Vcan (NM_001134474) Mouse Untagged Clone

CAT#: MC224753

Vcan (untagged) - Mouse versican (Vcan), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vcan
• Synonyms: 5430420N07Rik; 9430051N09; Cspg2; DPEAAE; hdf; NG2; PG-M; PG-M(V0); PG-M(V1)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224753 representing NM_001134474
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGATAAATATGAAAGGCATCTTATGGATGTGCTCAACCTTATTACTAACCCATGCACTACATCAAG
  CCAAAATGGAAACCAGCCCACCTGTTAAAGGCTCTCTGTCTGGAAAAGTGGTCCTACCTTGTCATTTTTC
  AACCTTACCTACCTTACCACCCAATTACAACACGAGTGAATTTCTCAGAATCAAATGGTCTAAGATGGAA
  GTGGACAAAAATGGAAAAGATATAAAGGAGACGACTGTCTTGGTGGCCCAGAACGGAAATATCAAGATTG
  GTCAGGACTACAAGGGGCGAGTGTCCGTGCCTACACATCCCGATGATGTAGGTGATGCTTCCCTCACCAT
  GGTCAAACTCCGGGCTAGTGATGCAGGCGTCTACCGATGTGATGTCATGTATGGGATTGAAGACACTCAG
  GACACCATGTCACTGGCTGTGGATGGTGTTGTGTTTCACTACAGGGCAGCCACCAGCAGGTACACTCTGA
  ACTTTGCCGCTGCTCAACAGGCTTGTTTGGATATCGGGGCGGTCATAGCAAGCCCAGAGCAGCTGTTTGC
  CGCCTATGAGGATGGATTTGAGCAGTGTGATGCAGGATGGCTGTCTGATCAAACTGTCAGATATCCCATA
  CGGGCTCCCCGAGAGGGCTGTTACGGAGACATGATGGGGAAGGAAGGGGTTCGGACCTATGGATTCCGCT
  CTCCCCAGGAAACCTATGATGTGTATTGTTATGTGGATCATCTGGATGGCGATGTGTTCCACATCACTGC
  TCCCAGTAAGTTCACCTTCGAGGAGGCCGAAGCAGAGTGTACAAGCAGGGATGCGAGGCTGGCGACTGTT
  GGAGAACTTCAGGCAGCTTGGAGAAATGGCTTTGACCAATGCGATTACGGCTGGCTGTCGGATGCCAGCG
  TGCGGCACCCTGTGACTGTGGCCAGGGCCCAGTGTGGAGGAGGTCTACTTGGGGTGAGAACCCTGTATCG
  TTTTGAGAACCAGACATGCTTCCCTCTCCCTGATAGCAGATTTGATGCCTACTGCTTTAAACCTAAACAG
  AATATATCAGAGGCAACCACCATCGAAATGAATATCCTCGCAGAAACTTCATCACCCAGTTTATCCAAAG
  AACCACACATGGTACCTGACAGAGCTACACCAGTCATTCCATTAGCCACCGAATTACCTATTTTTACCAC
  ACACTTCCCTCCTGCAGGAAATATAGTCAATTCTGAACAGAAATCTGTAGTCTATTCTCAGGCTATCACA
  GGCAGATTAGCCATGGAATCACCCACAACTACTCGGAATACCATCAACTCTTGGGATCTGAATGACTCCT
  TGGCTTCTGGGTCAGGGCCACTTGGAATGCCAGACATATCTGAAATTAAGGAGGAGGAACTCCGAAGTAC
  AACTGTCATCTCCCAACATGCTACTGGCTCACAGGCTGTAATTACAGAGGATACGCAAACACATGAATCA
  GTTTCACAGATTGAACAAATAGAAGTGGGCCCCTTAGTAACATCCATGGAAATCACAAATCATATTTCTC
  TCAAGGAATTGCCAGAAAAAAACAAAACACCATATGAATCCACAGAAGTGACCCTGGAACACACGACTGA
  AATGCCAACGGTAAGCGCCAGTCCTGAATTGGCAACCACAAGTCACTATGGATTCACCTTGAGAGAAGAT
  GATAGAGAAGACAGAACACTGACAGTTAGATCTGATCAGAGCACTCGGGTCTTTAGCCAAATCCCCGAAG
  TCATCACAGTATCAAAAACATCAGAAGACACTACCTACTCACAGCTAGGGGACTTAGAGTCCATTTCAAC
  ATCAACAATAACCATGCTTGGCACAGATAGATCACTGATAGATAAAGAGAAAGAACCCAAAACTAATGGT
  AAGGTGACTGAAGATGAGTTTGGTCAATCACAGCCTACAACTACCTTCCCATCACAGCATCTTACAGAAG
  TAGAATTACTTCCATATTCTGGTGACACAACATCAGTAGAGGGAATATCCACAGTTATTTATCCATCATT
  ACAAACAGATGTGACACAAGGAAGAGAGAGGACAGAAACACCAAGACCAGAGTTAAAAAAAGATCCGTAC
  ACAGTTGATGAAATACCAGAAAAGGTCACTAAAGATCCATTTATAGGTAAAACAGAAGAAGTCTTCTCTG
  GAATGCCTCTCTCTACATCTTCCTCAGAGTCCTCAGTGGAAAGGACAGAATCTGTTTCCCCTGCATTGAC
  AATTGAGAAGTTAACTGTGAAGCCAACAGAAGCAAGAGATGTGGAAGAAATGACAACCCTGACTAGATTA
  GAAACAGATGTTACAAAGTCTGACAAAGATGTTACAAGAGTTCATTTGACCCACAGTACTTTAAATGTGG
