Fancm (NM_178912) Mouse Untagged Clone

CAT#: MC225128

Fancm (untagged) - Mouse Fanconi anemia, complementation group M (Fancm), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fancm
• Synonyms: AI427100; C730036B14Rik; D12Ertd364e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225128 representing NM_178912
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGACGGCAGAGAACGCTCTTCCAGACGTGGGGCCCGAGCCTCGTGCGGGGCTCCGGAGACTCGG
  GATGCGGCCAGCCGCGCTCGCCCGCGATGGCGGAGGCGCTGCCGGAGGAGGACGACGAGGTGCTGCTGGT
  GGCGGCGTACGAGGCTGAGCGGCAGCTGGATCCCGGGGATGGCGGCTTCTGCGCGGCTGCGGGAGCCCTG
  TGGATCTACCCCACCAATTGCCCCGTGCGCGACTACCAGCTGGACATCTCCCGGAGCGCGCTGTTCTGCA
  ACACCCTGGTGTGCCTGCCCACCGGGCTGGGGAAGACCTTCATTGCCGCCGTGGTCATGTATAATTTCTA
  CCGCTGGTTCCCTTCTGGCAAGGTGGTTTTCATGGCACCCACGAAGCCCCTGGTAACACAGCAGATGGAA
  GCCTGCTTCCACGTGATGGGTATTCCACAGTCTCACATGGCTGAAATGACAGGTTCAACTCAAGCTGTTA
  ACAGGAAGGAGATATGGTCCAGCAGGAGGGTTCTTTTCCTGACACCGCAGGTCATGGTAAATGACCTTAC
  TAGAGGGGCTGTTCCTGCCACCCACGTAAAGTGTCTTGTGGTGGATGAAGCTCATAAGGCACTTGGGAAC
  TATGCTTACTGCCAGGTGGTAAGAGAATTGGTCAAATACACCACTCACTTTCGTATCTTGGCTCTTAGTG
  CCACGCCAGGTAGTGATATCAAGGCTGTGCAACAGGTGATCACTAACCTACTAATTGGGAAGATAGAGCT
  TCGTTCGGAAGAGTCTCCCGATATTTTGCCTTACTCTCATGAAAGAAGAGTTGAGAAGCTCGTTGTCCCC
  CTTGGGGAAGAACTAGGAGCCATTCAAAAGACGTATATCCAGATTTTGGAAACATTTGCCAGTTCTTTGA
  TTCATAGAAATGTTTTGATGAAAAGAGACATTCCCAATCTAACAAAATACCAGATAATTCTGGCAAGAGA
  TCAATTTAGGAAAAACCCATCCCCAAATATTGTGGGAATTCAGCAAGGCATAATTGAAGGGGAGTTTGCC
  CTCTGCATTAGTTTATATCATGGTTATGAGTTACTGCAGCAAATGGGAATGCGGTCATTGTATTTCTTTC
  TTTCTGGAATCATGGATGGAACTAAAGGAATGACACGGGCAAGAAATGAGCTCAGCCGAAATGAGGACTT
  CATGAAGCTGTACACTCACCTGCAGAGCGCGTTTGCACCAGCCAGCACCAGCGATGCTTCGGCTTTCCAG
  AGAGGAAATAAAGAGAAAAAATTTGTCTATAGTCATCCAAAGTTAAAGAAACTGGAAGAAGTTATACTGG
  AACACTTCAAGTCATGGAATGCTAAAGCCACCACTGAGAAGAAGTGCCACGAGAGCAGAGTCATGATCTT
  CTCGTCATTCCGAGATAGCGTTGAGGAGATTGCAGAAATGCTTTTACAGCACCGGCCAGTTATTAGAGTG
  ATGACCTTTGTTGGCCATGCTTCAGGGAAAAACACAAAAGGTTTTACCCAGAAGGAACAACTGCAGGTGG
  TGAGACAGTTCCGAGACGGTGGCTATAACACACTGGTTTCCACCTGTGTTGGAGAAGAAGGTTTGGATAT
  AGGGGAAGTGGATCTTATAATATGCTTTGATGCCCAGAAGAGCCCAATCCGCCTCATCCAGCGCATGGGT
  AGAACTGGCCGCAAACGGCAAGGCAGGATTGTCGTTATCCTCGCTGAAGGACGGGAAGAGCGTACATATA
  ATCAGAGTCAGTCCAACAAAAAAAATATTTATAAAGCCATTTCAGGTAACAGACAAGTGCTGCGTTTATA
  CCAAGGAAGTCCACGAATGGTTCCTGATAAAATCAATCCTGAATTACACAAAATGTATATCACACATGGT
  GTCTATGAACCAGAGAAGGCTCGGAGTGTACAGCGAAGGCCGTTTTCCTCAAGGGGTGGAATAAAAGCTA
  GTAAATCGAATAAAGATGGGCTGTTATCAGAAGAGGAATTTAACCTATGGAGCAGACTCTATAGGTTAGG
  AGACAGTGACCAGGTTAAAGGAGTGGCATTGCCTCAGTCTCACTTCCCATCTTTACAGGAGGACAGAGTG
