Dock1 (NM_001033420) Mouse Untagged Clone

CAT#: MC224993

Dock1 (untagged) - Mouse dedicator of cytokinesis 1 (Dock1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dock1
• Synonyms: 9130006G06Rik; AI854900; D630004B07Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224993 representing NM_001033420
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCGCTGGGTCCCCACCAAGCGAGAGGAGAAGTACGGCGTGGCCTTTTACAACTACGATGCCAGGG
  GAGCAGATGAACTGTCCCTTCAAATTGGAGACACCGTGCACATCCTAGAAACTTATGAAGGGTGGTACAG
  AGGCTACACCTTAAGGAAAAAGTCTAAAAAGGGTATATTTCCTGCTTCATATATTCATCTTAAAGAAGCC
  ATTGTTGAAGGAAAAGGGCAACATGAAACCGTCATCCCTGGGGACCTCCCCCTTATACAGGAAGTCACCA
  CAACGCTTCGAGAGTGGTCTACCATCTGGAGGCAACTTTATGTGCAAGATAATAGAGAGATGTTTCGAAG
  TGTGCGACACATGATCTATGACCTTATTGAATGGCGGTCGCAGATCCTTTCTGGAACTCTGCCCCAGGAT
  GAGCTCAAAGAGCTGAAGAAGAAGGTCACAGCCAAAATTGATTATGGAAACAGAATTCTTGATTTGGACC
  TGGTGGTGAGGGATGAAGATGGGAACATTTTGGACCCGGAACTAACTAGCACCATCAGTCTCTTCCGAGC
  ACACGAAGTTGCTTCTAAACAAGTGGAGGAGAGACTGCAAGAGGAGAAATCTCAAAAGCAGAACATGGAT
  ATCAACAGACAAGCCAAGTTTGCAGCCACCCCATCGCTGGCCTTGTTTGTTAACCTCAAAAATGTGGTTT
  GTAAAATAGGAGAAGATGCTGAAGTTCTCATGTCTCTTTATGACCCCATGGAGTCCAAATTCATCAGTGA
  GAACTATCTGGTCCGCTGGTCAAGTTCAGGATTACCAAAAGATATTGATCGGTTACATAACTTGCGAGCT
  GTGTTTACGGATCTGGGGAGCAAAGACTTGAAAAGGGAGAAAATTAGTTTCGTCTGCCAGATTGTCCGTG
  TCGGTCGCATGGAGCTGAGGGACAGCAACACGAGGAAGCTGACTTCGGGGCTGCGGCGACCTTTCGGCGT
  GGCTGTGATGGATGTGACAGACATAATAAACGGGAAAGTCGATGATGAAGATAAACAGCATTTCATCCCC
  TTCCAGGCGGTGGCAGGGGAGAATGACTTCCTTCAAACTGTCATAAACAAAGTCATTGCAGCCAAAGAAG
  TCAACCACAAGGGACAAGGTTTGTGGGTAACCTTGAAATTACTTCCTGGAGACATCCATCAGATTCGGAA
  GGAGTTTCCTCACTTGGTGGACAGGACCACAGCTGTGGCCCGCAAGACAGGGTTTCCTGAGATTATTATG
  CCAGGTGATGTTCGAAATGATATCTATGTGACATTAGTTCAAGGGGATTTTGATAAAGGCAGTAAAACAA
  CTGCGAAGAACGTGGAGGTGACAGTGTCTGTCTATGATGAAGATGGGAAGCGATTGGAGCACGTGATTTT
  CCCCGGTGCTGGCGACGAGGCGATCTCCGAGTACAAATCGGTGATTTATTATCAAGTGAAGCAGCCTCGC
  TGGTTTGAGACCCTTAAGGTGGCCATCCCCATTGAGGACGTGAACCGGAGTCACCTTCGATTCACCTTCC
  GCCATAGATCCTCGCAGGACTCCAAGGATAAATCTGAAAAGATCTTCGCACTGGCCTTTGTGAAGCTGAT
  GAGATACGATGGGACCACCCTGAGAGACGGGGAGCATGACCTTATTGTCTACAAGGCTGAAGCCAAAAAG
  CTCGAAGATGCAGCCACATACTTGAGCCTCCCTAGTACCAAGGGGGAGTTGGAAGAAAAGGGCCACTCAG
