Arid4a (NM_001081195) Mouse Untagged Clone

CAT#: MC224098

Arid4a (untagged) - Mouse AT rich interactive domain 4A (RBP1-like) (Arid4a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arid4a
• Synonyms: A630009N03; A630067N03Rik; MmRBBP1; Rbbp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224098 representing NM_001081195
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCGGCAGATGAGCCTGCCTACCTGACAGTGGGAACTGATGTCAGTGCCAAGTACCGAGGGGCCT
  TCTGTGAGGCAAAGATCAAGACTGTGAAAAGGCTGGTGAAAGTCAAGGTACTTCTGAAGCAGGATAACAC
  AACACAGTTGGTGCAAGATGACCAAGTAAAGGGCCCTTTAAGAGTTGGAGCTATTGTAGAAACAAGGACA
  TCTGATGGATCTATCCAGGAAGCTATTATCAGCAAATTGACAGATGCTAGTTGGTATACTGTGGTGTTTG
  ATGACGGTGATGAGAGAACTCTGAGGCGGACCTCACTTTGCCTGAAAGGGGAGAGACATTTTGCCGAGAG
  TGAGACTCTGGATCAGCTTCCTCTAACAAACCCAGAGCATTTTGGGACGCCAGTCATTGCAAAGAAGACC
  AACAGAGGGAGGAGGTCCTCACTGCCTATCACTGAAGACGAAAAGGAAGAAGAAAGCAGCGAAGAGGAAG
  ATGAAGATAAGAGGCGTCTCAATGACGAATTATTAGGAAAAGTTGTAAGTGTGGCCTCCACAGCAGAGAG
  CACTGGATGGTACCCTGCTTTGGTTGTCTCTCCCAGCTGCAATGATGACGTCACAGTGAAAAAAGATCAG
  TGTTTAGTTCGATCATTTATTGATTCTAAATTTTATTCTATAGCAAGAAAGGACATTAAGGAACTGGACA
  TTCTCACTCTACCAGAATCTGAGCTCTGTGCTAGGCCAGGGCTGCGAAGAGCAAGTGTCTTCCTAAAAGG
  TAGGATTGTTCCTGATAATTGGAAAATGGATATAAGTGAAATCCTTGAGTCCTCCAGCAGTGATGACGAA
  GAATGCCCAGCTGAGGAACATGAAGAGGAGAAGGAAAAAGAGGCCAAAAAGGAGGAAGAAGAGCTGCCTG
  AGGAAGAGCTTGATCCTGAGGAGAGGGACAATTTCCTCCAACAGCTTTATAAGTTTATGGAAGACAGAGG
  TACTCCGATCAACAAACCACCTGTTTTGGGCTATAAAGATCTCAATCTCTTCAAACTCTTTAGACTGGTC
  TATCATCAGGGGGGATGTGGCAATATTGACAGTGGTGCTGTATGGAAGCAAATTTATATGGACCTTGGCA
  TTCCAATTTTGAATTCAGCTGCTTCCTACAATGTAAAAACTGCTTATAGAAAATATCTCTATGGTTTTGA
  GGAGTACTGCCGTTCTGCAAATATTCAGTTCAGAACTATTCACCACCATGAACCAAAAGTAAAAGAGGAA
  AAAAAAGACTTTGAAGACTCAATGGACGAAGCTCTAAAAGAGGCCCCGGAAATGCCTTTGCTGGACGTGA
  AGAGTGAGCCCGAGGAGAATACGGACTCAAACAGTGAAAGCGACAGAGAAGACACAGAGCTAAAGTCGCC
  AAGAGGGAGAAGGAAAATTGTTCGAGATGCAAACTGTATCAAGAAGGAAATAGAGGAAGAGAAAATCGAA
  GACAAATTCCTCCGAGATGATTTAGAAAATAAGGACGCAGGTGATGACGATGACGATGGCGACCCAGCAG
  CGAAGAGAGAGCATGAGCTGCTGTTCGGGAGGAAGAGCACACCAAAGAACAAAGAGAAGAAGATTAAAAA
  GCCAGAGGACTCTGAGAGAGACTCAGATGAAGAGGAAGAGAAGAGCCAAGAGAGGGAAGAGACTGAGAGC
  CGGTGTGACTCGGAAGGGGAGGACGAGGAGGACGACACAGAGCCCTGCCTGACGGGAACCAAAGTGAAGG
  TCAAGTACGGACGAGGAAAAACTCAGAAAATTTATGAAGCCAGCATCAAAAGCACGGAGATGGACGACGG
  GGAGATTCTGTACCTAGTGCACTACTACGGATGGAACGTCAGATATGATGAATGGGTAAAAGCTGACAGG
  ATAATCTGGCCCTTGGACAAAGGTGGACCAAAGAAAAAACAAAAGAAGAAAGTTAAGAATAAAGAAGATA
  GTGAAAAGGACGAAAAGAGGGATGAAGAGAGGCAGAAGTCCAAACGGGGAAGACCACCTTTAAAATCTAC
  TTTTTCGCCAAACATGCCATACAGTTTATCTAAGACGTCAAACAGTGAAGGAAAATCAGACTCCTGTTCA
  TCTGATAGTGAGGCAGACGACCAGCTGGAGAAGAGCTCAGGGGGTGAAGATCTTTCTCCTGATGTCAAAG
  AAGAATTGGAAAAAAATGAAAATGCACATGATGATAAACTAGATGAAGAAAACCCAAAGATTGTACATAT
  ATCAAAAGAAAACGACAGGACTCAGGCACAGCCCTCAGACACCCTGACGGTGGAGGCAGGGGACAGCGAT
  CAAATTGTGCATATTTTTGGAGACAAAGTGGACCAAGTAGAAGAATTTAAGAAACAGGTGGAAAAGTCTC
