Zfp423 (NM_033327) Mouse Untagged Clone

CAT#: MC224172

Zfp423 (untagged) - Mouse zinc finger protein 423 (Zfp423), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zfp423
• Synonyms: ataxia1; Ebfaz; mKIAA0760; nur12; Roaz; Zfp104; Znf423
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224172 representing NM_033327
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGGCGGAAGCAGGCGAAGCCGCGATCGGTGAAAGTTGAAGAGGGGGAGGCCTCGGACTTCTCGC
  TGGCCTGGGATTCCTCTGTGGCAGCAGCAGGAGGCCTGGAAGGAGAGCCAGAGTGTGATCGGAAAACCAG
  CCGTGCGCTGGAAGACAGGAACAGCGTGACAAGTCAAGAGGAGAGAAATGAGGACGATGAAGACGTGGAA
  GATGAGTCAATTTACACCTGCGATCACTGTCAGCAGGACTTCGAGTCTCTGGCAGACCTGACGGACCACC
  GGGCCCACCGCTGTCCTGGAGATGGTGATGACGACCCACAGCTCTCCTGGGTGGCTTCATCTCCCTCCAG
  CAAGGATGTTGCGTCACCTACGCAGATGATAGGAGATGGTTGTGACCTCGGCCTCGGCGAGGAGGAAGGC
  GGCACCGGCCTGCCCTACCCTTGCCAGTTCTGCGACAAGTCCTTCATCCGCCTGAGCTACTTGAAGAGGC
  ATGAACAGATCCACAGCGACAAGCTGCCGTTCAAGTGCACCTTCTGCAGCCGCCTCTTCAAACACAAGAG
  GAGCCGAGACCGGCACATCAAGCTGCACACGGGCGACAAGAAGTACCACTGTCACGAGTGCGAGGCAGCT
  TTCTCCCGCAGCGACCACCTCAAGATCCACCTGAAGACCCACAGCTCCAGCAAGCCGTTCAAGTGCAGCG
  TGTGCAAGCGCGGCTTCTCCTCCACCAGCTCCCTGCAGAGCCACATGCAGGCCCACAAGAAGAACAAGGA
  ACACCTGGCCAAGTCGGAGAAGGAAGCCAAGAAGGACGACTTCATGTGCGACTACTGCGAGGATACCTTT
  AGCCAGACAGAGGAGCTGGAGAAGCACGTGCTCACCCTCCACCCGCAGCTCTCAGAGAAGGCGGATCTAC
  AGTGCATCCACTGCCCAGAGGTCTTTGTTGATGAGAGCACGCTGCTTGCCCACATCCACCAAGCCCACGC
  CAACCAGAAACACAAGTGCCCCATGTGCCCTGAGCAGTTCTCCTCTGTGGAGGGTGTGTACTGCCACCTG
  GACAGCCACCGGCAGCCCGACTCCAGTAACCACAGCGTCAGCCCTGACCCTGTGCTGGGCAGTGTGGCTT
  CCATGAGCAGTGCTACACCTGACTCCAGCGCCTCTGTGGAGCGCGGGTCCACGCCAGACTCCACCTTGAA
  GCCACTGAGGGGGCAGAAGAAGATGCGGGATGATGGGCAGAGCTGGCCCAAGGTCGTCTACAGCTGCCCC
  TATTGTTCTAAGCGGGACTTTACCAGCCTGGCTGTGCTAGAGATTCATCTGAAGACCATTCACGCGGATA
  AACCTCAGCAGAGTCACACGTGTCAGATCTGCCTTGACTCCATGCCCACGCTCTACAACCTCAATGAGCA
  TGTGCGCAAGCTGCACAAGAGCCACGCCTACCCCGTCATGCAGTTTGGCAACATCTCTGCCTTCCACTGC
  AACTACTGCCCCGAGATGTTTGCTGACATCAACAGCCTGCAGGAGCACATTCGAGTCTCGCACTGTGGCC
  CCAATGCCAACCCCCCTGATGGGAATAATGCTTTCTTCTGCAACCAGTGCTCCATGGGCTTCCTCACCGA
  GTCCTCGCTCACAGAGCACATCCAACAGGCGCATTGCAGTGTGGGGAGCACCAAGCTGGAGTCTCCTGTT
  GTTCAGCCCACACAGTCCTTCATGGAGGTCTACTCCTGCCCTTACTGTACCAACTCCCCTATCTTTGGCT
  CCATCCTGAAGCTCACTAAACACATTAAAGAGAACCACAAGAACATCCCGCTGGCACACAGCAAGAAGTC
  CAAGGCTGAGCAGAGCCCAGTCTCCTCCGACGTCGAGGTGTCTTCCCCGAAACGACAGCGCCTCTCGGGG
  AGCGCCAACTCCATCTCTAATGGCGAGTACCCCTGCAATCAGTGCGACCTCAAGTTCTCCAACTTCGAGA
  GCTTCCAGACCCACCTGAAGCTGCACCTGGAGCTGCTGCTTCGGAAACAGGCCTGCCCCCAGTGCAAAGA
  GGACTTCGACTCTCAGGAGTCCCTCCTGCAGCATCTGACGGTGCATTACATGACTACATCCACCCACTAC
  GTGTGCGAGAGCTGTGACAAGCAGTTCTCCTCGGTGGATGACCTACAGAAGCACCTGCTGGACATGCACA
  CCTTTGTGCTATACCACTGCACGCTGTGTCAGGAGGTCTTCGACTCCAAGGTGTCCATCCAGGTGCACCT
