Prg4 (NM_021400) Mouse Untagged Clone

CAT#: MC224000

Prg4 (untagged) - Mouse proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Prg4
• Synonyms: CACP; DOL54; JCAP; lubricin; MSF; SZP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224000 representing NM_021400
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTGGAAAATACTTCCCGTCTGCTTGTCGCTGCTGCTTCCTGTTGTCTTGATTCAACAGGTTTCAT
  CTCAAGATTTATCAAGCTGTGCAGGGAGATGTGGGGAAGGGTACTCTAGAGATGCCACCTGCAACTGTGA
  TTATAACTGTCAACACTACATGGAGTGCTGTCCTGATTTCAAGAGAGTCTGCACTACAGAGCTCTCCTGC
  AAAGGACGCTGCTTCGAGTCCTTCGCTCGAGGGAGGGAGTGTGACTGTGACTCCCAGTGTAAACAGTATG
  GCAAGTGCTGTGCAGATTATGACAGCTTCTGTGAAGAAGTGCATAATCCCACATCACCATCTCCAAAGAC
  TGCACCTCCGCCTCCAGGAGCATCTCACACCATCAAATCAACTACCAAACGCTCACCCAAATCACCAAAA
  AAGAAGACTAAGAAAGTTGTAGAATCTGAGGAAATAACAGAAGAACATTCTGTTTCTGAAAATCAAGAGT
  CCTCCTCCTCCTCCTCTTCTTCCTCTTCAACTATTCGGAAAATCAAGTCTTCCAAAAATTCAGCTAATAG
  AGAATTACAGAAGAACCCAAAAGTAAAAGATAACAAGAAAAACACTCCTAAAAAGAAACCCAATCCAGAA
  CCGCCGGCTGTGGATGAAGCTGGAAGCGGGCTGGATAATGGTGAGTTCAAGCTCACCCCACCTCCTCCTG
  ACCCTCCTACTACTCCACACAGCAAAGTCACCACATCTCCCAAGACTACAGCAGCAAAACCAGTAACTCC
  TAAACCCAGTCTTGCACCTAATTCTGAGACATCCAAAGAGGCATCTTTAGCATCCAATAAAGAGACAACA
  GTTGAAACGAAAGAGACTACTGCAACAAATAAACAATCTTCTGCCAGTAAAAAAAAGACGACTTCAGCTA
  AAGAGACACGGAGTGCAGAGAAAACATCTGATAAAGATGTTGAGCCCACATCTACAACTCCCAAGAACTC
  AGCTCCCACTACTACCAAGAAGCCTGTTACTACAACCAAGGAACCAGTTCCTACCACTCCCAAGGAGCCT
  GAACCCACAACTCCCAAGGAACCACCTCCAACCACCAAGAAGCCTGAACCCACCACCCCCAAGGAGCCTG
  AACCCACAACTCCCAAGGAGCCTGAACCCACCACCCCCAAGGAGCCTGAACCCACCACTCCCAAGGAGCC
  TGAACCCACAACTCCCAAGGAACCACCTCCAACCACCAAGAAGCCTGAACCCACCACCCCCAAGGAGCCT
  GAACCCACAACTCCCAAGGAGCCTGAACCCACCACCACCAAGGAGCCTGAACCCACCACCACCAAGGAGC
  CTGAATCCACCACCCGCAAGGAGCCTGAACCCACCACTCCCAAGGAGCCTGAACCCACAACTCCCAAGGA
  GCCTGAACCTACAACCCCCAAGGAGCCTGAACCCACAACTCCCAAGGAGCCTGAACCCACCACTCTCAAG
  GAGCCTGAACCCACCACGCCCAAGGAGCCTGAACCCACAACTCCCAAGGAGCCTGAACCTACAACCCCCA
  AGGAGCCTGAACCCACAACTCCCAAGGAGCCTGAACCCACAACTCCCAAGGAGCCTGAACCCACAACTCC
  CAAGGAGCCTGAACCCACAACTCCCAAGGAGCCTGAACCCACCACGCCCAAGGAGCCTGAACCCACAACC
  CCCAAGGAGCCTGAACCTACAACCCCCAAGGAGCCTGAACCCACAACTCCCAAGGAGCCTGAACCCACCA
  CTCTCAAGGAGCCTGAACCTACAACCCCCAAGGAGCCTGAACCCACCACTCCCAAGGAGCCTGAACCCAC
