Prex2 (NM_029525) Mouse Untagged Clone

CAT#: MC224738

Prex2 (untagged) - Mouse phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (Prex2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prex2
• Synonyms: 6230420N16Rik; AI316880; AI553603; C030045D06Rik; D430013K02; Depdc2; P-Rex2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224738 representing NM_029525
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGACGAAAGCGCCAGGGAAGTAGACAAGCAGCTTCGCCTGCGCGTGTGCGTGCTCAGCGAGCTGC
  AGAAGACCGAGCGCGACTACGTGGGCACGCTGGAGTTCCTGGTGTCGGCCTTCTTGCATCGAATGAACCA
  ATGTGCAGCAGCAAAGGTTGATAAAAATGTGACGGAGGAAACAGTGAAGATGTTATTCTCCAATATCGAG
  GAGATCCTCATCGTTCATAAGGAGTTCTTGAAAGTTGTAGAAGAATGCTTATATCCGGAACCCAGTGCTC
  AGCAGGAAGTGGGAGCCTGCTTCCTTCACTTTAAGGACAAGTTTCGTATCTATGATGAATATTGCAGCAA
  CCATGAAAAGGCACAAAAATTGCTTCTTGAACTGAACAAAATAAGAACAATCCGGACATTTCTTCTGAAC
  TGCATGCTGCTTGGAGGTCGGAAGAATACAGACGTTCCCCTGGAAGGATATTTAGTGACACCAATTCAGA
  GGATATGCAAGTACCCTCTTCTTTTGAAGGAATTACTGAAGCGGACTCCACGGAGACATAGTGACTACAC
  AGCAGTGATGGAAGCACTCCAAGCCATGAAAGCCGTGTGTTCCAACATTAATGAGGCCAAGAGACAGATG
  GAGAAACTGGAAGTTTTAGAAGAGTGGCAGGCACACATTGAAGGCTGGGAGGGGTCCAACATTACTGACA
  CCTGTACTGAGATGCTGATGTGTGGAGTCCTAATGAAAATTTCTTCCGGAAATATTCAAGAACGAGTGTT
  TTTTCTTTTTGATAATCTTTTAGTATACTGCAAAAGAAAACACAGACGGCTGAAGAATAGTAAGGCATCC
  ACAGATGGGTATCGGTATGTCTTTCGAGGGCGGATCAATACTGAGGTGATGGAAGTGGAAAATGTGGATG
  ACGGCACAGCTGATTTTCACAGCAGTGGGCATATTGTTGTGAATGGGTGGAAGATACACAACACAGCAAA
  AAATAAATGGTTTGTATGCATGGCTAAAAGCCCTGAAGAAAAGCATGAGTGGTTTGAAGCTATTTTGAAA
  GAAAGAGAACGTCGGAAAGGGTTAAAACTAGGAATGGAACAAGACACCTGGGTAATGATCTCTGAGCAGG
  GTGAGAAATTGTATAAAATGATGTGCAAACAGGGAAATCTGATCAAAGACAGAAAAAGAAAACTGACCAC
  ATTTCCTAAGTGCTTCCTGGGAAGTGAATTTGTCTCATGGCTATTGGAAATTGGAGAGATTCACAGGCCC
  GAAGAAGGTGTTCACTTAGGACAAGCGCTGTTAGAGAATGGGATCATCCACCATGTTACCGATAAGCACC
  AGTTCAAACCGGAGCAAATGCTATACCGATTCCGTTATGACGATGGTACATTTTACCCCAGGAGTGAGAT
  GCAGGACGTGATTTCTAAGGGTGTAAGGCTCTACTGTCGTCTTCATAGTCTGTTTACCCCAGTGGTCAGA
