Myo5a (NM_010864) Mouse Untagged Clone

CAT#: MC224981

Myo5a (untagged) - Mouse myosin VA (Myo5a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Myo5a
• Synonyms: 9630007J19Rik; AI413174; AI661011; d; d-120J; Dbv; flail; flr; MVa; Myo5; MyoVA; Sev-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224981 representing NM_010864
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGTCCGAGCTCTACACCAAGTTTGCCAGGGTTTGGATCCCTGATCCTGAGGAAGTGTGGAAAT
  CGGCAGAGTTGCTCAAGGATTATAAGCCTGGAGATAAAGTGCTCCTGCTTCACCTCGAGGAAGGGAAGGA
  TTTGGAATACCGTCTAGACCCAAAGACCGGTGAGCTCCCTCACTTACGGAACCCTGACATACTTGTTGGA
  GAAAATGACCTCACAGCCCTCAGCTACCTTCACGAGCCCGCTGTGCTACATAATCTCCGAGTTCGCTTCA
  TCGATTCCAAACTTATTTATACGTATTGTGGAATAGTTCTGGTAGCTATAAATCCCTATGAGCAGCTGCC
  TATTTATGGAGAAGATATTATTAATGCCTACAGTGGCCAGAACATGGGTGACATGGATCCTCACATCTTC
  GCAGTAGCTGAAGAGGCTTACAAGCAAATGGCAAGGGATGAACGAAATCAGTCCATCATTGTAAGTGGAG
  AGTCAGGCGCAGGGAAGACGGTCTCTGCTAAGTATGCCATGCGGTACTTCGCAACTGTAAGTGGCTCTGC
  CAGTGAGGCCAATGTTGAGGAAAAGGTCTTGGCCTCCAACCCCATCATGGAGTCAATTGGAAATGCCAAA
  ACAACCAGGAATGATAATAGCAGCCGGTTTGGAAAATATATTGAAATTGGTTTTGACAAGAGGTACAGAA
  TCATCGGTGCCAATATGAGAACTTACCTTTTAGAGAAATCCAGAGTGGTGTTCCAGGCAGAAGAGGAGAG
  AAACTACCATATCTTCTATCAGCTCTGTGCTTCGGCAAAGTTACCTGAGTTTAAAATGCTGCGGTTAGGA
  AATGCAGATAGTTTTCATTACACGAAGCAAGGAGGCAGCCCTATGATAGAAGGAGTAGATGATGCGAAGG
  AGATGGCGCACACCAGGCAGGCCTGCACTCTGCTGGGAATTAGTGAATCTTACCAAATGGGAATTTTTCG
  AATACTTGCTGGCATTCTTCACTTAGGCAATGTTGGATTTGCATCTCGGGATTCAGACAGCTGCACAATA
  CCTCCCAAGCACGAACCTCTTACCATCTTCTGTGACCTTATGGGTGTGGATTATGAAGAGATGTGTCACT
  GGCTCTGCCACCGAAAGCTGGCTACTGCCACAGAGACATACATCAAGCCCATCTCCAAGCTGCAGGCCAC
  AAATGCCCGAGATGCTTTAGCAAAGCACATCTATGCAAAGCTCTTTAACTGGATTGTTGACCACGTCAAT
  CAGGCTCTCCATTCTGCTGTCAAGCAGCACTCTTTCATCGGCGTGCTGGACATCTATGGATTTGAAACAT
  TTGAAATAAATAGTTTTGAACAGTTCTGCATAAATTATGCAAATGAAAAACTACAACAACAATTCAACAT
  GCATGTCTTCAAATTGGAACAGGAGGAATATATGAAGGAACAGATTCCATGGACACTTATAGATTTCTAT
  GATAATCAGCCTTGTATCAATCTTATAGAATCTAAACTGGGAATTCTCGATTTGCTGGATGAGGAATGTA
  AGATGCCTAAAGGCACAGATGACACATGGGCCCAAAAACTGTACAACACACATTTGAACAAATGTGCCCT
  CTTTGAGAAGCCCCGCATGTCAAACAAAGCTTTCATCATCAAACATTTTGCTGACAAAGTGGAGTACCAG
  TGTGAAGGTTTTCTTGAAAAGAATAAAGATACTGTTTTTGAAGAACAAATTAAAGTCCTTAAGTCAAGCA