  AAGTGGTCACAGTATCAAAATGGCCAGGGGATGAAGATAATTCAACTTCAAAGCCTTTACCCTCAACAGA
  ACATGCAGGCTTTACAAAATTGCCTCCTGTCCCACTTAGTACTATAGGAATAAATGGAAAAGATAAAGAA
  ATCCCAAGTTTCACAGATGGAGGAGGTGAATACACTCTTTTCCCAGATGGTACTCCAAAACCATTAGAAA
  AGGTTTCTGAAGAAGATTTAGCATCTGGAGAATTGACAGTCACCTTTCATACATCTACATCAATTGGCAG
  TGCAGAAAAGTCAGCTTCAGGAGAGCCTACAACTGGAGACAGGTTTCTGCCCACCACAAGCACAGAAGAC
  CAGGTCATCAATGCAACTGCGGAAGGGAGTGCTTTGGGTGAAGATATAGAAGCCTCCAAGCCACTATTTA
  CTGGCCCCCCATTTGTCCACACCTCCGATGTAGAAGAATTGGCATTTGTTAATTATAGTAGCACGCAGGA
  GCCCACTACTTATGTAGACATATCCCATACTAGTCCTCTTTCTATAATTCCCAAGACAGAATGGAGTGTG
  TTGGAAACTTCTGTTTCACTAGAAGATGAGATTCTAGGCAAATCTGATCAAGATATCCTTGAACAGACTC
  ATCTAGAAGCAACCATGTCTCCTGGAGCTTTGAGGACAACAGGAGTTTCACAGGGAGAAACCCAGGAAGA
  ACCACAAACTCCAGGGAGCCCATTTCCTACTTTCAGTTCTACTGCAGTTATGGCCAAGGAGACAACAGCA
  TTTGAAGAGGGGGAGGGGTCAACATATACTCCCTCTGAAGGCAGATTGATGACAGGTTCTGAAAGAGTCC
  CAGGTTTAGAAACAACTCCAGTTGGCACATCTTATCCACCTGGTGCAATAACAGACCAGGAAGTGGAAAT
  GGATACAATGGTAACACTGATGTCAACCATTAGGCCAACAGTGGTTTCAAGTACAGAGCCAGAAGTAATT
  TATGAAGCAGAAGGTAGTAGTCCAACAGAATTTGCATCAACTTTGAGGCCTTTCCAGACTCATGTCACAC
  AGCTTATGGAAGAAACTACTGAGGAAGGAAAAAAGGCATCCTTAGATTATACAGATTTAGGCTCAGGGTT
  ATTTGAACCCAGAGCCACCGAGCTCCCAAAATTTCCAACAACTCCAAGTGATATCAGTGTCTTTACTGCC
  ATCGACAGCCTTCACAGAACGCCACCCTTGAGTCCGTCTTCATCATTCACTGAGGAACAACGTGTCTTTG
  AGGAAGAGTCCAGTGAGAAAACAACAGGTGACATACTTCCTGGAGAATCTGTAACACAGCACCCTGTGAC
  AACTCTCATTGATATCGTAGCCATGAAAACAGAATCTGATATTGATCACATGACCTCAAAACCTCCTGTA
  ACACAGCCAACAAGACCATCAGTTGTGGAAAGAAAAACAACCTCCAAAACTCAAGAGTTGTCTACTTCAA
  CACCTGCAGCAGGGACCAAGTTCCACCCTGACATAAATGTTTATATTATTGAGGTCAGAGAAAACAAGAC
  AGGACCTGATCTCTGCAAAACAAACCCATGCCTCAACGGAGGCACCTGTTATCCTACCGAGACTTCCTAT
  GTGTGCACCTGTGCACCTGGATACAGCGGAGACCAGTGTGAACTTGATTTTGATGAATGTCACTCTAACC
  CTTGTCGGAATGGTGCCACCTGTGTGGATGGTTTTAATACATTTAGATGTCTCTGTCTCCCAAGTTATGT
  TGGTGCACTCTGTGAACAAGACACTGAGACATGTGACTATGGCTGGCACAAATTCCAAGGACAGTGCTAC
  AAGTACTTTGCTCATCGACGCACATGGGATGCTGCTGAAAGGGAGTGTCGCCTGCAGGGTGCCCACCTCA
  CAAGCATCCTTTCTCATGAGGAACAAATGTTTGTGAATCGTGTGGGCCATGATTACCAGTGGATCGGCCT
  CAATGACAAGATGTTTGAACATGACTTCCGCTGGACTGACGGCAGTGCACTGCAATATGAGAACTGGAGA
  CCCAACCAGCCAGACAGTTTCTTTTCTGCAGGAGAAGACTGCGTTGTGATCATTTGGCATGAAAATGGTC
  AGTGGAATGACGTCCCCTGCAATTACCACCTCACCTACACTTGCAAGAAGGGAACAGTTGCTTGCGGCCA
  ACCCCCTGTTGTAGAAAATGCCAAGACCTTTGGAAAGATGAAACCACGCTATGAAATCAACTCCTTGATT
  AGATATCACTGCAAAGATGGTTTCATTCAGCGACACCTTCCAACTATCCGGTGCCTAGGAAACGGGAGAT
  GGGCAATGCCTAAAATAACCTGCATGAACCCATCTGCATACCAAAGGACTTATTCTAAGAAATACTTAAA
  AAATTCCTCATCCGCAAAGGACAATTCTATAAATACATCAAAACATGAGCATCGCTGGAGCCGGAGGCGG
  CAGGAAACCAGGCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001134474
• Insert Size: 4848 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134474.1, NP_001127946.1
• RefSeq Size: 7215 bp
• RefSeq ORF: 4848 bp
• Locus ID: 13003
• Cytogenetics: 13 45.5 cM