Rictor (NM_030168) Mouse Untagged Clone

CAT#: MC224849

Rictor (untagged) - Mouse RPTOR independent companion of MTOR, complex 2 (Rictor), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rictor
• Synonyms: 4921505C17Rik; 6030405M08Rik; AVO3; AW492497; D530039E11Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224849 representing NM_030168
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGATCGGCCGCGGCCGCTCTCTGAAGAACCTCCGAATACGAGGGCGGAATGACAGCGGCGAGG
  AGAACGTCCCGCTCGATCTGACCCGAGAACCTTCTGATAACTTGAGGGAGATTCTCCAAAATGTGGCCAA
  ATTGCAAGGAGTATCAAATATGAGAAAGCTGGGCCATCTGAATAACTTCACTAAGCTTCTTTGCGATATT
  GGCCATAGTGAAGAAAAACTGGGCTTTAATTATGAAGACATCATAATTTGTTTGCGCTTAGCTTTATTAA
  ATGAGGCAAAAGAAGTAAGAGCAGCCGGGTTGCGAGCGCTGCGCTATCTCATCCAAGATTCCAGTATTCT
  CCAGAAGGTGCTAAAACTGAAAGTGGACTATTTAATAGCTAGGTGCATTGACATTCAGCAGAGCAACGAG
  GTGGAGAGGACACAAGCCCTTCGCTTAGTCAGAAAGATGATTACTGTGAATGCTTCCTTGTTTCCTAGTT
  CTGTGGCCAACTCATTAATTGCGGTTGGAAATGATGGGCTTCAGGAACGAGACAGAATGGTCCGGGCTTG
  CATCGCCATCATCTGTGAACTAGCACTTCAGAACCCAGAGGTGGTAGCCCTTCGAGGAGGACTAAACACC
  ATACTGAAAAATGTGATTGATTGCCAATTAAGTCGAATAAATGAGGCTCTCATTACTACAATTTTGCACC
  TTCTTAATCATCCAAAGACCCGGCAGTATGTGCGAGCTGATGTAGAATTAGAGCGAATTTTAGCACCCTA
  TACTGATTTCCACTACAGACACAGTCCAGATACGGCTGAAGGACAGCTCAAAGAAGACAGAGAAGCACGA
  TTTCTAGCCAGTAAGATGGGAATCATTGCGACATTCCGCTCGTGGGCAGGTATTATTAATTTATGTAAGC
  CTGGAAATTCTGGGATACAGTCTCTAATTGGAGTACTTTGCATACCGAATATGGAAATAAGGCGAGGTCT
  GCTCGAAGTGCTCTATGACATCTTTCGTCTCCCTCTGCCCGTTGTGACGGATGAGTTCATAGAAGCCCTG
  CTCAGTGTAGATCCAGGAAGATTCCAGGACAGTTGGAGGCTTTCCGATGGCTTTGTGGCAGCTGAGGCAA
  AAACTATTCTTCCCCATCGTGCTAGATCCAGGCCAGACCTCATGGACAATTATTTGGCTCTGATACTCTC
  TGCATTTATTCGTAATGGACTTTTAGAGGGCTTGGTCGAGGTGATAACGAACAGTGACGACCACATCTCA
  GTGAGGGCGACCATCCTTTTAGGAGAGCTCCTGCATATGGCAAACACCATCCTGCCTCACTCGCATAGCC
  ACCATCTACACTGCTTGCCAACCCTCATGAACATGGCTGCGTCCTTCGATATTCCCAAGGAAAAGAGGCT
  GCGAGCCAGTGCAGCCCTGAACTGTTTGAACCGCTTCCATGAGATGAAGAAGCGAGGACCTAAGCCCTAC
  AGCCTTCATTTAGATCACATCATTCAGAAAGCAATCGCAACTCACCACAAGCGGGATCAGTATCTTCGAG
  TTCAGAAAGATATATTTGTTCTTAAGGATACAGAGGAAGCTCTTTTAATAAACCTTAGAGACAGCCAAGT
  CCTTCAGCATAAAGAGAATCTTGACTGGGATTGGAATCTGATTGGGACCATCCTTAAGTGGCCAAATGTA
  AATCTAAGAAACTATAAAGATGAGCAGTTGCACAGGTTTGTGCGCAGACTTCTTTACTTTTACAAGCCCA
  GCAGCAAACTGTACGCTAGTCTGGATCTGGACTTGGCCAAGTCCAAGCAGCTCACAGTTGTTGGTTGTCA
  GTTTACAGAATTTCTGCTCGAGTCTGAAGAGGATGGGCAAGGATACTTAGAAGATCTCGTGAAAGATATT
  GTTCAGTGGCTCAATGCTTCATCTGGAGTGAAACCTGAGAGAAGCCTTCAGAATAATGGCTTACTGACCA
  CCCTTAGTCAACACTACTTCTTATTTATCGGAACACTCTCTTGTCATCCTCATGGAGTCAAAATGCTGGA
  AAAGTGCAGTGTCTTTCAGTGTCTCCTTAATCTTTGTTCCTTGAAAAACCAAGATCATCTGATAAAACTC
  ACTGTTTCCAGCCTAGACTATAGCAGAGATGGATTAGCCAGAGTCATTCTTTCAAAAATCCTCACGGCAG
  CCACAGATGCTTGCAGACTGTATGCAACAAAACATTTAAGGGTTTTATTGAGAGCTAACGTTGAATTCTT
  CAACAATTGGGGAATTGAGTTACTAGTAACTCAGCTACATGATAAAAACAAAACAATTTCTTCTGAAGCG
  CTGGATATTCTTGATGAAGCTTGTGAAGACAAGGCCAATCTTCATGCTCTTATTCAGATGAAGCCAGCCT
  TATCCCACCTTGGAGACAAGGGCTTGCTTCTACTGCTGAGATTTCTTTCCATTCCGAAAGGATTTTCTTA
  CCTGAATGAAAGGGGTTATGTTGCAAAACAGTTGGAAAAGTGGCACAAGGAATATAATTCAAAGTATGTT
  GACTTGATTGAGGAACAACTTAATGAAGCCCTCACTACATACAGGAAGCCTATTGATGGTGATAACTACG
  TTCGTCGCAGTAATCAAAGACTACAGCGACCTCATGTCTACCTGCCCGTACACCTTTATGGACAACTGGT
  ACACCACAAAACAGGCTGCCATTTACTGGAAGTACAGAGTATCATCACAGAGCTCTGTCACAATGTTCGC
  ACTCCAGACTTGGACAAATGGGAAGACATTAAGAAGCTGAAAGCCTCTCTTTGGGCCTTGGGAAATATTG
  GCTCATCGAATTGGGGGCTTAATTTGCTACAGGAAGAAAATGTGATCCCAGATATACTAAAACTTGCAAA
  ACAGTGTGAGGTCCTTTCCATCCGAGGGACCTGTGTATATGTTCTTGGGCTCATAGCTAAAACCAAGCAA
  GGCTGTGATATTTTGAAATGTCACAGCTGGGATTCTGTAAGGCACAGTCGCAAGCATCTATGGCCAGTGG
  TTCCAGATGATGTGGAGCAGCTCTGCAATGAGCTCTCATCTGTCCCAAGCACCCTCAGCTTGAACTCTGA
  GTCGACCAGCTCTAGACATAACAGTGAGAGTGAGTCTGCACCATCAAGCATGTTCATGCTGGAGGACGAC
  CGCTTCGGCAGCACCTCCACCAGCACATTTTTCCTTGACATCAATGAAGATGCTGAGCCAGCATTTTATG
  ACCGACCTGGACCCATAAAAGATAAAAATTCATTTCCCTTTTTTGGCTCCAGCAAACTTGTAAAGAATCG
  TATCTTAAATTCACTTACTTTGCCTACTAAAAAACACCGCAGTAGCAGTGATCCAAAAGGAGGAAAACTG
  TCATCTGAAAATAAGACAAGCAACCGGCGAATCAGAACACTTACGGAGCCCAGTGTTGACTTGAATCACA
  GCGAAGACTTCACAAGCTCCTCAGCACAGAAATCATTACAGCTAGAGCCTTCCTTCGTTGGGAATAAGCA
