Robo2 (NM_175549) Mouse Untagged Clone

CAT#: MC224626

Robo2 (untagged) - Mouse roundabout homolog 2 (Drosophila) (Robo2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Robo2
• Synonyms: 2600013A04Rik; 9430089E08Rik; BB097918; D230004I22Rik; mKIAA1568
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224626 representing NM_175549
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCTCTGATGTTTACACTATTATTGCTCTTTGGATTTCTCTGCATTCAGATTGATGGATCTCGTC
  TTCGTCAAGAAGACTTTCCCCCCAGGATCGTAGAACACCCTTCTGATGTCATCGTCTCCAAGGGAGAGCC
  CACCACTCTGAACTGTAAAGCAGAGGGCCGACCCACCCCCACCATTGAATGGTACAAGGATGGTGAGAGG
  GTGGAGACAGACAAGGATGATCCCAGGTCCCACAGAATGCTTCTGCCCAGCGGATCTTTATTCTTTTTGC
  GAATTGTTCATGGGCGCAGAAGTAAACCGGACGAAGGGAGTTACGTTTGTGTTGCAAGGAACTATCTTGG
  TGAAGCAGTGAGTCGAAATGCATCTCTGGAAGTGGCATTATTGCGAGATGACTTCCGGCAAAACCCCACA
  GATGTGGTAGTCGCAGCTGGAGAGCCTGCAATCTTGGAGTGCCAGCCACCACGGGGACACCCAGAACCAA
  CCATCTACTGGAAAAAGGACAAAGTCCGAATTGATGACAAGGAAGAGAGAATAAGTATCCGTGGTGGGAA
  GCTGATGATCTCTAATACTAGGAAAAGCGATGCTGGCATGTACACCTGTGTGGGAACCAATATGGTGGGA
  GAAAGGGACAGCGACCCTGCAGAGCTCACTGTCTTTGAACGACCCACATTTCTCAGGAGGCCAATTAACC
  AGGTGGTGCTAGAGGAAGAAGCTGTAGAATTCCGTTGTCAGGTCCAAGGAGATCCCCAGCCAACGGTGAG
  GTGGAAAAAAGATGATGCAGACTTGCCGAGAGGAAGGTATGATATCAAAGATGACTACACGCTGAGAATT
  AAAAAGGCCATGAGTACTGATGAAGGTACCTATGTGTGTATTGCTGAGAATCGGGTGGGAAAAGTGGAAG
  CCTCTGCTACCCTCACTGTCCGAGCTCCTCCACAGTTTGTGGTTAGGCCAAGAGATCAGATCGTTGCTCA
  AGGCCGAACAGTGACATTCCCCTGTGAAACTAAAGGAAACCCACAGCCAGCTGTTTTTTGGCAGAAAGAA
  GGCAGCCAGAACCTACTTTTCCCGAATCAACCTCAGCAGCCCAACAGCCGATGTTCAGTGTCGCCCACGG
  GGGACCTCACCATCACCAACATCCAGCGTTCAGATGCGGGTTACTACATCTGCCAGGCCCTAACCGTGGC
  AGGAAGCATTTTAGCTAAAGCACAGTTGGAAGTTACTGACGTTTTGACAGATAGACCTCCACCCATAATC
  TTGCAAGGACCAATAAACCAAACACTTGCAGTAGACGGTACAGCATTGTTGAAGTGTAAAGCCACTGGTG
  AGCCTCTGCCTGTAATTAGCTGGCTAAAGGAGGGCTTTACTTTTCTGGGGAGAGATCCAAGAGCCACGAT
  CCAAGACCAAGGAACACTGCAGATTAAGAATTTACGGATATCTGATACTGGCACTTATACTTGTGTGGCT
  ACAAGTTCCAGTGGAGAGACTTCCTGGAGTGCAGTGCTGGATGTAACAGAATCTGGAGCAACAATCAGTA
  AAAATTATGATATGAATGACCTCCCGGGACCACCATCCAAACCTCAGGTCACTGATGTTTCTAAGAACAG
  TGTCACCTTATCCTGGCAGCCAGGTACACCTGGCGTTCTTCCTGCAAGCGCGTATATCATTGAGGCTTTC
  AGCCAATCGGTGAGCAATAGCTGGCAGACAGTGGCAAACCATGTTAAGACAACTCTGTATACAGTAAGGG
  GGCTGAGGCCCAACACAATCTACTTGTTTATGGTCAGAGCGATCAACCCACAAGGTCTCAGTGATCCAAG
  TCCTATGTCGGATCCTGTACGCACACAAGATATCAGCCCCCCAGCACAAGGAGTGGACCACAGACAGGTG
  CAGAAGGAATTAGGTGATGTCGTTGTTCGTCTCCATAATCCAGTTGTCCTGACACCTACAACTGTTCAAG
  TCACATGGACGGTGGACCGACAACCCCAGTTTATTCAGGGCTACAGAGTGATGTACCGTCAGACTTCGGG
  ACTACAAGCCTCAACTGTGTGGCAGAATCTAGACGCCAAAGTCCCGACTGAGAGGAGTGCTGTCCTTGTG
  AATTTGAAAAAGGGGGTGACTTATGAAATTAAAGTCCGGCCGTATTTTAACGAGTTCCAAGGAATGGACA
