Plcg1 (NM_021280) Mouse Untagged Clone

CAT#: MC224181

Plcg1 (untagged) - Mouse phospholipase C, gamma 1 (Plcg1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plcg1
• Synonyms: AI894140; Cded; Plc-1; Plc-gamma1; Plcg-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224181 representing NM_021280
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGTCGCGACCCCCTGCGCCAACGGCTGCGGGCCTGGCGCACCCTCCGAAGCCGAGGTGCTGC
  ACCTCTGCCGCAGCCTCGAGGTGGGCACCGTCATGACTTTGTTCTACTCCAAGAAGTCGCAGCGGCCAGA
  ACGGAAGACCTTCCAGGTCAAGTTGGAGACGCGCCAGATCACATGGAGCCGCGGCGCGGACAAAATCGAG
  GGGTCCATTGATATCCGAGAAATCAAGGAGATCCGTCCAGGGAAGACTTCTCGGGACTTTGACCGCTACC
  AAGAAGACCCTGCCTTCCGGCCAGATCAATCACACTGCTTTGTCATCCTCTATGGAATGGAATTCCGCTT
  GAAGACCCTGAGCCTGCAAGCCACATCTGAGGATGAAGTGAACATGTGGATCAAGGGCTTAACTTGGCTA
  ATGGAGGATACACTGCAGGCAGCCACACCCCTGCAAATTGAGAGGTGGCTCCGGAAGCAGTTCTACTCAG
  TGGATCGTAACCGAGAGGATCGTATATCAGCCAAGGACTTGAAGAACATGCTGTCTCAGGTCAACTACCG
  AGTCCCCAATATGCGCTTCCTCCGAGAGCGGCTGACGGACCTTGAACAGCGCAGCGGGGACATCACCTAC
  GGGCAGTTTGCTCAGCTGTACCGCAGCCTCATGTACAGCGCCCAGAAGACGATGGACCTTCCCTTCTTGG
  AAACCAACGCTTTGAGGACTGGAGAGCGTCCGGAGCATTGCCAGGTGTCCCTTTCTGAGTTCCAGCAGTT
  CCTCCTTGAATACCAGGGGGAGCTGTGGGCTGTCGACCGGCTCCAGGTGCAGGAATTCATGCTCAGCTTC
  CTTCGAGACCCCTTGCGAGAGATTGAGGAGCCATACTTCTTCTTGGATGAGCTTGTCACCTTCCTGTTCT
  CCAAAGAGAACAGCGTGTGGAACTCACAGCTGGATGCTGTGTGCCCGGACACCATGAACAACCCACTCTC
  TCACTATTGGATCTCCTCCTCACATAATACGTACCTGACTGGGGACCAGTTCTCCAGTGAGTCCTCCCTG
  GAAGCCTATGCTCGCTGCCTGAGGATGGGCTGTCGCTGCATTGAGTTGGACTGCTGGGATGGGCCAGATG
  GGATGCCAGTCATTTACCATGGGCACACTCTCACCACCAAGATCAAGTTCTCTGACGTCCTGCACACCAT
  CAAGGAGCATGCTTTCGTAGCCTCAGAGTACCCAGTCATCCTGTCCATCGAGGACCACTGCAGCATTGCC
  CAGCAGAGAAACATGGCTCAGCACTTCAGAAAGGTGCTTGGCGACACACTCCTTACCAAGCCTGTGGACA
  TTGCTGCTGATGGCCTTCCTTCTCCCAACCAGCTCAGGAGGAAGATCCTTATTAAGCACAAGAAGCTGGC
  TGAGGGCAGTGCCTATGAGGAGGTGCCTACCTCTGTGATGTACTCCGAGAATGACATCAGTAACTCCATC
  AAGAATGGTATCCTCTACTTGGAGGACCCCGTGAATCATGAGTGGTATCCCCACTACTTCGTTCTGACTA
  GCAGCAAGATCTACTACTCTGAGGAGACCAGCAGTGACCAGGGCAATGAGGATGAAGAGGAGCCGAAGGA
  GGCCAGCAGCAGCACAGAGCTGCACTCGAGTGAGAAGTGGTTCCACGGGAAGCTCGGGGCTGGACGTGAC
  GGGCGGCACATTGCTGAGCGCCTGCTCACTGAGTACTGCATAGAGACTGGGGCTCCTGATGGCTCCTTCC
  TAGTGCGAGAAAGTGAGACCTTCGTGGGTGACTACACGCTGTCTTTCTGGCGGAATGGGAAAGTCCAGCA
  CTGCCGCATCCACTCCCGGCAGGATGCTGGGACTCCTAAGTTCTTCTTGACAGATAACCTTGTCTTTGAC
  TCTCTCTATGACCTCATCACACATTATCAGCAAGTACCCCTGCGCTGCAATGAGTTTGAGATGCGCCTTT
