Mov10l1 (NM_031260) Mouse Untagged Clone

CAT#: MC223893

Mov10l1 (untagged) - Mouse Moloney leukemia virus 10-like 1 (Mov10l1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mov10l1
• Synonyms: CHAMP; Csm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223893 representing NM_031260
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGACGACTTGATCTACTTTTCCAATGACGCTGTGACGAGTAAAGTGCTTCTGAACGTGGGACAGG
  AAGTCATTGCTGTCGTTGAAGAAAACAAAGTGTCAAATGGACTGAAAGCAATCAGAGTAGAAGCTGTCTC
  TGACAAATGGGAAGATGATAGCAAAAACTCTAGCAAAGGGTTGTCAGACTCCAGCCCCAGAGTGCTGATT
  GGCTGTGTGACTTCCATGTTGGAAGGTGCTGGCTATATCAGCCAGACCACATACTTCTCTTTGGAGAGTG
  TGTGTGAAGGTTTCCACCCATGCAAGGGTGACTGGGTAGAGGCTGAGTATTGGATCAGGCCAGGGACATG
  GAGCAGTGAGGCAATCTCTGTGAAGCCTCTGAGGTACAAGCGTGTGGACAAGGTTTGCATTTCCAGCCTG
  TGTGGGAGGAACGGGGTGATAGAGGACAGCATCTTCTTCAGCCTGGACTCCTTGAAGCTGCCGGAAGGGT
  ACATACCGAGGAGACACGACATTGTCAATGCTGTGGTTGTGGAGAGCAGCCAGTCATGCTACATCTGGAG
  AGCACTGTGCATGACCCCTGTGAAGAGAGATGCCACTCTTGGTGAGGCCCCTCAGGAGCCCTATGGAGCA
  CTCTTACTGAAAAACAAAGGGGACATTGAAGTTACAAGAATGACCAGTTTTGGAACATTGAAGGAAGGAG
  AAAGCAAATCAATCGTGATCTGGATAGAGAATAAAGGGAAGGTCTCTCGGGAGCTTGTCAGTTGCAGACT
  GGCTAACTGGGATAAAGCACACCAGTTTAGATTTGAGACACAGGGCAGAAGCAAGTCCTGCCCAGGAGCG
  GCTGCTGGGTCTGTTCCTGAAGGTGAAAATGTTAATTCATTGAATCATCACAGAGAAGACAAAACTGATG
  AGATTCCAGAGAGCCGTCTGGCGAACAGCACAGAAATCTCTCCAGATGGCTGCGCTTGTAAAGAAGAAAG
  TAGAGAAAAAGGAAACACGCCAGAGAAACAGGAGCCAGAGCCTGGGGGGCTCATTCCTCCGGGGGAGAAG
  ACTCACATTGTGGTCACATGCAGTGCCAAAAACCCTGGCCGTTGCAAGGAGCTGCTTCTGCTCTGTTTCT
  CCGACTTTCTCATTGGGCGGCATCTTGAAGTGAGTGTGGTGAGCAGCGAGGAGGCCCTGATAGCTGTGCG
  TGAGCCGTTTTCTTGGAAGAAGCCTAAAAGCTCCCAAACATTAGTGTCTGCAAAGACTACAGTTGTTGTA
  ACCACACAAAAAAGGAACTCGAGGCGACAACTTCCAAGTTTTCTTCCACAGTATCCAATACCAGATAGAC
  TTAAAAAATGTGTGGAGCAGAAGATTGACATCCTGACTTTCCAGCCGCTTCTTGCAGAGCTCTTGAACAT
  GTCAAACTACAAGGAGAAGTTCTCCACCCTGCTGTGGCTAGAGGAGATCCATGCAGAAATCGAGCTGAAG
  GAGTACAACATGAGCAGAGTTGTCCTCAAGAGGAAGGGGGATCTGCTGGTCCTGGAGGTCCCCGGGCTCG
  CAGAGAGCCGGCCTTCCCTCTATGCAGGTGACAAACTGATTTTAAAATCTCAAGAATACAATGGACATGT
  CATTGAATATATCGGCTATGTCATGGAGATTCATGAAGAAGATGTAACTCTTAAACTTAATCCAGGATTT
  GAACAAATGTATAATTTTGAACCTATGGATGTGGAGTTTACATACAATCGGACCACAAGCAGACGGTGTC
  ACTATGCACTTGAGCAGGTCATCCATTTGGGTGTAAAAGTATTATTTCCAGAAGAAATCATTTTACAGTC
  TCCTCAGGTGACAGGGAATTGGAGCCTTGCACAGGACACCAAAAATGATGGGCAGTCCATCACCAACATC
  ACCAGAAATGATGGACAGTCCATGACCAAGGTAACCAGAAATGACAGCCAGTCCATCACCAACATCATCA
  GAAATGATGGACAGTCCATCACCAACGTCACCAGAAATGACGGGCAGCCCATCACCAAGGTAACCAGAAA
  TAACAGCCAGTCAATCACCAACATCACCAGAAATGACGGGCAGCCCATCACCAAGAACAAGAAAACAGTG
  AAGGACCAAACTAAACACACAACAGAGGAAAGGCACGTGGGTACCACGGACCAGCCAGAGAAGGCTTCCT
  CCACTGCAGAGACTATGGATGAAATCCAGATCCCAAAAGCACGAGATAAGGAGTTCTTCAACCCAGTGCT