  GATAAAGATTACCATTTAAGAACTTACAAATCTGTTGTCATGGCCAACAAATTGATAGACTGGTTAATTG
  CACAGGGTGATTGCCGTACCAGGGAAGAGGCAATGATATTTGCTGTTGGGCTCTGTGACAATGGATTTAT
  GCATCACGTCCTAGAAAAAAGTGAATTCAAAGATGAGCCTCTACTTTTCCGCTTTTTTGCTGATGAGGAA
  ATGGAAGGATCAAATATGAAGCATAGGCTTATGAAACATGACTTGAAGGTTGTGGAGAATGTCATAGCTA
  AGTCACTGTTGATTAAATCCAACGAAGGCAGCTATGGCTTTGGCCTAGAAGACAAAAACAAAGTTCCAAT
  AATAAAATTAGTGGAAAAGGGGTCGAATGCCGAGATGGCTGGCATGGAAGTTGGGAAGAAGATATTTGCC
  ATTAATGGTGACCTTGTTTTTCTGAGACCTTTTCCTGAAGTCGATTGCTTCCTGAAATCGTGCTTAAACA
  GCAGAAAGCCACTGAGAGTCCTCGTGAGCACAAAGCCAAGAGAGACCGTTAAGATTCCAGATTCTGCAGA
  CGGGCTTGGTTTCCAGATCAGAGGCTTTGGACCTTCAGTTGTGCATGCGGTGGGAAGAGGTACTGTGGCT
  GCAGCAGCTGGCCTTCATCCAGGACAGTGTATTATCAAAGTGAATGGAATCAATGTCAGCAAAGAGACAC
  ATGCCAGTGTCATCGCACATGTCACAGCCTGCCGGAAATACAAACGGCCAATGAAGCAAGATTCCATACA
  GTGGGTCTACGACAGTCTTGAAAGTGCTCAGGAAGACATTCAGAAGTCCCACTCAAAGCCCCCTGGAGAC
  GGGGCTGGAGATGCTTTTGAGTGCAAAGTAGAAGATGTCATTGACAAGTTCAACACCATGGCTATTATTG
  ATGGCAAGAAGGAGCATGTGAGTCTGACGGTGGACAACGTCCACCTCGAGTATGGGGTTGTATATGAGTA
  TGATAGCACGGCGGGCACAAAGTGCAATGTGGTGGAGAAGATGGTGGAGCCCAAAGGGTTCTTCAGCCTA
  ACTGCCAAGATTCTGGAAGCCCTAGCAAAAAGTGACGAACATTTTGTCCAAAACTGCACCAGCCTTAATT
  CTCTGAATGAAGTGATCGCCACTGATCTTCAGAGTAAATTCACCAGCATGTGCAGTGAGAGAATCGAACA
  TGTGTGTCACAGAATATCCAGCTATGGGAGGTTCTCTCGGGTGCTGAAGAACAGAGCCTGGCCCACCTTT
  AAGCAGGCCAAACCTAAGATCTCCCCACTGCACAGCAGTGATTTCTGCCCAACCAACTGCCACGTCAATG
  TGATGGAAGTTTCTTATCCGAAAACATCAACTTCCCTGGGGAGTGCCTTCGGTGTGCAGCTAGATAGCAG
  AAAACATAATTCTCACGATAAAGAAAATAAATCTGTCGAGCCAGGGAAACTGAGCCCCATGGTGTATATT
  CAGCACACAATCACAACCATGGCAGCGCCTTCGGGCCTGTCACTGGGACACAAGGATGGGCATGGCCTCC
  AGTATTTGCTGAAAGAAGAAGACTTAGAAACTCAAGATATCTATCACAAACTTCTGGGCAAGCTGCAGAC
  TGCACTGAAGGAGGTAGAGATGAGCGTTTGTCAAATTGATGACCTTCTGTCTTCAATAACATATTCTCCT
  AAATTGGAACGTAAGACAACAGAGTGTGTGACACCAATGGACAGTGACAACGAGAAGGGAGAGAGAAATA
  GCAAACGTGTCTGTTTTAATGTAGCAGGGGATGAGCAAGAGGATTCAGGCCATGATACAGTCAGCAACAG
