Dlc1 (NM_001194940) Mouse Untagged Clone

CAT#: MC224663

Dlc1 (untagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dlc1
• Synonyms: A730069N07Rik; Arhgap7; dlc-1; HP; STARD12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224663 representing NM_001194940
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTAGCTATCAGAAAGAGGAGCTGGGAAGAGCACGTGACCCAGCAGACCGAACAGCCTTTTATTT
  CTGATGAGTATGACATAGCATGTCACCACGGACTGGCATCCGACAGCTTGGAGGGAAGGATGGAAAAAGA
  TGCGACCTTAAATGTGGACCATAAAGAGAAGTGTGCCTCTCTACCGGACCGCTGTTGTGGGTCAGAGACA
  AGAGACTTTCCTGGGAGGCCAATGGGTCACATTGCACAGGAGGTAGATGAAAGTGACAGCCAAGAAGGGG
  AAGAGCGGTTTCTATCGCTCGAGGCCAGCACAGAAACACTAGTGCACATTTCGGATGAGGATACTGATTC
  TGATCTAAACCTTATCAATGACTCACAGATTTTAACTCCGCAAAGACATGAAAGAGACAGCCAAGATAGT
  GTCAAAGGAGCAGGCTCCTTCATGAAGACACTGTCCACCATGCAAAGTAGCCAAGATTCTCATAACACCT
  GGAGGATGGCTGGTAAGGCTGATATGTCTCCAGCAGAAGGAAGTGGAGAAAGGAAAGATGTTGAGGCTAT
  AAATAAAAGCCTGGGGCTTTGTAAGAATACGAGTGCAGATGAAACAAAAGATGTGCCCATAGGAAATACA
  TTCCAGTCTTTGAATACGGAAGATGACGTCACTCCTAAGACACTACTACTTGATTCTTCGGTACTCATAG
  AAGATGTCACACCTGAGACACAACTGCTTGATTCTGCAGTCATCACTCAACAATGCAGGAAATCTGACTT
  CCCTAAAGATGACCAGGAAAATAATCGGCACCATGTCTTAGAAGAAGACGTCTTAGCAGCTCCTTATGTA
  GACAGAGGGCTGCCATTATTAAAAGCAGATTGCAGAAGCAGCCTTCTTCAGCCTCCTTCCTGCCCTGGTG
  GGATGTCAGCAGAAAATGACCTGGAGAAGAGTGGATTCTCAGAATATCAAAACAAAAGCACTCTCGGGGT
  CAGCAGGGAAGATGGCATGCAGTGTTTACACTTAAGGGGCCCCGTGACCACCCAGGAGACTGTAGACAAT
  CAAGTCAGACTGCGCAAGAGAAAGGAAACAAGGGAAGATCGAGACAGGACACGGCTGGACTCCATGGTGC
  TGCTCATTATGAAACTGGACCAACTAGACCAAGACATAGAGAATGCTCTCAGCACTGCCTCCTCTCCATC
  CAGCACCCCAACGAACCTGCGGCGGCATGTCCCTGATCTGGAATCAGGATCTGAAAGCAGAACAGACAGT
  ATGGCAGTAAATCAGACACAGGTGAATCTGTCTTCAGAGTCCACCGATCCATCATCCACTCCAGGGAGCA
  ATTCTGGAACCAAACCCAAAGCCATGGCTGCTCCGTGTGTTGGAGAAAAGGAAATGGCTGAAATTGAAGC
  CAAGGAAGCTTGCGACTGGCTGAGAGTGACTGGTTTCCCCCAGTATGCACAGCTGTATGAAGATCTACTG
  TTCCCTATTGATATTGCGCTGGTCAAGAGAGAACATGACTTCTTGGACAGAGACGCCATCGAGGCTCTCT
  GCAGGCGCTTAAATACTCTAAACAAGTGTGCAGTCATGAAGCTGGAGATCAGTCCTCACCGGAAGCGAAG
  TGAGGATTCGGATGAAGATGAGCCTTGCGCCATAAGCGGCAAGTGGACTTTCCAGAGGGACAGCAAGCGG
  TGGTCCCGCCTTGAAGAGTTTGACGTCTTTTCCCCAAAGCAGGATCCAATCCCTGGGTCCCCAGACAACT
  CTCGTTTGCAAAGCGCCACAAGCCACGAAAGCATGCTGACAGACCTCAGCGAGCACCAGGAGGTGGCCTC
  TGTCCGAAGCCTCAGCAGCACCAGCAGCAGCGTCCCCACCCACGCAGCCCACAGTGGAGATGCCACTACG
  CCCCGAACCAATTCCGTCATCAGCGTCTGCTCCTCCGGACACTTTGTAGGCAATGATGACTCTTTTTCCA
  GCCTGCCGTCTCCCAAGGAACTGTCCAGCTTCAGTTTTAGCATGAAAGGCCACCACGAGAAGAACACCAA
  GTCGAAGACGCGGAGCCTGCTCAAACGCATGGAGAGCCTGAAGCTCAAGGGCTCCCACCACAGCAAGCAC
  AAGGCGCCTTCCAAGCTGGGGTTGATCATCAGTGCTCCCATTCTGCAGGAGGGTATGGATGAGGCAAAGC
  TGAAGCAGCTGAACTGTGTGGAGATCTCAGCCCTCAATGGCAACCACATCAACGTGCCCATGGTACGGAA
  AAGGAGCGTGTCTAACTCCACCCAGACCAGCAGCAGCAGCAGCCAATCAGAGACCAGCAGCGCGGTCAGC
