Dock8 (NM_028785) Mouse Untagged Clone

CAT#: MC225172

Dock8 (untagged) - Mouse dedicator of cytokinesis 8 (Dock8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dock8
• Synonyms: 1200017A24Rik; 5830472H07Rik; A130095G14Rik; AI461977
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225172 representing NM_028785
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGCTGCCGAGCGCCGAGCGCCGCGCCTTCGCGCTCAAGATCAACAGGTATTCATCGTCAGAAA
  TAAGGAAGCAGTTTACGCTCCCACCCAACCTCGGACAGTACCATCGGCACAGTATCAGTACATCTGGTTT
  CCCCTCTCTTCAGCTACCTCAGCTTTATGAGCCTGTCGAGCCAGTGGACTTTGAAGGACTCGTGATGACA
  CACTTAAACAGCTTGGATGCAGAGCTGGCCCAGGAGCTGGGGGACCTCACCGATGACGACCTGCATGTGG
  CCTTCACACCCAAAGAATGTAGGACTTTGCAGCACTCTCTGCCAGAGGAAGGAGTTGAACTGGATCCTCA
  CGTCAGAGACTGTGTTCAGACCTATATTCGAGAGTGGCTGATTGTAAACCGGAAAAACCAAGGAAGTTCA
  GAGTTTTGCAGCTTTAAAAAGACGGGATCTCGCAGAGATTTTCAGAAGACGCTTCAGAAACAGACGTTTG
  AGTCAGAAACCTTGGAGTGCAGTGAACCGGACACTCAGACAGGACCCCGTCATCCCTTAAACGTGCTGTG
  TGACGTGTCTGGGAAGGGCCCCCTCACTTCTTGTGACTTCGACCTCCGCAGCCTGCAGCCTGATGAGCGG
  CTGGAAAACCTGCTCCAGCTTGTGAGCGCTGAGGACTTTGAGAAGGAGAAGGAGGAGGCCCGCAAGACCA
  ATCGGCCGGCTGAGCTCTTTGCCCTCTATCCGCCCGTGGATGAGGAGGATGCTGTGGAAATACGTCCAGT
  ACCTGAATGTCCTAAGGAACATCTGGGCAACAGAATATTGGTCAAGGTGCTGACTCTGAAGTTTGAGATT
  GAAATTGAACCTCTGTTTGCCAGTATTGCCCTCTATGACGTTAAAGAAAGGAAAAAGATCTCAGAAAATT
  TCCACTGTGACCTGAACTCCGACCAGTTCAAAGGGTTTCTGCGAGCTCACACACCCTCGATTGACCCATC
  GAGTCAGGCTAGGTCTGCCGTGTTCTCTGTCACCTACCCATCTTCAGACATCTACCTGGTTGTCAAGATT
  GAAAAGGTCCTTCAGCAAGGAGAGATTGCAGACTGTGCAGAACCCTACATGATCATCAAAGAAAGCGATG
  GTGGAAAGAGTAAAGAAAAGGTTGAAAAACTAAAACTTCAAGCTGAATCCTTCTGCCAACGTTTGGGGAA
  ATACCGGATGCCCTTCGCCTGGGCCCCCATTAGCTTAGCAAGCTTCTTCAACATCTCCACCCTTGAAAGG
  GAGAGCACAGATGTGGAGCCTGGGGTTGGGAGGAACTCTGTGGGTGAGAAGAGGAGCTTGTCCCAATCCA
  GGAGGCCCTCTGAGCGAACCCTCTCCTTGGAGGAAAATGGAGTTGGATCCAACTTCAAAGCCACCACCTT
  GGCCACCAACATCTTCTTCAAACAGGAGGGAGATCGCCTTAGTGATGAAGACTTGTTCAAGTTTTTAGCT