  CACTCTCAAGGAGCCTGAACCCACCACGCCCAAGGAGCCTGAACCCACCACTCTCAAGGAGCCTGAACCT
  ACAACCCCCAAGGAGCCTGAACCCACCACCACCAAGGAGCCTGAATCCACCACCCGCAAGGAGCCTGAAC
  CCACCACTCCCAAGGAGCCTGAACCCACAACTCCCAAGGAGCCTGAACCCACAACTCCCAAGGAGCCTGA
  ACCCACCACGCCCAAGGAGCCTGAACCCACAACTCCCAAGGAGCCTGAACCCACCACGCCCAAGGAGCCT
  GAACCCACAACCCCCAAGGAGCCTGAACCCACAACTCCCAAGAAACCTGAACCCACAACTCCCAAGGAGC
  CTGAACCTACAACCCCCAAGGAGCCTGAACCCACCACCCCCAAGGAGCCTGAACCCACCACGCCCAAGGA
  GCCTGAACCCACAACTCGCAAGGAGCCTGAACCTACAACTCCCAAGGAGCCTGAACCTACAACCCCCAAG
  GAGCCTGAACCCACAACTCCCAAGAAGCCTGAACCCACCACCACATCACCCAAAACCACTACTCTGAAAG
  CAACAACTCTTGCACCCAAAGTAACTGCTCCAGCAGAAGAGATTCAGAACAAACCTGAAGAAACAACTCC
  TGCATCAGAAGATTCTGATGATTCTAAAACAACTCTAAAACCACAGAAGCCAACCAAAGCACCCAAGCCT
  ACCAAAAAGCCAACCAAAGCACCCAAGAAGCCCACCTCTACCAAAAAGCCAAAGACACCAAAAACAAGAA
  AACCAAAAACTACACCATCTCCTCTAAAGACGACTTCAGCAACACCTGAACTGAATACCACCCCTCTAGA
  AGTCATGCTGCCAACCACCACCATCCCTAAACAAACTCCAAACCCTGAAACAGCTGAAGTAAATCCAGAT
  CATGAAGATGCAGATGGAGGTGAAGGAGAAAAACCTCTGATTCCCGGGCCCCCTGTGCTATTCCCCACAG
  CTATTCCAGGCACTGATCTTTTGGCCGGGAGACTCAATCAAGGCATTAACATCAATCCCATGCTTTCAGA
  TGAGACCAATTTATGCAATGGTAAGCCAGTGGATGGACTGACTACGCTGCGCAATGGGACATTAGTTGCA
  TTTCGAGGTCATTATTTCTGGATGCTGAATCCATTCAGACCACCATCTCCACCACGCAGAATTACCGAAG
  TTTGGGGTATTCCCTCTCCTATTGACACAGTTTTTACTAGATGTAACTGTGAAGGAAAAACTTTCTTCTT
  TAAGGATTCTCAGTACTGGCGCTTCACCAATGATGTGGTAGATCCTGGGTATCCTAAACAAATTGTCAAA
  GGCTTTGGAGGACTAACAGGGAAGATAGTGGCTGCTCTTTCAATAGCCAAGTACAAGGACAGGCCTGAAT
  CTGTGTACTTCTTCAAGAGAGGTGGCAACATTCAGCAGTACACTTATAAACAGGAGCCAATGAAGAAGTG
  CACAGGGAGGCGGCCTGCCATCAACTACTCGGTGTACGGGGAGGCAGCACAGGTCAGGAGGCGCCGCTTT
  GAGCGTGCTGTTGGACCTTTCCAGACACACACCTTCAGGATCCATTACTCGGTGCCCATGAGAGTTTCTT
  ATCAAGACAAAGGTTTCCTCCACAATGAAGTCAAAGTGAGTACAATGTGGAGAGGGTTTCCAAATGTTGT
  TACTTCAGCGATAACACTGCCTAACATTAGGAAACCTGATGGCTATGATTACTACGCCTTTTCCAAAGAT
  CAATACTATAACATTGATGTGCCTACAAGAACAGCAAGAGCAATTACCACACGTTCAGGGCAGACCCTCT
  CCAAAATCTGGTACAACTGTCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021400
• Insert Size: 3666 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021400.3, NP_067375.3
• RefSeq Size: 4278 bp
• RefSeq ORF: 3666 bp
• Locus ID: 96875
• Cytogenetics: 1 G1
• Gene Summary: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by orthologous human data.