Nrap (NM_198059) Mouse Untagged Clone

CAT#: MC224831

Nrap (untagged) - Mouse nebulin-related anchoring protein (Nrap), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nrap
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224831 representing NM_198059
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGTGCAGGCCTGCTCTAGGTGTGGCTATGGGGTTTACCCTGCTGAGAAGATCAGCTGCATAGATC
  AGACATGGCACAAAGCATGTTTTCACTGTGAAGTCTGTAAGATGATGCTTTCTGTTAATAACTTCGTGAG
  TCACCAGAAAAAGCCGTACTGTCACGCCCACAACCCTAAGAACAACACGTTCACTAGCGTCTACCACACA
  CCCTTAAATCTAACCTTGAAGAAGTCTGTGGCAGCCATGGGAGGGATTGACGGCAAAGAAGATGGCGAGC
  CATTTAAATCCGTTCTCCATTGGGACATGAAGTCCAAAGCTGGAGCAGGGGCAGCTAGCCGGCTGATGAA
  TGAGAGAGACTACTGGCCAGGGTATGCAGAAGGGAACACTTGGTGTCCAGGAGCTCTGCCGGACCCTGAG
  ATTGTAAGGATGGTTGAAGCTCGACAGTCTCTCGGTGAGGGGTACACAGAAGACCGTGAACAGCAGCAGG
  GCAAGGGGAGCTTTCCAGCCATGATCACCCCTGCCTACCAAAGGGCCAAGGCAGCCAACCAGCTGGCCAG
  CCAGGTACAATACAAGAGAGGCCACGATGAGCGGGTCTCCACATTCACTCCAGTGGCGGACACGCCCGAG
  CTGCTCCGTGCCAAAGCTGGGGGACAGCTTCAAAATGACGTGAGGTACACAGAAGACGGTGGGCAGCAGA
  GAGGGAAAGGCAGTTTTCCTGCTATGATCACACCAGCTTATCAGATAGCCAAAAGGGCCACTGAGCTGGC
  GAGTGATGTGAGGTACCATCAACAATATCATAGAGAAATGAAGGGAATGGCTAGTCCTGTCGGAGCCGAG
  GGTGGGATGACAAAGGACTCTGTAGACCGATGTGGCCAGGTTTACTCAGAAGAGTGTGATGAACCCAGAG
  GAAAGGGCAGCTTCCCGGCCATGATCACCCCAGCGTATCAGAACGCCAAGAAGGCCAATGAACTTGTTAG
  TGACATAAAATACCGGCAGGACTTCCATAAGATGAAAGGTGCAGCGCATTTTCACTCCCTTGCAGCCCAG
  GACAACTTGGTTCTTAAGCGAGCTCAGAGTGTGAGCAAGCTTGTGAGTGAGAATAAATATAAAGAAAACT
  ATCAGACCCAGCTGAGAGGCCACTATGACGGAGTCGGCATGGACAGACGCATGCTCCACGCTCTCAAAGT
  TGGCAGCCTGGCTAGCAATGTTGCCTACAAGGCTGATTATAAACATGACGTTGTGGATTACAACTACCTG
  GCCACTGCCACTCCATTCTACCAAACAACGATGAGGCTGGTTCCCCTGAAGGATGTCAACTACAGGCAGA
  ACATTGACAGAATGAAGTTCAGCTCGGTGACAAACACGCCACAGATTGTTCAAGCTAAGATCAACGCCCA
  GCAGCTGAGCCATGTGAATTACCGAGCTGACTACGAGAGAAACAAGCTGAACTACACATTGCCCCAGGAC
  GCCCCTCAGCTGCTGAAGGCCAAGGCCAATGCAGAACTGTTCAGCGAGGTGAAGTACAAAGAAGGCTGGC
  AGAAGACAAAGGGGAAAGGCTTTGAGATGAAGCTGGACGCCATGTCTCTGTTGGCCGCCAAAGCCTCGGG
  GGAGCTTGCTAGCAGCGTTAAATACAAAGAAGAATATGAGAAAATGAAGGGCAGAGCCCTGGGAGCCACA