  CAGAGCCTGTTCCACAGACGAATGCCCATGAGAGCAAAGAGTGGTACCACGCAAGCCTGACTAGAGCTCA
  GGCTGAACATATGCTGATGCGAGTGCCCCGGGATGGGGCCTTCCTGGTGCGGAAACGCAATGAGCCTAAC
  TCATATGCCATCTCTTTCCGGGCTGAGGGAAAGATCAAGCACTGCCGAGTACAGCAGGAAGGCCAGACAG
  TGATGCTGGGGAACTCTGAGTTTGACAGCCTGGTTGACCTCATCAGCTACTATGAGAAGCACCCCCTGTA
  CCGCAAAATGAAGCTACGCTACCCCATCAACGAGGAGGCACTGGAGAAGATCGGGACAGCTGAACCCGAT
  TATGGGGCACTATACGAGGGCCGCAACCCTGGTTTCTATGTGGAGGCAAACCCTATGCCAACTTTCAAGT
  GTGCAGTAAAAGCCCTCTTCGACTACAAGGCCCAGAGAGAGGATGAGCTGACCTTCACCAAGAGTGCCAT
  CATCCAGAATGTGGAAAAGCAAGATGGTGGCTGGTGGCGAGGGGACTATGGTGGGAAGAAGCAGCTGTGG
  TTCCCCTCAAACTATGTGGAAGAGATGATCAATCCAGCAGTCCTAGAGCCTGAGAGGGAGCACCTGGATG
  AGAACAGCCCACTGGGGGACTTGCTGCGAGGGGTCTTAGATGTGCCAGCTTGTCAGATCGCCATCCGTCC
  TGAGGGCAAAAACAACCGGCTCTTCGTCTTCTCCATCAGCATGCCATCAGTGGCTCAGTGGTCCCTGGAT
  GTTGCAGCTGACTCACAGGAGGAGTTACAGGACTGGGTGAAAAAGATCCGTGAAGTTGCCCAGACTGCAG
  ATGCCAGGCTCACTGAGGGAAAGATGATGGAGAGGAGGAAGAAGATCGCCTTGGAGCTCTCCGAGCTTGT
  GGTCTACTGCCGGCCCGTTCCCTTTGATGAAGAGAAGATTGGCACAGAACGTGCTTGTTACCGGGACATG
  TCCTCCTTTCCGGAAACCAAGGCTGAGAAGTATGTGAACAAGGCCAAAGGCAAGAAGTTCCTCCAGTACA
  ACCGGCTGCAGCTCTCGCGCATCTACCCTAAGGGCCAGAGGCTAGACTCCTCCAATTATGACCCTCTGCC
  CATGTGGATCTGCGGTAGCCAGCTTGTAGCACTCAATTTCCAGACCCCAGACAAGCCTATGCAGATGAAC
  CAGGCCCTCTTCATGGCTGGTGGGCATTGTGGCTATGTGCTGCAGCCAAGCACCATGAGAGACGAAGCCT
  TTGACCCCTTTGATAAGAGCAGTCTCCGAGGTCTGGAACCCTGTGTCATTTGCATTGAGGTGCTGGGGGC
  CAGGCATCTGCCGAAGAATGGCCGGGGTATTGTGTGTCCTTTTGTGGAGATTGAGGTGGCTGGGGCTGAG
  TACGACAGCACCAAGCAAAAGACGGAGTTTGTAGTGGACAACGGACTGAACCCTGTGTGGCCTGCTAAGC
  CCTTCCACTTCCAGATCAGTAACCCAGAGTTTGCCTTTCTGCGCTTTGTGGTGTATGAGGAAGACATGTT
  TAGTGACCAGAACTTCTTGGCTCAGGCTACTTTCCCAGTAAAAGGCCTGAAGACAGGATATAGAGCAGTG
  CCTTTGAAGAACAACTACAGTGAAGACCTGGAGTTGGCCTCCCTGCTCATCAAGATTGACATTTTCCCTG
  CTAAGGAGAACGGTGACCTCAGTCCTTTCAGTGGCATATCCCTAAGGGAACGGGCCTCAGATGCCTCCAG
  CCAGCTGTTCCATGTCCGGGCCCGGGAAGGGTCCTTTGAAGCCAGATACCAGCAGCCATTTGAAGATTTC
  CGCATCTCGCAGGAGCATCTAGCAGACCATTTTGACAGTCGGGAACGAAGTACTTCAGATGGTCCTTCCT
  CAGCCACTAACCTTATTGAGGATCCCTTACATGACAAGCTGTGGAAGTGCTCTCTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021280
• Insert Size: 3909 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021280.3, NP_067255.2
• RefSeq Size: 4435 bp
• RefSeq ORF: 3909 bp
• Locus ID: 18803
• UniProt ID: Q62077
• Cytogenetics: 2 80.97 cM
• Gene Summary: Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.[UniProtKB/Swiss-Prot Function]