Trpm1 (NM_001039104) Mouse Untagged Clone

CAT#: MC224766

Trpm1 (untagged) - Mouse transient receptor potential cation channel, subfamily M, member 1 (Trpm1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trpm1
• Synonyms: 4732499L03Rik; AI606771; Ltrpc1; Mlsn1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224766 representing NM_001039104
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTCCATGAGGAAGATGAGCAGCTCCTTCAAGCGTGGTTCAATCAAGAGCTCCACATCAGGGTCCC
  AGAAGGGTCAGAAAGCATGGATAGAGAAGACATTTTGCAAAAGGGAATGCATCTTTGTAATTCCCAGTAC
  AAAAGACCCTAACAGATGTTGCTGTGGTCAGCTCACTAACCAGCACATCCCCCCTCTGCCGAGTGGGGCT
  CCCAGCACAACAGGAGAGGACACCAAGCAGGCAGACACGCAGTCCGGGAAATGGTCTGTCAGCAAACACA
  CCCAGAGCTACCCAACAGACTCCTATGGGATTCTTGAATTCCAGGGTGGGGGTTACTCCAATAAAGCCAT
  GTACATCCGAGTCTCCTACGACACCAAGCCAGATTCCCTGCTCCACCTCATGGTGAAGGACTGGCAGCTG
  GAGCTCCCGAAGCTCTTGATATCTGTGCACGGAGGCCTCCAAAGCTTCGAGATGCAGCCCAAACTGAAGC
  AGGTGTTTGGGAAAGGTCTGATCAAGGCTGCCATGACCACGGGGGCGTGGATCTTCACCGGGGGTGTGAG
  CACTGGTGTCGTCAGCCATGTGGGGGATGCCTTGAAAGACCACTCCTCCAAGTCCAGAGGCCGGCTCTGT
  GCTATAGGAATTGCTCCCTGGGGCATGGTGGAGAACAAGGAAGACCTGATTGGAAAAGATGTAACAAGAG
  TCTATCAGACCATGTCCAACCCTCTGAGCAAGCTCTCTGTGCTCAACAATTCCCACACTCACTTCATCTT
  GGCTGACAACGGCACCCTGGGCAAGTATGGTGCTGAGGTGAAGCTTCGAAGACAGCTGGAAAAACACATC
  TCCCTGCAGAAGATCAACACAAGGCTGGGCCAGGGTGTACCTGTCGTGGGCCTAGTGGTAGAAGGTGGTC
  CTAACGTGGTTTCTATCGTCCTGGAGTATCTCAAAGAAGACCCTCCTGTCCCTGTGGTGGTTTGCGATGG
  CAGTGGACGTGCCTCTGACATTTTGTCCTTCGCACACAAATACTGCGACGAAGGAGGAGTCATAAACGAG
  TCCCTGCGGGACCAGCTTCTAGTTACCATTCAGAAAACATTTAATTACAGCAAGTCCCAGTCGTATCAGC
  TGTTTGCAATTATCATGGAGTGCATGAAGAAGAAAGAACTCGTCACTGTGTTTCGGATGGGTTCCGAGGG
  TCAGCAAGATGTCGAGATGGCAATTTTAACTGCCTTGCTCAAAGGAACCAACGCATCAGCTCCAGATCAG
  CTGAGCTTGGCCCTGGCTTGGAACCGGGTCGACATAGCGCGAAGCCAGATCTTCGTCTTTGGCCCACACT
  GGCCGCCACTGGGAAGCCTGGCCCCTCCTGTGGACACCAAAGCCACAGAGAAGGAAAAGAAGCCACCCAC
  AGCCACCACCAAGGGGAGAGGAAAAGGAAAAGGCAAGAAGAAAGGCAAAGTGAAAGAGGAAGTGGAGGAA
  GAGACGGACCCCCGGAAGCTTGAGCTGCTCAACTGGGTGAATGCCCTGGAGCAAGCCATGCTGGATGCTC
  TTGTCCTAGATCGGGTGGACTTTGTAAAGCTCCTGATTGAAAACGGAGTGAACATGCAGCATTTCCTCAC
  CATCCCGAGGCTGGAGGAGCTATACAACACCAGACTGGGCCCACCAAACACCCTTCATCTGCTGGTGCGG