  ACACCCAGCCCGGTCACCAGGACCCGGAGCCTCAGCACCTGTAACAAGCGGGTGGGCATGTATCTAGAGG
  GCTTCGACCCATTCAGTCAGTCCACCTTGAACAACGTGACGGAGCAGAACTATAAAAACCGTGAGAGCTA
  CCCAGAGGACACGGTGTTCTACATTCCCGAAGATCACAAGCCCGGCACCTTCCCTAAGGCCCTCTCCCAT
  GGCAGTTTCTGTCCCTCGGGAAACAGTTCTGTGAACTGGAGGACCGGAAGCTTCCATGGCCCTGGCCATC
  TCAGCCTACGGAGAGAAAACAGCCATGACAGTCCTAAGGAGCTGAAGAGACGCAATTCTTCCAGCTCTCT
  GAGCAGCCGCCTGAGCATCTATGATAACGTACCGGGTTCTATCCTGTACTCCAGCTCGGGAGAACTGGCC
  GACCTGGAGAATGAGGACATCTTCCCTGAGCTGGATGACATTCTCTACCACGTGAAGGGGATGCAGCGGA
  TAGTCAACCAGTGGTCCGAGAAGTTTTCCGACGAGGGAGACTCGGACTCAGCCCTGGACTCTGTCTCTCC
  TTGCCCGTCATCTCCAAAACAGATACACCTGGATGTGGACCATGACCGAAGGACACCCAGTGACCTGGAC
  AGCACAGGCAACTCCCTCAATGAGCCCGAAGAGCCCACTGATATCCCGGAAAGAAGAGACTCAGGGGTGG
  GGGCTTCCCTGACCAGGTGCAATAGGCACAGACTGAGGTGGCACAGCTTCCAGAGCTCCCACCGGCCGAG
  CCTCAACTCCGTGTCACTGCAGATTAACTGCCAGTCTGTGGCCCAGATGAACCTGCTGCAGAAATACTCG
  CTCCTGAAATTGACGGCCCTGCTGGAAAAGTACACGCCTTCCAACAAGCACGGCTTCAGCTGGGCTGTGC
  CCAAGTTCATGAAAAGGATCAAGGTTCCAGACTACAAGGACCGGAGTGTGTTTGGGGTCCCACTGACTGT
  GAACGTGCAGCGCTCAGGACAGCCCCTGCCCCAGAGCATCCAGCAGGCCATGCGCTACCTCCGTAACCAC
  TGTCTGGACCAGGTCGGGCTCTTCAGGAAGTCAGGTGTCAAATCCCGGATCCAGGCTCTACGCCAGATGA
  ATGAAAGCGCTGAAGATAATGTCAACTATGAAGGCCAGTCTGCTTATGATGTGGCAGACATGTTAAAGCA
  ATATTTTCGAGATCTTCCTGAGCCCCTCATGACGAACAAACTCTCCGAAACCTTCCTGCAGATCTACCAG
  TATGTTCCCAAGGACCAGCGCCTCCAAGCCATCAAGGCGGCCATTATGCTCCTGCCCGACGAGAACCGGG
  AGGTTCTGCAGACACTTCTCTATTTCCTGAGCGATGTCACAGCGGCTGTGAAAGAAAACCAGATGACTCC
  CACCAACCTGGCTGTGTGCCTAGCTCCGTCCCTCTTCCACCTCAACACCCTGAAGCGAGAGAATTCTTCT
  CCAAGGGTAATGCAAAGAAAACAGAGTTTGGGCAAACCAGACCAGAAAGACCTGAATGAAAACCTAGCGG
  CGACTCAAGGGCTGGCCCACATGATTGCTGAGTGCAAGAAGCTCTTCCAGGTCCCTGAGGAAATGAGCCG
  GTGCCGTAACTCCTACACTGAACAAGAGCTGAAGCCCCTTACCCTGGAGGCCTTGGGACACCTGAATAGT
  GACCAGCCTGCTGACTACAGACACTTCCTCCAGGACTGTGTGGATGGCCTGTTTAAGGAGGTCAAAGAGA
  AGTTCAAAGGCTGGGTCAGCTACCCCACCTCCGAACAGGCTGAGCTGTCCTATAAGAAGGTCAGCGAAGG
  ACCCCCGTTAAGGCTTTGGAGGTCAACTATCGAAGTCCCCGCTGCACCCGAGGAGATCTTAAAGCGCCTT
  CTGAAGGAGCAACACCTCTGGGATGTGGACCTGCTGGACTCCAAGGTGATTGAAATCCTGGACAGCCAGA
  CTGAAATCTACCAATACGTCCAAAACAGCATGGCGCCCCACCCTGCGCGGGACTACGTCGTATTGAGGAC
  CTGGAGGACTAACTTACCCAGGGGGGCCTGTGCTCTCTTACTCACCTCTGTGGATCACGACCGGGCCCCC
  GTGGCGGGGGTGAGGGTTAATGTGCTCCTGTCCAGATATTTGATTGAACCCTGCGGGTCAGGAAAATCCA
  AGCTTACCTACATGTGCAGAGCTGATTTAAGGGGCCACATGCCGGAGTGGTACTCAAAATCTTTTGGACA
  TTTGTGTGCAGCCGAAGTGGTAAAGATCCGAGACTCCTTCAGTAATCAGAACACTGAAAGCAAGGACACG
  AGATCTAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001194940
• Insert Size: 4632 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001194940.2, NP_001181869.1
• RefSeq Size: 7636 bp
• RefSeq ORF: 4632 bp
• Locus ID: 50768
• Cytogenetics: 8 23.05 cM
• Gene Summary: Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.