Nos1 (NM_008712) Mouse Untagged Clone

CAT#: MC224449

Nos1 (untagged) - Mouse nitric oxide synthase 1, neuronal (Nos1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nos1
• Synonyms: 2310005C01Rik; bNOS; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224449 representing NM_008712
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGCACACGTTTGGGGTCCAGCAGATCCAACCCAACGTCATTTCTGTCCGTCTCTTCAAACGCA
  AAGTGGGAGGTCTGGGCTTCCTGGTGAAGGAACGGGTCAGCAAGCCCCCGGTGATCATCTCAGATCTGAT
  CCGAGGAGGTGCCGCGGAGCAGAGCGGCCTTATCCAAGCCGGCGACATCATTCTCGCCGTCAATGATCGG
  CCCCTGGTAGACCTCAGCTATGACAGCGCTCTGGAAGTTCTCAGGGGCATTGCCTCTGAGACCCATGTGG
  TCCTCATTCTGAGAGGCCCTGAGGGCTTTACTACACACCTGGAGACCACCTTCACAGGGGATGGAACCCC
  CAAGACCATCCGTGTGACCCAGCCCCTGGGGACACCCACCAAAGCTGTCGATCTGTCTCGCCAGCCATCA
  GCCAGCAAAGACCAGCCATTAGCAGTAGACAGGGTCCCAGGTCCCAGTAACGGACCTCAGCATGCCCAAG
  GCCGTGGACAGGGAGCCGGCTCAGTCTCCCAGGCTAATGGTGTGGCCATTGACCCCACAATGAAAAATAC
  CAAGGCCAACCTCCAGGACAGCGGAGAACAGGATGAACTGCTCAAAGAGATAGAACCTGTGTTGAGCATC
  CTCACCGGTGGGGGAAAAGCAGTCAACAGAGGGGGACCAGCCAAAGCAGAGATGAAAGACACAGGAATCC
  AGGTGGACAGAGACCTCGACGGCAAACTGCACAAAGCTCCGCCCCTGGGCGGGGAGAATGATCGAGTCTT
  CAATGATCTGTGGGGGAAGGGCAACGTCCCTGTGGTCCTCAACAACCCGTATTCAGAGAACGAACAGTCT
  CCCGCCTCGGGCAAACAGTCTCCTACCAAGAATGGCAGTCCTTCCAGGTGCCCTCGCTTCCTTAAGGTCA
  AGAACTGGGAGACAGATGTGGTCCTCACTGACACCCTGCACCTGAAGAGCACGCTGGAAACGGGGTGCAC
  AGAGCAGATCTGCATGGGCTCCATCATGCTGCCATCCCATCACATACGGAAGTCAGAAGATGTCCGCACC
  AAGGACCAGCTCTTCCCTCTAGCCAAAGAATTTCTCGACCAATACTACTCCTCCATTAAGAGGTTTGGCT
  CCAAGGCCCACATGGACAGGCTGGAGGAGGTGAACAAGGAGATCGAGAGCACCAGCACCTACCAGCTCAA
  GGACACGGAGCTCATCTATGGCGCCAAGCATGCCTGGCGGAACGCCTCTCGCTGTGTGGGCAGGATCCAG
  TGGTCCAAGCTGCAGGTGTTCGATGCCCGAGACTGCACCACAGCCCATGGGATGTTCAACTACATCTGTA
  ACCACGTCAAGTACGCCACCAACAAAGGGAACCTCAGGTCGGCCATCACTATATTCCCTCAAAGGACTGA
  TGGCAAGCATGACTTCCGAGTGTGGAACTCGCAGCTCATCCGCTATGCCGGCTACAAGCAGCCAGATGGC
  TCTACCTTGGGCGATCCAGCTAATGTGGAGTTCACAGAGATCTGTATACAGCAGGGCTGGAAACCCCCAA
  GAGGCCGGTTCGATGTGCTGCCTCTCCTGCTCCAGGCCAATGGCAATGACCCTGAACTCTTTCAGATCCC
  CCCAGAGCTGGTGCTGGAAGTACCCATCAGGCACCCCAAGTTCGACTGGTTTAAGGACCTGGGGCTCAAA
  TGGTATGGCCTCCCCGCTGTGTCCAACATGCTGCTGGAGATTGGGGGCCTGGAGTTCAGCGCCTGTCCCT
  TTAGTGGCTGGTACATGGGCACGGAGATTGGCGTTCGTGATTACTGTGACAACTCTCGATACAACATCCT
  GGAGGAAGTAGCCAAGAAAATGGATTTGGACATGAGGAAGACATCCTCCCTCTGGAAGGACCAAGCCCTG
  GTGGAGATTAACATTGCTGTCCTATACAGCTTCCAGAGTGACAAGGTGACCATTGTGGACCACCACTCTG
  CCACCGAGTCCTTCATCAAACACATGGAGAATGAGTACCGCTGCAGAGGGGGCTGTCCCGCCGACTGGGT
  GTGGATTGTGCCCCCCATGTCGGGCAGCATCACCCCTGTCTTCCACCAGGAGATGCTCAACTACCGGCTC
  ACCCCGTCCTTTGAGTACCAGCCTGATCCATGGAACACCCACGTGTGGAAGGGCACCAATGGGACCCCCA