  CCTTGAAGACGCGGGCAGCACTCCAAGTATTGGAGAGAATGACCTCAAATTCCCCAAGAGTTTTGGTACC
  GAGACTCACAGAGAAAACACAAGCCGAGAAAGGTTGGTTGTGGAAGGCTCAGCAAGCTCCCACATCAAGA
  TCCGCAGCCAGAGCTTTAACACGGACACTACGACGAGTGGCATCAGCTCCATGAGCTCGAGCCCCTCCCG
  AGAGACAGTCGCTGTCGACCCCACAGCTATGGACACGGACTGTGGAAGTCTGAGTACCGTGGTAAGTACT
  AAAACTGTTAAGACGAGCCACTATTTGACACCACAGTCTAACCATCTGTCTCTCTCCAAATCGAACTCAG
  TGTCCCTGGTGCCACCAGGTTCTTCTCACACCCTTCCTAGAAGAGCCCAGTCCCTTAAAGCGCCCTCCAT
  TGCAACAATAAAAAGTCTAGCAGATTGCAACTTCAGCTACACAAGCTCTAGAGATGCCTTTGGCTATGCT
  ACATTGAAAAGGTTACAGCAGCAAAGAATGCACCCGTCCTTGTCTCACTCGGAAGCTTTGGCGTCGCCGG
  CAAAAGATGTGCTTTTTACCGACACCATCACCATGAAGGCCAACAGCTTTGAATCCAGACTGACGCCAAG
  CAGGTTTATGAAAGCTTTAAGTTATGCGTCATTAGATAAAGAAGATTTACTAAGTCCTATTAATCACAAT
  ACCCTCCAGCGCTCCTCCTCAGTTAGGTCTATGGTGTCCAGTGCTACATATGGAGGTTCCGATGACTACA
  TTGGACTTGCTCTTCCAGTAGACATCAATGACATCTTCCAGATAAAGGATGTTCCTTATTTTCAATCAAA
  GCACGTGCCTCCACCAGATGACCGAGGTGCAAGAATGTTTTCCCATGATGGAGCAGGTCTTTCATCTGGA
  GCCGGTGGTCTCGTAAAGAACTCTTTTCACTTGCTGCGGCAGCAGATGAGTCTCACAGAAATAATGAACT
  CTGTCCATTCGGACGCCTCCCTGTTCTTAGAGAGCACAGAAGACACTGGGCTGCAGGAGCACACGGATGA
  CAATTGCCTTTACTGCGTCTGCATTGAACTCCTGGGCTTCCAGCCCAGTAACCAGCTAAGTTCCATCTGC
  AGTCACTCCGATCTTCAAGATATTCCGTACTCCGATTGGTGCGAGCAGACGATCCACAACCCCTTAGAAG
  TGGTTCCCTCTAAGTTCTCGGGGATTTCTGGCTGCAGCGATGGCGCATCTCAGGAAGAAGGCTCAGCCAG
  CAGCACCAAAAGCACTGAGCTGTTACTGGGTGTTAAAACCATTCCAGATGACACACCCATGTGCCGGATC
  CTGCTTCGTAAGGAAGTGCTAAGATTAGTTGTCAATTTGAGTAGTTCAGTTTCCACCAAGTGTCACGAGA
  CCGGGCTTCTGACCATTAAAGAGAAGTATCCTCAAACATTTGATGACATATGCCTTTATTCTGAAGTTTC
  TCATCTGTTGTCACACTGCACATTTAGACTTCAGTGTCGGCGGTTCATACAAGAGTTATTTCAGGATGTA
  CAGTTTTTACAAATGCATGAAGAAGCAGAGGCTGTGTTGGCAATTCCACCAATTCAGCCTATAGTTGATG
  AGTCTGCTGAATCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030168
• Insert Size: 5127 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030168.3, NP_084444.3
• RefSeq Size: 9328 bp
• RefSeq ORF: 5127 bp
• Locus ID: 78757
• UniProt ID: Q6QI06
• Cytogenetics: 15 A1
• Gene Summary: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.[UniProtKB/Swiss-Prot Function]