Aff1 (NM_133919) Mouse Untagged Clone

CAT#: MC223967

Aff1 (untagged) - Mouse AF4/FMR2 family, member 1 (Aff1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aff1
• Synonyms: 9630032B01Rik; Af; Af4; AW319193; Mllt; Mllt2h; R; Rob
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223967 representing NM_133919
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCCATTCCAGCTTGTACAATGAAGATAGAAACCTGCTTCGAATCAGAGAGAAGGAAAGACGCA
  ACCAAGAAGCTCACCAGGAGAAGGAGGCATTTCCCGAGAAGGCTCCCCTGTTTCCAGAGCCTTACAAGAC
  TGCAAAAGGCGATGAGCTATCAAGTCGGATCCAGACCATGCTGGGTGACTATGAGGAGATGAAGGAGTTC
  CTGAGCAGCAAGTCCCACCCCCACCGCCTGGATGGCTCTGAAGACAGGCCCGGGAAGCCCAGATATCCCT
  TAGGTCATGACAGGGGGAACGGGGCTGCATCCAGCTCCCTCCGCACACATGTCTACCACCAGCCTATCCA
  CACTTCTGCTCCCGGATCACGTCCTGTCGGTAACATTAGCCACAGTCCAAAGATGGCACAGCCAAGGATG
  GAGCCAAGTCTCCACACCAAAATCTATGATGGCCCACGTCTGACTCAAGACCACCTCAGTCAGGGACATT
  GTTCCAGAAAGTGTGACCGAAGAGCTGAAGGAGACTCTGCTCCCGAGAGGAAGCTCTCGCCCTTGATCTC
  CTCTTTGCCATCCCCGGTGCCCCCTCTATCACCTGTACATTCCAGGCTGCAGGGAACCAGCAAGGCTCAC
  AGCAGTGGCGTTAGCAGTAAAAGCTGCTGTGTGGCCAAGTCTTCCAAGGACTTGGTGGCGAAGGCCCAAG
  ATAAAGAGACTCCTCATGACGGTTTGGTGGCAGTTACCAGCCTTGGATCAGCCCCTCCTCAGCCACCTTG
  CCAGACATTTCCACCCCCTCCTCTCCCCTCAAAAAGTGCTGCAATGCAGCAGAAGCCCACGGCATATGTC
  CGTCCCATGGACGGTCAGGATCAGGCTCCCAGTGAGTCCCCCGAGCTGAAGCTGCCACTGGAGGACTATG
  GTCAGCAAAGCTTTGAGAAACCAGACCTTAAAGTGCCTGCCAAAGCCAAGCTCACCAGACTAAGGATGCC
  CTCTCAGTCGGTGGAGCAGCCGTACTCCAATGAAGTCCATTGCGTTGAAGAGATTCTGAAGGAAATGACC
  CACTCGTGGCCACCGCCTCTGACAGCCATACACACACCCAGTACAGCCGAGCCGTCCAGATTTCCTTTCC
  CCACAAAGGACCCTCTGCACGTCAGTCCTGCAACCCAGAGCCAAAAACAATATGACACGCCTTCAAAAAC
  CCACCCCAATCCCCAGCAAGGAACCTCCATGCTGGAGGACGACCTGCAGCTCAGCGACAGTGAAGACAGC
  GACACGGAGCAAGCCACAGAGAAGCCTCCATCCCCACCTGCACCTCCCAGCGCTCCGCAGACACTTCCCG
  AGCCCGTGGCCTCAGCACATTCCAGTAGTGGGGAGTCAGAGAGCAGCGAGTCAGACAGTTCCTCAGACTC
  TGAGAGTGAGAGCAGCTCCAGCGACAGCGAGGAGGAGGAAGAGAACGAGCCCCTGGAGACCCGGGCTCCC
  GAGCCCGAGCCTCCGACGACAAACAAATGGCAGCTGGACAACTGGTTGACCAAAGTCAACCAACCCTCAG
  TGCCCCTGGATGGCCGGGGGAGCACAGAGTCTCCACAATGGCGCCAGGAAAGTAAGGGTGTCGCTGAGGG
  CAGCAGTGACCAGCAGCATCCTGATTCCAAAGACCCTCTCCCAAAGAGCTCCAGCAAGACTCTCCGGGGT
  CCCTCTGAGGGGCCCCAGCCTGGGAAGAGGGGCTGTCCGAAATCCCCCGCTCAACAGGAACCCCCACCTC
  GGCAAACCGTGGGCAGCAAACAACCCAGGAAACCCGCCAAGGGCTCCGGCCAGGCAGAGCCCCAGGCCAG
  CTCGCAGGCAGAGAGTGAAGTAGGACCCCTTCCCTACGGCTCAAAGGAGCAGACTTCCAAAGACAGACCC
  AAGGTGAAGACGAAAGGCAGGCCGCGTGCCGTGGGCAGTCGGGAGCCTAAGCCAGAGGTCCCTGCGCCCA
  CGCCTCAGGCAGCCGTGCCCAGACCGAAGCCGCCTGTGCCCACCCCCAGTGAGAAGAGGAAGCACAAGAG
  CTCCACGGCCCCCTCCAAGGCGCCCTCAGCCCCCCAGCCTCCGAAGGACAGTGCTGGGGACAGGAACCCA
  GAACACTCTGCTCTTGTCTCCTTGACTCAGAGCCAGGGTCCATCCCACAGCAGCAGGGGCAGCAGTGGCA
  GCGTCAGGACTAGTGGCTGCCGACAGGCTGTGATTGCCCAAGGGGACGGCTGTAAAGACAAGCTCCTGTT
  GCCTTTGAGAGACACCAAATTGCTCTCGCCGCTCAGGGACTCCCCACCACCGACAAGCTTGGTGGTGAAG
