Cgnl1 (NM_026599) Mouse Untagged Clone

CAT#: MC224188

Cgnl1 (untagged) - Mouse cingulin-like 1 (Cgnl1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cgnl1
• Synonyms: 4933421H10Rik; 9930020M10Rik; AI503810; Ja; Jacop
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224188 representing NM_026599
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGTATTTTGGCGAATACCAACACGTACAGCAGGAGTACGGGGTACACCTGAGACTTGCCAGTG
  GTGACACTCCAAAGCCAAGGAATTCCCAGCCCTCAAAGGCAGGCTCCTATGGTGTCAGCATCAGGGTCCA
  GGGAATTGATGGCCACCCTTATATAGTCCTGAATAACACTGAGCGGTGTCTAGCAGGCACACCTTTCCCA
  GAAAACGCGCCATCGTTTCCATCTTCAGTGATAAATAACCTGTCCCTACATCCAAGCAATGGAACCGTGT
  TGAAGGAGAACACTCCGGAGGAACTGCAGCTTCCAGAAAACCCGTACCTGCAAACCAGCCCGCTAAGAGG
  CCAGAAGCAGTTCTCCCTCCACGAGGGCAGGAATGGAGTTCTAGAACGCAAAGACGGGCCCACGAAGCTG
  CCCCATGTGCTCAACTTCCAGAGGCACCCGGAGCTTCTGCAGCCCTATGACCCAGAAAAGAATGAGGTGA
  ACGCAAAGAAGCATCACCCTCCCGAGAGTCCCTGGCTGAGAAATGCAACAGAGGACGGGACCAACTGTAA
  GAAGTCCCGGAACTGCTTCCCCAAATCCTACGGTTCCCAGCCCAACAGCCCTACTTCGGAAGATTTAGCC
  AAGACCAACATGACAGCGATCCGTCTCTGCAGCTCTGTGGTCATAGAAGACCCCCAAAAGCAGACCTCAG
  TGTGCGTGAACGTTCAGAGGTGCGCCAAGGAGGGGGTGGGAGAGGAGACCCTTTCCCCTCGACGGAAATC
  CCCAACTGCCCCCAGCCCACAGGCCTATTCTGAAACCAAGAAAAACAGGCCAGATGTGCTACCCTTCCGG
  CGACAAGATTCCGCAGGACCCATCCTGGATGGAGCTCGGTCACGCAGGTCCTCTTCGTCATCCACGACTC
  CTACTTCTGCCACTTCCTTGTACAAATTTTTACTTGATGATCAGGAATGCGCCATTCATGCCGACAGCGT
  CAACCGCCATGAAAACAGAAGGTATATTCCCTTCTTGCCAGGAACTGGGCGGGATATCGACACCTGCTCA
  ATTCCTGGTGTGGACCAGTTAATTGAAAAATTTGACCAAAAGCCTGGCCTTCAGAGAAGAGGAAGGTCTG
  GGAAGAGAAACAGGATCAATCCCGACGACAGGAAAAGATCCCGAAGTGTAGATAGTGCCTTTCCATTTGG
  TCTCCAGGGAAACACAGAATACCTCACCGAGTTCAGCAGGAACCTGGGCAAGTCTAGCGAACACCTCCTT
  CGGCCATCCCAGGTCTTCCCGCAGAGGTCAGTGGCCCAGGAGCACCGTGGGAAGCACAGTCCCAGCAGCC
  CGCCCGCAAAGCTGCAGGGCGCTCAAGGTGCCCACCCCAAGCCTCCCCTGCAGAACAAAGATGGGAAAGT
  TCTGAACAAAGGAAGGCAGGAAAGCACGGGGGCATGTGCCCCCTCCCTGCCAGCACCGAATAAAAAAGAG
  GAAGAAATCAAAATAGCCACTGCCACGTTGATGTTACAGAACCGGGCGGTGGCTGCCACATCGGATTCTG
  GTGCCAAGAAAATTTCAGTGAAGACATTTCCTTCCGACTCCAGCACTCAGGCCACACCAGATCTCTTAAA
  GGGCCAGCAGGAGCTCACACAACAAACGAACGAGGAGACCGCCAAGCAGATCCTTTACAATTACCTCAAA
  GAAGGAGGCACAGACAATGAGGATGCCACCAAAAGGAAAGTGAACCTGGTCTTTGAGAAAATCCAGACTT
  TGAAGTCCAGAGCAGCAGGGAGTGCCCAAGGAAGCAATCAAGCTCCGAATTCTCCCTCTGAAGGCAACAG
  TCTATTGGATCAGAAGAACAAATTGATCCTAGAAGTGTCTGAACTACAGCAGCAACTGCAACTGGAGATG
  AAGAACCAACAGAACATCAAGGAGGAGCGAGAAAGAATGAGAGAGGATTTAGAAGAGCTGCGAGTCCGGC
  ACCAGAGCCAAGTGGAGGAGACGGCCACCCTGCAGAGACGGCTGGAGGAAAGTGAAGGCGAGCTGAGGAA
  GAGCTTGGAAGAGCTGTTCCAAGTGAAGATGGAGCGTGAACAGCACCAGACTGAGATCAGAGACCTGCAG
