Myh6 (NM_010856) Mouse Untagged Clone

CAT#: MC225064

Myh6 (untagged) - Mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myh6
• Synonyms: A830009F23Rik; AA517445; alpha-MHC; Myhc-a; Myhca
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC225064 representing NM_010856
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGACGCCCAGATGGCTGACTTCGGGGCAGCAGCCCAGTACCTCCGAAAGTCAGAGAAGGAACGCC
  TAGAGGCCCAGACCCGGCCCTTTGACATCCGCACGGAGTGCTTCGTGCCTGATGACAAGGAGGAGTATGT
  TAAGGCCAAGGTCGTGTCCCGGGAAGGGGGCAAAGTCACTGCGGAAACTGAAAACGGAAAGACGGTGACC
  ATAAAGGAGGACCAGGTGATGCAGCAGAACCCACCCAAGTTCGACAAGATCGAGGACATGGCCATGCTGA
  CCTTCCTGCACGAGCCGGCTGTGCTGTACAACCTCAAGGAGCGCTACGCGGCCTGGATGATCTATACCTA
  CTCAGGCCTCTTCTGCGTCACCGTCAACCCCTATAAGTGGCTGCCTGTGTACAATGCGGAAGTGGTGGCC
  GCCTACCGGGGCAAGAAGAGGAGCGAGGCCCCTCCTCACATCTTCTCCATCTCTGACAACGCCTATCAGT
  ACATGCTGACAGATCGGGAGAATCAGTCCATCCTCATCACCGGAGAATCCGGAGCGGGGAAGACTGTGAA
  CACAAAACGTGTCATCCAGTACTTTGCCAGCATTGCAGCCATAGGGGACCGTAGCAAGAAGGAAAATCCT
  AATGCAAACAAGGGCACCCTGGAGGACCAGATTATCCAGGCTAACCCCGCTCTGGAGGCCTTCGGCAACG
  CCAAGACTGTCCGGAATGACAACTCCTCCCGCTTTGGGAAATTCATCAGGATCCACTTTGGAGCTACTGG
  AAAGCTGGCTTCTGCAGACATAGAGACCTACCTTCTGGAGAAGTCCCGGGTGATCTTCCAGCTAAAGGCT
  GAGAGGAACTACCACATCTTCTACCAGATCCTGTCCAACAAGAAGCCGGAGCTGCTGGACATGCTGCTGG
  TCACCAACAACCCATACGACTACGCCTTCGTCTCTCAGGGAGAGGTGTCCGTGGCCTCCATTGATGACTC
  TGAGGAGCTCTTGGCCACTGATAGTGCCTTTGATGTGCTGAGCTTCACGGCAGAGGAGAAGGCTGGTGTC
  TACAAGCTGACAGGGGCCATCATGCACTACGGAAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGG
  CGGAGCCTGATGGCACAGAAGATGCTGACAAATCTGCCTACCTTATGGGGCTGAACTCAGCTGACCTGCT
  CAAGGGCCTGTGTCACCCTCGGGTGAAGGTGGGGAACGAGTATGTCACCAAGGGGCAGAGTGTACAGCAA
  GTGTACTATTCCATCGGGGCACTGGCCAAGTCAGTGTACGAGAAGATGTTCAACTGGATGGTGACACGCA
  TCAACGCAACCCTGGAGACCAAGCAGCCGCGCCAGTACTTCATAGGTGTCCTGGACATTGCCGGCTTTGA
  GATCTTCGATTTCAACAGCTTTGAGCAGCTGTGCATCAACTTCACCAATGAGAAGCTGCAGCAGTTCTTC
  AACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAGGGCATTGAGTGGGAGTTTATCGACT
  TCGGCATGGACCTGCAGGCCTGCATCGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTCGAGGA
  GGAGTGCATGTTCCCCAAGGCCTCAGACATGACCTTCAAGGCCAAGCTGTATGACAACCACCTGGGCAAA
  TCCAACAACTTCCAGAAGCCTCGCAATGTCAAGGGGAAGCAGGAAGCCCACTTCTCCTTGGTCCACTATG
  CTGGCACCGTGGACTACAACATTATGGGCTGGCTGGAAAAGAACAAGGACCCACTCAATGAGACGGTGGT