  CCACAGGCAAAGGAATGCAGAGTCTTGGGAGTTGTACCATCAGCAAAGACTCTTTCCAGATCTCCACTCT
  GGTGTGTTCCACGAAACTGACCCAGAACGTGGACCTCCTGGGGCTGTTGAAGTGGAGATCCAACACCAAC
  CTTCTGCAGCAGAACTTGAGGCAGTTGATGAAGGTGGACGGTGGTGAAGTGGTGAAGTTCCTTCAGGACA
  CCTTGGATGCCCTCTTCAACATCATGATGGAGAACTCAGAGAGTGAGACGTTTGATACCTTGGTGTTTGA
  TGCTCTGGTCTTTATTATTGGACTAATCGCGGATAGGAAATTTCAGCATTTTAATCCTGTTTTGGAGACT
  TACATTAAGAAACACTTCAGTGCAACATTAGCCTACACGAAGCTGACCAAGGTGTTACGGACCTACGTGG
  CCAGCGCAGAGAAGCCGGGAGTCAACGAGCAGCTGTACAAAGCCATAAAAGCCTTGGAGTACATCTTCAA
  GTTCATAGTGCGCTCACGGGTACTGTTCAACCAGCTGTATGAAAACAAGGGGGAAGCTGACTTCGTGGAG
  TCCTTGTTGCAGCTGTTCCGGTCCATCAACGACATGATGAGCAGCCTGTCAGAGCTCACAGTCAGGGTGA
  AGGGGGCAGCCCTGAAGTACTTGCCGACGATCGTCAATGATGTGAAGTTGGTGTTTGATCCCAAGGAGCT
  CAGCAAAATGTTCACGGAATTCATCCTGAATGTCCCTGCGGGCTTGCTGACGGTCCAGAAGCTCTCCTGC
  CTGATTGAGATTGTTCACAGTGACCTCTTCACACAGCATGACTGCAGAGAGATCCTGCTCCCCATGATGA
  CAGACCAGCTCAAGTACCACCTGGAGAGGCAGGAGGAGCTGGAGGCTTGTTGCCAGCTGCTTAGCAACAT
  CCTGGAGGTGCTATACCGGAAGGATGTGGGACCGACTCAGAGGCACGTCCAGATCATCATGGAGACGCTG
  CTTAGGACTGTGAACAGGACGGTCATTTCCATGGGGCGGGACTCCGAGCTCATTGGGAACTTTGTGGCTT
  GCATGACAGCCATTTTACGGCAGATGGAAGACTACCATTATGCACACCTGATCAAGACCTTTGGGAAAAT
  GAGGACAGATGTCGTGGATTTTCTGATGGAGACATTCATCATGTTTAAGAACCTCATCGGGAAGAACGTC
  TACCCCTTCGACTGGGTGATAATGAACACGATGCAGAACAAAGTCTTTCTGCGGGCGATTAATCAGTATG
  CAGATATGCTGAACAAGAGATTTCTGGATCAAGCCAACTTTGAGCTGCAGCTGTGGAACAACTACTTTCA
  CCTGGCCGTGGCTTTCCTCACTCAAGAGTCCCTGCAACTGGAGAATTTTTCAAGTGCTAAGAGAGCTAAA
  ATCCTTAACAAGTATGGAGACATGAGGAGGCAGATTGGCTTTGAGATCAGAGACATGTGGTACAACCTGG
  GTCAACACAAGATAAAATTCATCCCAGAAATGGTTGGCCCAATATTGGAAATGACATTGATTCCTGAGAC
  AGAGCTTCGAAAAGCCACCCTTCCCATCTTCTTCGACATGATGCAGTGTGAATTCCACTCCACCCGGAGC
  TTTCAAATGTTTGAAAATGAGATCATCACTAAGCTGGATCATGAAGTGGAAGGAGGCAGAGGAGACGAGC
  AATACAAAGTGTTATTTGATAAAATCCTCCTGGAGCACTGCAGGAAGCATAAATACCTCGCCAAGACAGG
  AGAAACTTTTGTGAAGCTCGTAGTGCGGTTGATGGAGAGGCTCCTGGACTACAGGACCATCATGCATGAC
  GAAAACAAAGACAATCGGATGAGCTGCACCGTCAACGTGCTGAATTTCTACAAAGAAATTGAAAGAGAAG
  AGATGTATATAAGGTACTTGTATAAGCTCTGTGACCTGCACAAAGAGTGTGACAACTACACAGAGGCGGC
  CTACACTTTGCTTCTGCACGCGAAACTTCTAAAGTGGTCAGAGGACGTGTGTGCAGCTCACCTCACTCAG
  CGGGACGGGTTCCAGGCGACAACACAGGGACAGCTGAAAGAGCAGCTCTACCAAGAAATCATCCACTACT
