Astn1 (NM_007495) Mouse Untagged Clone

CAT#: MC224157

Astn1 (untagged) - Mouse astrotactin 1 (Astn1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Astn1
• Synonyms: Astn; GC14; mKIAA0289
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224157 representing NM_007495
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTAGCCGGGCTCTGCGCCCTGTTCGCCTGCTGCTGGGGGCCGGCTGCGGTGCTGGCCACAGCTG
  CCGGCGACGTGGATCCATCCAAGGAGCTGGAGTGCAAGCTCAAGAGCATCACTGTGTCGGCACTGCCCTT
  CCTGCGCGAGAACGACCTGAGCATCATGCACAGTCCCTCGGCCTCCGAACCCAAGCTTCTCTTCTCCGTG
  CGCAATGACTTCCCGGGAGAAATGGTCGTGGTGGACGACCTAGAGAACACGGAGCTACCCTATTTCGTGC
  TGGAGATCTCAGGGAATACAGAAGACATCCCCCTGGTGCGCTGGAGGCAGCAATGGCTGGAGAATGGCAC
  TTTACTCTTTCATATTCACCACCAAGATGGTGCTCCAAGTCTCCCTGGACAAGACCCAACTGAAGAACCC
  CAACATGAATCAGCAGAGGAAGAGCTGAGGATCCTGCACATCTCAGTCATGGGTGGCATGATCGCTCTGT
  TGCTATCCATCTTGTGCCTGGTGATGATCCTGTACACTCGCAGGCGCTGGTGCAAACGTCGCCGGGTTCC
  CCAGCCCCAGAAGAGTGCCAGTGCAGAGGCAGCAAATGAGATTCACTACATCCCATCTGTGCTGATTGGA
  GGCCATGGGCGGGAAAGCTTGCGCAATGCCCGTGTGCAGGGCCACAACTCCAGTGGCACTCTGAGCATTC
  GGGAGACACCCATCCTGGATGGCTATGAGTATGACATCACAGACCTGCGTCACCACCTGCAAAGGGAGTG
  TATGAATGGAGGGGAGGACTTTGCCAGTCAGGTCACACGCACTTTGGACTCCCTACAGGGATGCAATGAG
  AAGTCTGGGATGGATCTAACACCAGGGAGTGACAATGCCAAGCTTTCCCTGATGAACAAGTATAAAGACA
  ATATCATAGCCACAAGCCCCGTGGATTCTAACCACCAGCAAGCCACTCTGCTCTCCCACACCTCCAGCAG
  CCAGAGAAAGAGGATCAACAATAAAGCCAGAGCTGGTTCTGCCTTCTTGAACCCTGAAGGGGACTCCAGC
  ACAGAGGCCGAAAATGACCCACAGCTGACCTTCTACACAGACCCCTCTCGGAGCCGGAGGCGCAGTCGAG
  TGGGCTCTCCCCGAAGTCCTGTGAATAAGACCACCTTGACCCTGATCAGTGTCACCAGCTGCGTGATTGG
  CCTCGTGTGCTCTTCTCATGTCAGCTGCCCTCTTGTTGTCAAGATCACCCTACACGTCCCTGAGCACTTG
  ATCGCTGATGGGAGCCGCTTCATCCTGCTGGAGGGGAGCCAGCTGGATGCCAGTGACTGGCTGAACCCTG
  CCCAAGTGGTTCTCTTCTCTCAGCAGAACTCCAGCGGGCCCTGGGCCATGGACCTCTGTGCCCGGCGGCT
  CCTGGACCCTTGTGAACACCAATGTGACCCCGAAACTGGGGAATGCCTGTGCTATGAAGGCTACATGAAG
  GATCCTGTCCACAAGCACCTTTGCATTCGGAATGAATGGGGGACCAACCAGGGGCCTTGGCCTTACACGA
  TATTTCAGCGAGGCTTTGACCTGGTTTTGGGAGAGCAACCCTCTGATAAGATATTCAGATTCACCTATAC
  CCTCGGGGAAGGCATGTGGTTACCCCTCAGTAAGAGCTTTGTGATTCCACCAGCCGAACTAGCCATCAAT
  CCATCAGCAAAGTGTAAGACGGATATGACTGTGATGGAAGATGCTGTAGAGGTCAGAGAGGAGCTGATGA
  CATCATCATCCTTTGACAGCCTGGAGGTCCTATTAGACTCCTTTGGGCCAGTGCGTGATTGCAGCAAAGA
  TAACGGAGGCTGCAGTAAGAATTTTCGCTGCATTTCAGATCGCAAGTTGGACTCTACTGGTTGTGTGTGT
  CCTTCAGGACTCAGCCCTATGAAGGACAGCTCAGGCTGCTATGATCGCCATATCGGAGTGGACTGCTCAG
  ATGGTTTCAACGGCGGCTGCGAACAGCTGTGTCTCCAGCAGATGGCGCCGTTCCCAGAGGACCCCACCTT
  GTACAACATCCTTATGTTCTGCGGGTGTATCGAAGACTACAAGCTTGGTGTGGATGGACGCTCTTGCCAA
