Cdk12 (NM_001109626) Mouse Untagged Clone

CAT#: MC224546

Cdk12 (untagged) - Mouse cyclin-dependent kinase 12 (Cdk12), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cdk12
• Synonyms: 1810022J16Rik; AI646528; Crk7; Crkrs; D11Ertd752e; Pksc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224546 representing NM_001109626
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGCCCAATTCCGAGAGACATGGGGGCAAGAAGGACGGGAGCGGAGGAGCTTCTGGAACTTCGCAGCCGT
  CATCGGGAGGTGGCAGCTCCAACAGCAGGGAGCGTCACCGCTTGGTGTCGAAGCACAAGCGGCATAAGTC
  CAAGCACTCCAAAGACGTGGGGCTGGTGACCCCCGAAGCGGCATCTTTGGGTACCATAATCAAACCACTG
  GTGGAGTACGATGACATCAGCTCTGATTCAGACACCTTCTCCGATGACACGGCCTTCAAATCAGACCGGA
  GGGAGAACGAGGAACGTCGGGGAACGGATCGGAGCGATCGCCTGCACCGACATCGTCACCACCAGCACCG
  GCGGTCCCGAGACTTGCTAAAAACTAAACAGACGGAAAAGGAAAAAAATCAGGAAGTCTCCAAATCTGGA
  TCTATGAAGGACCGGGTATCGGGCAGTTCCAAACGGTCCGTGGAGGGGAGTGATGATTATGGGAAGGCCC
  AGCTATCCAAAAGCGGCAGCAAGGAATCCAGGTCGTCCAAAATGCACAAGGAGAAGACCCGCAAAGAGCG
  AGAGTTAAAGTCTGGATACAAGGACCGGAGTAAAAGTCATCGGAAAAGGGAAACACCCAAAAGTTACAAA
  ACCGTGGCTAGCCCTAAACGGAGATCCAGGAGTCCCCATAGGAAATGGTCTGACAGTTCCAAGCAAGATG
  ACAGCCCTTCCGGAGCTTCTTATGGCCAAGACTACGATCTTAGCCCCCCAAGGTCTCACACTTCTAGCAA
  CTATGACTCCTACAAGAAGAGTCCTGGAAGTACCTCAAGAAGGCAGTCAATCAGCCCACCTTACAAAGAG
  CCTTCTGCTTACCAGTCCAGCACTCGGTCACCCAGTCCTTACAGCCGACGACAGAGGTCTGTGAGTCCCT
  ATAGCCGGAGACGGTCCTCCAGCTATGAAAGGAGCGGCTCTTACAGCGGGAGATCACCCAGCCCCTATGG
  CCGAAGGCGATCAAGCAGCCCTTTCCTGAGCAAGAGGTCTCTGAGTCGGAGTCCACTCCCCAGTAGGAAA
  TCCATGAAGTCCAGAAGTAGAAGTCCTGCATATTCAAGACACTCATCTTCTCATAGTAAAAAGAAGCGAT
  CCGGGTCACGCAGTCGTCATTCCAGTATCTCACCTGTCAGGCTTCCATTGAATTCCAGCCTGGGAGCTGA
  ACTCAGTAGAAAAAAGAAGGAAAGAGCAGCAGCTGCTGCAGCAGCAAAAATGGATGGAAAAGAGTCCAAG
  AGTTCACCTATAATTTTGCCTAAAAAAGAGAAACTTGAGGTGAAGGAGTCAGGGTTAGAGTCTAAAAAGT
  TACCCAGAAGTATAAAATCAGAAAAATCGACCCCAGATACTGAACTGGTTACTGTAGCACATTCAAACCC
  AGAGGTAAAACATTGTTTAGACACAGGGAAGGTTAGGTTGGATGAGAACTTGCAGAAGCATCCTGCTAAG
  GATTTGAAAGCACAGGGAACAAAGGACGTTAAACCTGTAGCACCGAAAGAGGTGATTGTTACTTCAAAGG
  AGACAGAGACATCAGAAAAGGAGACCCTTCCACCTCTTCCCACAATTACTTCTCCACCCCCTTTACCAGC
  TACTACCCCTCCACCTCAGACACCCCCTTTGCCACCTTTGCCTCCACTACCAGCTATTCCGCTGCAGCCA
  CCTCTGCCTCCTCCCCAACCACCATTTAGTCAAGTTCCTGTTTCAAGTACTTCAATTTTACCCTCTTCTC
  CTCACCCAAGGACATCTACTCTATCCTCTCAGACAAATTCTCAGCCCCCTGTACAGGTTTCTATGAAGAC
  TCAAGTATCTATAACAGCTGCTATTCCACATCTGAAGACTTCAACATTGCCTCCTCTGCCCCTCCCTCCC
  CTATTACCTGGAGATGATGACATGGATAGTCCAAAAGAAACACTTCCTTCAAAGCCTGCAAAGAAAGAGA
  AGGAACAGAGGACTCGCCACTTGCTTACAGACTTGCCTCTTCCTCCTGAGCTACCAGGAGGAGATCCATC
  GCCTCCAGATTCTCCAGAGCCAAAGGCAATTACACCACCTCAACAACCATATAAAAAGAGACCAAAAATT
  TGTTGTCCACGTTATGGAGAAAGAAGACAAACAGAAAGTGATTGGGGGAAGCGCTGTGTGGACAAGTTTG
  ACATTATTGGGATTATTGGAGAGGGGACCTATGGCCAAGTATATAAAGCCAAGGACAAAGACACAGGAGA