  CGAAGCGGCGAGCCATTGGCTTCAAGAAATTGGCAGAGGCCGTCAAGTTCTCAGCCAAGCTGATGGGACA
  GGCCATGGCCAAGAGAGTCAAAGCGACCATTCTCTACGCCACCGAGACAGGCAAATCCCAAGCCTATGCC
  AAGACCCTGTGCGAGATCTTCAAGCATGCCTTCGATGCCAAGGCTATGTCCATGGAGGAATATGACATCG
  TGCACCTGGAGCACGAAGCCCTGGTCTTGGTGGTCACCAGCACCTTTGGCAATGGAGACCCCCCAGAGAA
  TGGGGAGAAATTCGGCTGTGCTTTGATGGAGATGAGGCACCCCAACTCTGTGCAAGAGGAGAGGAAGAGC
  TACAAGGTCCGATTCAACAGCGTCTCCTCCTATTCTGACTCCCGCAAGTCATCAGGCGATGGACCAGACC
  TCAGAGACAACTTTGAAAGTACTGGACCGCTGGCCAATGTGAGGTTCTCAGTCTTCGGCCTGGGCTCTCG
  GGCATACCCTCACTTCTGTGCCTTTGGGCACGCAGTGGACACTCTCCTGGAGGAGCTGGGCGGGGAGAGG
  ATTCTGAAGATGAGGGAGGGAGATGAGCTCTGCGGACAGGAAGAGGCTTTCAGGACCTGGGCTAAGAAAG
  TCTTCAAGGCAGCCTGTGATGTGTTCTGTGTGGGGGATGACGTCAACATTGAGAAGGCGAACAATTCCCT
  CATTAGCAATGACCGCAGCTGGAAGAGGAATAAATTCCGCCTCACTTATGTGGCAGAAGCTCCAGAGCTG
  ACCCAAGGTCTTTCCAATGTTCACAAAAAGCGAGTCTCAGCCGCCCGACTCCTCAGCCGCCAAAACCTGC
  AAAGTCCTAAATCCAGCCGATCGACCATCTTCGTGCGTCTCCACACCAACGGGAATCAGGAGTTGCAGTA
  CCAGCCAGGGGACCACCTGGGTGTCTTCCCTGGCAACCATGAGGACCTCGTGAATGCACTCATTGAACGG
  CTGGAGGACGCGCCGCCTGCCAACCACGTGGTGAAGGTGGAAATGCTGGAGGAAAGGAACACGGCTCTGG
  GTGTCATCAGTAATTGGAAGGATGAATCTCGCCTCCCACCCTGCACCATCTTCCAGGCCTTCAAGTACTA
  CCTGGACATCACCACGCCGCCCACGCCCCTGCAGCTGCAGCAGTTCGCCTCCCTGGCCACCAATGAGAAA
  GAGAAGCAGCGGCTGCTGGTCCTCAGCAAGGGGCTTCAGGAATATGAGGAATGGAAATGGGGCAAGAACC
  CCACAATGGTGGAGGTGCTGGAGGAGTTCCCGTCCATCCAGATGCCGGCCACACTTCTCCTCACACAGCT
  GTCGCTGCTACAACCTCGCTACTATTCCATCAGCTCCTCTCCAGACATGTACCCCGACGAGGTGCACCTC
  ACCGTGGCCATCGTGTCCTACCATACCCGAGATGGAGAAGGACCGGTTCACCACGGGGTGTGCTCCTCCT
  GGCTCAACCGAATACAGGCTGACGATGTCGTCCCCTGCTTCGTGAGAGGTGCCCCTAGCTTCCACCTGCC
  TCGAAACCCCCAGGTGCCTTGCATCCTGGTTGGCCCAGGCACCGGCATCGCTCCCTTCCGAAGTTTTTGG
  CAACAGCGACAATTTGACATCCAACACAAAGGAATGAATCCGTGCCCCATGGTTCTGGTCTTCGGGTGTC
  GACAATCCAAGATAGATCATATCTACAGAGAGGAGACCCTGCAGGCCAAGAACAAGGGCGTCTTCAGAGA
  GCTGTACACTGCCTACTCCCGGGAACCCGACAGGCCAAAGAAATATGTACAGGACGTGCTGCAGGAACAG
  CTGGCCGAGTCTGTGTACCGCGCCCTGAAGGAGCAAGGAGGCCATATTTATGTCTGCGGGGACGTTACCA
  TGGCCGCTGATGTCCTCAAAGCCATCCAGCGCATAATGACCCAGCAGGGGAAACTCTCGGAGGAGGATGC
  TGGTGTGTTCATCAGCAGGCTGAGGGATGACAACCGGTACCACGAGGACATCTTTGGGGTCACCCTCAGA
  ACATATGAAGTGACCAACCGCCTTAGATCTGAGTCCATCGCCTTCATTGAAGAGAGCAAAAAAGACACAG
  ATGAGGTTTTCAGCTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008712
• Insert Size: 4290 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008712.3, NP_032738.1
• RefSeq Size: 9695 bp
• RefSeq ORF: 4290 bp
• Locus ID: 18125
• UniProt ID: Q9Z0J4
• Cytogenetics: 5 57.29 cM
• Gene Summary: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Isoform NNOS Mu may be an effector enzyme for the dystrophin complex.[UniProtKB/Swiss-Prot Function]