  GACTCTAAGCTTCTGCATTCTCTGCAGGTGGCCAAGATGAGCAGCGAGGTTGAATATAAGAAAGGGTTTG
  AGGAGAGTAAAACCCACTTTAACCTGCCCATGGACATGGTGAACCTCAGGCACGCTAAGAAGGCCCAAGC
  TCTTGCCAGTGACCTTGACTACAGAAAGAAACTGCATGACTACACGGTGCTGCCTGAAGATATGAAAACT
  CAGTGGGCCAAGAAAGCCTACGGGCTGCAGAGCGAGCTGCAGTACAAAGCTGACCTGGCATGGATGAGGG
  GAGTCGGGTGGCTGACAGAGGGGAGTCTCAACCTGGAACAGGCGAAGAAGGCTGGACAGCTCATCAGCGA
  GAAAAACTACCGGCAGAGGGTGGATGAGCTGAAGTTCACCAGTGTGGCTGACAGCTCCCAGATGGAGCAT
  GCCAAGAAGAGCCAGGGGCTGCAGAACGCGGTGGCCTACAAGGCAGGAAACGAGCAATCCGTCCATCAGT
  ACACCATCAGCAAAGACGAGCCTCTCTTCTTACGAGCCCGGGCCAATGCTGCTCAACTCAGCGAGACCCT
  GTACAAGAGCAGCTGGGAAAAGCAGAAGGCCAAAGGGTTTGAGCTGAGACTTGACTCCTTGGCATTCCTG
  ACAGCCAAAGCCAAGAGAGACCTGGCTAGTGAGGTGAAATACAAAGAGGACTATGAAAGGTCCAGAGGGA
  AACTCATTGGGGCGAAGAGTGCACAGGGGGATTCGCAGATGAGCCACTCCCTGCAGATGTCGAAGCTACA
  GAGTGACCTGGAGTACAAGAAGGGCTTTGAAGACACCAGATCTCAGTGCCACATCTCCCTTGACATGGTT
  CACCTCGTCCATGCTCGCCAGGCACAGCACCTAGCCACAGACGTAGGCTACAGGACAGCGTCTCACTGCT
  TCACGGCTCTGCCCACAGACATGAAGGTGGAGTGGGCCAAGAAGGCTTACGGCCTGCAGAGTGATAACCA
  ATACAGGGCAGACATGAAGTGGATGAAAGGCACAGGCTGGGTGGCCACCGGGTCATTACATGTGGAGCAG
  GCGAAGAAGGCAGGAGAACTCATTAGTGAGAAGAAGTACCGTCAGCACCCAGATGCTTTGAAGTTTACCA
  GTATTAAAGACACTCCGGAGATGGTCCAGGCCAGGATTAGTTATACCCAAGCAGTGGATAGACTGTACAG
  GGAGCAAGGGGAGAATGTGAAGCACCACTACACACAGACTGCAGACTTGCCTGAAGTCCTGCTGGCCAAA
  CTGAACGCCATGAACATCAGCGAGACTCGCTATAAGGAATCTTGGAGCCGGCTTCGAGATGGAGGATACA
  AGCTGAGGCTGGATGCCCTTCCATTCCAGGCAGCAAAGGCGTCCAGCGAAGTTATAAGTGACTACAAATA
  CAAAGAAGCATTTGAGAGAATGAAAGGCCAGATGCTCGGCTCCCGGAGCTTGGAAGATGACCTCAGCCTT
  GCTCATTCAGTGCACGCGACCTCGCTGCAGAGTGATGTGAATTATAAGAAAGGCTTTGAACACGCAAAGG
  CTCACTTCCATCTGCCGCTGGATATGGTGACCTTGGTGCATGCCAAGAAGGCTCAAACCCTGGCCAGCGA
  CCAGGACTACAGACATCCGCTCCCCCAGCACACAGTCTTGGCAGAAGATCTGAGGCTGAGCTGTGCCAAG
  AAAGCTCACAAGTTACAGAGTGAGAATCTGTACCGCTCGGACTTGAACTTCATGCGTGGTGTTCCCTGTG
  TCGTTCCTGGAACACTGGAGATTGAAGGAAGAAAGAAAGCCTCTGAGCTCATCAGCGAGAGTAAATACCG
  CCAGCATCCTGGGTCATTCAAGTACACGGCCGTGACAGATACTCCCAACCTCCTGCATGCCAAGTACAGC