  ACTAGTAGCTCTGAAGAAGGTTCGGCTGGACAATGAGAAAGAGGGCTTCCCAATCACAGCCATCCGGGAG
  ATCAAGATTCTTCGTCAATTAGTTCACCAGAGTGTTGTAAACATGAAGGAAATTGTCACAGACAAACAAG
  ATGCACTGGATTTCAAGAAGGACAAAGGTGCCTTTTACCTTGTATTTGAATATATGGACCATGACTTAAT
  GGGACTGCTTGAATCAGGTTTGGTGCACTTTTCTGAGGACCATATCAAGTCATTTATGAAACAGCTAATG
  GAAGGACTGGATTACTGTCACAAAAAGAATTTCCTCCATCGGGATATTAAATGTTCTAACATTTTGCTGA
  ATAACAGCGGGCAAATCAAACTGGCAGATTTTGGACTTGCTCGGCTCTATAACTCTGAAGAGAGTCGCCC
  TTACACAAACAAAGTCATCACTCTTTGGTATCGACCTCCAGAGCTTCTTCTTGGAGAGGAAAGATACACA
  CCAGCCATTGATGTTTGGAGCTGTGGGTGCATCCTTGGAGAACTGTTCACAAAGAAACCTATTTTTCAAG
  CCAATTTAGAACTGGCTCAGCTAGAACTTATCAGTCGTCTCTGTGGTAGTCCTTGTCCAGCAGTGTGGCC
  TGATGTTATCAAGCTGCCCTACTTCAACACCATGAAACCGAAGAAGCAATACAGGAGACGCCTAAGAGAA
  GAATTCTCTTTCATTCCTTCAGCGGCACTTGATCTATTGGACCACATGCTGACACTGGATCCTAGCAAGA
  GGTGCACAGCTGAACAGACCCTACAGAGTGACTTTCTTAAAGATGTGGAACTCAGCAAAATGGCACCTCC
  AGACCTACCTCACTGGCAGGATTGCCATGAATTGTGGAGTAAGAAACGTCGACGGCAGCGACAGAGTGGT
  ATTGTGATAGAAGATCCACCTCCGTCCAAAGCTTCTAGAAAAGAAACTACCTCAGGGACAACAGCTGAGC
  CTGTGAAAAACAATAGCCCAGCACCACCTCAGCCTGCTCCTGTCAAGGCAGAGCCTGGTCCAGGGGATGC
  AGTAGGCCTTGGTGACATCACACAGCAGTTGAATCAAAGTGAATTGGCAGTGTTATTAAACCTGCTTCAG
  AGCCAAACTGACCTGAGCATCCCTCAGATGGCACAGCTGCTTAATATCCACTCCAATCCAGAGATGCAAC
  AGCAGCTTGAAGCCTTGAATCAGTCTATTAGTGCACTGACTGAAGCCAGTTCCCAGCAGCAGGACTCAGA
  ATCCATAGCCCCTGAAGAATCATTGAAGGAGGTACCTTCTGTACCTGTGGTCCTGCCTCCTGCTGAACAG
  ACAACTCCTGAAGCTTCAAACACACCAGCTGACATGCAGAATGTGTTGGCAGTTCTCTTGAGTCAGCTGA
  TGAAAACCCAAGAGCCAGCAGGTAACCTGGAGGAAAACACCAATGACAAGAATAGTGGGCCACAGGGGCC
  CCGAAGAACTCCTACAATGCCACAGGAGGAGGCAGCAGCATGTCCTCCTCACATTCTTCCACCAGAGAAG
  AGGCCCCCTGAGCCCCCTGGACCTCCACCGCCGCCACCTCCACCCCCTCTGGTTGAAGGCGATCTTTCCA
  GCGCCCCCCAGGAGTTGAATCCCGCCGTGACAGCCGCCTTGCTGCAACTTTTATCCCAGCCTGAAGCAGA
  GCCTCCTGGCCACCTGCCACATGAGCACCAGGCCTTGAGACCAATGGAATACTCCACCCGATCCCATCCA
  AACAGGACTTACGGAAATACTGATGGGCCTGAGACAGGGTTCAGTTCCGCTGACACTGATGAACGCAGTT
  CTGGTCCAGCCTTGACAGAATCTTTGGTTCAGACCCCGGTGAAGAACAGGACCTTCTCAGGCTCTGTGAG
  CCACCTTGGGGAGTCCAACAGCTACCAGGGCACAGGGTCAGTGCAGTTCCCAGGGGACCAGGACCTCCGT
  TTTACCAGGGTTCCCTTAGCATTACACTCAGTGGTTGGGCAACCATTCCTCAAGTCTGAGGGAAATAGCA
  ACTCTGTGGTACATGCAGAGACCAAATTGCAAAACTATGGGGAGCTGGGACCGGGAACCACTGGGGCCAA
  CAGCTCAGGAACAACGCTTCAGTGGGGGGGCCCAGCTCAGTCTTATGGAAAACCCTACAGGGGGGCTGCA
  AGAGTCCTACCACGAGGGGGAAGAGGGAGAGGAGTTCCTTATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: AscI-MluI
• ACCN: NM_001109626
• Insert Size: 4455 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109626.1, NP_001103096.1
• RefSeq Size: 7669 bp
• RefSeq ORF: 4455 bp
• Locus ID: 69131
• UniProt ID: Q14AX6
• Cytogenetics: 11 61.75 cM
• Gene Summary: Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.[UniProtKB/Swiss-Prot Function]