Stag1 (NM_009282) Mouse Untagged Clone

CAT#: MC224081

Stag1 (untagged) - Mouse stromal antigen 1 (Stag1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Stag1
• Synonyms: AU045003; SA-1; Scc3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224081 representing NM_009282
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTACTTCAGAGTTACCAGTGTTACAGGATTCAACTAATGAAACTACTGCCCACTCTGATGCTGGCA
  GCGAACTTGAAGAAACAGAGGTCAAAGGAAAAAGAAAAAGGGGTCGTCCTGGCCGGCCTCCATCAACAAA
  TAAGAAACCTCGAAAATCTCCAGGAGAAAAGAGCAGAATTGAAGCTGGAATTCGAGGAGCAGGTCGTGGA
  AGAGCCAATGGGCACCCCCAACAGAACGGCGACGGGGATCCTGTCACCTTATTTGAGGTGGTGAAGCTGG
  GGAAGAGTGCAATGCAGTCCGTGGTGGATGACTGGATTGAATTATATAAACAAGACAGGGACATCGCACT
  TCTGGATTTAATCAACTTTTTTATCCAGTGTTCAGGATGTCGAGGTACGGTCAGAATAGAGATGTTTCGA
  AATATGCAGAACGCAGAAATAATCAGAAAAATGACTGAAGAATTTGATGAGGACAGTGGTGACTACCCCC
  TTACAATGCCTGGTCCTCAGTGGAAAAAATTTCGTTCCAATTTTTGTGAATTTATTGGAGTCCTGATTCG
  ACAGTGTCAATATAGCATAATTTATGATGAATATATGATGGACACCGTAATTTCCCTTTTGACTGGTTTG
  TCAGACTCCCAAGTCAGAGCTTTTAGGCATACAAGTACCCTTGCTGCAATGAAGCTGATGACTGCTCTGG
  TGAATGTTGCTTTAAACCTCAGTATTCATCAAGATAATACACAGAGGCAATATGAAGCTGAGAGAAATAA
  AATGATTGGGAAGAGAGCCAATGAAAGACTGGAGTTACTACTTCAGAAACGTAAAGAGCTACAAGAAAAT
  CAAGATGAAATTGAAAATATGATGAACTCTATCTTTAAGGGTATATTTGTTCATCGATACCGTGATGCAA
  TTGCTGAAATTCGAGCCATCTGTATTGAAGAAATTGGAGTGTGGATGAAAATGTATAGCGATGCCTTCCT
  AAATGACAGTTACTTGAAATATGTAGGATGGACTCTTCACGATAGGCAAGGGGAAGTCAGACTGAAGTGT
  TTGAAAGCTCTACAAAGCCTGTATACCAATAGAGAATTATTCCCCAAATTGGAGCTATTTACAAATCGAT
  TCAAGGATCGCATTGTATCAATGACTCTTGACAAAGAATATGATGTTGCTGTGGAAGCAATCCGATTGGT
  TACTCTGATCCTTCATGGCAGTGAAGAAGCTCTTTCCAACGAAGACTGTGAAAATGTTTACCATTTGGTG
  TATTCAGCACATCGCCCTGTTGCTGTGGCAGCTGGAGAGTTCCTACACAAAAAGCTGTTCAGCAGACATG
  ACCCACAAGCAGAGGAAGCGTTAGCGAAGAGGAGAGGAAGGAACAGTCCAAACGGGAACCTCATTAGGAT
  GCTTGTTCTTTTCTTTCTGGAAAGTGAGTTACATGAACATGCAGCCTACTTGGTGGACAGCTTGTGGGAG
  AGCTCTCAAGAACTGTTGAAAGACTGGGAATGTATGACAGAGTTACTATTAGAAGAACCTGTTCAAGGAG
  AAGAAGCAATGTCTGACCGTCAAGAGAGTGCTCTTATAGAGCTAATGGTCTGTACAATTCGTCAGGCAGC
  TGAGGCACATCCTCCAGTGGGAAGGGGTACTGGCAAGAGAGTGCTAACAGCCAAAGAAAGGAAAACTCAA
  ATTGATGATAGGAACAAATTAACTGAACATTTTATCATTACACTTCCTATGTTACTATCAAAGTATTCTG
  CAGATGCTGAGAAGGTAGCAAACTTGCTGCAAATTCCACAGTATTTTGATCTAGAAATCTACAGCACAGG
  TCGGATGGAAAAGCACCTGGATGCTTTATTAAAACAAATTAAGTTTGTTGTAGAGAAACATGTAGAATCA
