Atp7a (NM_009726) Mouse Untagged Clone

CAT#: MC224551

Atp7a (untagged) - Mouse ATPase, Cu++ transporting, alpha polypeptide (Atp7a), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp7a
• Synonyms: MNK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224551 representing NM_009726
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCAAGTGTGGATGCAAATTCAATTACTATCACTGTTGAGGGAATGACATGTATTTCCTGTGTCC
  GGACCATTGAACAGCAGATTGGGAAAGTGAATGGTGTCCATCACATTAAAGTTTCACTAGAAGAAAAGAG
  TGCAACCATTATTTATGACCCTAAACTTCAGACTCCAAAGACCCTCCAAGAAGCTATCGATGACATGGGC
  TTTGATGCTCTTCTCCACAATGCTAACCCCCTCCCTGTCTTAACCAATACCGTGTTCCTGACTGTTACTG
  CTCCACTGACTCTGCCTTGGGACCATATCCAAAGTACATTGCTCAAGACCAAGGGTGTGACTGGTGTTAA
  GATTTCCCCTCAGCAAAGAAGTGCAGTAGTCACCATAATTCCATCTGTGGTGAGTGCCAGTCAGATTGTG
  GAGCTGGTCCCAGACCTCAGTTTAGACATGGGAACTCAGGAGAAAAAGTCAGGAGCTTGTGAAGAGCACA
  GCACGCCTCAGGCAGGGGAAGTCATGCTGAAGATGAAAGTGGAAGGGATGACCTGCCATTCATGTACTAG
  CACCATTGAAGGAAAAGTTGGAAAGCTGCAAGGTGTTCAGCGCATTAAAGTGTCCCTAGACAACCAAGAA
  GCTACTATTGTTTTTCAACCTCATCTCATCACAGCAGAGGAAATAAAGAAGCAGATTGAAGCCGTGGGTT
  TCCCAGCCTTCATAAAAAAGCAGCCGAAGTACCTCAAATTGGGAGCCATTGATGTTGAGCGCCTGAAGAA
  CACACCGGTCAAATCTTCAGAAGGATCTCAGCAAAAGAGCCCATCATATCCCAGTGACTCCACAACCATG
  TTCACCATAGAGGGCATGCATTGTAAATCGTGTGTGTCCAATATTGAAAGTGCTTTATCTACACTCCAGT
  ATGTAAGCAGTATAGTAGTTTCTTTAGAGAATAGGTCAGCCATTGTAAAGTACAATGCAAGCTTAGTCAC
  TCCAGAAATGCTGAGAAAGGCAATAGAGGCCATTTCACCGGGGCAATACAGAGTTAGTATTGCAAGTGAA
  GTTGAAAGTACCGCCAGCTCTCCCTCCAGCTCATCTCTTCAGAAGATGCCTTTGAACATAGTTAGCCAGC
  CTCTGACCCAAGAAGCTGTGATAAACATAAATGGCATGACTTGTAATTCTTGTGTGCAGTCTATAGAGGG
  TGTAATATCAAAAAAGCCAGGTGTAAAATCCATCCACGTGTCCCTCGCAAACAGCACAGGGACTATTGAA
  TTTGATCCTCTACTAACCTCTCCAGAAACCTTGCGAGAAGCAATTGAAGACATGGGATTTGATGCTGCCT
  TGCCAGACATGAAAGAGCCACTGGTAGTGATAGCTCAGCCCTCACTGGAAACACCTCTTTTGCCCTCAAG
  TAATGAGCTAGAAAATGTGATGACGTCAGTTCAGAACAAGTGTTACATACAGGTCTCTGGGATGACCTGT
  GCTTCTTGTGTAGCAAACATTGAACGCAATTTAAGACGAGAAGAAGGAATATATTCTGTACTTGTTGCCC
  TAATGGCTGGCAAGGCAGAAGTAAGATATAACCCAGCTGTTATCCAACCCCGAGTGATAGCAGAGTTTAT
  TCGAGAGCTTGGATTTGGAGCTATGGTGATGGAAAACGCTGGGGAAGGCAACGGCATCTTGGAACTTGTT
  GTGAGAGGAATGACGTGTGCTTCCTGTGTCCATAAAATTGAATCAACACTCACAAAACACAAAGGGATCT
  TCTACTGCTCTGTGGCCCTGGCAACTAACAAAGCACATATTAAATATGATCCAGAAATTATTGGTCCCAG
  AGATATTATCCATACCATCGGAAGCTTAGGCTTTGAAGCTTCTTTGGTCAAGAAAGATCGATCAGCCAAC
  CACTTAGATCATAAACGAGAAATAAAACAATGGAGAGGGTCTTTCCTTGTGAGCCTGTTTTTCTGTATCC
  CTGTAATGGGACTGATGGTCTATATGATGGTTATGGATCACCACCTTGCAACTCTTCACCATAATCAGAA
  CATGAGTAATGAAGAAATGATCAACATGCATTCTGCTATGTTTCTGGAGCGTCAGATCCTGCCAGGACTG
  TCCATTATGAATTTGTTGTCCCTTTTATTGTGTCTACCTGTACAGTTTTGTGGCGGCTGGTACTTCTACA
  TTCAGGCTTACAAAGCACTGAAGCATAAGACAGCAAACATGGATGTGCTGATTGTGCTGGCAACCACCAT