  GACTACAAGAGATCTTCATCCCTACAGCGAAGAGTCAAATCCATCCCAGGCTCACTGAGGCTGGAGATAT
  CCCCAGCTCCCGACGTGATGAACTGCTGCCTGACGCCCGAGATGCTGCCAGTCAAACCTTTTCCTGAAAA
  TCGGACGCGTCCACACAAGGAGATTTTGGAATTTCCGATCCGGGAGGTGTACGTCCCTCACACTGTGTAC
  AGAAACCTTCTGTACGTATACCCCCAGCGACTGAACTTCGCTAGCAAGCTAGCATCTGCCCGGAACATCA
  CAATAAAGATTCAGTTTATGTGCGGAGAAGACCCCAGCAATGCTATGCCGGTCATCTTTGGCAAGTCCAG
  TGGGCCTGAATTTCTGCAGGAAGTATATACAGCTATTACATACCATAATAAGTCTCCTGACTTTTACGAA
  GAAGTGAAAATTAAGCTCCCTGCCAAGCTCACAGTGAATCATCACCTCCTCTTCACCTTCTACCACATCA
  GCTGTCAGCAGAAGCAAGGGGCCTCCGGAGAAAGCCTTCTGGGGTACTCGTGGCTGCCGATTCTGTTAAA
  CGAACGTCTTCAAACCGGATCCTACTGTCTGCCTGTTGCCTTGGAAAAACTACCACCCAACTACTCCATA
  CATTCTGCTGAGAAAGTCCCTTTACAGAATCCTCCCATTAAGTGGGCCGAGGGCCATAAGGGGGTATTTA
  ATATTGAAGTGCAAGCTGTTTCTTCCGTCCACACCCAGGATAACCACCTGGAGAAGTTCTTCACCCTTTG
  CCACTCCCTGGAGAGCCAGGTGAGCTTCCCTATCCGTGTGCTGGACCAGAAGATCACCGAGAGCACGCTG
  GAGCACGAGCTGAAACTCAGCATCATCTGCCTCAACTCCTCCCGCCTGGAGCCCCTCGTGCTCTTCCTCC
  ACCTGGTGCTTGACAAGCTGTTCCAGCTTTCCGTGCAGCCCATGGTCATTGCTGGCCAAACAGCAAACTT
  CTCCCAGTTTGCCTTCGAGTCTGTGGTGGCCATTGCCAATAGCCTGCACAACAGCAAGGACCTGAGGAAG
  GACCAGCACGGGAGGAACTGCCTTCTGGCCTCCTACGTGCACTACGTGTTCCGGCTGCCGGAACTGCACA
  GGGATACATCCAAGTCAGGTGGCCCCACCACCGTAGTCCCCGACCCCCGATACCACACATATGGACGCAC
  ATCTGCCGCTGCAGTGAGTTCAAAGCTGATGCAGGCCCGTGTGATGAGCAGCAGCAACCCAGACCTGACT
  GGGTCACACTGTGCAGCCGATGAGGAAGTTAAGAACATCATGTCTTCAAAGATTGCCGATCGCAACTGCA
  GCCGGATGTCTTACTATTGCTCTGGCAATAGTGATGCGCCAGGTTCAACTGCAGCCCCAAGACCAGTCAG
  CAAAAAGCATTTCCATGAGGAGCTTGCCCTGCAGATGGTGGTCAGCACTGGAGTAGTGAGAGAAACGGTC
  TTCAAGTACGCCTGGTTCTTCTTTGAGCTTCTGGTGAAAAGCATGGCACAGTATGTCCATAACCTGGATA
  AACGGGACAGTTTTCGGAGGACTCGTTTTTCTGACCGCTTCAAAGATGACATAACTACCATTGTTAATGT
  GGTCACCTCGGAGATAGCAGCCCTTTTAGTGAAACCTCAGAAGGAAAGCGAGCAGGCAGAAAAGATCAAC
  ATCAGCCTTGCCTTCTTCCTGTATGACCTCCTGTCAATCATGGACAGAGGCTTCGTGTTCAACCTCATCA
  AGCATTACTGCAGCCAGCTGTCAGCCAAGCTGAATATCCTTCCAACGCTCATCTCCATGCGGCTGGAATT
