Mug1 (NM_008645) Mouse Untagged Clone

CAT#: MC224526

Mug1 (untagged) - Mouse murinoglobulin 1 (Mug1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mug1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224526 representing NM_008645
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAAGAGCAGACGGGCTCAGTTGTGCCTTTTTTCGGTTCTCCTTGCCTTCCTGCATTCTGCTTCTT
  TACTCAACGGAGACTCGAAGTATATGGTGCTGGTCCCTTCTCAGCTCTACACTGAGACCCCTGAGAAAAT
  CTGCCTCCATCTATACCAGCTGAATGAAACAGTGACCGTCACAGCTTCCTTGGTATCTCAATCAGGAAGG
  AAAAACCTGTTCGATGAGCTGGTGCTTGACAAGGACTTGTTCCAATGTGTTTCCTTCATTATCCCTAGAC
  TCTCCTCTTCTGATGAGGAGGATTTTCTCTACGTGGACATCAAAGGGCCAACCCACGAATTCAGCAAAAG
  GAAAGCAGTGCTTGTGAAGAACAAAGAAAGTGTTGTCTTTGTGCAGACAGATAAGCCCGTGTACAAGCCA
  GGACAATCAGTTAAATTTCGGGTTGTCTCTATGGATAAAATGCTACGTCCCCTGAATGAGTTGCTTCCTC
  TGGCTTACATTGAGGATCCGAAAAAGAACCGAATTATGCAGTGGAGGGATATTAAGACTGAGAATGGGCT
  TAAGCAAATGTCCTTCAGCCTGGCAGCAGAGCCCATTCAGGGCCCCTACAAGATAGTGGTGCACAAAGAG
  TCAGGGGAGAAGGAAGAACACTCCTTTACTGTGATGGAATTTGTGCTTCCCAGATTTAATGTCGACCTGA
  AGGTCCCAAATGCCATGTCTGTGAATGATGAAGTGCTCAGTGTGACTGCATGTGGGAAATATACCTATGG
  GAAGCCTGTTCCAGGACATGTGAAGATAAACGTCTGCCGTGAGACTGAGACTGGATGCAGAGAAGTCAAT
  TCTCAGCTAGACAACAATGGCTGCAGTACACAAGAAGTGAACATCACTGAGCTCCAATCAAAGAAAAGGA
  ATTATGAGGTTCAGCTTTTCCATGTGAATGCAACTGTTACAGAAGAAGGGACAGGATTGGAGTTCAGTCG
  ATCTGGAACAACTAAAATTGAAAGAATCACAAACAAGCTCATATTTCTGAAGGCAGATTCACACTTCAGA
  CATGGAATTCCATTCTTTGTGAAAGTCCGCCTAGTGGATATCAAGGGAGACCCTATCCCAAATGAGAAAG
  TCTTCATCAAAGCACAAGAACTTAGCTATACCAGTGCTACTACCACTGATCAGCATGGCCTGGCAGAGTT
  CTCCATAGATACCACATGCATCTCGGGCTCTTCCCTCCATATCAAAGTCAACCACAAAGAGGAAGATTCA
  TGTTCCTATTTCTATTGCATGGAGGAAAGACATGCAAGTGCAAAGCATGTGGCCTATGCTGTTTACTCCC
  TCAGCAAGAGCTACATCTACCTTGACACAGAGACCAGCAGCATCTTGCCCTGCAACCAGATTCACACAGT
  TCAGGCACATTTTATTCTGAAGGGGGACTTGGGAGTGCTGAAAGAGCTCATTTTCTACTACCTGGTCATG
  GCGCAGGGAAGCATCATCCAGACTGGAAACCATACTCACCAGGTGGAGCCAGGAGAAGCTCCAGTAAAAG
  GAAAATTTGCCTTGGAGATCCCTGTGGAGTTTAGCATGGTCCCCATGGCTAAAATGCTCATCTACACGAT
  CTTGCCTGATGGAGAAGTGATTGCAGATTCTGTAAACTTTGAAATTGAAAAGTGTCTTCGCAACAAAGTG
  GACCTGCGCTTCAGCACTTCTCAAAGTCTTCCCGCCTCACAAACCCGTCTGCAGGTCACAGCTTCTCCTC
  AGTCCCTCTGTGGCCTGAGAGCTGTGGACCAGAGTGTGCTACTCCTGAAACCCGAGTCTGAGCTCTCCCC
  TTCCTGGATATACAATCTGCCAGGTATGCAGCAAAACAAATTCGTTCCAAGTTCCCGTCTGTCTGAAGAC
  CAAGAAGACTGTATACTGTACAGTTCATGGTTGGCTGAGAAGCACACAAACTTAGTACCACATGGAACTG
  AGAAGGATGTCTATAGATATGTGGAGGACATGGGTTTAACAGCATTCACCAACTTGATGATCAAACTTCC
  TATAATTTGTTTTGACTATGGCATGGTTCCAATATCAGCACCTCGTGTGGAATTTGATCTTGCATTTACC
  CCTGAAATTTCTTGGAGTTTACGCACAACATTGAGTAAAAGACCTGAAGAGCCACCACGTAAAGATCCAT
  CATCGAATGATCCACTTACAGAGACCATTCGAAAATATTTTCCTGAAACCTGGGTCTGGGATATAGTCAC
  AGTAAACTCCACAGGACTGGCTGAAGTGGAAATGACTGTCCCTGACACCATCACTGAATGGAAGGCAGGA
  GCTCTCTGCTTGTCCAATGACACTGGCCTTGGCCTCTCTTCTGTGGTACCTCTCCAAGCCTTCAAGCCCT
  TCTTTGTGGAGGTCTCATTGCCCTATTCTGTGGTCCGTGGAGAAGCCTTCATGCTCAAGGCCACTGTGAT
  GAACTATCTCCCCACAAGCATGCAGATGAGTGTGCAGCTGGAAGCCTCTCCTGATTTCACAGCTGTCCCA
