Sbf1 (NM_001081030) Mouse Untagged Clone

CAT#: MC224992

Sbf1 (untagged) - Mouse SET binding factor 1 (Sbf1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sbf1
• Synonyms: 2610510A08Rik; B230113C15Rik; mKIAA3020; Mtmr5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224992 representing NM_001081030
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGCTCGCGGACTACTTCGTGCTGGTGGCGTTCGGGCCGCACCCGCGCGGGAGTGGGGAAGGCC
  AGGGCCAGATCCTGCAGCGCTTCCCGGAGAAGGACTGGGAGGACAACCCTTTCCCCCAGGGCATTGAGCT
  GTTTTGCCAGCCCAGTGGGTGGCAGCTATGTCCCGAGAGGAATCCACCAACTTTCTTTGTGGCTGTCCTT
  ACTGACATCAACTCTGAGAGGCACTACTGCGCCTGCTTGACCTTCTGGGAGCCAGTGGAGTCCACACAGG
  AAGTAGTGTGCACTGACAATGCCACGGAGAAGGAGGAAGAGGCAGATGGAGGAGGCCAAGCGCGGCTGTC
  ATCCACAGCCCCAGCCCAGCCTGGCCAGCTTTTTGCGCCAAAGACTCTGGTTCTGGTGTCTCGACTGGAC
  CATGCCGAAGTGTTCAGGAATAGCCTTGGTCTCATCTATGCTATCCACGTGGAAGGCCTGAATGTGAGCC
  TCGAGAATGTGATCGGGAACCTGCTCACATGTACCGTCCCATTGGCTGGCGGGTCTCAGAGAACCATCTC
  TTTGGGGGCTGGTGACCGGCAGGTCATTCAGACTCCACTGGTAGACTCACTGCCTGTCAGCCGCTGTAGT
  GTAGCCCTGCTCTTCCGCCAGCTGGGTATCACCAATGTGCTGTCTTTGTTCTGTGCTGCCCTTACTGAGC
  ACAAAGTCCTCTTCTTGTCCAGGAGCTACCAACGTCTAGCAGATGCTTGCAGGGGTCTCTTGGCATTGCT
  GTTCCCTCTCAGATACAGCTTCACTTATGTGCCCATCTTGCCGGCACAGCTGCTGGAAGTCCTTAGCACA
  CCTACGCCTTTCATCATTGGGGTCAATGCAGCCTTCCAGGCAGAGACTCAGGAGCTGCTGGATGTGATTG
  TTGCTGATCTTGACGGAGGGACAGTGACTGTCCCTGAGTGTGTGCACATTCCACCCCTGCCAGAGCCACT
  ACAAAGCCAGACCCACAATGTTCTGAGCATGGTCCTGGATCCAGAACTGGAGTTGGCTGACCTTGCCTTC
  CCACCCCCTACAACATCTGCTTCCTCCCTGAAAATGCAGGACAAGGAGCTCCGTGCTGTCTTTCTGCGGC
  TCTTTGCTCAGCTCCTGCAAGGCTACCGCTGGTGCCTGCACATCGTGCGCATCCACCCAGAGCCCGTCAT
  CCGCTTCCATAAGGCAGCATTCTTAGGCCAGCGTGGACTGGTGGAGGATGATTTCCTGATGAAAGTATTG
  GAGGGCATGGCCTTCGCGGGCTTTGTATCTGAGCGTGGGGTTCCCTACCGTGCCACAGACCTGTTTGATG
  AGCTGGTGGCTCATGAGGTAGCACGGATGCGAGCAGATGAGAGCCATCCCCACCGTGTCCTGCGTCATGT
  CCAGGAACTGGCAGAGCAACTTTATAAGAATGAAAACCCGTACCCAGCTGTGGCGATGCACAAAGTGCAG
  AGGCCAGGAGAGGCCAGTCACCTGCGGCGGACCCACCGGCCATTCCCCCGGCTAGATGAGGGCACAATTC
  AGTGGATTGTGGACCAGGCTGCGGCCAAGATGCAGGGCGCACCTCCAGCTGTCAAGGCTGAGCGGAGGAG
  CACTGTGCCTTCAGGGCCCCCCATGACGGCCATACTGGAGCGCTGTAGTGGGCCTCATATCAACAGTGCA
  CGTCGCTTGGAGGTAGTACGGAATTGCATCTCCTATGTGTTTGAAGGGAAAATGCTTGAGGCAAAGAAGT
  TGCTTCCGGCTGTACTCAGGGCCCTGAAGGGGCGAGCTGCCCGTCGCTGCCTTGCCCATGAGCTTCACCT