  GATGTAAAGAAGAGCAACCTTCCACCTGATTACCACATCAGCCTCATTGATATAGGACTGGTGCTGGAGT
  ACCTCATGGGCGGTGCCTACCGCTGCAACTACACTCGGAAAAGCTTCCGGACTCTCTACAACAACTTGTT
  TGGCCCTAAGAGGCCGAAAGCACTTAAGCTTCTGGGAATGGAAGATGATGAACCCCCAGCCAAAGGCAAG
  AAGAAAAAGAAGAAGAAGAAAGAGGAAGAGATTGACATCGACGTGGACGACCCCGCCGTGAGTAGGTTCC
  AGTACCCGTTCCATGAGCTCATGGTATGGGCCGTGCTCATGAAGCGCCAGAAGATGGCGGTCTTCCTGTG
  GCAGCGTGGGGAAGAGTGCATGGCGAAGGCCTTGGTGGCCTGCAAGCTCTACAAGGCCATGGCCCACGAG
  TCCTCCGAGAGCGAGCTGGTGGATGACATCTCCCAGGATCTGGACAACAATTCCAAAGATTTTGGCCAGC
  TCGCTGTGGAGCTGTTAGACCAGTCTTACAAGCACGATGAGCAGGTTGCCATGAAACTTCTGACCTACGA
  GCTCAAGAACTGGAGCAACTCAACCTGCCTGAAGCTGGCTGTGGCAGCCAAGCACCGGGACTTCATTGCT
  CACACCTGCAGCCAGATGCTGCTGACAGACATGTGGATGGGAAGGCTTCGCATGAGGAAGAACCCTGGCC
  TGAAGGTCATCATGGGGATCCTTATCCCACCCACCATCTTGTTTTTGGAATTCCGTACATATGATGACTT
  CTCCTATCAAACATCCAAGGAGAACGAAGATGGCAAAGAAAAGGAAGAAGAGAATGTGGATGCAAATGCA
  GATGCTGGCTCGAGAAAGGGGGATGAGGAGAATGAGCACAAAAAGCAGAGGAGCATTCCCATTGGAACAA
  AGATCTGTGAATTCTATAATGCGCCCATTGTCAAGTTCTGGTTTTACACAATTTCCTACTTGGGCTACTT
  GCTGCTGTTCAATTATGTCATCCTGGTGCGGATGGATGGATGGCCATCACCCCAAGAATGGATTGTCATC
  TCCTACATTGTGAGCCTTGCATTGGAGAAAATACGAGAGATCCTCATGTCGGAACCAGGCAAACTCAGCC
  AGAAAATCAAAGTGTGGCTCCAGGAGTACTGGAACATCACAGACCTCGTGGCCATCTCCATGTTCATGGT
  TGGGGCCATCCTTCGTCTCCAGAGCCAGCCATACATGGGCTATGGTCGGGTCATCTACTGTGTGGATATC
  ATCCTCTGGTACATCCGAGTGTTAGACATCTTTGGTGTCAACAAATATCTTGGTCCCTATGTGATGATGA
  TTGGAAAGATGATGATTGACATGCTCTACTTTGTGGTCATTATGCTGGTTGTACTGATGAGTTTTGGAGT
  GGCTCGGCAAGCCATCTTGCATCCGGAGGAGAAGCCTTCCTGGAAACTGGCCCGAAACATCTTCTACATG
  CCATACTGGATGATCTACGGAGAGGTGTTTGCAGACCAGATAGACCTCTACGCCATGGAAATCAACCCTC
  CTTGTGGTGAGAATCTCTATGATGAGGAAGGCAAGAGGCTCCCTCCCTGCATCCCTGGTGCCTGGCTCAC
  ACCTGCCCTCATGGCCTGTTATCTCCTGGTGGCCAATATCCTGCTGGTCAACCTACTGATTGCTGTTTTC
  AACAATACCTTCTTTGAAGTCAAGTCAATATCCAATCAAGTGTGGAAGTTCCAGCGATACCAGCTGATCA
  TGACATTTCATGACAGGCCGGTCCTGCCCCCACCAATGATCATCTTAAGTCACATCTATATCATCATTAT
  GCGCCTCAGCGGTCGCTGCAGAAAAAAGAGAGAGGGGGACCAGGAGGAGCGGGACCGTGGGTTGAAGTTG
  TTCCTTAGCGACGAAGAACTGAAAAAACTGCATGAGTTTGAGGAGCAGTGCGTGCAGGAGCACTTCCGGG
  AAAAGGAGGATGAGCAGCAGTCGTCCAGCGACGAGCGCATCCGGGTCACAAGCGAGAGGGTTGAAAATAT