  AGACTCTTACAGTGACTGCAACAGCAACAGGAATTCCATTGCTTCCTTCACGAGTATCTGCAGCAGCCAG
  TGCAGTTCCTACTTCCACAGTGATGAGATGGACTCAGGTGATGAGCTTCCTATCAGTGTCCGGATATCTC
  ATGATAAACAAGACAAGATCCACACTTGCCTGGAACAGCTCTTCAGCCAGATTGATTCAATAATCAACCT
  CTTGAAAGGACAAGCTGTTATAAGAGCCTTTGAGCAGACCAAGTATCTCACACCGGGTCGAGGATTGCAA
  GAATTCCAACAGGAAATGGAGGCAAAGCTGAGTTGCCCCAGGAGGTTGCGGCTCCATCTCAAGCAGGACC
  CCTGGAATCTTCCCAGCAGCATCCAGGCGCTTGCACAGAGCATCAGAAAACATGCTGAAGAAGTGAAGTG
  CCGCATACTACTGGCTCTCCTTGAATACTCAGACAGTGAAACCCAACTCCGGAGAGACATGGTTTTCTGT
  CAAAGTCTTGTGGCCACTGTCTGTGCCTTCTCTGAGCAGCTCATGGCTGCCTTAAACCAGATGTTTGACA
  ACAGCAAGGAAAATGAAATGGAGACTTGTGAAGCCAGCAGGAGGTGGCTGGACCAGATTGCAAATGCTGG
  TGTGCTTTTTCACTTCCAGTCACTTCTGTCACCAAATTTGAAAGATGAACAAGCCATGCTTGAGGATACA
  CTGGTTGCCCTGTTTGATTTGGAAAAAGTTTCCTTCTTCTTCAAACCATCAGAAGAGGATCCACTGGTTG
  CAAATGTCCCTCTTACCTACCAAGTGGAAGGAAGCAGGCAGGCTCTGAAAGTTTACTTCTACATGGATAG
  TTACCATTTTGAGCAGCTGCCCCAACGGTTGAAGAATGGAGGAGGGTTTAAAATTCATCCTGTTCTCTTT
  TCACAAGCATTGGAGAGTATGGAAGGATATTGCTATAGAGACAACATCTCTGTGGAAGAGTTTCAAGCAC
  AAATAAACACAGCCTCCCTGGAAAAGGTCAAACAGTACAATCAGAAGCTCAGGGCATTCTACTTGGACAA
  GTCAAATTCCCCACCGAATACCACATCTAAAGCTGCCTACATAGATAAGCTCATGAAGCCTCTCAATGCT
  TTAGATGAACTTTACCGGCTGATCACCTCATTTATCAGATCCAAACGCATCGCTGCCTGTGTGAACACAC
  CTTGCAGCGCCTCTGGAGTCGGACTGCTGTCAGTTTCTTCAGAGCTGTGTGACAGGCTCGGTGCCTGTCA
  CATCATCATGTGCAGCAGTGGTGTGCACCGGTGCACCCTGAGTGTGACACTAGAGCAAACCATCACTTTA
  GCAAGAAGCCATGGGCTCCCTCCTCGGTACATTATGCAGGCCATGGATGTGATGAGAAAGCAGGGTGCAA
  GAGTTCAGAACACAGCTAAAAACTTGGGCGTTAGAGACCGGACTCCTCAGTCAGCTCCAAGGCTGTACAA
  GCTCTGCGAGCCACCACCTCCAGTAGGAGAAGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029525
• Insert Size: 4797 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029525.1, NP_083801.1
• RefSeq Size: 11057 bp
• RefSeq ORF: 4797 bp
• Locus ID: 109294
• UniProt ID: Q3LAC4
• Cytogenetics: 1 A2
• Gene Summary: Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer variant and it encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.