  AGTTTAAGATGCTACCAGAGCTATTTCAAGATGATGAGAAGGCCATCAGTCCCACCTCTGCCACCTCCTC
  AGGGCGCACTCCTCTCACTCGAGTTCCTGTAAAGCCCACCAAGGGCCGACCTGGCCAGACTGCCAAAGAG
  CACAAGAAGACAGTGGGACATCAGTTTCGAAACTCCCTTCACCTGCTTATGGAAACCCTTAATGCCACTA
  CTCCTCACTATGTACGCTGTATTAAGCCTAATGATTTCAAGTTTCCATTCACATTTGATGAGAAGAGGGC
  AGTGCAGCAGCTAAGAGCATGTGGTGTCCTGGAGACCATCCGGATCAGCGCAGCAGGGTTTCCCTCACGG
  TGGACTTACCAAGAGTTTTTCAGCCGGTACCGGGTCCTAATGAAGCAAAAAGATGTGCTGGGAGATAGAA
  AGCAAACGTGCAAGAATGTATTAGAGAAACTAATATTGGACAAGGATAAATACCAGTTTGGTAAGACAAA
  GATCTTTTTCCGTGCTGGTCAAGTGGCCTATCTTGAAAAATTGAGGGCTGACAAACTTCGGGCTGCCTGC
  ATCCGGATCCAGAAGACCATTCGTGGGTGGCTTCTAAGGAAGAGATACCTGTGTATGCAGAGGGCAGCCA
  TCACAGTGCAGCGATACGTGCGGGGCTATCAGGCTCGATGCTATGCTAAGTTTCTGCGCAGAACCAAGGC
  AGCAACCACCATTCAAAAGTACTGGCGCATGTATGTGGTCCGCAGGAGGTACAAGATTAGACGAGCTGCC
  ACGATTGTTATTCAGTCTTACTTGAGAGGCTACTTGACAAGAAATAGGTATCGCAAGATACTCCGTGAAT
  ACAAAGCAGTCATCATTCAGAAACGTGTCCGTGGCTGGCTGGCCCGTACACATTATAAGAGGACCATGAA
  AGCCATCGTCTACCTTCAGTGCTGCTTCCGGCGGATGATGGCCAAGCGTGAGCTGAAGAAACTCAAAATT
  GAGGCTCGCTCTGTGGAACGCTACAAGAAGCTCCATATTGGCATGGAGAACAAGATTATGCAGCTGCAGC
  GCAAAGTGGATGAGCAGAATAAAGACTACAAATGCCTCATGGAGAAACTGACCAATCTGGAAGGAGTATA
  CAACTCTGAGACTGAAAAACTACGAAATGATGTAGAACGTCTTCAGCTAAGTGAAGAGGAAGCTAAGGTT
  GCCACTGGGCGGGTCCTTAGTCTGCAGGAAGAAATTGCAAAACTCCGAAAAGACCTGGAACAAACTCGAT
  CAGAGAAAAAGTCTATTGAAGAAAGAGCAGATAAATACAAACAAGAAACTGACCAGCTGGTGTCAAACTT
  GAAGGAAGAAAATACTTTGCTGAAGCAGGAAAAGGAGACCCTCAACCACCGCATTGTGGAGCAGGCGAAG
  GAGATGACAGAAACTATGGAGAGGAAGTTAGTAGAAGAGACAAAACAACTGGAGCTTGACCTGAATGATG
  AGAGGCTGAGGTATCAGAACCTTCTGAATGAGTTCAGTCGCCTGGAGGAGCGCTATGATGACCTCAAGGA
  AGAGATGACCCTGATGCTGAATGTGCCTAAGCCAGGACACAAGAGAACAGACTCTACCCACAGCAGTAAT
  GAGTCTGAATACACCTTCAGCTCTGAGTTTGCAGAAACTGAAGACATTGCACCAAGAACAGAGGAGCCAA
  TTGAGAAGAAGGTGCCTCTGGATATGTCACTATTCCTTAAGCTCCAGAAGCGTGTCACAGAGCTGGAACA
  GGAGAAGCAGCTGATGCAGGATGAGCTGGACCGCAAGGAGGAGCAGGTGTTCCGCAGCAAGGCAAAGGAA
  GAAGAAAGGCCACAGATAAGAGGAGCTGAACTAGAGTATGAGTCTCTCAAGCGTCAAGAACTGGAGTCAG
  AAAACAAAAAACTGAAAAACGAGCTGAATGAGTTGCGCAAAGCCCTCAGTGAGAAAAGTGCCCCAGAAGT
  GACTGCGCCAGGTGCGCCTGCTTACCGAGTCCTCATGGAACAGCTGACCTCCGTGAGCGAGGAGCTCGAC
  GTGCGCAAGGAGGAAGTCCTCATCTTGAGGTCGCAGCTGGTGAGCCAAAAAGAAGCCATCCAACCCAAGG