  CCTGAGGATCCTCTGCAGCCATGAGCACTACCTCAACTTGAACCTCCTCTTCATGAATACCGACACCGCA
  CCAGCATCTCCCTGCCCCTCCATATCCTCCCAGAACTCGAGTTCCTGCTCCAGTTTCCAGGACCAAAAGA
  TTGCCAGCATGTTCGATCTGACCCCGGAGTACCGGCAGCAGCACTTCCTTACAGGGCTGCTCTTCACGGA
  GCTGGCTGTTGCCCTGGATGCTGAGGGGGATGGAATTAGCAGAGTACAGAGAAAAGCCGTGAGTGCCATC
  CACAGCCTTCTGTGTTCTCACGACCTGGATCCACGGTGTCGCAAACCGGAAGTGAAAGTCAAAATCGCCG
  CCCTTTACCTGCCGTTGGTCGGCATCATTCTGGACGCTCTGCCACAGCTCTATGACTTTACAGATGCTCG
  CAGTGGAAGGAGTCGTGCCAGTGGCTCGTATGAAGAACAAGATGTGGCCAACGGAATCAACCAGAATGTG
  GCCCTGGCCATAGCGGGGAATCACTTTAATTTGAAGACCAGTGGAGCAATGCTGTCTTCCTTGCCCTATA
  AGCAGTACAACATGCTGAATGCAGACACCACCCGCCACCTCATGATTTGCTTCCTGTGGATCATGAAAAA
  TGCTGATCAGAGCCTCATCAGGAAGTGGATCGCTGACCTGCCTTCCATGCAGCTCAATAGGATTCTGGAC
  CTGCTGTTCATCTGTGTCTCCTGCTTTGAATACAAGGGAAAGCAGAGTTCTGACAAAGTCAGTAACCAGG
  TCCTGCAGAAGTCAAGAGACGTCAAGGCCAAGCTGGAAGAGGCCCTGCTCCGTGGGGAAGGAGCCCGTGG
  GGAGATGATGCGCCGTCGCATTCCAGGGACTGACCGGTTTCCAGGCATAAATGAAAATCTGAGATGGAGG
  AAAGAGCAGACACAGTGGCGGCAGGCTAATGAGAAGCTGGACAAAACAAAGGCAGAGTTAGATCAAGAAG
  CCTTGATCAGTGGCAACCTGGCTACAGAAGCTAATTTGATCATCCTGGATATGCAGGAGAACATCATCCA
  GGCAAGCTCCGCCCTGGACTGTAAAGACAGCCTGCTGGGAGGTGTCCTCCGGGTCCTGGTGAATTCTCTG
  AGCTGTGACCAGAGCACCACCTACCTGACTCACTGTTTCGCAACCCTCCGAGCCCTCATCGCCAAGTTTG
  GAGACCTGCTGTTCGAGGAGGAGATGGAGCAGTGTGCTGACCTGTGTCAGCGGGTGCTACATCACTGCAG
  CAGCAGCATGGACGTCACACGGAGCCAAGCCTGCGCCACCCTCTACCTCCTCATGCGGTTCAGCTTCGGA
  GCCACCAGTAACTTTGCAAGGGTAAAGATGCAAGTGACCATGGCACTGGCATCCCTGGTAGGCAAAGCAC
  CAGACTTCAACGAAGAGCACCTGAGAAGGTCCTTAAGGACAATTTTGGCCTATTCAGAAGAGGACACGGC
  CATGCAGACAACTCCTTTTCCCATGCAGGTGGAGGAACTTCTCTGCAATCTGAACAGCATTCTGTACGAC
  ACAGTGAAGATGAGGGAATTCCAGGAAGACCCTGAGATGCTTATGGACCTCATGTACAGAATTGCCAAGA
  GCTACCAGGCATCGCCTGACCTGCGGCTGACTTGGCTCCAGAACATGGCAGAGAAACACACTAAGAAGAA
  GTGCTTCACAGAGGCCGCCATGTGCCTGGTGCATGCAGCCGCCCTGGTGGCCGAGTACCTGAGCATGCTG
  GAGGACCACAGCTACCTGCCGGTGGGCAGCGTCAGCTTTCAGAATATTTCTTCCAATGTGCTTGAGGAGT