  GACCAGCTGTCGGAAATGCATGATGAGCTAGACAGCACCAAGCGATCCGAGGACAGGGAGAAGGGAGCCC
  TGATTGAGGAGCTCCTGCAAGCCAAGCAGGACCTTCAAGATCTGCTCATTGCCAAAGAGGAACAGGAAGA
  TCTCTTGAGGAAGCGGGAGCGGGAGCTGACTGCCTTGAAGGGCGCCTTGAAGGAGGAGGTTTCCAGCCAT
  GACCAGGAGATGGATAAGCTGAAGGAACAGTACGATGCTGAGCTGCAGGCCCTCAGGGAGAGTGTGGAGG
  AGGCAACCAAGAATGTTGAGGTCCTGGCCAGCAGGAGCAACTCCTCAGAGCAGAGCCAGGCGGAGGCTGA
  CCTGCGTGAGAAAGTGCTGAAGGAGGAGAATGAGAAGCTGCAGGGGAGGATTGCGGAGCTGGAGCGCAGG
  GCAGCCCAGCTACAGAGGCAGATGGAGGATGTGAAGGGTGACGAGGCCCAAGCAAAGGAGACACTCCGAA
  AGTGTGAGAGTGAAGTACAGCAGCTGGAGGAAGCCCTTGTGCATGCCAGAAAGGAAGAAAAAGAAGCCAC
  ATGTGCCAGAAGGGCGCTGGAGAAGGAGCTGGAGCAAGCTCGGAGAGAGCTGAGCCAGGTCTCCCAGGAG
  CAGAAGGAACTCCTGGAGAAACTCCGGGACGAGGCGGAGCAGAAGGAGCAGCTGCGAAAGCTGAAGAACG
  AGATGGAGAGCGAGCGGTGGCACCTGGACAAGACCATCGAGAAGCTGCAGAAGGAGATGGCCGACATTGC
  TGAGGCCTCCCGCACATCCTCTTTGGAGCTGCAGAAGCAGCTGGGCGAGTATAAGGAGAAGAACCGCCGG
  GAGCTTGCGGAAATGCAGACACAACTGAAGGAGAAATGCCTGGAAGTGGAAAAGGCCAGACTGGCAGCCT
  CGAAGATGCAGGATGAGTTACGCCTCAAGGAGGAAGAGTTGCAGGACTACCAGCGAGCTGAGGAGGAGGC
  GCTCACAAAACGGCAGCTTCTGGAGCAGTCGCTGAAGGACCTGGAGTATGAGCTGGAGGCCAAGAGCCAC
  CTCAAGGATGACCGCAGCCGACTCATCAAGCAGATGGAGGACAAGGTGTCCCAATTGGAGATTGAATTGG
  AAGAAGAAAGAACCAACGCTGACTTGCTGTCGGAGAGGATCACGTGGAGCAGGGAACAGATGGAGCAGAT
  GAGGAGCGAGCTTCTTCAGGAGAAAGCTGCCAAGCAAGACTTAGAGTGTGACAAGATTTCCCTAGAGAGA
  CAGAACAAAGACTTAAAGAGCCGGATTATCCACCTGGAAGGTTCCTACAGGTCCAGCAAGGAGGGCCTGG
  TGGTGCAGATGGAGGCCAGGATTGCAGAGCTGGAGGACCGGCTGGAGAATGAGGAGAGGGATCGGGCCAA
  CCTGCAGCTCAGCAACCGAAGGCTGGAGCGGAAGGTGAAGGAGCTGGTGATGCAGGTGGATGATGAGCAT
  CTGTCACTGACGGACCAGAAGGACCAGCTGAGCTTGCGCCTGAAAGCCATGAAGCGCCAGGTGGAGGAGG
  CTGAAGAGGAGATCGACCGACTGGAAAGTTCTAAGAAGAAGCTGCAGAGGGAGCTGGAGGAGCAGATGGG
  GGTGAATGAGCAGCTGCAGGGGCAGCTCAACTCTCTGAAGAAGGGCTTAAGGCTTAAGACACTGTCTAGT
  AAAGTACTGGACGACTCGGACGACGACGACCTCAGCAGTGATGCGGGAAGCCTCTACGAAGCCCCACTGA
  GCTATGCCTTTCCCAAAGACAGCACTATCGCCAGCCAGATCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_026599
• Insert Size: 3894 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026599.5, NP_080875.3
• RefSeq Size: 6781 bp
• RefSeq ORF: 3894 bp
• Locus ID: 68178
• UniProt ID: Q6AW69
• Cytogenetics: 9 D
• Gene Summary: This gene encodes a protein localized to the tight junctions and adherens junctions in vertebrate epithelial cells. The encoded protein regulates the activity of Rho family GTPases during junction assembly and at confluence. At the adherens junctions, the encoded protein is part of a protein complex that links E-cadherin to the microtubule cytoskeleton. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein.