Cul7 (NM_025611) Mouse Untagged Clone

CAT#: MC224832

Cul7 (untagged) - Mouse cullin 7 (Cul7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cul7
• Synonyms: 2510004L20Rik; AA409809; C230011P08Rik; p185; p193
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224832 representing NM_025611
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGGGGAGCTACGATACAGGGAATTCAGGGTGCCCCTGGGGCCTGGCTTGCACGCGTATCCGGATG
  AATTGATCCGCCAACGGGTTGGCCATAATGGGCACCCCGAGTATCAGATCCGCTGGCTCATCCTCAGGCG
  CGGGGATGATGGGGACCGGGACTCTACAGTGGACTGCAAGGCTGAGCATATCCTGTTATGGATGTCTGAC
  GATGAGATCTATGCCAACTGCCACAAGATGCTGGGCGAGAATGGCCAAGTCATCGCACCTTCCCGGGAGT
  CCACTGAGGCAGGGGCCCTCGACAAGTCTGTGCTGGGGGAGATGGAAACAGATGTGAAGTCCTTGATTCA
  GAGGGCCCTTCGGCAGCTGGAGGAGTGCGTGGGCACCGTGCCTCCTGCCCCTCTCCTTCACACGGTCCAT
  GTACTCAGTGCCTATGCCAGCATCGAGCCCCTCACTGGCATCTTCAAAGACCGCAGGGTTGTGAACTTGC
  TCATGCACATGTTGAGCAGTCCTGATTATCAGATCCGCTGGAGCGCAGGCCGGATGATCCAAGCTCTGTC
  CTCCCACGATGCTGGGACCCGGACCCAGATCCTTCTGTCATTGAGCCAACAAGAGGCCATTGAAAAGCAC
  CTGGATTTTGATAGCCGCTGCGCTCTGCTTGCACTGTTCGCCCAGGCTACTCTCACGGAACACCCGATGT
  CTTTCGAGGGCGTTCAGCTGCCACAGGTCCCAGGACGGCTGCTCTTCTCCCTGGTAAAACGCTACCTGCA
  CGTCACCTTCCTCCTGGATCGGCTGAACGGCGATGCAGGGGATCAAGGAGCCCAGAACAACTTTATTCCT
  GAGGAGTTGAATGTAGGGAGGGGCCGGCTGGAACTGGAATTCAGTATGGCCATGGGCACTCTGATCTCTG
  AGCTGGTGCAGGCCATGCGCTGGGACGGGGCCTCAAGCAGACCAGAGAGTTCTTCCTCCTCCACCTTCCA
  GCCTCGGCCAGCACAGTTCCGCCCCTACACCCAGCGTTTCAGGAGGTCGAGGCGGTTTCGCCCCCGTGCC
  TCGTTTGCCAGTTTTAATACCTATGCCTTGTATGTGCGGGACACGCTGCGGCCCGGGATGCGGGTACGGA
  TGCTGGAGAATTACGAGGAGATCGCTGCTGGGGATGAGGGCCAGTTCCGACAGAGCAATGATGGCGTTCC
  CCCAGCGCAGGTGTTGTGGGATTCAACAGGCCATACCTACTGGGTGCACTGGCACATGCTGGAGATCTTG
  GGCTTTGAGGAAGACATCGAGGATGTGATTGATATTGAAGAGTTACAGGAGCTAGGGGCCAATGGAGCAC
  TGAGCATCGTCCCGCCGTCCCAGCGCTGGAAGCCCATAACTCAGCTCTTTGCCGAGCCTTACGTGGTACC
  CGAGGAGGAAGACAGGGAAGAGAGCGAGAACTTGACCCAGGCTGAGTGGTGGGAGCTCCTCTTCTTCATC
  CGGCAGTTGAGTGAGGCAGAGCGCCTTCACATCGTGGATCTCCTGCAAGACCACCTGGAAGAGGAGCGCG
  TTCTGGACTACGATATGCTGCCTGAGCTGACCGTGCCCGTTGACTTGGCCCAGGATCTGCTGTTGTCTCT
  GCCTCAGCAACTTGAGGACAGTGCTCTGAGGGACCTGTTCAGCTGCAGTGTCTACAGGAAGTATGGGCCC
  GAAGTCCTGGTAGGGCATCTAAGCTACCCATTTGTGCCAGGTGCCCAGCCAAATTTATTCGGAGCCAATG
  AAGAGTCTGAAGCCAAAGATCCCCCACTTCAGAGTGCCAGCCCTGCCCTGCAGCGCCTGGTGGAGAGCTT
  GGGCCCCGAAGGGGAGGTCCTTGTGGAACTGGAACAAGCCCTCGGCTCCGAGGCTCCCCAGGAAACTGAG
  GTCAAGTCCTGCTTGTTGCAGCTCCAGGAGCAGCCCCAGCCCTTCCTCGCTCTGATGCGGAGCCTGGACA
  CTTCCGCCAGCAACAAGACCCTGCACCTCACTGTGCTCAGAATCTTAATGCAGCTGGTGAACTTCCCAGA
  GGCGCTGTTGCTACCCTGGCACGAGGCCATGGATGCCTGCGTGACCTGCCTTCGGTCCCCCAATACTGAC
  CGAGAGGTGCTCCAGGAACTAATCTTTTTCCTGCACCGCCTGACCACCACAAGCCGGGACTATGCGGTGA
  TACTAAACCAGCTAGGAGCCCGGGACGCCATCTCCAAAGTCCTGGAAAAGCACCGAGGGAAACTGGAGTT
  GGCTCAGGAGCTGCGGGATATGGTGTCCAAGTGTGAGAAGCATGCCCACCTCTACCGGAAACTCACCACC
  AACATCCTGGGCGGTTGCATCCAGATGGTCCTGGGTCAGATTGAAGACCACAGACGAACCCACCGGCCCA
  TCCAAATCCCATTCTTTGATGTGTTTCTCAGATATCTGTGCCAGGGCTCCAGTGAGGAAATGAAGAAAAA
  CAGGTACTGGGAGAAGGTGGAGGTGTCCTCCAACCCACAGCGGGCCAGCAGGCTGACGGACCGCAACCCC
  AAGACCTACTGGGAGTCCAGTGGCAGGGCCGGCTCCCACTTCATCACCTTACACATGCGCCCAGGTGTCA
  TCATCAGGCAGCTGACTCTACTGGTGGCTGGCGAGGACTCAAGCTACATGCCAGCCTGGGTGGTGGTATG
  CGGGGGCAACAGCATCAAGTCCGTTAATAAAGAACTCAACACGGTAAACGTGATGCCCTCTGCCAGCCGG
  GTGACCCTCCTGGAGAACCTGACCCGCTTCTGGCCCATCATCCAAATCAGAATAAAGCGCTGCCAGCAGG
  GTGGCATTAACACGCGCATCCGGGGCCTAGAGGTGCTGGGCCCCAAGCCCACCTTCTGGCCAGTGTTCCG
  AGAGCAACTGTGCCGGCACACGCGCCTCTTCTACATGGTTCGGGCCCAGGCATGGAGTCAGGACATAGCA
  GAGGACCGCCGGAGCCTTCTGCACCTGAGTTCTAGGCTAAATGGGGCTCTGCGCCATGAACAGAATTTTG
  CAGAGCGCTTCCTTCCTGATATGGAGGCCGCCCAAGCACTGAGCAAGACCTGCTGGGAGGCGCTGGTCAG
  CCCCCTGGTGCAGAACATTACATCTCCCGATGAGGACAGCACCAGCTCCTTGGGCTGGCTGCTGGATCAG
  TACTTGGGATGCAGGGAGGCTGCCTACAATCCCCAGAGCAGGGCTGCTGCTTTCTCCTCCCGGGTTCGCC
  GCCTTACCCACCTCCTGGTCCATGTGGAGCCCCGTGAGGCAGCACCTCCGGTGGTGGCCATCCCTCGATC
  CAAGGGCAGGAACAGAATCCATGACTGGAGCTACTTGATCACCCGGGGCCTTCCAAGTAGCATCATGAAG
  AACCTGACCCGCTGTTGGCGGTCTGTGGTGGAGGAGCAGATGAACAAGTTTCTGACTGCGTCCTGGAAAG
  ACGATGATTTCGTACCCCGCTATTGCGAGCGCTATTACGTCCTGCAGAAGTCCAGCTCAGAGCTGTTTGG
  GCCACGAGCTGCCTTCTTGCTGGCAATGCGGAATGGCTGTGCTGATGCTGTGCTGAGGCTCCCTTTCCTC
  AGGGCCGCCCATGTGAGCGAGCAGTTTGCTCGGCACATTGACCAGAGGATCCAAGGCAGTAGGATGGGTG
  GAGCCCGGGGAATGGAGATGCTGGCACAGTTGCAGCGATGCCTGGAGTCTGTCCTGATTTTCTCTCCCCT
  GGAGATAGCCACCACCTTTGAGCATTACTACCAGCACTACATGGCTGACCGTCTCCTGAGCGTAGGCTCC
  AGCTGGCTGGAGGGGGCCGTACTGGAGCAGATCGGTCCCTGCTTCCCCAGCCGTCTTCCCCAGCAGATGC
  TACAGAGCCTGAACGTCTCAGAGGAGTTGCAGCGTCAGTTCCACGTTTACCAGCTGCAGCAGCTTGATCA
  GGAGCTCCTGAAGCTGGAAGACACGGAAAAGAAGATACAGGTGGCCCATGAGGACAGTGGCAGAGAGGAC
  AAGAGCAAGAAGGAAGAAGCCATTGGAGAAGCCGCGGCTGTGGCTATGGCAGAGGAGGAGGATCAAGGGA
  AGAAGGAGGAGGGAGAGGAGGAAGGGGAGGGAGAGGATGAGGAGGAAGAGCGCTATTATAAAGGAACAAT
