Ehmt2 (NM_145830) Mouse Untagged Clone

CAT#: MC224113

Ehmt2 (untagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant long, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ehmt2
• Synonyms: Bat8; D17Ertd710e; G9a; KMT1C; NG36
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224113 representing NM_145830
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGGTCTGCCGAGAGGGAGGGGGCTGATGCGGGCCCGGGGGCGGGGGCGTGCGGCCCCCACGGGCG
  GCCGCGGCCGCGGTCGGGGGGGCGCCCACCGAGGGCGAGGTAGGCCCCGAAGCCTGCTCTCGCTGCCCAG
  GGCCCAGGCGTCTTGGGCCCCCCAGCTGCCTGCCGGGCTGACCGGCCCCCCGGTTCCTTGTCTCCCCTCC
  CAGGGGGAGGCCCCCGCTGAGATGGGGGCGCTGCTGCTGGAGAAGGAGCCCCGAGGAGCCGCCGAGAGAG
  TTCATAGCTCTTTGGGGGACACCCCTCAGAGTGAGGAGACCCTTCCCAAGGCCAACCCCGACTCCTTGGA
  GCCTGCCGGCCCCTCCTCTCCGGCCTCTGTCACTGTCACCGTCGGCGATGAGGGGGCTGACACCCCTGTC
  GGGGCCGCATCACTCATCGGGGACGAACCCGAGAGCCTGGAGGGAGATGGGGGTCGCATCGTGCTGGGCC
  ATGCCACAAAGTCGTTCCCCTCTTCCCCCAGCAAGGGGGGTGCCTGTCCCAGTCGGGCCAAAATGTCAAT
  GACAGGGGCAGGAAAGTCGCCCCCCTCGGTCCAGAGTTTGGCCATGAGGCTGTTGAGCATGCCCGGGGCC
  CAGGGAGCTGCAACTGCTGGGCCTGAACCCTCTCCGGCAACAACTGCCGCCCAGGAGGGGCAGCCCAAAG
  TGCACCGAGCCCGGAAAACCATGTCCAAACCTAGCAACGGACAGCCTCCAATCCCTGAGAAGCGGCCCCC
  TGAAGTCCAGCATTTCCGCATGAGTGATGACATGCATCTGGGGAAGGTGACTTCAGATGTGGCCAAAAGG
  AGGAAGCTGAACTCTGGTAGCCTGTCCGAGGACTTGGGCTCTGCCGGGGGCTCAGGAGATATAATCCTGG
  AGAAGGGAGAGCCCAGGCCCCTGGAGGAGTGGGAGACGGTGGTGGGCGATGACTTCAGCCTGTACTATGA
  TGCGTACTCTGTGGATGAGCGGGTGGACTCTGACAGCAAGTCTGAAGTCGAAGCTCTAGCTGAACAGTTG
  AGTGAGGAGGAGGAGGAGGAAGAGGAGGAAGAAGAAGAAGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGG
  AAGAAGAGGACGAGGAGTCGGGCAATCAGTCAGACAGGAGCGGTTCTAGTGGCCGGCGCAAGGCCAAGAA
  GAAATGGCGGAAAGACAGCCCGTGGGTGAAGCCATCTAGAAAACGGCGGAAACGAGAGCCTCCGAGGGCC
  AAGGAGCCAAGAGGAGTGAATGGTGTGGGTTCCTCAGGGCCCAGTGAGTACATGGAGGTTCCTCTGGGGT
  CCCTGGAGCTGCCCAGCGAGGGGACCCTCTCCCCCAACCACGCTGGGGTCTCCAATGACACGTCTTCACT
  GGAGACAGAACGCGGGTTTGAGGAGCTGCCCCTCTGCAGCTGCCGCATGGAGGCTCCCAAGATTGACCGC
  ATCAGCGAGAGAGCAGGGCACAAGTGCATGGCCACAGAGAGTGTGGATGGAGAGCTCCTGGGCTGCAATG
  CTGCCATCCTTAAGCGGGAGACCATGCGGCCGTCTAGCCGCGTGGCGCTGATGGTGCTCTGTGAGGCCCA
  TCGAGCCCGCATGGTCAAGCACCATTGCTGCCCGGGCTGCGGCTACTTCTGCACAGCGGGCACCTTCCTG
  GAATGCCACCCCGACTTTCGTGTAGCTCACCGCTTCCATAAGGCCTGCGTATCCCAGCTCAATGGGATGG
  TCTTCTGTCCCCACTGTGGAGAGGATGCCTCAGAGGCCCAGGAGGTGACCATTCCTCGGGGCGATGGGGG
  AACACCCCCAATTGGCACCGCAGCTCCTGCTCTGCCACCCCTGGCACATGATGCCCCAGGGCGAGCGGAT