  GCACGTACAGCAGAACCGGGCAGTTCTGGATCATCAGCAGTTTGACTTTGTCGTCCGCATGATGAATTGC
  TGCCTGCAGGATTGCACTTCCCTGGATGAGCATGGCATTGCATCTGCACTGCTGCCTTTGGTCACAGCCT
  TCTGCAGGAAGCTGAGCCCAGGGGTGACACAGTTTGCATACAGCTGTGTTCAGGAGCACGTAGTGTGGAG
  CACACCTCAGTTCTGGGAGGCCATGTTCTATGGGGATGTGCAGACCCATATCCGAGCCCTCTACCTGGAG
  CCCTCTGATGGCGTGAGCCCCACCCAGGAGACTGGGGAGGCACAGTCTCAGGATGATGAACGATCTGCCC
  TGGATGTGGCTTCAGAGCAGAGGCGCCTGTGGCCAACCCTGAGCCGTGAGAAGCAGCAGGAGCTGGTACA
  GAAGGAGGAAAGCACTGTGTTCAGCCAGGCCATCCACTATGCCAACCGCATGAGCTACCTTCTGCTGCCT
  CTGGACAGCAGCAAGAGCCGGCTGCTGCGGGAGCGGGCAGGGTTGGGAGACCTGGAGAGTGCCAGCAACA
  GCCTGGTCACCAACAGCATGGCAGGCAGTGTGGCTGAGAGCTATGACACAGAGAGTGGCTTTGAAGATGC
  AGAGACGTGCGATGTGGCTGGGGCTGTAGTCCGCTTCATCAACCGCTTTGTGGACAAAGTCTGCACAGAG
  AGTGGGGTCACCAGCGACCACCTCAAAGGACTGCATGTCATGGTGCCAGACATTGTCCAGATGCACATTG
  AGACCCTGGAGGCCGTACACCGTGAGAGCAAGAGGCTGCCCCCGATACAGAAGCCCAAGCTGCTGAGGCC
  ACGCCTGTTGCCTGGTGAGGAGTGTGTCCTCGATGGCCTTCGAGTGTATCTGCTGCCAGACGGGCGTGAG
  GAGGGTGTAGGAGGCAGTGGAGGGGGCCCTGCTCTACTCCCAGCTGAGGGTGCTGTCTTCCTTACCACAT
  ACCGCGTCATCTTCACGGGGATGCCTACTGACCCCCTGGTGGGGGAGCAGGTGGTAGTCCGCTCCTTCCC
  CGTGGCTGCCTTGACCAAGGAGAAGCGCATTAGTGTGCAGACCCCTGTGGACCAGCTTCTGCAGGATGGG
  CTGCAGCTTCGTTCCTGCACATTCCAGCTGCTGAAAATGGCCTTTGATGAGGAGGTGGGATCTGACAGTG
  CTGAGCTCTTCCGAAAGCAGCTACACAAGCTTCGGTACCCACCAGACATCAGGGCCACCTTTGCATTCAC
  ACTTGGCTCAGCTCACACACCTGGCAGGCCGCCACGGGTTACCAAGGACAAGGGTCCTTCATTCAGAACC
  CTATCCCGGAACCTGATGAAGAATGCTAAAAAGACCATTGGGCGGCAGTATGTTACTCGTAAGAAGTATA
  ACCCCCCTGGCTGGGAGCATCGGGGCCAGCCACCCCCTGAGGACCAGGAGGACGAGATCTCAGTGTCAGA
  GGAGCTGGAGCCTAGCACACTGACCCCGTCCTCAGCCCTGAAGCCCTCTGACCGCATGACCATGAGCAGC
  CTGGTGGAACGGGCATGTTGCCGTGACTACCAGCGCCTTGGACTAGGTACCCTGAGCAGCAGCCTGAGCC
  GGGCCAAGTCTGAGCCCTTTCGCATCTCTCCTGTTAATCGCATGTATGCCATATGTCGCAGCTATCCAGG
  ATTGCTGATTGTTCCCCAGAGCATCCAGGACAACGCCCTGCAGCGTGTATCCCGCTGCTACCGCCAGAAC
  CGCTTCCCTGTGGTCTGCTGGCGCAGTGGGCGCTCCAAGGCTGTCTTGCTACGCTCTGGGGGCCTGCATG
  GCAAAGGTGTTGTTGGTCTCTTCAAGGCCCAGAATACACCTTCTCCAGGCCAGGCCCAGGCCGACTCCAG
  CAGCCTGGAGCAAGAGAAGTACCTGCAGGCTGTGGTCAGCTCCATGCCACGTTATGCTGACTCATCAGGA
  CGAAACACACTGAGTAGCTTCTCCTCAGCCCACATGGGTGGCCATGGGAAATGGAGCAGTGTCCGAGCCA
  GTGGTCGAAGCAGTGGTCTTGGTTCTGATGTGGGCTCTCGGCTAGCTGGCAGAGATCTCCTCAGTACTCC
  CCACACCAACGGAGCTCCACCTGATTCTGGTTTTCTACGGCCACAGCGTGCAGCCCTCTACATCATTGGT