  GCCAGAAGTGTGTGTACTTGTCGTGACGCCACGCTTCTGGCCTGTCGCCTCCGTCTGCCAAATGCTCAAC
  CCGGCAACGTGCCTGCCCGCATACCTGCGGGGGACCATAAACCACTACACCAACTTTTACAGCAAGAGTC
  AGAGCCGCTCCAGCTTAGAGAAAGAGCCACAGAGGCGGCTGCAGTGGACCTGGCAGGGCCGGGCAGAAGT
  GCAGTTCGGGGGTCAGATTCTGCATGTGTCCACAGTACAGATGTGGCTGCTGCTGCATCTCAACAACCAA
  AAGGAGGTGTCTGTCGAGAGCCTGCAGGCTATCTCGGAGCTCCCTCCAGATGTGCTTCACAGGGCCATCG
  GGCCTCTCACCTCATCAAGAGGTCCCTTGGACCTGCAGGAGCAGAAGAACGTACCAGGAGGGGTGCTCAA
  GATTCGAGATGACAGTGAGGAACCCAGGCCGAGGAGGGGCAACGTGTGGCTGATCCCACCTCAGACATAC
  CTACAAGCTGAGGCCGAAGAGGGCCGGAACATGGAGAAGAGAAGGAATCTTCTGAATTGCCTTGTTGTCC
  GAATCCTCAAGGCTCACGGGGATGAAGGCTTGCATGTTGACCGGCTCGTCTATCTGGTGCTAGAAGCGTG
  GGAGAAAGGCCCGTGTCCTGCTAGGGGTCTGGTCAGCAGCCTCGGCAGGGGAGCAACCTGCAGGAGCTCT
  GATGTCCTCTCCTGCATCCTGCACCTCCTGGTCAAGGGCACGCTGAGACGCCATGACGACCGGCCGCAGG
  TGCTGTACTATGCAGTTCCTGTAACTGTGATGGAGCCCCACATGGAGTCCCTGAACCCTGGCTCGGCAGG
  CCCCAATCCACCCCTCACCTTCCACACCCTGCAGATTCGATCCCGGGGTGTGCCTTACGCCTCCTGCACT
  GATAACCACACCTTCTCCACTTTCCGGTAG
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_025611
• Insert Size: 5070 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025611.5, NP_079887.3
• RefSeq Size: 5546 bp
• RefSeq ORF: 5070 bp
• Locus ID: 66515
• UniProt ID: Q8VE73
• Cytogenetics: 17 C
• Gene Summary: Core component of the 3M and Cul7-RING(FBXW8) complexes, which mediates the ubiquitination of target proteins. Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer. Interaction with CUL9 is required to inhibit CUL9 activity and ubiquitination of BIRC5. Core component of a Cul7-RING ubiquitin-protein ligase with FBXW8, which mediates ubiquitination and consequent degradation of target proteins such as GORASP1, IRS1 and MAP4K1/HPK1. Ubiquitination of GORASP1 regulates Golgi morphogenesis and dendrite patterning in brain. Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation. Acts as a regulator in trophoblast cell epithelial-mesenchymal transition and placental development. Does not promote polyubiquitination and proteasomal degradation of p53/TP53. While the Cul7-RING(FBXW8) and the 3M complexes are associated and involved in common processes, CUL7 and the Cul7-RING(FBXW8) complex may be have additional functions (By similarity). Probably plays a role in the degradation of proteins involved in endothelial proliferation and/or differentiation.[UniProtKB/Swiss-Prot Function]