  GTCAATGAGATTGGAGGAAATCAACGAAAGAGAAAATTTCATGAAAACTTCTCTGCAGACTGTTGACCTT
  CGGCTCTCTCAGCTGGAGGAGCTATCTGGCAGGATGGTGAGCGCTCTGGAGAATCTGGCAGGCATCGACA
  GGTCTGATCTGATCCAGGCTCGATCTCGAGCATCCTCCGAATGCGAGGCCACCTACCTGCTGCGGCAGAG
  CAGCATCAACAGTGCCGACGGCTACAGCTTGTACCGCTACCATTTCAATGGCGAGGAGCTTCTGTTTGAG
  GAGCCTGCTCTCTCCACTTCGCCAGGGACAGCGTTCCGGAAAAAAACCTACTCCTTTCGTGTGAAGGATG
  AGGATGCAAAGTCGCACCTGGACCAGCCCAGCAACCTGCACCACACCCCTGGCCCCAGCCCACCTGCCAC
  GCCAGGCCGCAGCCGGCTCGCCCTTGAAGGTCCCTTGAGCACAGAGCTGAGACCTGGATCGGACCCTGGC
  ATCTCCGCAGGCGAGTTCGATCCGCGGGCGGATTTCAAGAGCACGGAAGCTGCACCAAGTCTGAACGCAG
  CGGGTGTTACAGGCACTCAACTGACTGTCGAAAGCACCGATTCCCACCCTCTGCGGGAGAGCAAACTCGT
  GCGCTACTACCCCGGAGACCCTAACACCTACAAAACAATGAAGTCCAGAAGCTTCGTCTATACTGAAGGA
  AGAAAGCTAGTCCGAGGTCTTAGCAACTGGAGTGCAGAGTACAGTTCAATCATGGACCAAGCATGGAACG
  CGACAGAATGGAGATGTCAAGTTCAAAGGATCACGCGCTCCCGCAGCACAGACATCCCATACATCGTGTC
  CGAAGCAGCCTCGCAAGACGAGCTTGAAGATGAGCACAGAGGATCTCTTCTGGATCCTCAGATCTCCCGT
  TCAGCCCTCACCGTCTCCGACAGGCCAGAAAAGGAAAACTTACTCTCTGTGAAGCCACATCAGACTTTAG
  GATTCCCCTGCCTACGGTCAAGAAGTTTACATGGCCGTCCTAGGAGTGCTGAGCCCGCTCCTAGCAAATT
  AGACAGGGCAGGACATGCCAGCAGCACTAGCAACTTAGCAGTTATGTCAGTTGTTCCAGAGGGACAAAAC
  ACCCAGCAAGAGAAAAGAAGCGCGGAAACTGAGTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001039104
• Insert Size: 4869 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039104.2, NP_001034193.2
• RefSeq Size: 5711 bp
• RefSeq ORF: 4869 bp
• Locus ID: 17364
• UniProt ID: Q2TV84
• Cytogenetics: 7 34.61 cM
• Gene Summary: Cation channel essential for the depolarizing photoresponse of retinal ON bipolar cells. It is part of the GRM6 signaling cascade. Calcium channel which may play a role in metastasis suppression. May act as a spontaneously active, calcium-permeable plasma membrane channel.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (TRPM1-L, PMID: 19966281, also known as Trpm1delta189-1078) differs in the 5' UTR and 5' coding region, and includes several additional exons at the 3' end, compared to variant 1. The resulting isoform (2) has distinct N- and C-termini, and is longer than isoform 1.