  GGCCGTGAAGCACAGCAACGAGAAGAAGATGTACCGCTGCACAGCCTGCAACTGGGACTTCCGCAAGGAG
  GCTGACCTGCAGGTGCACGTCAAACACAGCCACCTCGGCAACCCGGCCAAGGCCCACAAGTGCATCTTCT
  GCGGCGAGACCTTCAGCACCGAGGTGGAGCTGCAGTGCCACATCACCACACACAGCAAGAAGTACAACTG
  CAGGTTCTGCAGCAAGGCCTTCCATGCCGTCATCCTGCTGGAGAAGCACCTGCGGGAGAAGCACTGCGTG
  TTTGACGCTGCCGCGGAGAATGGCACGGCCAACGGGGTGCCCCCCACCTCCACCAAGAAGGCAGAGCCCG
  CCGACCTGCAGGGCATGCTGCTCAAGAATCCTGAGGCGCCGAACAGCCACGAGGCCAGTGAGGATGACGT
  GGACGCATCAGAGCCCATGTATGGCTGTGACATCTGCGGTGCAGCCTACACCATGGAGGTGTTGCTGCAG
  AACCACCGCCTCCGGGATCACAACATCCGGCCCGGCGAGGACGACGGCTCACGCAAGAAGGCAGAATTCA
  TAAAGGGCAGCCATAAGTGTAACGTGTGCTCTCGGACTTTCTTCTCGGAGAACGGGCTCCGGGAACACCT
  GCAGACGCACCGGGGCCCTGCCAAGCACTACATGTGTCCCATCTGTGGCGAGCGCTTCCCCTCGCTGCTG
  ACACTCACCGAGCACAAGGTGACCCACAGCAAGAGTCTGGACACGGGCACCTGTCGCATCTGCAAAATGC
  CACTGCAGAGTGAGGAGGAGTTCATTGAGCACTGCCAGATGCACCCCGACTTACGGAACTCCCTCACCGG
  CTTCCGCTGTGTGGTCTGTATGCAGACTGTCACCTCGACGCTGGAACTCAAGATCCATGGCACCTTTCAC
  ATGCAGAAGCTGGCTGGCAGCTCAGCTGCCTCCTCCCCCAATGGCCAGGGACTGCAGAAGCTCTACAAAT
  GCGCCCTGTGCCTCAAAGAGTTCCGTAGCAAGCAGGACCTGGTCAGGCTTGATGTCAATGGGCTGCCCTA
  TGGCCTGTGTGCCGGCTGCATGGCCCGGAGTGCCAATGGACAGGTGGGTGGCCTGGCCCCACCCGAACCT
  GCTGACCGGCCCTGTGCCGGCCTCCGCTGCCCTGAATGTAACGTGAAGTTCGAGAGTGCTGAGGACCTGG
  AGAGCCACATGCAGGTGGACCACCGTGATCTTACACCAGAGACCAGTGGGCCCCGGAAAGGTGCCCAGAC
  GTCACCAGTGCCCAGGAAGAAGACGTACCAGTGTATCAAGTGCCAGATGACGTTCGAGAACGAGAGAGAG
  ATTCAGATCCACGTCGCCAACCACATGATCGAGGAAGGCATTAACCATGAGTGTAAACTGTGCAACCAGA
  TGTTCGACTCCCCAGCCAAGCTCCTCTGCCACCTCATTGAGCACAGCTTCGAGGGCATGGGCGGTACCTT
  CAAGTGCCCCGTCTGCTTCACAGTCTTCGTCCAGGCCAACAAGCTGCAACAGCATATCTTTGCCGTGCAC
  GGGCAGGAGGACAAAATCTACGACTGCTCGCAGTGCCCACAGAAGTTTTTCTTCCAGACAGAGTTGCAGA
  ACCACACGATGAGCCAGCACGCACAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_033327
• Insert Size: 3879 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033327.2, NP_201584.2
• RefSeq Size: 4916 bp
• RefSeq ORF: 3879 bp
• Locus ID: 94187
• UniProt ID: Q80TS5
• Cytogenetics: 8 C3
• Gene Summary: Transcription factor that can both act as an activator or a repressor depending on the context. Plays a central role in BMP signaling and olfactory neurogenesis. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved in terminal olfactory receptor neurons differentiation; this interaction preventing EBF1 to bind DNA and activate olfactory-specific genes. Involved in olfactory neurogenesis by participating in a developmental switch that regulates the transition from differentiation to maturation in olfactory receptor neurons. Controls proliferation and differentiation of neural precursors in cerebellar vermis formation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).