  ATTCAAGACCCAACTACTAGAATTCACCAACTCTCTCTCTCTGAATGGAGTCTCTGGCAAGATCGTCCGC
  TTCCTACACATCAAGTTGACCATTCAGATCGTTGCCACCATTTTATAAGCATTATGAAAATGATAGAAGG
  AATGAGACACGAAGAGGGTGAATGCAGCTATGAGTTGAAAATTAGACCTTTTCTACAAATGGAAGATGTC
  TGCTCAAAATATCACGCTCCTAGGAATGGATATAATAATGTAGCCAGTGTCGCCAGTAGTGCTCACCAGA
  AGTCTTCATTTAGACCGAGTGTAGATGCAGGAGGCTCCTTGACGGTGATAGAGTCTGAGGAAGAGCATGC
  TGACACTGTTAAACAAAGGGATTCTAAATGGACCAAGATTACTTCTCTAAGGGAAAAACCCTGTAGAGCA
  GGAAGAAAAGGCCAGACCTGTGAGCACAGTGAGGGTGAGGGTGAGGATGGGGATGCAGGTTCTTCCGATG
  CTGATGGACAGTCTCCTGCCGAAGCTGATTCCCAGGTAGACCCACCCAGTGGTGAAAGGATGGCAGATGT
  AGGTGGGATCTCCATCTTGGGTGCTGTCACTGAAGAGGATAACCATCCAGGAACATTACAAATGGAGTGT
  CAGGTCACAAATAAATCATGTGCCAGGTACTCATTAGATTCTGGTTATAGTAGTTTCAGTGATGAGAAAT
  CTGTATCATCAAATTTGTTTCTTCCATTGGAAGAAGAGCTGTTCACCGATCGAGCTGCTGAGCAGTTTTA
  TAATTGTCGGCCCATGACAGAAGACGTACTAGCTAATGTGGAGAGATTTTTATCCCGTTCTCCCCCGTCT
  CTCAGTGGACTCTCAGACTTGGTATATGATGTTACTCAGGGTTGTGAATTTGATAATGCATCCTGTTCAC
  CCTACCCAGAGCATGAACACAGTCCTAGACCTGTTTCTCCAGCGTCACACTCTGCTGGGAATTCTCAACA
  AAACTTAGAATCGAATTCAGCTAAACGTATTAGTCATCCTACTGAAAAATACTGTCTCCCTGGCACCACT
  CATAATAAGGTTTCTGATCGGCCCTCCTTCTGTGAGTCTGATAGCGAAGGCCATAATATTAAATATCAAA
  ATTCAGGATCTAACAGTTGTGCTCAAATACAAGCAGACCTTGAAAATAACTTCGTTGACAAGAACAGCCA
  TGATGATAGTGAACCCCCAGTATTGTTCACTGATGAAGATGAAAGCTTACTATTATTTGAAGATGATTTT
  AAGAATATTGAAGATGGTCCTGAAGAGTTAAATGGGGCGAGCCTTCCTCCCTTCAACAGTATCAGTCAGC
  CCCTCCGTGTGTCAGGTAAAACTCTCACAAGTGAAATGCCTCCAGTCTCTCACTTCTTAATTTCTGATGA
  ACTGTTGTTAGATGATGATTCTGAACCTGAAGACCAAATCGTCTGTGGTGCCAAGAGTTGGAAATGTCAG
  GAAGGGGTGGAGGATGGACAAGAAGAGCTGAGGACTGACGGACAGACTTTTGATTGCTCTGTAGACTTAT
  TTTCTGTTACCTTTGACTTAGGATTTCGTTGTAGTTCGGGTTCAGATGATGAAATGCTAGCAGGTGCATC
  TGATAGGACTCGCACCCTGGGAGCGGCCGATGTGTCTGGAAGGCATTCTGATAAGGAGATAAAGGATGCC
  GGTGGCGCTTCAGGTCCACTGGGTAGAGCCATATCACCAATCCCAACTGAGACAGCGCAGTGGTCGCCTT
  GGGCACAGAACAAGGAATATGCTTCTTTCCACGTTGCCTCATCCTCGCCAGTGAAGCAGAGAGTCAGGAG
  CACACCGCTGTCTAAGTCACACGCGAGCTCCAAGACAGGAGCACACATGCTCAAGACACTAGACTCTACC
  AAGGAGAAAGCAGGTGGACAGGGCTTCAAAATGGCATTGAACCCAAGACTGGGCCATCTAGGATTTTCTG
  TAGAAGAGACCAAGAGCAGCGATCAGGTCTTTGTGCATCAGTCCCCTCGGCGCACTGAAGTTGAACACTT
  AACTAGTGAAAGCGAAGATGATGTTTTCCTGAGAAAAACTAAAAAACCAAAAAGAAATGTCCTAAAGTCC
  CCGGAGGATCAGAAAAACAACGAAGTTGACTCACCCATTCATGCTGTAAAAAAACCCAGAGTTCTCAGGT
  CAGAATTAGCATCTAGCGATGATGAGAGTGAGAATTTTGGCAGAACGTGTCCAAGATTGGAACACTTCAA
  GGGTCGTAACAGGAACATCAGAAAAGGCTCCGCAGCCCAAAAGAACCGGAGTCAAGTAAAGACTATGGCT
  CGGAGGTTTTTAGACGACGAGGCAGAGGTTTCTGGAGAGGATGTGGACTGTGTCTCAGCAGATGAGGAGG
  ATGAGTCTGAGAACGAGCAGGATTCCTCCCTGCTCGACTTTGTAAATGACCGGACCCAGCTTTCACAGGC