  GTGAATCGAAAACAGTCCGAACCACTGAGGAAGCCCCAAGTGCCCCTCCCCAGTCTGTCACTGTGCTGAC
  AGTTGGAAGTCACAACAGCACAAGCATCAGTGTTTCCTGGGATCCTCCACCAGCCGACCACCAGAATGGA
  ATTATTCAGGAATATAAGATCTGGTGTCTGGGAAACGAAACGCGATTCCATATCAATAAAACGGTGGATG
  CAGCCATTCGCTCTGTAGTAATAGGTGGCTTGTTCCCTGGAATTCAGTACCGGGTAGAAGTGGCAGCTAG
  CACAAGTGCAGGGGTTGGAGTAAAAAGTGAACCACAGCCGATAATAATTGGGGGACGTAACGAAGTTGTC
  ATTACTGAAAACAATAACAGCATCACTGAGCAAATCACGGATGTCGTGAAGCAACCGGCATTTATAGCTG
  GCATTGGTGGTGCCTGCTGGGTAATTCTGATGGGTTTTAGCATCTGGTTGTACTGGAGAAGAAAGAAGAG
  AAAGGGACTCAGCAATTATGCTGTAACATTTCAAAGAGGAGATGGAGGACTAATGAGCAATGGGAGCCGT
  CCAGGTCTTCTAAATGCTGGCGATCCCAATTACCCATGGCTTGCTGATTCTTGGCCAGCCACGAGTTTGC
  CAGTGAACAATAGCAATAGTGGCCCAAATGAAATTGGAAATTTTGGGCGTGGAGATGTGCTGCCTCCGGT
  GCCAGGCCAAGGGGATAAAACAGCGACCATGCTCTCGGATGGAGCCATTTATAGCAGCATTGACTTCACT
  ACCAAAACCACTTACAACAGTTCCAGCCAAATAACACAGGCCACCCCATATGCCACTACACAAATCCTGC
  ATTCAAACAGCATCCACGAACTGGCAGTTGATCTTCCTGATCCACAGTGGAAAAGCTCAGTTCAACAGAA
  GACAGACCTCATGGGATTTGGTTATTCGCTACCTGATCAGAACAAGGGGAACAACGCCTTACTTTACATC
  CCTGACTACCGATTGGCTGAGGGATTGTCTAATAGAATGCCACACAACCAGTCACAGGATTTCAGCACCA
  CCAGCTCTCACAACAGCTCAGAAAGGAGTGGCAGTCTCTCAGGTGGGAAAGGTGGAAAAAAGAAGAAAAC
  TAAAAATTCTTCGAAAGCGCAGAAAAACAACGGATCCACTTGGGCTAATGTCCCTCTACCTCCTCCTCCC
  GTCCAGCCCCTTCCTGGTACAGAGCTGGGCCACTATGCTGCGGAACAAGAAAATGGCTATGACAGTGACA
  GCTGGTGCCCACCATTACCGGTGCAAACATACTTGCATCAGGGTATGGAAGATGAGCTGGAAGAAGACGA
  AGATCGGGTCCCAACACCTCCTGTTCGCGGCGTGGCCTCTTCACCAGCTATCTCTTTTGGACAGCAGTCC
  ACTGCCACTCTTACTCCATCCCCACGGGAAGAGATGCAACCCATGCTGCAAGCTCACTTGGATGAGTTGA
  CAAGGGCCTATCAGTTTGATATAGCAAAACAAACATGGCACATTCAAAGCAATACCCCACCTCCACAACC
  CCCAGCTCCGCCATTAGGTTACGTGTCCGGAGCCTTGATTTCTGATTTGGAGACAGATGTTCCAGATGAG
  GATGCTGATGATGAAGAGGAACCATTAGAAATTCCCAGGCCCCTCAGAGCACTAGACCAGACACCTGGAT
  CCAGTATGGACAATCTAGACAGCTCTGTCACAGGAAAAGCCTTTAGCTCCTCTCAAAGGCAGCGGCCCAC
  CAGCCCATTTTCTACGGACAGTAACACCAGTGCTGCCCAGAATCAAAGCCAGAGGCCTCGGCCCACGAAA
  AAACATAAGGGAGGACGGATGGACCCACAGCCAGTGTTACCTCATCGGAGGGAAGGGATGCCAGATGATC
  TTCCCCCACCACCAGATCCCCCACCAGGTCAGGGTTTAAGGCAGCAGATAGGCCTGAGCCAACACTCTGG
  TAACGTGGAAAACTCAACAGAGAGAAAAGGAAGCTCTCTAGAGAGACAACAAGCAGCCAACTTAGAAGAC
  ACAAAGAGCTCATTGGATTGTCCAGCTAAAACTGTTCTAGAGTGGCAGCGTCAAACCCAGGACTGGATAA
  ACTCTACAGAACGCCAAGAAGAAACTCGGAAAGCCCCACACAAACAAGGGGTTGGATCAGAGGAGTCCCT
  GGTGCCCTACAGCAAGCCCAGCTTCCCGTCTCCTGGCGGGCACAGCTCATCAGGAACATCTTCCTCTAAA
  GGGTCCACTGGCCCTCGGAAGGCTGACGTGCTGAGAGGCAGCCACCAGAGGAATGCCAATGACCTTCTCG
  ACATTGGATATGTGGGCTCAAATAGTCAAGGACAGTTTACAGAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175549
• Insert Size: 4527 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175549.4, NP_780758.3
• RefSeq Size: 8047 bp
• RefSeq ORF: 4527 bp
• Locus ID: 268902
• Cytogenetics: 16 C3.1