCCTTTCCCCCTCAAGGTGCAAGCATTTGGAGAAATGAGTTTGGTCCTGGTCCTCTGT
  TACCCATGAAAAGAAGAGGAGCTGAGACAGAGAGACATATGATCCCAGGAAATGGCCCCCCGTTAGCCAT
  GTGTCACCAACCCGCTCCTCCTGAGCTTTTTGAGACATTGTGCTTTCCCATTGACCCAGCTTCTTCAGCA
  CCTCCAAAAGCCACACACAGGATGACAATCACATCCCTAACTGGAAGGCCACAGGTGACATCAGACACAC
  TTGCATCCAGCAGCCCACCACAGGGGACATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATC
  AGCCACATCTGCATCTAGCAGCCCACCACAGGGGACATCAGACACACCTGCATCCAGCAGCCCACCACAG
  GTGACATCAGCCACATCTGCATCTAGCAGCCCACCACAGGGGACATCAGACACACCTGCATCCAGCAGCC
  CACCACAGGTGACATCAGCCACATCTGCATCTAGCAGCCCACCACAGGGGACATCAGACACACCTGCATC
  CAGCAGCCCACCACAGGTGACATCAGCCACATCTGCATCTAGCAGCCCACCACAGGGGACATCAGACACA
  CCTGCATCCAGCAGCCCACCACAGGGGACATTAGACACACCTTCATCTAGCAGCCCACCACAGGGGACAT
  CAGACACACCTGCATCCAGCAGCCCACCACAGGGGACATCAGAGACACCTGCATCCAACAGCCCACCACA
  GGGGACATCAGAGACACCTGGATTCAGCAGCCCACCACAGGTGACAACAGCCACACTTGTATCCAGCAGC
  CCACCACAGGTGACATCAGAGACACCTGCATCCAGCAGCCCAACACAGGTGACATCAGAGACACCTGCAT
  CCAGCAGCCCAACACAGGTGACATCAGACACACCTGCATCCAATAGCCCACCACAGGGGACATCAGACAC
  ACCTGGATTCAGCAGCCCAACACAGGTGACAACAGCCACACTTGTATCCAGCAGCCCACCACAGGTGACA
  TCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGACACACCTGCATCCAGCAGCCCACCAC
  AGGTGACATCAGAGACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGACACATCTGCATCCATCAG
  CCCACCACAGGTAATATCAGACACACCTGCATCCAGCAGCCCACCACAGGTGACATCAGAGACACCTGCA
  TCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACA
  CACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCACCATGGCCTGT
  TATAACAGAGGTCACCAGGCCTGAATCCACAATACCTGCTGGAAGATCTTTGGCAAACATCACTTCAAAG
  GCACAGGAAGACTCTCCCCTGGGAGTCATCTCTACCCATCCACAGATGTCATTTCAGAGTTCAACCAGTC
  AGGCCTTGGATGAGACAGCAGGGGAACGGGTCCCAACAATTCCTGATTTCCAAGCCCACAGTGAATTCCA
  GAAAGCTTGTGCCATCCTCCAGAGACTGAGAGACTTCCTGCCGACTTCTCCCACATCAGCTCAGGTCAGT
  GTGGCCAATTTACTCATTGACCTGAGTGAGCAGTTGCTGGTGCTCCCGTTTCAGAAGAACAACAGTTGGA
  GCTCTCAAACTCCAGCAGTCAGCTGCCCCTTCCAGCCTCTTGGACGTCTAACAACAACGGAAAAAAGCAG
  TCATCAGATGGCTCAGCAAGACATGGAACAGGTTGAAGACATGCTGGAGACATCCCTGATGGCCCTGGGG
  GAGATCCACAGAGCATTTTGCCAGCAGAGTCTGTGCCCTCAGTCAGCAGTGACCCTGGCCTCTCCCTCTG
  CTACTCTGATGTTGAGCAGCCAAAATGTGTCAACGTTGCCCCTGAGCACCTACACTTTGGGTGAGCCTGC
  ACCCTTGACTTTGGGCTTCCCGTCAGCAGAAGCTCTGAAGGAGCTCTTGAACAAACACCCAGGCGTGAAC
  CTTCAAGTGACAGGTCTGGCTTTCAACCCTTTTAAGACTTTGGATGACAAGAACATTGTTGGAAGCATTG
  GAAATGTGCAGCTGAGCTCTGCTTATCAGTCGATCAGAGTCCACGACTTAATAGAAGATATTGAGATCAT