  TTGACAAAGGCAAGATGTGGGAGGAGGCCATCGCCCTGGGCAAGGAACTTGCGGAGCAGTACGAAACAGA
  GATGTTTGATTATGAGCAGCTCAGTGAATTGCTGAAAAAACAGGCACAATTTTACGAGAACATCGTGAAG
  GTGATAAGACCCAAGCCTGACTATTTTGCTGTTGGCTACTATGGGCAAGGGTTCCCGTCATTCCTTCGGG
  GCAAAGTGTTCATTTACCGAGGCAAGGAATATGAGCGACGGGAAGATTTTGAAGCTCGGCTGTTAACTCA
  GTTTCCAAATGCTGAAAAAATGAAGACGACGTCTCCACCGGGCGATGACATTAAGACTTCTCCCGGCCAG
  TACATCCAGTGCTTCACAGTGAAGCCCAAGCTAGACTTACCTCCGAGATTTCACCGACCCGTGTCTGAAC
  AGATTGTAAGTTTTTACAGGGTGAATGAGGTACAGCGATTCGAGTATTCACGGCCCATCCGGAAAGGAGA
  GAAAAACCCAGACAATGAATTTGCGAATATGTGGATTGAAAGAACCATCTACACTACGGCCTATAAACTG
  CCAGGAATTTTAAGGTGGTTTGAAGTCAAATCTGTCTTCATGGTAGAGATCAGCCCACTGGAGAATGCCA
  TCGAGACAATGCAGTTGACCAATGACAAGATCAGCAGCATGGTCCAGCAGCACCTGGATGACCCGGGCCT
  GCCTATCAACCCCCTGTCCATGCTCCTGAATGGCATTGTGGATCCTGCTGTCATGGGTGGTTTTGCCAAT
  TATGAGAAGGCTTTCTTCACAGACAGATACCTGCAGGAGCATCCTGAGGCCCATGGGCAGATTGAGAAGC
  TCAAGGACCTGATAGCCTGGCAGATTCCATTTTTGGCTGAAGGGATCAGGATTCATGGAGACAAAGTCAC
  AGAGGCCCTGAGGCCATTCCACGAGCGCATGGAAGCCTGCTTCAAGCAACTGAAGGAAAAAGTGGAAAAG
  CAATATGGCGTCAGGACCATGCCCTCAGGCTTGGATGACAGGAGAGGCAGCCGTCCACGTTCAATGGTGC
  GATCTTTCACCATGCCATCATCCTCCCGGCCCCTGTCAGTGGCTTCCGTCTCTTCTTTCTCCTCTGACAG
  CACTCCTTCCAGGCCAGGCTCTGATGGATTTGCTCTAGAGCCTCTGCTGCCGAAGAAGATGCACTCCAGG
  TCCCAGGACAAGCTAGACAAGGACGATCCAGATAAAGAGAAGAAGGACAAGAAGAAGGAGAAAAGGAACA
  GCAAACACCAAGAGATATTTGATAAGGAATTCAAGCCCGCTGACAGCTCCCTGCAGCAGTCTGAGGCTGT
  GATCCTTTCTGAAACGATAAGTCCCCTGCGGCCCCAAAGACCCAAGAGCCAGGTGATCAATGTCATTGGA
  AATGAAAGGCGCTTCTCTGTGTCTCCAGCATCACCGTCCTCCCAGCAGACGCCCCCTCCAGTCACACCAA
  GAGCCAAGCTCAGCTTCAGCATTCAGCCCAGTCTGGAGCTGAACGGCATGATGGGAATGGATGTGGCCGA
  TGTCCCACCCCCTCTGCCCCTCAAAGGCAACATGGCAGATTATGGAAATCTGATGGAAAACCAGGATATG
  ATGGTCTCACCCACATCTCCACCTCCTCCTCCCCCACAACGGCAACAGCCACCTCCACTGCCCAGCAAAA
  CACCACCTCCTCCCCCTCCGAAGACGACCCGAAAGCAGACCTCAGTGGACTCTGGAATCGTGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033420
• Insert Size: 5598 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033420.2, NP_001028592.1
• RefSeq Size: 6815 bp
• RefSeq ORF: 5598 bp
• Locus ID: 330662
• UniProt ID: Q8BUR4
• Cytogenetics: 7 F3
• Gene Summary: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.[UniProtKB/Swiss-Prot Function]