  CTGCAGTCTCTGATGACACCTTGTCACCTGATGAGGACGGCGTATGCTCTGGTCGGTACTTCACTGAGAG
  TGGCCTGGTGGGCCTCCTGGAGCAGGCTGCGGAGCTCTTCAGCACGGGAGGCTTGTACGAGACGGTTAAT
  GAAGTCTACAAGCTGGTCATCCCTATCCTGGAGGCACACAGAGATTTCCGGAAGCTGACCTCCACTCACG
  ACAAGCTGCAGAAGGCCTTCGATAACATCATCAACAAGGACCATAAGAGGATGTTTGGGACCTACTTCCG
  AGTTGGTTTCTACGGATCCCGATTTGGGGATTTGGATGAGCAGGAGTTCGTGTACAAGGAACCCGCAATC
  ACGAAGCTCCCGGAGATCTCACATAGACTAGAGGGATTTTATGGCCAGTGTTTCGGTGCAGAGTTTGTGG
  AAGTGATAAAAGACTCTACTCCAGTGGACAAAACCAAGTTGGATCCTAACAAGGCCTACATTCAGATCAC
  TTTTGTGGAGCCTTACTTTGATGAATATGAGATGAAAGACCGGGTGACCTACTTCGAGAAGAATTTCAAC
  CTCCGGAGGTTCATGTACACCACCCCCTTCACCCTGGAGGGGAGACCCCGGGGCGAGCTTCATGAGCAAC
  ACCGCAGAAACACCGTGCTCACCACCATGCACGCCTTCCCCTACATCAAGACCAGGATCCGAGTCAGCCA
  GAAAGAGGAGTTCGTTTTGACTCCGATTGAGGTTGCCATTGAAGATATGAAGAAGAAGACCCTGCAGTTA
  GCCGTGGCCACTCACCAGGAGCCCCCTGATGCAAAGATGCTGCAAATGGTACTGCAGGGCTCTGTAGGAG
  CCACTGTAAATCAGGGACCACTGGAGGTGGCCCAAGTGTTCTTGGCTGAAATTCCAGCTGACCCAAAGCT
  CTACCGACATCACAACAAGCTGAGGTTGTGCTTCAAGGAGTTCATAATGCGATGCGGAGAGGCCGTGGAG
  AAGAACAGGCGACTCATCACCGCAGAGCAGCGGGAGTACCAGCAGGAGCTGAAGAAGAACTACAACAAGC
  TGAGAGACAGCCTCAGGCCCATGATTGAGCGGAAAATCCCAGAGCTCTACAAGCCCATATTCAGAGTTGA
  CAGTCAGAAGAGGGACTCTTTCCACAGATCTAGTTTCAGGAAATGTGAAACCCAGTTGTCACAGGGCAGC
  TGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_028785
• Insert Size: 6303 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028785.3, NP_083061.2
• RefSeq Size: 7810 bp
• RefSeq ORF: 6303 bp
• Locus ID: 76088
• UniProt ID: Q8C147
• Cytogenetics: 19 B
• Gene Summary: Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed:28028151, PubMed:22461490). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (PubMed:22461490, PubMed:25713392). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed:28028151). Is involved in NK cell cytotoxicity controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (By similarity).[UniProtKB/Swiss-Prot Function]