  CTTGTCACTGAGACCTGCCCAGAGGGAGGTGACTGTGGGGAAAGCAGAGAGGTTCCCATGAACCAGACTC
  TCTTTGGAGAAATGTTCTTTGGCTACAACAACCAGTCCAAGGAAGTAGCCACTGGACAGGTGCTAAAAGG
  AACATTCAGACAGAACAACTTTGCTCGTGGTTTAGACCAGCAACTGCCGGATGGTCTTGTGGTTGCCTCT
  GTCCCACTGGAGAATCAGTGCCTAGAGGAAATCTCAGAGCCCACCCCAGACCCTGACTTCTTGACTGGGA
  TGGTGAACTTCAGTGAAGTGTCTGGATACCCGGTGCTGCAGCACTGGAAGGTTCGGTCTGTGATGTACCA
  CATCAAACTCAACCAAGCAGCCATCTCGCAGGCCTTCAGCAATGCTCTTCACTCCTTGGATGGGGCTACA
  TCTCGTGCAGATTTTGTGGCTTTGTTGGATCAGTTTGGAAACCATTACATCCAGGAAGCTGTCTACGGCT
  TTGAGGAATCCTGTTCTATATGGTACCCAAACAAGCAAGTCCAGCGGAGACTCTGGCTGGAATATGAAGA
  CATCAGTAAAGGCAACTCTCCATCTGATGAGTCGGAGGAGCGGGAAAGGGATCCCAAGGTGCTGACATTC
  CCAGAATACATCGCTAGCCTGTCAGACTCTGGCACCAAGCGAATGGCAGCTGGAGTCCGGATGGAGTGCC
  AGAGCAAGGGACGGTGTCCCTCATCCTGTCCTTTATGTCATGTGACATCCAGCCCTGAAACCCCTGCTGA
  GCCAGTTCTACTGGAAGTGACCAGAGCATCCCCCATCTATGAACTGGTGACCAATAACCAGACCCAGAGG
  CTCTTACAGGAAGCCACCATGAGCTCTCTCTGGTGTTCAGGGACCGGAGATGTCATTGAGGACTGGTGCC
  GATGCGACTCGACTGCTTTTGGAGCAGATGGACTTCCTACCTGTGCACCCCTCCCACAGCCTGTGCTGAG
  ACTTTCCACAGTACACGAGCCCAGCAGTAACCTCGTGGTCCTGGAGTGGGAACATTCAGAGCCGCCAATT
  GGGGTGCAGATTGTAGACTACCTGATCCGGCAAGAGAAAGTCACTGACCGGATGGACCACTCCAAAGTAG
  AGACGGAAACAGTGCTGAGCTTTGTAGACGACATCATCTCTGGAGCAAAGGCTCCATGTGCCATGCCGTC
  TCAGGTGCCAGACAAACAGCTCACCACGATTTCTCTCATCATCCGATGCCTGGAACCTGACACCATTTAC
  ATGTTCACCCTCTGGGGAGTAGATAACACAGGGCGACGTTCCAGGCCAAGTGATGTGATTGTGAAGACCC
  CATGTCCTGTGGTGGATGATGTCAAAGCCCAAGAAATAGCAGACAAGATCTACAATCTCTTCAATGGCTA
  CACCAGTGGAAAGGAGCAACAGACTGCCTACAACACCCTTCTGGATCTGGGTTCTCCCACTTTGCACCGA
  GTCCTCTACCACTATAACCAGCACTATGAGAGTTTTGGGGAATTCACCTGGCGGTGTGAAGATGAGTTAG
  GACCCAGGAAAGCAGGCCTCATCCTTTCCCAGCTTGGAGATCTGAGCAGCTGGTGCAATGGACTCCTTCA
  GGAGCCCAAGATAAGCTTGAGGCGTGGTTCACTCAAGTACCTGGGCTGCCGCTACAGTGAGATCAAACCC
  TATGGACTGGACTGGTCAGAGCTCAGTCGGGACCTCAGGAAGACATGTGAAGAACAGACCCTGAGTGTCC
  CCTACAATGACTACGGGGACAGCAAAGACATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007495
• Insert Size: 3885 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007495.4, NP_031521.2
• RefSeq Size: 7217 bp
• RefSeq ORF: 3885 bp
• Locus ID: 11899
• UniProt ID: Q61137
• Cytogenetics: 1 H1
• Gene Summary: Neuronal adhesion molecule that is required for normal migration of young postmitotic neuroblasts along glial fibers, especially in the cerebellum. Required for normal rate of migration of granule cells during brain development and for normal cerebellum development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.