  GTGGGAGACGACCAAGATTCTTACTGCCTCAGTGCCAATGGGAGGCACACCTCATCCTGGCTGGTAACTC
  CCAAGTCTTTAGGGAATGTGAATTTCTCTGTGTCTGCGGAAGCACAACAGTCCTCAGAGCCCTGTGGTTC
  TGAGGTGGCCACAGTTCCTGAAACTGGGAGAAAGGACACAGTAGTCAAAGTCCTGATAGTTGAGCCTGAA
  GGAATCAAGCAAGAGCATACCTTCAGCTCACTGTTCTGTGCATCAGATGCTGAGATATCTGAAAAAATGT
  CCCTGGTCCTCCCACCAACAGTAGTGAAAGATTCAGCAAGAGCCCATTTCTCTGTGATGGGTGATATCTT
  GAGTTCAGCCATAAGAAACACACAAAACCTTCTCCATATGCCCTATGGCTGTGGGGAGCAGAACATGGTC
  CTTTTTGCTCCCAACATCTACGTACTGAAATATCTGAATGAAACCCAACAGCTGACTCAGAAGATCAAGA
  CTAAAGCCCTTGGGTTCCTCAGAGCTGGTTATCAGAGAGAGCTGAACTACAAACACAAGGATGGCTCCTA
  CAGTGCCTTCGGGGATCAAAATGGCGAAAGAGAAGGAAACACTTGGCTCACAGCCTTTGTGCTCAAGTCT
  TTTGCCCAAGCTCGAGCCTTCATCTTCATCGATGAATCACACATCACCCATGCCTTCACCTGGCTCTCCC
  AAAAGCAGAAGGACAATGGCTGCTTCCGGAGCTCCGGATCATTGTTCAACAATGCCATGAAGGGGGGAGT
  AGATGATGAAATGACCCTCTCTGCCTACATAACCATGGCCCTTCTGGAAAGTTCACTCCCAGCCACGCAT
  CCTGTTGTCTCCAAAGCCCTGAGCTGCCTGGAGTCATCCTGGAAGACCATAGAACAAGAACGAAATGCCA
  GCTTTGTGTACACCAAGGCTTTGATGGCCTATGCTTTTGCTCTGGCGGGGAACCAGAATAAGAGAGATGA
  AATCCTGAAGTCACTTGATGAGGAAGCTATAAAAGAAAACAACTCTATCCACTGGAAAAGACCTCAGAAA
  TCCAGGAAATCTGAACACCATTTGTACAAACCCCAGGCTTCCTCTGCTGAAGTAGAGATGAATGCATATG
  TGGTCCTGGCTCGCCTCACTGCCCAGCCAGCCCCATCCCCTGAGGATCTGACTTTGTCAATGAGCACCAT
  CATGTGGCTCACAAAGCAACAGAATTCCAATGGTGGCTTCTCCTCCACACAGGACACTGTGGTGGCCCTT
  GATGCTCTGTCCAAATATGGAGCAGTGACTTTTTCGAGAAGTCAGAAAACGACTTTGGTGACTATCCAAT
  CTACAGGGTCATTTTCCCAAAAGTTCCAAGTGGAGAACAGTAATCGCCTGTTACTACAGCAGGTCGCATT
  ACCAGACATTCCTGGGGACTATACCATCAGTGTGTCAGGGGAAGGATGTGTGTATGCTCAGACTATGCTG
  AGATACAACATGCACTTGGAGAAGCAGCTGTCTGCATTTGCTATATGGGTACAGACAGTACCTCTAACTT
  GTAATAACCCCAAAGGCCACAACAGCTTCCAGATCTCACTAGAAATCAGTTACACAGGCAGCCGGCCAGC
  CTCCAACATGGTGATTGCTGATGTGAAGATGCTATCTGGTTTCATCCCATTGAAACCAACAGTGAAAAAG
  CTTGAAAGATTAGAGCACGTGAGCAGAACAGAAGTGAGCAACAACAATGTCTTAATATATTTGGATCAGG
  TGACCAATCAGACACTGGCCTTCTCCTTCATCATTCAACAAGATATCCCAGTAAGGAACCTGCAGCCTGC
  CATTGTGAAAGTCTATGACTACTATGAGACAGATGAAATGGCTTTTGCTGAATACAGCAGCCCCTGCAGC
  ACAGACAAACAAAATGTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008645
• Insert Size: 4431 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008645.3, NP_032671.2
• RefSeq Size: 4657 bp
• RefSeq ORF: 4431 bp
• Locus ID: 17836
• UniProt ID: P28665
• Cytogenetics: 6 F1
• Gene Summary: A proteinase activates the inhibitor by specific proteolysis in the bait region, which, by an unknown mechanism leads to reaction at the cysteinyl-glutamyl internal thiol ester site and to a conformational change, whereby the proteinase is trapped and/or covalently bound to the inhibitor. While in the tetrameric proteinase inhibitors steric inhibition is sufficiently strong, monomeric forms need a covalent linkage between the activated glutamyl residue of the original thiol ester and a terminal amino group of a lysine or another nucleophilic group on the proteinase, for inhibition to be effective.[UniProtKB/Swiss-Prot Function]