  GGGTTTGTACCAGAAGTCCTCCCTCAAGCTCATGGCTACACTCTTCTCTACCTATGCTTCTGCTGATACC
  GGTGACAGTGGTAAAGGCAAAGGAGGCAAGAAGAAAGGCTCATCCTTCCAAACAGTGTCTGCTCTCCACC
  GGGAAAATCTGAACAAGCTGATGACAAACCTGAAGACCACCCACCCTCACTTTGTGCGCTGCATCATTCC
  CAACGAGCGAAAGGCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGATGCAATGGCGTG
  CTGGAGGGTATCCGCATCTGCAGGAAGGGCTTCCCCAACCGCATTCTCTATGGGGACTTCCGGCAGAGGT
  ATCGCATCCTGAACCCAGCAGCCATCCCTGAGGGGCAATTCATTGATAGCAGGAAAGGGGCTGAGAAACT
  GCTGGGCTCCCTGGACATTGACCACAACCAATACAAGTTTGGCCACACCAAGGTGTTCTTCAAGGCGGGC
  CTGCTGGGGCTGCTCGAGGAGATGCGAGATGAGAGGCTGAGCCGTATCATCACCAGAATCCAGGCCCAGG
  CCCGAGGGCAGCTCATGCGCATTGAGTTCAAGAAGATAGTGGAACGCAGGGATGCCCTGCTGGTTATCCA
  GTGGAACATTCGGGCCTTCATGGGGGTCAAGAATTGGCCATGGATGAAGCTCTACTTCAAGATCAAACCG
  CTGCTGAAGAGCGCAGAGACGGAGAAGGAGATGGCCAACATGAAGGAGGAGTTTGGGCGAGTCAAAGATG
  CACTGGAGAAGTCTGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTGCTGCAGGAGAAGAA
  TGACCTACAGCTCCAAGTGCAGGCGGAACAAGACAACCTCAATGATGCAGAGGAGCGCTGTGACCAGCTG
  ATCAAGAACAAGATCCAGCTGGAGGCCAAGGTGAAGGAGATGACCGAGAGGCTGGAGGACGAGGAGGAGA
  TGAACGCCGAGCTCACTGCCAAGAAGCGCAAGCTGGAAGATGAGTGCTCAGAGCTCAAGAAGGATATTGA
  TGACCTGGAGCTGACGCTGGCCAAGGTGGAAAAGGAAAAGCATGCAACAGAGAACAAGGTTAAAAACCTA
  ACAGAGGAGATGGCTGGGCTGGATGAAATCATTGCCAAGCTGACCAAAGAGAAGAAAGCTCTGCAAGAAG
  CCCACCAGCAAGCCCTCGATGACCTGCAGGCTGAAGAAGACAAGGTCAACACGCTGACCAAGTCCAAAGT
  CAAGCTGGAGCAGCAGGTGGATGATCTGGAGGGATCCCTGGAGCAGGAGAAGAAAGTGCGCATGGACCTA
  GAGCGAGCCAAGCGGAAGCTGGAGGGAGACCTGAAGCTGACCCAGGAGAGCATCATGGACCTGGAGAATG
  ACAAGCTTCAGCTGGAAGAAAAGCTCAAGAAGAAAGAGTTCGACATCAGTCAGCAGAACAGTAAAATTGA
  GGACGAGCAGGCCCTGGCTCTTCAGCTGCAGAAGAAACTGAAGGAAAACCAGGCACGCATCGAGGAGCTG
  GAGGAGGAGCTGGAGGCAGAGCGCACAGCCCGGGCTAAGGTGGAGAAGCTGCGCTCTGACCTGTCCCGGG
  AGCTGGAGGAGATCAGTGAGAGGCTGGAGGAGGCAGGCGGGGCCACATCCGTGCAGATAGAGATGAATAA
  GAAGCGCGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTGGAGGAGGCCACGCTGCAGCACGAGGCCACG
  GCGGCGGCCCTGCGCAAGAAGCATGCTGACAGCGTGGCGGAGCTGGGCGAGCAGATCGACAACCTCCAGC
  GGGTGAAGCAGAAGCTGGAGAAAGAGAAGAGCGAGTTCAAGCTGGAGCTGGATGACGTCACCTCCAACAT
  GGAGCAGATCATCAAGGCCAAGGCCAACCTGGAGAAAGTGTCCCGGACACTGGAGGACCAGGCCAATGAG
  TACCGCGTGAAGCTGGAAGAAGCCCAGCGCTCCCTCAATGACTTCACCACACAGCGAGCCAAGCTGCAGA
  CAGAGAACGGGGAGTTGGCTAGGCAACTGGAAGAAAAGGAGGCATTGATTTCCCAGCTGACCCGAGGCAA
  GCTCTCCTACACCCAGCAGATGGAGGACCTCAAGAGGCAACTGGAGGAGGAAGGCAAGGCCAAGAACGCC