  ATAACCCTAGACCTCCTCACGAGGATTCCCCAGCCTCTGGGGAAGGGGAGCCGCCCCAGGAAAGCAGAAG
  ACAAGCAGCTGTCAGCAGGGAAGAAGCAGGACTCGGAGACCAAGAGCTGTGACAGCTCCAGCAGAGTGAC
  CAAGAAGAGAAAGGGTGACCCAGAAAAAGAGCACAGTAACAAGAGACACAAACTGGATAAGTCGCAGACT
  GCGTCATCTTCATCTTCCCATACAGAATCTTCCAGAACAAAAGCGCCCAGGTCCTCCTCAGAGAACTCAA
  GAAAGGAAATGCTGCCTCCAGCATCGGCGTCCTCAGTCTCCTCTTCCTCATCCTCCCAGAAGCCAAGCAG
  ACCTGCACAGAAGAGGCCAAGGCCAGACGAAGACACGTGTAGCCAGGAACCCCCCAGAAGTGCCAGCAGC
  ACAAAGAGCAGCTCTACAGACCCTCCCGCTCCCAAGCACAGAAAAGTACAGGCCAGGGGCTCTGAACACA
  AGGGATCTTCTGGGGATGCTGCGAATGCTGCAAATCCTTTTCCAGTGCCTTCTTTGCCGAATGGTAACGC
  TAAACCAGGGAAGCCACAGGTGAAGTCTGACAGACAACAAGCTGACTTTCACATGAAGGAGGCTAAAAAG
  CTGAAGTGCAAAGCAGAGACAATGGTAGACAAGGCTGGAAAAGCCTTCAAGTATTTGGAAGCCGTCCTCT
  CTTTTATCGAGTGTGGAATGGCCTCAGAGTCAGAAAGCTCAGCCAAGTCGGCGTACGCTGTGTACTCAGA
  AACCATAGACCTCATTAGGTATGTGATGTCACTAAAATGCTTCTCAGATAACACGATGCCAGCACAAGAG
  AAGATATTTGCTGTTTTATGCTTGCGTTGCCAGTCCCTTTTGAACATGGCGATGTTTCGATGTAAGAAAG
  ACACAGTGATGAAGTATTCTCGTACTCTCAGTGAACACTTCAAGAGTACTTCCAAAGTGGCCCAGGCACC
  TTCTCCATGCACGGCAAGAAGCACAGGCGTTCCGTCCCCCCTCTCCCCAATGCCTTCTCCTGCCAGCTCC
  GTGGGGTCCCAATCAAGTGCTGGCAGCAGTATGGGGAGCGTCGGGGTGACGGCCACCGTCAGTACTCCAG
  TCTCCATCCAGAACATGACCTCCTCCTATGTCACCATCACATCCCATGTCCTTACGGCCTTTAGCCTTTG
  GGAACAGGCCGAGGCCCTCACAAGGAAGAACAAAGAATTCTTCGCTCAGCTCAGCACAAAAGTGCGTGTG
  TTGGCCCTCAACAGCAGCCTGGTGGACCTGGTGCACTACACAAGACAGGGCCTGCAGCGGCTAAAACAGT
  CACCCAAAGGCCCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133919
• Insert Size: 3657 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133919.4, NP_598680.3
• RefSeq Size: 8339 bp
• RefSeq ORF: 3657 bp
• Locus ID: 17355
• Cytogenetics: 5 50.45 cM
• Gene Summary: This gene encodes a member of the AF4/ lymphoid nuclear protein related to the Fragile X E syndrome (FRAXE) family of proteins, which have been implicated in human childhood lymphoblastic leukemia, fragile chromosome X intellectual disability, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein domain, and a C-terminal homology domain. Knockout of the mouse gene by homologous recombination severely affects early events in lymphopoiesis, including precursor proliferation or recruitment, but is dispensable for terminal differentiation. In addition, an autosomal dominant missense mutation results in several phenotypes including ataxia and adult-onset Purkinje cell loss in the cerebellum, indicating a role in Purkinje cell maintenance and function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]
  Transcript Variant: This variant (2) uses an alternate 5'-terminal exon, which results in a different 5' UTR and use of an alternate start codon. It encodes isoform 2, which has a distinct N-terminus compared to isoform 1.