  TATAAATGATTCTGAAATGAGAGCTATTTACATGAAGTCTGTACGCAGTCCATTGATGAGCACTAAGTAC
  AGAATGGTCCGTGAGAAGCGTCCCAACATGAACATTTTCTCACAGATTCCTGAGCAAGATGAAAACTATT
  TAGAGGACAGTTTTTGTGTTGATGAAGAAGAGTCTTGCAAAAGCCAATCAAGTGAAGAAGAAATTTCTGT
  TGATTTTAACTTAACTAAAGATTCCTTTACAGATGAGGACATACGATATAAAACTCGGTATGCAGTAAAG
  ATCAAACAAATGAACAAGAAACAAAACTACACACGGCCAAGAAAGAAATTGTCTAGAATTATCTTACCCG
  ACGATGACTCTAGTGAGGAAGAAAACATCCCGAAGGACAGAGAACACTCCGTTGCTGGTGGCCACGCTGC
  TGCTGAGCACACACAGCAGGGCCAGCTCTGGGCTTCAGGGCCATCCGGGTCCTCAGTGCCTCCCCAGGTC
  CTTTCTGATCCATCGTGGAATCAGTCATCCAGGCAGAGACTACAGGTGCAACCCAGTATAACAGATGCCG
  TGCCTAGAACCTTGAACGTGAAGGCTCAGAGCCATAATAAAATCAAATCTGCTTCACCACCATGTACTGG
  TGTTGAATCAAGGAAAGAATATGGGAACCATCCAGTTCAGTTGAAGGCTGACAGTCAGGAGCACAGTGAC
  ACCTCAGCAGCACCCTGTTCTACTTCACTCCTGCATGTGGCTGAGGGACATACAGCCCCTAGACATCTGC
  AAGAAGGAAACAGGGCTTGCATTCTTGTAGACAGTCGTGAAATCACTACCGGTTTGGAAGTAATCTCTTC
  GCTAAGAACAGTTCATGGGTTACAAGTAGAGATTTGTCCTCTTAATGGCTGTGACTACATCGTAAGCAGC
  CGAATGGTCGTAGTAAGGAGGTCTCAGTCTGAAATGTTAAGTAATACCAGCAAGAACAAGTTCATCGAGC
  AGATGCAGCGCCTGCAGAGCATGTTTCAAAGGATATGTGTGATTGTGGAAAAAGACAGAGAAAAAGCAGG
  AGACACATCAAAAAAATTCAGAAGAACGAAGTGCTATGACAGTTTGCTGACTGCCTTAGTTGGCGCCGGA
  ATCCGAATCCTTTTCAGCTCTGGACAAGAAGAAACTGCAGATTTACTAAAGGAGCTGTCATTGGTGGAAC
  AAAGAAAGAATGCTGGTATTCATATCCCAGCAGTGCTAAACACTAGTAAACTTGAAGCACTTCCATTCTA
  TTTAAGTATTCCTGGTATAAGTTACATCACAGCCTTAAATATGTGTCACCAGTTTTCATCTGTGAAAAAG
  ATGGCTAACAGCTCCCCTGAAGAAATCTCCACATGTGCGCAAGTAAATCATCAAAAGGCTGAAGAGATCT
  ATAAATATATTCACTATATATTTGACATGCAAATGTTACCAAATGATCTTAACCAGGAAAGACAAAAACC
  TGATACATGCTTAACACTTGGAGTCGCTATGAAAGAACTCTCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178912
• Insert Size: 6066 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178912.3, NP_849243.2
• RefSeq Size: 7775 bp
• RefSeq ORF: 6066 bp
• Locus ID: 104806
• UniProt ID: Q8BGE5
• Cytogenetics: 12 27.21 cM
• Gene Summary: DNA-dependent ATPase component of the Fanconi anemia (FA) core complex. Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage. In complex with CENPS and CENPX, binds double-stranded DNA (dsDNA), fork-structured DNA (fsDNA) and Holliday junction substrates. Its ATP-dependent DNA branch migration activity can process branched DNA structures such as a movable replication fork. This activity is strongly stimulated in the presence of CENPS and CENPX. In complex with FAAP24, efficiently binds to single-strand DNA (ssDNA), splayed-arm DNA, and 3'-flap substrates. In vitro, on its own, strongly binds ssDNA oligomers and weakly fsDNA, but does not bind to dsDNA.[UniProtKB/Swiss-Prot Function]