  CAATGAAAACCAAAAGCTGGCCGTGAGGAGGATCCTGAGTGGCGACTGCCGGCCTCTCCCATATATCCTT
  TTTGGACCTCCGGGAACTGGAAAGACTGTGACTATAATCGAGGCTGTTTTGCAGGTACATTATGCTTTGC
  CGGACAGTCGGATTTTGGTCTGCGCTCCTTCCAACAGTGCTGCTGACCTTGTGTGTTTGCGACTTCATGA
  GAGCAAGGTGCTGAAGCCAGCTGCCATGGTCCGGGTGAATGCCACCTGCAGATTTGAAGAGACTATTATT
  GATGCCATCAAACCGTATTGCAGAGATGGAGAAGATATCTGGAGAGCCTCACGCTTCAGGATAATAATCA
  CTACATGTAGCAGTGCAGGACTGTTTTACCAAATAGGAGTGAGAGTTGGATACTTCACACATGTATTTGT
  GGACGAGGCAGGACAGGCAAGTGAGCCAGAATGCCTTATTCCTTTGGGACTGATTTCAGACATCAATGGC
  CAGATCGTGCTTGCTGGAGACCCCATGCAGCTCGGCCCAGTCATCAAGTCCAGGCTGGCCATGGCCTATG
  GGTTGAATGTGTCCATGTTGGAGAGGCTGATGTCCAGACCAGCGTACCTGAGAGACGAAAATGCCTTTGG
  CGCTTGCGGTGCATATAACCCATTGTTGGTCACAAAGCTTGTGAAGAACTACAGGTCCCACTCGGCTCTG
  CTGGCACTGCCCTCACGCCTGTTCTACCATAGGGAGCTTGAGGTCTGTGCTGATCCCAAAGTAGTGACTT
  CACTGCTGGGCTGGGAGAAGCTGCCCAGAAAAGGCTTCCCTCTCATCTTCCATGGAGTGAGGGGGAACGA
  GGCTCGTGAAGGGAGAAGCCCATCGTGGTTCAGCCCAGCCGAGGCTGTCCAGGTCATGCGCTACTGTTGC
  CTCTTGGCCCGGAGTGTCTCCAGTCAAGTGTCTTCCAAGGATATAGGTGTCATCACACCCTATCGGAAGC
  AGGTGGAAAAAATAAAAATCCTTCTGCGAAATGTGGATTTGACTGACATAAAGGTTGGCTCGGTAGAGGA
  GTTCCAGGGACAAGAGTACCTGGTCATCGTCATCTCCACTGTGCGGTCAAATGAAGATAGATTTGAAGAT
  GACCGTTATTTTTTGGGTTTCTTGTCCAATTCAAAAAGATTTAATGTTGCAATCACAAGACCCAAAGCAC
  TGCTGATCATTCTGGGAAACCCTCATGTGCTTGTCAGAGATCCCTGTTTTGGAGCGCTGCTAGAATACAG
  TGTTAGCAATGGTGTCTACACAGGGTGTGATCTGCCTCCTGAACTCCAGGCTCTCCAAAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031260
• Insert Size: 3564 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031260.2, NP_112550.2
• RefSeq Size: 4053 bp
• RefSeq ORF: 3564 bp
• Locus ID: 83456
• UniProt ID: Q99MV5
• Cytogenetics: 15 E3
• Gene Summary: Isoform 1: ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for germline integrity (PubMed:20534472, PubMed:20547853, PubMed:23166510, PubMed:25762440). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:20534472, PubMed:20547853, PubMed:23166510, PubMed:25762440). Involved in the primary piRNA metabolic process (PubMed:20534472, PubMed:20547853, PubMed:23166510, PubMed:25762440). Specifically binds to piRNA precursors and promotes the generation of intermediate piRNA processing fragments that are subsequently loaded to Piwi proteins (PubMed:25762440). Acts via its ATP-dependent RNA helicase activity: displays 5'-3' RNA unwinding activity and probably mediates unwinding and funneling of single-stranded piRNA precursor transcripts to the endonuclease that catalyzes the first cleavage step of piRNA processing to generate piRNA intermediate fragments that are subsequently loaded to Piwi proteins (PubMed:25762440).[UniProtKB/Swiss-Prot Function]