  AACCAGATCACCAATGAGCGTCTCTATAAAGCGGCTGGCGAGGACGCACGGCACCAATACACGATGACCC
  TTGGTCTGCCTGAGTTCATCCGAGCCAAAACCAACGCAGCCAACCTGAGCGAGGCAAAATACAAGGAGGC
  TTGGCATAATCTTCGTGCTCAAGGCTACAAGCTGACGATAGACGCCCTCCCCTTCCAGGCTGCTCGGGCC
  TCTGGAGACATAGCCAGTGACTTTCTCTACAGGCACGAGTTTGTGAAGGAGCGTGGACAGCTCATTGGGG
  TGCGGAATGTGAGTGACGACCCGCGGCTACTGCACTGCCTACGGATGGGCCAGCTGCAGAGTGAGAATCA
  GTACAGGAAGGAGGCGGCCAGCAGCCAAGCTCAGTGCCACCTGCCCATGGATATGATGTACCTGGTCCAT
  GCCAGGAAGGCCCAGGCCCTGGCCAGTGACCACGACTACCGGACACAGTGCCACGAGTTCACCGCCCTGC
  CTGAGGATCTGAAGATGGCCTGGGCCAAGAAAGCGCACGCCCTGCAGAGTGAGTTTCGCTACAAGGCGGA
  CTTGATGGGCATGAAAGGGACAGGATGGCTGGCTCTGCAGTCTCCTCAGATAGAGAGTGCAAAGAAGGCT
  GGAGACCTAATCAGTGAGACCAAGTACCGTAAAAAACCAGACAGCATCAAGTTCACTACCGTGGTTGACT
  CTCCAGACCTCATTCATGCCAAGGAGAGCTACATGCACTGTAACGAGCGCTTGTACAGGTTGGGAGATGC
  AGCCTCCCTGCATAGATACACCCCAATCCCAGACCACCCGGATTTCACCCGAGCCCGCATGAACGCAATG
  CATTTGAGTGACAAAGTCTACAGGAATGCCTGGGAGCAGAGCCGGGCTGGTGGCTATGACTTCAGGCTGG
  ATGCTATCCCATTCCAGACTGCACGGGTGTCCAGGGATATCGCCAGTGATTTCCGGTACAAAGAGGCATT
  TCTGAGGGACCGGGGCCTACAGATTGGGTACCGTAGCATCAGTGACGACCCGAGGACGACGCACTTCCTC
  CGAGTCGGCAGGCTCCAGAGTGACAACGAGTACAGGAAGGCCTTTGCCAAAGGTCGCTCCCAGTTCCACA
  GTCGCGCTGACCAACCAGGCTTCCTCCAGGCCAAGAGGAGCCAGCAGCTGGCCAGCGATGTCCTCTATAG
  GCAGCCACTGCCTCAGCACACCAGCGACCCAGAACAGCTGGGCCTGAAGCATGCCCGGAAAGCCCATCAG
  CTGCAGAGTGATGTCAAGTACAAATCTGACTTGAACCTGACTCGAGGTGTTGGCTGGACCCCTCCTGGCT
  CCTACAAAGTGGAAATGGCTCGACGGGCTGCAGAACTGGCCAACAGGAGGGGCCCGGGGATCCGGGGGGC
  TTCTGTGGAGCCAGAGGCAGCAGCGGCGCTAGGAGATCATCAGAGCAGGGGGGTGAACCCGGATGCCTCG
  GAGATCCTGCATATCCACAAGAAGAAGACTCTGCTGATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_198059
• Insert Size: 5082 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198059.3, NP_932307.2
• RefSeq Size: 5397 bp
• RefSeq ORF: 5082 bp
• Locus ID: 18175
• UniProt ID: Q80XB4
• Cytogenetics: 19 51.8 cM
• Gene Summary: May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) lacks an in-frame exon in the central coding region, compared to variant 2. The encoded isoform (C) is shorter, compared to isoform S.