  CGCATTTGCCTACTCTCTGGTTATTCTTCTGGTTGCAATGTTTGAGAGAGCCAAAGTGAACCCCATTACC
  TTCTTTGATACACCTCCTATGCTGTTTGTGTTTATTGCACTAGGACGATGGCTGGAACATATAGCGAAGG
  GCAAAACCTCCGAGGCTCTTGCAAAGCTAATTTCATTACAAGCAACTGAAGCCACTATTGTAACTCTGAA
  CTCTGAAAATCTCCTCCTGAGTGAAGAACAAGTGGATGTGGAACTTGTACAACGTGGAGATATCATTAAG
  GTTGTTCCAGGAGGCAAATTTCCAGTGGATGGCCGTGTTATTGAAGGACATTCTATGGTGGACGAGTCCC
  TCATCACAGGGGAGGCAATGCCTGTGGCTAAGAAACCTGGCAGCACGGTGATTGCAGGTTCCATTAACCA
  GAATGGATCTCTCCTCATCCGAGCAACACATGTGGGAGCAGATACAACCCTTTCTCAGATCGTCAAACTT
  GTGGAGGAGGCACAGACATCAAAGGCTCCTATCCAGCAGTTTGCAGACAAACTCAGTGGCTACTTTGTTC
  CTTTCATCGTCTTGGTTTCCATTGTTACCCTCTTGGTGTGGATTATAATTGGATTTCAAAATTTTGAAAT
  TGTGGAAACCTACTTTCCCGGCTATAATAGAAGCATCTCCCGAACAGAAACCATAATACGCTTTGCTTTC
  CAAGCCTCTATCACGGTTCTGTGTATCGCATGTCCCTGTTCACTGGGACTAGCCACTCCAACTGCTGTGA
  TGGTGGGCACAGGAGTAGGTGCTCAGAATGGCATACTTATCAAAGGTGGGGAGCCACTGGAAATGGCTCA
  TAAGGTAAAGGTAGTGGTATTTGATAAGACTGGAACCATCACCCATGGAACCCCAGTAGTGAATCAAGTG
  AAGGTTCTCGTGGAAAGTAACAAGATATCACGCAATAAGATCCTGGCCATTGTGGGGACTGCAGAAAGTA
  ACAGTGAACATCCTTTAGGAGCAGCTGTAACCAAATATTGCAAAAAGGAGCTGGACACTGAAACCCTGGG
  TACCTGTACAGATTTCCAGGTTGTACCAGGCTGTGGTATTAGCTGTAAAGTCACCAATATTGAAGGTTTG
  CTACATAAGAGTAACTTGAAGATAGAAGAAAATAACATTAAAAATGCATCCCTGGTTCAAATTGATGCAA
  TTAATGAACAGTCATCAACTTCATCTTCTATGATTATTGATGCTCATCTCTCAAATGCTGTTAATACTCA
  GCAGTACAAAGTCCTCATTGGTAACCGGGAATGGATGATTAGAAATGGTCTTGTCATAAGTAATGATGTA
  GATGAATCTATGATTGAACATGAAAGAAGAGGTCGGACTGCTGTCTTGGTGACAATCGATGATGAGCTGT
  GTGGCTTGATTGCTATTGCTGATACTGTGAAACCTGAGGCCGAGTTGGCTGTACACATTCTGAAATCTAT
  GGGTTTAGAAGTAGTTCTGATGACTGGAGACAACAGTAAAACTGCTCGGTCTATTGCTTCTCAGGTTGGC
  ATTACTAAGGTGTTTGCTGAAGTTCTACCTTCCCACAAAGTTGCTAAGGTGAAGCAGCTCCAAGAGGAGG
  GCAAACGTGTAGCAATGGTAGGAGATGGAATCAATGACTCCCCAGCTCTGGCAATGGCAAACGTTGGAAT
  TGCCATAGGCACAGGCACAGATGTAGCCATTGAAGCAGCTGATGTGGTTTTGATAAGGAATGACCTTCTG
  GATGTTGTGGCAAGTATTGACTTGTCAAGGAAAACAGTCAAGAGGATTCGAATCAATTTTGTCTTTGCTC
  TAATTTATAATCTGGTTGGAATTCCCATCGCTGCTGGAGTTTTCCTGCCCATCGGTTTGGTTTTACAACC
  CTGGATGGGATCCGCAGCAATGGCCGCTTCATCTGTCTCTGTAGTCCTTTCTTCCCTTTTCCTCAAGCTT
  TACAGGAAACCTACGTATGACAATTATGAGTTGCATCCCCGGAGCCACACAGGACAGAGGAGTCCTTCAG
  AAATCAGTGTTCACGTTGGAATAGATGACACCTCAAGAAATTCTCCAAGGCTGGGTTTGCTGGACCGGAT
  TGTCAACTATAGCAGAGCCTCCATAAATTCACTGCTGTCTGACAAGCGCTCCCTGAACAGCGTTGTTACT
  AGTGAACCTGACAAGCACTCGCTTCTGGTGGGCGACTTCCGGGAAGATGATGACACCACACTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009726
• Insert Size: 4476 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009726.5, NP_033856.3
• RefSeq Size: 8195 bp
• RefSeq ORF: 4476 bp
• Locus ID: 11977
• UniProt ID: Q64430
• Cytogenetics: X 47.36 cM
• Gene Summary: May supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.