  ATGACAAGAATACAATGACAGATTCCACAATTCTTTTAGAAGATGTACAGAAAATGAAAGACAAAGGTGA
  AATAGCACAAGCATATATTGGTTTGAAAGAAACAAACAGGCTCTTAGAATCCCAGCTACAGTCACAGAAA
  AGAAGCCATGAGAATGAGGCTGAGGCCCTCCGTGGGGAGATCCAGAGCCTAAAGGAAGAAAACAACCGGC
  AACAGCAGCTGCTGGCCCAGAACCTGCAGCTGCCCCCTGAGGCCCGCATTGAGGCCAGCCTGCAGCATGA
  GATCACCCGGCTGACCAATGAAAACCTGTATTTTGAGGAATTATATGCAGATGACCCTAAGAAGTATCAA
  TCCTATCGGATTTCACTTTACAAAAGGATGATTGATCTGATGGAACAACTTGAAAAGCAGGATAAAACTG
  TCCGGAAACTGAAGAAACAACTGAAAGTCTTTGCCAAAAAAATTGGTGAACTAGAAGTGGGGCAGATGGA
  GAACATATCTCCAGGACAGATCATCGATGAGCCTATCCGGCCAGTCAACATTCCCCGGAAAGAAAAGGAT
  TTCCAAGGAATGCTGGAGTACAAAAGGGAGGATGAGCAGAAGCTAGTGAAGAACCTGATTCTAGAACTAA
  AGCCACGTGGTGTGGCTGTCAATCTGATTCCAGGGTTACCAGCATATATCTTGTTTATGTGTGTACGACA
  TGCTGACTATCTGAACGATGATCAGAAAGTAAGATCATTGCTGACATCAACAATTAACAGCATCAAAAAA
  GTCTTGAAGAAAAGAGGTGACGATTTTGAAACTGTCTCCTTCTGGCTCTCTAACACATGTCGATTTTTGC
  ACTGTTTGAAGCAATATAGTGGAGAGGAGGGCTTTATGAAGCACAACACGTCTCGCCAGAATGAGCACTG
  CCTCACCAATTTTGACCTTGCTGAATATCGGCAAGTACTGAGTGACTTGGCCATTCAGATCTATCAGCAG
  CTTGTGAGGGTGTTAGAGAACATTCTTCAGCCAATGATAGTCTCAGGTATGCTAGAGCATGAAACAATTC
  AGGGCGTATCTGGGGTGAAGCCCACAGGGCTCAGAAAACGAACCTCCAGTATCGCAGATGAGGGCACCTA
  CACACTGGACTCCATCCTTCGGCAGCTCAACTCCTTCCACTCAGTCATGTGTCAGCATGGCATGGACCCG
  GAGCTAATCAAGCAGGTAGTCAAACAGATGTTCTACATTGTGGGCGCCATCACCCTAAACAACCTCCTCC
  TGCGCAAGGACATGTGCTCCTGGAGCAAGGGCATGCAGATCAGGTACAATGTCAGTCAACTGGAAGAATG
  GCTACGTGACAAGAATCTAATGAACAGTGGGGCCAAGGAGACTCTGGAACCTCTTATCCAGGCCGCTCAG
  CTTTTGCAAGTGAAAAAGAAAACTGACGATGACGCAGAAGCCATCTGTTCCATGTGCAACGCCCTGACCA
  CGGCCCAGATTGTCAAAGTGTTGAATCTGTACACACCGGTTAATGAGTTTGAAGAAAGGGTCTCAGTTTC
  ATTTATCCGCACTATACAGATGCGGTTACGAGACAGGAAAGACTCTCCACAGCTGCTCATGGATGCAAAA
  CACATCTTTCCTGTCACTTTTCCCTTTAACCCATCCTCCCTAGCCCTAGAAACCATCCAGATCCCAGCCA
  GCCTGGGCCTGGGCTTCATCGCACGGGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010864
• Insert Size: 5562 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010864.2, NP_034994.2
• RefSeq Size: 11684 bp
• RefSeq ORF: 5562 bp
• Locus ID: 17918
• UniProt ID: Q99104
• Cytogenetics: 9 42.26 cM
• Gene Summary: Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.[UniProtKB/Swiss-Prot Function]