  CTGGCCCACGCACTGCAATCATCCCGGCATGACTGTGACCTGCTGAGGGAACAGTATGAAGAAGAAATGG
  AGGCCAAGGCTGAGCTACAGCGTGTCCTGTCCAAGGCCAACTCAGAGGTGGCCCAGTGGAGGACCAAGTA
  TGAGACGGATGCCATACAGAGGACGGAGGAGCTGGAGGAAGCCAAGAAGAAGCTGGCTCAGAGGCTGCAG
  GATGCAGAGGAGGCAGTGGAGGCCGTCAACGCCAAGTGTTCCTCCCTGGAGAAGACCAAGCACAGGCTGC
  AGAATGAGATCGAGGACCTGATGGTGGACGTGGAGCGCTCCAATGCCGCCGCCGCAGCCCTGGACAAGAA
  GCAGAGGAACTTTGACAAGATCCTGGCTGAGTGGAAGCAGAAGTATGAGGAGTCGCAGTCAGAGCTGGAG
  TCTTCCCAGAAGGAGGCGCGCTCCCTGAGCACAGAGCTCTTCAAGCTCAAGAACGCCTATGAGGAGTCTC
  TGGAGCACCTGGAGACCTTCAAGCGGGAGAACAAGAACCTCCAGGAGGAGATCTCAGACCTGACTGAACA
  GCTGGGAGAAGGGGGGAAAAACGTGCACGAGCTGGAGAAGATCCGCAAACAGCTGGAGGTGGAGAAGCTG
  GAGCTGCAGTCAGCCCTGGAGGAGGCTGAGGCCTCCCTGGAGCACGAGGAGGGCAAGATCCTCCGTGCCC
  AGCTGGAGTTCAACCAGATCAAGGCAGAGATCGAAAGGAAGCTGGCAGAGAAGGATGAGGAGATGGAGCA
  GGCCAAGCGCAACCACCTGCGGATGGTGGACTCCCTGCAGACCTCCCTGGATGCGGAGACACGCAGCCGC
  AATGAGGCCCTGCGGGTGAAGAAGAAGATGGAGGGCGACCTCAACGAGATGGAGATCCAGCTCAGCCAGG
  CCAATAGAATAGCCTCAGAGGCACAGAAACACCTGAAGAATTCTCAAGCTCACTTGAAGGACACCCAGCT
  CCAGCTGGATGATGCTGTCCATGCCAATGACGACCTGAAGGAGAACATCGCCATCGTGGAACGGCGCAAC
  AACCTGCTGCAGGCGGAGCTGGAGGAGCTGCGGGCTGTGGTGGAGCAGACGGAGCGGTCTCGGAAGCTGG
  CAGAGCAGGAGCTGATTGAGACCAGCGAGCGGGTGCAGCTGCTGCACTCGCAGAACACCAGCCTCATCAA
  CCAGAAGAAGAAGATGGAGTCAGACCTGACCCAACTCCAGACAGAAGTAGAGGAGGCAGTGCAGGAGTGT
  AGGAACGCAGAGGAGAAGGCCAAGAAGGCCATCACAGATGCCGCAATGATGGCTGAGGAGCTGAAGAAGG
  AGCAGGACACCAGCGCCCACCTGGAGCGCATGAAGAAGAACATGGAGCAGACCATCAAGGACTTGCAGCA
  CCGTCTGGACGAGGCAGAGCAGATCGCCCTCAAGGGCGGCAAGAAGCAGCTGCAGAAGCTGGAGGCCCGG
  GTCCGGGAGCTGGAGAATGAGCTGGAGGCTGAGCAGAAGCGCAATGCAGAGTCGGTGAAGGGCATGAGGA
  AGAGCGAGCGGCGCATCAAGGAGCTCACCTACCAGACAGAGGAAGACAAGAAGAACTTAATGCGGCTGCA
  GGACCTGGTGGACAAGCTACAGTTGAAGGTGAAGGCCTACAAGCGCCAGGCTGAGGAGGCGGAGGAGCAG
  GCCAACACCAACCTGTCCAAGTTCCGCAAGGTGCAGCACGAGCTGGATGAGGCGGAGGAGAGGGCGGACA
  TCGCCGAGTCCCAGGTCAACAAGCTGCGGGCCAAGAGCCGGGACATTGGTGCCAAGAAGATGCACGACGA
  GGAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010856
• Insert Size: 5817 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010856.4, NP_034986.1
• RefSeq Size: 6015 bp
• RefSeq ORF: 5817 bp
• Locus ID: 17888
• UniProt ID: Q02566
• Cytogenetics: 14 28.01 cM
• Gene Summary: Muscle contraction.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.