  GACAAAGCTCAGCTCAAGGGTGTGCGCCCAGATCCCTTGCAACAGTGGGAGCTGGTACCTATTGAAGTAT
  TTGAGGCAAGGCAGGTGAAAGCCAGCTTCAAGAAACTATTGAAAGCCTGTGTGCCTGGTTGCCCTGCCAC
  TGAGCCCAGCCCAGCCTCTTTTCTGCGCTCACTGGAGGACTCAGAATGGCTGATCCAGATCCACAAGCTG
  CTGCAGATATCGGTGCTGGTAGTGGAGCTGCTCGACTCTGGCTCCTCTGTCCTGGTGAGCCTGGAGGACG
  GCTGGGACATCACCACTCAGGTGGTATCCTTGGTGCAGCTGCTCTCAGACCCCTTCTACCGCACTCTGGA
  AGGCTTCCGACTGCTAGTGGAAAAAGAGTGGCTGTCCTTTGGCCATCGTTTCAGCCACCGCGGGGCCCAC
  ACCCTAGCTGGGCAGAGCAGTGGCTTCACACCCGTCTTCCTGCAGTTCCTGGACTGTGTACACCAGGTCC
  ATTTGCAGTTCCCCATGGAGTTTGAGTTCAGTCAGTTCTACCTCAAGTTCCTTGGTTACCACCACACATC
  CCGCCGTTTCCGGACCTTCTTGCTGGACTCTGATTATGAGCGTATTGAGCTGGGGCTGCTGTATGAGGAG
  AAGGGTGAACGCAGGGGTCAGCTGGCCTGCAAGTCAGTGTGGGAGTATGTAGACCGGCTAAGCAAGAGGA
  CCCCCATGTTCTACAACTACACATATGCACCCGAGGACACAGAGGTGCTGCGACCCTACAGCAATGTGTC
  CAATCTGAAGGTGTGGGATTTCTACACTGAGGAGACGCTGGCTGAGGGGCCCCCTTATGACTGGGAGCTG
  GCCCAGGGACCCCCTGAGCCTCCAGAAGAGGAACGTCCTGATGGAGGTGCTCCCCAGAGCAGGCGCCGTG
  TGGTATGGCCATGCTATGACAGCCGTCCTCGAGTCCAGCCTGATGCCATCTCACGTCTGCTGGAGGAGCT
  GCAGCGCTTGGAGACAGAGTTAGGTCGGCCTTCTGAACGCTGGAAAGACACCTGGGACCGGGTGAAGGCT
  GCTCAGCGCCTTGAAAGCCGGCAAGATGGACGTGGTACCCCCAGCTCACTTCTGGTGTCGGCTGTGCCCC
  ACCACCGCCGCTCGCTAGGCGTCTATCTACAGGAGGGGCCTGTGGGCTCTACTCTTAGCCTCAGCCTGGA
  CAGTGACCAGAGCAGTGGCTCAACCACATCTAGCTCTCGGCAGGCCGCCCGGCGGAGCACCAGCACCCTG
  TACAGCCAGTTCCAGACAGCTGAGAGTGAGAACAGGTCCTATGAGGGCATCCTATACAAGAAGGGGGCCT
  TCATGAAGCCCTGGAAAGCCCGTTGGTTTGTCCTAGACAAGACCAAGCACCAGCTGCGATACTATGACCA
  CCGAATGGACACAGAATGCAAGGGTGTCATTGACCTGGCAGAGGTGGAAGCTGTGGCACCTGGCACACCC
  ACTATAGGTGCCCCTAAGACTGTGGATGAGAAGGCCTTCTTTGATGTGAAGACAACACGTCGCGTTTACA
  ACTTCTGTGCCCAGGATGTGCCCTCAGCCCAGCAGTGGGTGGACCGGATCCAGAGCTGCCTGTCGGATGC
  CTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081030
• Insert Size: 5604 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081030.2, NP_001074499.2
• RefSeq Size: 6190 bp
• RefSeq ORF: 5604 bp
• Locus ID: 77980
• UniProt ID: Q6ZPE2
• Cytogenetics: 15 E3
• Gene Summary: Acts as an adapter for the phosphatase MTMR2 to regulate MTMR2 catalytic activity and subcellular location. May function as a guanine nucleotide exchange factor (GEF) activating RAB28. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to represent an alternate splice pattern that is more supported by the available transcript data.