Ckap5 (NM_001165989) Mouse Untagged Clone

CAT#: MC225139

Ckap5 (untagged) - Mouse cytoskeleton associated protein 5 (Ckap5), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ckap5
• Synonyms: 3110043H24Rik; 4930432B04Rik; D730027C18Rik; mKIAA0097; T25636
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225139 representing NM_001165989
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGATGACAGTGAGTGGTTGAAATTACCAGTTGATCAGAAATGTGAACACAAGCTATGGAAAGCAA
  GATTAAGTGGGTATGAAGAAGCCTTGAAGATCTTCCAGAAAATAAAGGATGAAAAGAGTCCAGAATGGTC
  CAAATACTTAGGATTAATCAAAAAATTTGTCACTGATTCCAATGCAGTGGTTCAATTGAAAGGACTAGAA
  GCTGCACTTGTCTATGTTGAAAATGCTCATGTTGCAGGAAAAACAACGGGAGAGGTTGTATCAGGTGTTG
  TAAGTAAAGTCTTCAACCAACCTAAAGCCAAAGCCAAGGAACTGGGCATAGAGATTTGCCTCATGTATGT
  TGAGATTGAAAAAGGAGAGTCTGTACAAGAAGAGCTTCTGAAGGGCCTGGATAACAAGAACCCCAAGATC
  ATAGTGGCCTGTATAGAAACACTGAGGAAAGCCTTAAGTGAATTTGGTTCCAAAATCATCTCACTTAAGC
  CAATTATTAAAGTGTTGCCAAAACTATTTGAATCTCGAGACAAGGCTGTTCGGGATGAAGCCAAATTATT
  TGCTATAGAGATTTATCGATGGAATCGAGATGCTGTGAAACACACACTACAAAATATAAATTCTGTCCAG
  TTGAAAGAATTAGAAGAAGAGTGGGTCAAGCTGCCAACAGGTGCTCCTAAGCCCAGTCGGTTTCTGCGTT
  CCCAACAAGAACTAGAAGCTAAACTGGAACAGCAACAGTCTGCTGGTGGAGATGCAGAAGGGGGTGGTGA
  TGATGGTGATGAGGTACCACAGGTAGATGCTTATGAGCTTCTGGATGCGGTAGAGATCCTTTCCAAACTT
  CCCAAAGACTTTTATGACAAAATTGAGGCAAAGAAATGGCAAGAGAGAAAAGAAGCTCTGGAGGCTGTAG
  AAGTACTAGTGAAAAACCCCAAGCTGGAAGCAGGCGATTACGCAGATTTAGTGAAAGCATTAAAAAAGGT
  TGTTGGAAAGGACACCAATGTCATGTTGGTAGCTTTGGCAGCAAAATGTCTTACTGGCTTAGCTGTTGGG
  CTCAGAAAGAAATTTGGACAATATGCAGGACATGTTGTGCCAACAATTTTGGAAAAATTCAAAGAAAAGA
  AGCCTCAAGTGGTACAAGCCCTGCAGGAGGCAATTGATGCAATCTTCCTCACTACCACACTACAGAACAT
  CAGTGAGGATGTCTTAGCAGTAATGGATAATAAAAATCCCACCATCAAGCAGCAGACATCACTTTTTATT
  GCAAGAAGCTTTCGTCACTGCACTTCCTCTACATTGCCGAAGAGTCTACTAAAACCCTTTTGTGCTGCTC
  TACTTAAGCACATCAATGATTCTGCTCCTGAAGTCAGAGATGCTGCATTTGAAGCACTGGGTACAGCTTT
  GAAGGTGGTTGGTGAGAAATCAGTAAACCCATTTTTAGCTGATGTTGACAAACTCAAACTTGATAGGATC
  AAAGAATGTTCAGAAAAAGTAGAACTGGTTCATGGTAAAAAATCTGGACTTGCAACTGAAAAGAAGGAAT
  CCAAGCCTCTACCTGGAAGGGCTGCAGCATCAGGGGCTGCAGGGGACAAAGACACAAAAGATGTTTCAGG
  ACCCAAACCAGGACCGTTAAAAAAAACACCTACTGCTAAGGCTGGTGGACCATCTAAGAAAGGTAAAACA
  ACTGCACCAGGTGGCTCAGCAAGTGCCGGGACAAAGAATAAGAAAGGATTGGAGACCAAAGAAATAGTGG
  AGCCAGAACTCTCAATTGAAGTATGTGAAGAGAAAGCTTCAGCTGTTCTTCCCCCAACCTGTATTCAGCT
  TCTAGACAGCAGCAACTGGAAGGAAAGGCTGGCCTGTATGGAAGAATTCCAAAAGGCTGTTGAACTAATG