  GCTCTGGAGAAATGCCAGCATGGAGACCCAGCCCACCAGCCTCAACACAAGTACAGACCATTTCACAATC
  TCTGTGAACATCACTTCCTTGGAGAAGACCCTCATTGTGACCATCGAGCCTGAAAGTCCCCTCCTAATGA
  CGCTCCACTTGGGCTTCCAGGACCAGCTGGCCCACACTCACTTCTATCTCAACATCAGCCTGCCAAGGGA
  CCAAGTGTGGCAGAAAGATGAGGAGTACACGTGGGTGCTGACACCAGAGAACCTGTGGTACGGGACTGGC
  ACCTACTACATAATGGCTGTGGAGAATAAAAGTACAGAGGCGGCACAGCACACACCCGTCCTGGTCTCAG
  TGGTCACAGCTGTCACCCAGTGCTATTTCTGGGACCGATACAATAGGACATGGAAGAGCGATGGATGCCA
  AGTGGGGCCGAAGAGCACCATTTTAAAGACACAGTGTCTCTGTGACCACCTGACCTTCTTCAGCAGCGAC
  TTCTTCATCGTGCCGAGGACGGTGGATGTAGAAAACACCATCAAACTGCTTCTTCATGTGACCAACAACC
  CTGTCGGGGTGTCATTGCTGTCCAGCCTCCTAGGATTCTATATCCTCTTAGCCATGTGGGCTTCCAGAAA
  GGATCGAGAAGATATGCAGAAGGTGAAGGTAACAGTCCTGGCTGACAATGACCCCAGCTCTGCATCCCAC
  TACCTTATCCAGGTCTACACTGGCTATCGGAGGAGGGCTGCTACCACCGCCAAGGTCGTTATCACTCTCT
  ATGGCTCAGAGGGGCACAGTGAGCCCCACCACCTTTGTGACCCTGAGAAGACAGTTTTTGAGCGTGGAGC
  ACTGGATGTTTTCCTTCTTTCCACCGGATCCTGGCTGGGGGACCTGCATGGCCTTCGGCTGTGGCATGAC
  AATTCTGGCGACAGCCCTTCTTGGTATGTAAGCCAGGTGATCGTCAGTGACATGACCACGAGGAAGAAAT
  GGCATTTCCAGTGCAATTGTTGGCTGGCCGTGGACTTGGGCAACTGTGAGCGTGACAGGGTGTTCACACC
  AGCCTCCAGAAGCGAGCTCTCTTCCTTCAGACACCTGTTCTCCTCCACAATCGTAGAAAAGTTCACCCAG
  GATTATCTGTGGCTCTCAGTTGCAACTCGACATCCCTGGAACCAGTTTACACGAGTCCAGAGGCTCTCCT
  GCTGCATGGCACTACTGCTCTGTGACATGGTCATCAATATTATGTTCTGGAAGATGGGTGGCACCACTGC
  CAAGAGGGGCACCGAACAACTAGGTCCACTTGCTGTGACCTTGTCGGAGCTGCTCGTCAGCATCCAGACC
  TCCATCATCCTCTTCCCCATCCACCTCATCTTTGGGCGGCTCTTCCAGTTGATTCACCCACCAGAAGCTC
  TGCCCCAGCTTCCTTTCATCCAGGCTGCCTGGCCCCCTGCTCTTGTTTGTGAGTCCCCCTCTCTTACACA
  GGTGGTCAAGGAATTAAAGGAAACTGTGGGATTCCTGCTCAGGAGAAATACACAGCTGCTCTCGGAGTGT
  GAGCCGTCTTCGTGCAGTTCTTGTGACATTAACAAGCTGGCGAAGCTTTTATCCGGCCTCATTTACTGTC
  ACTTAGAAGACGAAGGCTGTCACCAGCAGACAGAATCCCACTGGGAAGACGCAGTGTCTGAAAACCATTA
  CCATTTCTGCCGCTACCTTCTCCAACTTCTGCGGAGACTGAAAGCGCATTTAGAGGCTCTTGGTGCTACC
  CAGGATCACCAGTCTTGTGATTTCTCAGAAGCAGTCAGCCAACTTCAAAACCTCCAGGAACTCCTGGAGA
  CACAGACTCTCCGCAGAGGGCCAGGGCCATGCAGGCATTCCACCAGTTTCCCCATCCTCAGCCCAGGAGA
  AGGGAAGAAGCCCATGTCATTTTGCCTGTTCAGATGGTTGAAGTGCAGCTGCTGGCTCCTTCTTGGTGTC
  ATCAGCCTGGCCTCGGCCTTTTTTATAACGCTCTATAGCTTGGAGTTGGACAAAGACCAAGCCACCAGCT
  GGGTTATTTCAATGATGCTGTCGGTACTACAAGACATCTTTATCAGCCAGCCGATAAAGGTCATCTTCCT
  GACATTGTTGTTCTCCCTGATGGCAAACCACATGCCGTGGCTTAACAAAGACAAGGAACAACACGCCCGG
  AGAATCGTAGCACTTTGGGCAAAGTGTCCTTGGTCGGCACCTGGCTTGAGAGACAAGAACAATCCCATCT