  ACCTCCCAGCCTAGCGCCCGAATGCGAGGGCATGGAGAGCCGCGGCGCCCGCCCTGTGATCCCCTGGCTG
  ACACCATCGACAGCTCAGGGCCTTCACTGACTCTGCCTAATGGGGGCTGCCTCTCCGCTGTGGGTCTGCC
  CCCAGGGCCGGGCAGGGAAGCCCTGGAAAAAGCCTTGGTCATCCAGGAGTCTGAGAGGCGGAAGAAGCTG
  CGATTCCACCCACGGCAGCTGTACCTGTCGGTGAAGCAGGGGGAGCTGCAGAAGGTGATCCTTATGCTGT
  TAGACAACCTGGACCCCAACTTCCAGAGCGACCAGCAGAGCAAGCGCACGCCCCTGCACGCGGCCGCCCA
  GAAGGGGTCGGTAGAGATCTGTCATGTGCTGCTGCAGGCAGGAGCCAACATCAATGCCGTAGATAAGCAA
  CAACGCACGCCACTAATGGAGGCCGTGGTGAACAACCACCTGGAGGTGGCACGCTACATGGTGCAGTTAG
  GTGGCTGTGTCTACAGCAAGGAAGAGGATGGCTCCACCTGTCTACATCATGCAGCCAAAATTGGGAACTT
  GGAAATGGTCAGCCTGCTACTGAGCACAGGACAGGTGGACGTCAATGCCCAGGACAGTGGGGGCTGGACG
  CCCATCATCTGGGCAGCCGAGCACAAGCACATCGATGTGATTCGTATGCTGCTGACCCGGGGTGCCGATG
  TCACCCTGACTGACAATGAGGAAAACATCTGCCTGCACTGGGCCTCCTTCACGGGTAGTGCCGCCATCGC
  TGAGGTCCTTCTGAATGCCCAGTGTGATCTCCATGCTGTCAACTACCATGGGGACACGCCCCTGCACATA
  GCCGCCAGGGAGAGCTACCATGACTGTGTTCTGTTGTTCCTGTCTCGTGGAGCCAACCCTGAGCTTCGGA
  ACAAAGAAGGAGACACGGCATGGGATCTGACCCCAGAGCGCTCTGATGTGTGGTTTGCACTGCAGCTCAA
  TCGAAAGCTTAGGCTTGGGGTAGGGAACCGGGCTGTCCGCACCGAGAAGATCATCTGCCGGGACGTAGCC
  CGAGGCTATGAGAATGTACCCATCCCCTGTGTCAATGGTGTGGATGGGGAGCCGTGCCCGGAGGACTACA
  AGTACATCTCTGAGAACTGCGAGACATCGACCATGAACATCGACCGCAACATCACCCATCTGCAGCACTG
  CACGTGTGTGGATGACTGCTCCAGCTCCAATTGCCTATGTGGTCAGCTCAGTATCCGATGCTGGTATGAC
  AAGGACGGGCGGCTGCTCCAGGAGTTTAACAAGATCGAGCCCCCCCTGATCTTTGAGTGTAACCAGGCAT
  GCTCCTGCTGGAGAAGCTGCAAGAACCGCGTGGTGCAGAGCGGCATCAAGGTACGGCTGCAGCTCTACCG
  GACTGCCAAGATGGGCTGGGGGGTCCGAGCCTTGCAGACCATCCCCCAGGGCACGTTCATCTGCGAGTAT
  GTAGGAGAGCTGATCTCTGATGCCGAGGCTGATGTGAGAGAGGATGATTCTTACCTCTTCGATTTAGATA
  ACAAGGATGGCGAGGTTTACTGCATTGATGCCCGTTACTATGGCAACATCAGCCGATTCATTAACCACCT
  GTGTGACCCCAACATCATCCCTGTCCGGGTTTTCATGCTGCACCAAGATCTACGGTTCCCACGCATTGCC
  TTCTTCAGCTCCAGGGACATCCGGACTGGGGAGGAGCTGGGCTTTGACTACGGTGACCGATTCTGGGACA
  TCAAGAGCAAGTATTTCACCTGCCAGTGTGGCTCTGAGAAGTGCAAGCATTCAGCGGAGGCCATCGCCCT
  GGAGCAGAGCCGCCTGGCCCGGCTGGACCCCCACCCGGAGCTGCTCCCTGACCTCAGCTCCCTGCCCCCC
  ATCAACACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145830
• Insert Size: 3792 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145830.2, NP_665829.1
• RefSeq Size: 4070 bp
• RefSeq ORF: 3792 bp
• Locus ID: 110147
• UniProt ID: Q9Z148
• Cytogenetics: 17 18.45 cM
• Gene Summary: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (a, also known as G9a long).