  GAGCGAACTGAGATGCCGTGCCAGGCATTGGTGAAGATGCTAGCAAAAAAACCTGGATGGAAAGAAACTA
  ATTTTCAGGTGATGCAGATGAAGCTTCACATTGTTGCTTTGATTGCCCAGAAGGGAAATTTTTCTAAAAC
  GTCTGCTCAGATTGTATTAGATGGCCTTGTAGACAAGATTGGAGATGTGAAATGTGGGAACAATGCAAAA
  GAAGCCATGACAGCAATAGCTGAAGCGTGCATGCTGCCCTGGACAGCTGAGCAGGTGATGTCAATGGCCT
  TCTCACAAAAGAATCCTAAGAATCAATCAGAAACTCTAAATTGGCTATCAAATGCAATAAAAGAATTTGG
  ATTTTCTGAGTTGAATGTCAAAGCTTTCATTAGCAACGTGAAGACTGCTCTTGCTGCAACAAATCCTGCT
  GTGAGGACTTCTGCCATCACCCTGCTTGGCGTGATGTATCTATATGTTGGTCCTTCTTTGAGAATGATCT
  TTGAGGATGAGAAGCCTGCCCTCCTATCCCAGATAGATGCAGAATTTCAAAAGATGCAGGGACAAAGTCC
  TCCCGCTCCAACGAGGGGAATTGCTAAGCACAGCACAAGTGCTACAGATGAAGGAGAAGATGGAGAGGAA
  CCAGGTGAAGGGGGCAATGATGTTGTTGATCTTTTGCCGAGGATTGAGATCAGTGACAAAATTACTTCAG
  AGTTGGTGTCTAAGATTGGTGACAAGAATTGGAAGATCAGGAAAGAAGGCCTAGATGAAGTGGCAGGTAT
  TATCAATGAAGCAAAATTTATCCAACCAAATATAGGTGAACTTCCAACTGCCTTGAAGGGTCGACTGAAT
  GATTCAAACAAAATCTTGGTGCAGCAGACACTGAATATCCTTCAGCAACTGGCAGTAGCCATGGGTGCAA
  ACATCAGGCAGCATGTAAAGAACCTGGGCATCCCTGTCATTACTGTTCTCGGAGATAGCAAGAACAATGT
  TCGAGCTGCTGCCCTAGCAACTGTGAATGCTTGGGCAGAACAAACTGGCATGAAAGAATGGCTGGAGGGA
  GAAGATCTTTCTGAAGAACTTAAAAAGGAAAATCCCTTCCTGAGGCAAGAGCTTCTGGGATGGCTGGCAG
  AGAAACTACCTACACTCCGTTCCACTCCTACAGATCTTATCCTTTGTGTTCCTCATCTGTATTCCTGTCT
  AGAAGATCGAAATGGAGATGTACGGAAGAAGGCCCAAGATGCTTTGCCATTCTTTATGATGCATTTAGGA
  TATGAAAAAATGGCTAAGGCTACTGGGAAACTAAAGCCAACTTCTAAAGATCAAGTACTGGCCATGCTAG
  AAAAGGCCAAAGCTAACATGCCATCCAAGCCTGCTGCACCTGCTAAGGCCATGTCCAAACCAATGGGAGG
  GTCGGCTCCAGCCAAAACCCAGCCTATACCAGCACCTGTTGAAGATTCTGTTTCCAGTACCATAGAAGCT
  AAGCCTGATCTGAAAAAGGCTAAAGCTCCAGGCGTTTCTTCCAAAGCAAAGAGTGTACAAGGGAAGAAAG
  TGCCAAGCAAAACCACCTTAAAGGAAGACGACGACAAATCTGGTCCTATTTTTATTGTTGTTCCAAATGG
  AAAGGAGCAAAGGATGAGAGATGAGAAAGGATTAAAGGTGCTAAAGTGGAATTTTACTACCCCACGGGAT
  GAGTACATTGAACAGCTGAAGACTCAGATGTCTACCTGTGTGGCTAAATGGTTACAGGATGAAATGTTTC
  ACTCAGATTTCCAGCATCACAATAAAGCCCTAGCTGTCATGGTTGATCACTTGGAAAGTGAAAAAGACGG
  TGTCATCAGTTGCTTAGATCTTATCTTAAAATGGCTTACCCTCCGGTTTTTCGACACCAATACAAGCGTT
  CTAATGAAAGCACTGGAATATTTAAAATTGCTTTTCACCCTGCTGAGTGAGGAAGAGTATCATCTTACTG
  AGAATGAAGCATCGTCCTTTATCCCCTATCTCATCCTCAAGGTTGGAGAACCAAAGGATGTCATTCGTAA
  AGATGTCCGTGCTATCCTGAACCGGATGTGCCTTGTCTACCCAGCCAGCAAAATGTTCCCCTTCATTATG
  GAAGGAACCAAGTCCAAAAACTCTAAGCAGAGAGCAGAGTGCCTGGAAGAGCTAGGTTGCTTAATTGAGT
  CCTATGGTATGAATGTTTGTCAGCCAACCCCAGGAAAAGCCTTAAAGGAGATAGCTATTCACATAGGAGA