  GATGTCCTAGAAGCCTGCAGTAAAACCTACAGCATTTTATGCAGTGAAGAGTATACCATTCAGAATCGAG
  TTGATATTGCTCGGAGCCAACTGATTGATGAATTTGTAGATCGATTCAATCATTCTGTAGAAGACCTGTT
  GCAAGAGGGTGAAGAAGCTGACGATGATGATATCTACAATGTTCTTTCCACCTTAAAGCGTTTAACTTCT
  TTTCACAATGCTCATGATCTCACTAAATGGGATCTATTTGGTAATTGCTACAGACTATTGAAGACTGGAA
  TTGAACATGGAGCTATGCCAGAACAGATAGTCGTACAAGCACTGCAGTGTTCCCATTACTCAATTCTGTG
  GCAGTTGGTGAAAATTACTGACGGATCTCCTTCCAAAGAGGATTTGTTGGTATTGAGGAAAACAGTGAAG
  TCTTTTCTGGCTGTTTGCCAACAGTGCCTATCTAATGTTAATACTCCAGTTAAGGAACAGGCTTTCATGC
  TGCTCTGTGATCTTTTAATGATTTTTAGCCACCAATTAATGACAGGTGGCAGAGAGGGCCTTCAGCCTTT
  GGTGTTTAATCCAGATACTGGACTCCAGTCTGAACTCCTCAGTTTTGTGATGGATCATGTTTTCATTGAC
  CAAGATGAGGAAAACCAAAGCATGGAGGGTGATGAGGAAGATGAAGCTAATAAAATTGAAGCCTTACATA
  AAAGGAGGAACCTGCTTGCTGCCTTCAGCAAACTTATCATTTATGATATTGTTGACATGCACGCAGCTGC
  AGACATCTTTAAACACTACATGAAGTATTACAATGACTATGGTGATATTATTAAGGAAACACTGAGTAAG
  ACCAGGCAGATTGATAAAATTCAGTGTGCTAAGACTCTCATTCTCAGTCTGCAACAGTTGTTTAATGAAC
  TTGTTCAAGAACAAGGTCCTAATCTGGATAGGACATCTGCCCACGTCAGTGGGATTAAAGAACTGGCACG
  TCGTTTTGCCCTTACATTTGGATTGGACCAGATCAAGACCCGAGAAGCTGTTGCCACACTTCACAAGGAT
  GGAATAGAGTTTGCCTTTAAATACCAGAATCAGAAAGGACAAGAATACCCACCCCCTAATCTGGCTTTTC
  TAGAAGTACTAAGTGAATTTTCTTCTAAACTCCTTCGACAGGACAAGAAAACTGTTCATTCGTATCTTGA
  AAAATTCCTCACCGAGCAGATGATGGAAAGGAGGGAGGATGTGTGGCTTCCACTCATCTCGTATAGAAAC
  TCATTAGTCACTGGAGGTGAAGATGACAGGATGTCTGTGAACAGTGGAAGTAGCAGCAGTAAAACGTCCT
  CAGTAAGGAGTAAGAAAGGAAGACCCCCACTGCACAGAAAACGAGTAGAAGATGAAAGTCTGGATAACAC
  ATGGCTAAATAGGACTGACACGATGATTCAGACTCCTGGACCCTTGCCAACCCCACAGCTCACCTCCACG
  GTACTTAGAGAAAACAGCCGTCCCATGGGAGAGCAGATTCAGGAACCTGAGTCTGAGCATGGCTCTGAAC
  CAGACTTTTTACATAATCCCCAGATGCAGATCTCTTGGTTAGGCCAGCCGAAGTTAGAAGACTTGAATCG
  GAAGGACAGAACAGGGATGAACTACATGAAAGTAAGAGCTGGAGTCCGGCACGCCGTTCGGGGTCTAATG
  GAGGAAGATGCTGAGCCCATCTTTGAAGATGTGATGATGTCATCACGGAGCCAGTTAGAAGACATGAATG
  AAGAATTTGAAGACACCATGGTTATTGATTTGCCCCCATCAAGAAATCGCCGAGAGAGAGCTGAGCTAAG
  GCCAGACTTCTTTGACTCTGCAGCTATCATAGAAGATGATTCAGGATTTGGAATGCCTATGTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009282
• Insert Size: 3777 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009282.3, NP_033308.2
• RefSeq Size: 6010 bp
• RefSeq ORF: 3777 bp
• Locus ID: 20842
• UniProt ID: Q9D3E6
• Cytogenetics: 9 E3.3- E4
• Gene Summary: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).