  CCAAAGGGAAGGGAAGGCGCAGCAAGACAAAAGACCTTTCGTTAGAACTTATAAAGATTTCACCTTTCGG
  CCAGGAGGAAGCAGGCAGCGAAGCTCATGGGGACGTTCATAGCCTGGAGTTTTCTTCACTAGAATGTAAA
  AACTTTTCTTCTACTGAAGATGACATCGACCCATATGAGAAAGAAAAAAAGTTGAAACGAAAAATCCTGG
  GACAGCAATCCCCAGAAAAAAAATTGAGACTTGATAATGGCATGGAAATGACAACTGGAGTGTCCCAAGA
  GAGGTCTGACGATGGCGCGGGAGCCGAGGGCATGAAAGGCGCACACGTGGAACAGCACTTTGAAACAGAA
  GGTGAAGGGATGCCATCGCTGACAGCCGAGCCCGACCAGGGCCTTCAAGAACTGACTAGTGAGAAATCGG
  ACAGTCCAGCCGAAGAAGAACCGGTGCACACGCCACTCAAAGAAGAGGAGGATGCGATGCCTCTGATCGG
  GCCTGAAACCTTGGTTTGCCATGAGGTAGACCTGGATGATTTAGATGAGAAGGATAAGACCAGTATTGAG
  GATGTGGTAGTAGAAGGCTCTGAGTCTAACTCTCTGGCTTCCGTACCACCTGCCTTACCTCCTGTAGCAC
  AGCATAACTTTTCCGTAGCTTCCCCACTCACTCTCAGCCAAGACGAGTCCCGAAGTATAAAGAGTGAGAG
  TGACATTACAATTGAAGTTGATAGCATCGCCGAAGAATCACAAGAAGGCCTCTGTGAGAGGGAATCGGCG
  AACGGGTTTGAAGCCAGTGTTGCCTCTGGTGCCTGTAGTATAATTGCACACGAGCGAGAGAGCAGAGAGA
  AGGGTCAGAAAAGGCCAAGCGATGGAAATAGTGGATTAATTGCAAAAAAGCAAAAGCGAACCCCAAAGCG
  GACAAGTGCTGCAGCCAAAACTGAAAAGAACGGAGCAGGACAGAGCAGTGACAGCGAGGACCTCCCCGCC
  ATGGACAGCTCCAGTAACTGCACCCCAGTGAAGCGCCTTACCCTGCCCAAGTCACAGAAGCTGCCACGGT
  CTCCTGCAAGAACCTCGCCTCACATCAAAGATGCAGAGAAGGAGAAGCACCGAGAGAAGCATCCGAACTC
  CTCCCCCAGGACATACAAGTGGAGCTTCCAGCTCAATGAATTAGATAATATGAACAGTACAGAGAGAATC
  TCTTTTCTCCAAGAAAAACTACAGGAAATCAGAAAATACTACATGTCTTTGAAGTCTGAAGTTGCAACCA
  TCGACAGAAGGAGGAAAAGATTGAAAAAGAAAGACAGAGAAGTGTCTCACGCAGGAGCGTCAATGTCCTC
  TGCTTCATCAGACACTGGAATGAGCCCTTCTTCATCGTCTCCCCCCCAAAATGTACTTGCTGTAGAGTGC
  AGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081195
• Insert Size: 3786 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081195.1, NP_001074664.1
• RefSeq Size: 5780 bp
• RefSeq ORF: 3786 bp
• Locus ID: 238247
• UniProt ID: F8VPQ2
• Cytogenetics: 12 C2
• Gene Summary: DNA-binding protein which modulates activity of several transcription factors including RB1 (retinoblastoma-associated protein) and AR (androgen receptor) (PubMed:17043311, PubMed:23487765). May function as part of an mSin3A repressor complex (By similarity). Has no intrinsic transcriptional activity (PubMed:23487765). Plays a role in the regulation of epigenetic modifications at the PWS/AS imprinting center near the SNRPN promoter, where it might function as part of a complex with RB1 and ARID4B (PubMed:17043311). Involved in spermatogenesis, together with ARID4B, where it acts as a transcriptional coactivator for AR and enhances expression of genes required for sperm maturation (PubMed:23487765). Regulates expression of the tight junction protein CLDN3 in the testis, which is important for integrity of the blood-testis barrier (PubMed:23487765). Plays a role in myeloid homeostasis where it regulates the histone methylation state of bone marrow cells and expression of various genes involved in hematopoiesis (PubMed:18728284). May function as a leukemia suppressor (PubMed:18728284).[UniProtKB/Swiss-Prot Function]