  CCGAGACAATGCTGTACGGAATGCTGCCCTCAACACCATTGTAACAGTGTACAACGTGCATGGAGATCAG
  GTGTTCAAACTGATTGGAAATCTTTCTGAGAAAGACATGAGCATGCTTGAGGAGAGGATAAAGCGGTCAG
  CAAAGAGACCCTCTGCTGCACCTGTGAAACAGGCCGAAGAGAAACCTCAGCGCACACAGAATATAAACTC
  TAATGCCAACATGCTACGCAAGGGACCAGCTGAGGATATGTCCTCCAAACTTAATCAAGCTCGAAGCCTG
  AGTGGGCATCCTGAAGCAGCCCAGATGGTTCGCCGAGAATTCCAGCTGGATTTAGATGAGATTGAAAATG
  ATAATGGTACAGTCCGCTGTGAAATGCCAGAGCTTGTCCAGCACAAACTTGATGACATTTTTGAACCTGT
  CCTTATTCCTGAACCTAAGATCCGGGCTGTTTCTCCACATTTTGATGACATGCACAGTAATACAGCATCC
  ACAATCAATTTCATTATCTCCCAAGTAGCCAGTGGTGACATCAACACAAGCATCCAAGCTCTGACACAGA
  TTGATGAGGTCCTGAGGCAGGAAGACAAAGCTGAAGCTATGTCTGGCCACATTGATCAGTTTCTGATAGC
  AACATTCATGCAGCTAAGACTCATCTACAGCACACACATGGCAGATGAGAAGTTGGACAAGGATGAAATC
  ATCAAGTTGTATAGCTGTATCATTGGCAACATGATTTCGCTATTTCAGATAGAGAGCTTGGCCCGGGAGG
  CCTCCACTGGAGTACTGAAAGACCTGATGCATGGACTGATCACCTTAATGCTGGATTCTCGGATTGAAGA
  TCTGGAGGAAGGACAACAGGTGATTCGTTCTGTGAACCTCTTGGTGGTGAAGGTTTTGGAGAAATCAGAC
  CAGACCAATATCCTCAGTGCCCTGCTTGTTTTACTTCAGGACAGCCTGCTAGCAACAGCTAGTTCTCCCA
  AATTCTCAGAGCTTGTTATGAAGTGTCTATGGAGAATGGTTCGACTATTACCTGACACCATCAACAGCAT
  TAACCTAGACAGAATTCTTCTGGACATCCACATTTTCATGAAGGTCTTTCCCAAAGAGAAATTGAAGCAA
  TGCAAAAGTGAATTTCCTATAAGGACTCTAAAGACCCTTCTACATACTTTATGCAAATTAAAAGGACCTA
  AAATCCTAGACCACCTTACAATGATTGATAATAAGAATGAGTCAGAATTGGAGGCCCACCTGTGTCGGAT
  GATGAAGCATAGTATGGACCAGACTGGGAGCAAGTCTGATAAGGAAACAGAAAAGGGAGCTTCTCGAATA
  GATGAAAAATCATCAAAGGCCAAAGTAAATGATTTCTTAGCTGAGATTTTTAAGAAGATTGGCTCCAAAG
  AGAACACTAAGGAGGGCCTAGCAGAGTTATATGAATATAAGAAGAAATATTCAGATACTGACATTGAACC
  ATTTCTGAAAAATTCCTCGCAGTTCTTTCAGAGTTATGTTGAGAGAGGCCTTCGGGTGATTGAGATGGAG
  AGAGAAAGCAAAGGCCGGATTCCTACTTCAACAGGCATTTCCCCTCAGATGGAGGTCACATGTGTGCCCA
  CCCCCACAAGCACAGTGTCCTCCCTAGGTAACACAAATGGAGAAGAAGTGGGGCCATCTGTCTACTTGGA
  AAGGCTAAAAATTCTGCGGCAGCGGTGTGGTCTTGACAACACAAAGCAAGATGACCGGCCTCCACTGACC
  TCTTTGCTCTCCAAACCAGCAATTCCTCCAGTTGCCTCCTCCACAGACATGCTCCATAGCAAGCTCTCAC
  AGCTCCGGGAGTCCCGGGAACAGCACCAGCACTCAGACCTGGATTCCAACCAGACCCACTCTGCAGGAAC
  CATGACCTCCTCCTCCTCCACAACTAACATTGATGACTTGAAAAAGAGACTGGAGAGAATAAAGAGCAGT
  CGCAAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001165989
• Insert Size: 6099 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001165989.1, NP_001159461.1
• RefSeq Size: 6584 bp
• RefSeq ORF: 6099 bp
• Locus ID: 75786
• Cytogenetics: 2 E1
• Gene Summary: Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.