  ACACTGCCCCAGCAATGAACAACCTAGCCAAGCCTACAAGGAAGGCCTGGAAGAAGCAGCTCTCCAAGCT
  GACGGGTGGTACTCTGGTGCAAATCCTCTTCCTGACCCTGCTGATGACTACCGTCTATTCTGCAAAGGAC
  TCTAGTCGATTTTTCCTCCATCGAGCTATCTGGAAGAGGTTTTCTCACCGTTTCTCAGAAATCAAAACTG
  TAGAGGATTTCTACCCCTGGGCCAACGGCACCCTCCTTCCTAACCTATATGGGGATTACAGAGGATTTAT
  TACTGACGGGAACTCCTTTCTTCTGGGCAATGTTTTGATCCGCCAGACTCGCATTCCTAATGACATATTC
  TTCCCAGGATCTCTCCACAAGCAAATGAAGTCGCCTCCCCAACATCAGGAGGACAGAGAGAACTATGGGG
  CTGGCTGGGTCCCCCCTGACACAAACATCACAAAAGTAGACAGTATTTGGCATTATCAGAATCAGGAGTC
  GCTGGGAGGCTATCCCATCCAAGGGGAGCTAGCCACTTACTCAGGAGGAGGCTATGTTGTGAGGCTTGGA
  AGAAACCACAGTGCGGCAACCAGGGTTCTGCAGCATCTGGAACAGAGGCGCTGGCTGGACCACTGCACAA
  AAGCCCTCTTTGTAGAATTCACGGTCTTCAATGCTAATGTGAATCTGCTCTGTGCGGTGACCCTCATCTT
  GGAATCCAGTGGTGTGGGGACTTTCCTCACCTCCCTGCAACTGGACAGTTTAACTTCCCTTCAGTCATCA
  GAGAGGGGCTTCGCCTGGATCGTCTCACAGGTCGTCTACTACCTTCTCGTCTGTTACTATGCCTTCATCC
  AGGGCTGTCGGCTGAAGCGGCAGAGGCTGGCGTTCTTCACTAGGAAAAGGAACCTCCTGGACACAAGCAT
  CGTCCTCATTAGCTTCAGCATCCTGGGCCTCAGCATGCAGAGCCTCTCTCTACTTCACAAAAAGATGCAG
  CAGTACCACTGTGACCGGGACAGGTTCATCAGTTTCTACGAGGCACTGAGAGTGAACTCTGCAGTCACCC
  ACCTCAGGGGCTTCCTGCTTCTCTTCGCAACTGTGCGGGTCTGGGACCTACTGCGACATCATGCCCAGTT
  ACAGGTCATCAACAAGACACTGTCCAAAGCCTGGGACGAGGTGCTGGGCTTTATACTGATCATCGTGGTC
  CTGTTAAGCAGCTATGCCATGACTTTCAACCTGCTGTTTGGATGGAGCATCTCTGACTACCAGAGCTTCT
  TCAGATCTATAGTGACTGTTGTTGGCCTCTTGATGGGAACTTCAAAGCACAAGGAGGTTATTGCTCTATA
  CCCAATCCTGGGCTCCCTTTTGGTTCTCAGTAGCATCATCTTGATGGGACTTGTGATCATTAATCTTTTT
  GTTTCTGCCATTCTCATTGCCTTTGGGAAAGAAAGGAAGGCCTGTGAGAAAGAAGCTACACTGACAGATA
  TGTTACTACAAAAGCTCTCAAGTCTGTTAGGAATCCGCCTGCACCAGAATCCATCTGAGGAACACGCTGA
  CAACACTGGGTATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_181544
• Insert Size: 6456 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181544.2, NP_853522.2
• RefSeq Size: 6456 bp
• RefSeq ORF: 6456 bp
• Locus ID: 244646
• UniProt ID: Q2EG98
• Cytogenetics: 8 D3
• Gene Summary: Component of a calcium channel. May act as a sour taste receptor by forming a calcium channel with PKD1L3 in gustatory cells; however, its contribution to sour taste perception is unclear in vivo and may be indirect.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has multiple differences in the coding region and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a shorter protein (isoform b) that has a distinct C-terminus, compared to isoform a.