Pcdh15 (NM_001142747) Mouse Untagged Clone

CAT#: MC223818

Pcdh15 (untagged) - Mouse protocadherin 15 (Pcdh15), transcript variant M, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pcdh15
• Synonyms: av; BB078305; ENSMUSG00000046980; Gm9815; nmf19; roda; Ush1f
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223818 representing NM_001142747
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCTACAGTTTGCTGTCTGGAAGTGTTTACCCCATGGGATCCTCATTGCCTCTCTCTTGGTAGTCA
  GCTGGGGCCAGTATGACGATGACTGGCAATACGAGGATTGCAAACTAGCTAGGGGAGGACCACCAGCTAC
  TATCGTGGCCATTGATGAAGAGAGTCGAAACGGTACAATTCTGGTGGATAACATGTTGATTAAGGGGACT
  GCCGGAGGACCAGACCCCACCATAGAGCTCTCTTTAAAGGACAACGTGGACTACTGGGTGTTGCTGGACC
  CCGTTAAACAGATGCTTTTCCTGAACAGTACCGGAAGAGTTCTGGATAGAGACCCACCAATGAACATACA
  CTCCATTGTGGTGCAAGTCCAGTGTGTCAACAAGAAGGTTGGCACAGTTATCTATCATGAAGTACGCATC
  GTGGTGCGAGATCGGAATGACAACTCCCCCACATTCAAGCATGAAAGCTACTATGCCACCGTGAATGAGC
  TCACTCCAGTTGGCACCACGATATTCACGGGGTTCTCGGGAGACAATGGAGCTACAGACATAGACGATGG
  CCCTAATGGACAGATAGAATACGTGATTCAGTACAACCCAGAAGATCCGACATCCAACGACACCTTTGAA
  ATTCCACTCATGCTGACTGGCAACGTGGTACTGAGGAAAAGACTCAACTATGAGGATAAGACTCGCTACT
  ATGTCATCATCCAAGCAAATGACCGTGCACAAAATCTGAATGAGAGGCGAACAACCACCACCACCCTCAC
  AGTAGATGTTCTAGATGGAGATGACCTGGGACCTATGTTTCTGCCTTGTGTTCTTGTGCCAAACACACGT
  GACTGTCGTCCACTCACCTACCAAGCTGCCATTCCTGAACTGAGGACTCCGGAAGAACTGAACCCTATTT
  TGGTGACACCACCTATCCAAGCCATTGATCAGGACCGAAACATCCAACCACCATCTGATCGACCTGGCAT
  CCTCTACTCCATCCTTGTCGGCACCCCTGAGGATTACCCCCGCTTCTTCCATATGCATCCCAGGACTGCA
  GAACTCACTCTCCTGGAGCCAGTAAACAGAGACTTCCATCAAAAATTTGATTTGGTTATTAAGGCTGAGC
  AGGACAATGGCCACCCACTTCCTGCCTTTGCTAGTCTGCACATCGAAATACTAGACGAAAACAATCAGAG
  TCCATACTTCACAATGCCCAGCTATCAAGGATACATCCTGGAATCCGCCCCAGTGGGAGCCACCATTTCT
  GAGAGCCTAAACTTAACCACTCCTCTGAGAATTGTAGCTCTGGACAAAGACATAGAAGACACAAAAGATC
  CAGAGCTCCACCTCTTCCTGAATGACTACACCTCGGTCTTCACTGTGACACCCACTGGTATCACCCGCTA
  CCTCACCCTGCTTCAACCTGTGGACAGGGAGGAACAGCAAACCTACACCTTTCTGATAACAGCGTTTGAT
  GGCGTGCAAGAAAGTGAGCCAGTCGTGGTCAATATCCGAGTGATGGATGCAAATGATAACACGCCCACCT
  TCCCTGAAATCTCCTATGATGTCTATGTTTACACAGACATGAGTCCTGGGGACAGCGTCATTCAGCTGAC
  AGCGGTAGATGCTGATGAAGGCTCTAATGGGGAGATCTCCTATGAAATACTGGTGGGGGGCAAGGGAGAC
  TTCGTGATCAACAAGACCACAGGGCTGGTGAGCATTGCACCAGGCGTGGAGCTGATCGTGGGACAGACGT
  ATGCGCTCACAGTGCAGGCTTCGGACAACGCCCCGCCTGCAGAAAGAAGGCACTCCATCTGCACAGTGTA
  CATCGAGGTGCTTCCTCCTAACAACCAGAGCCCTCCCCGCTTCCCGCAGCTGATGTACAGTCTGGAAGTC
  AGCGAGGCCATGAGGATCGGTGCTATTTTATTAAATCTACAGGCAACTGATCGAGAGGGAGATCCAATCA
  CATATGCCATCGAGAATGGAGACCCTCAGAGAGTTTTTAATCTTTCAGAAACCACAGGGATTCTCAGCCT
  AGGGAAGGCTCTAGACCGCGAGAGCACAGACCGCTACATCCTCATCGTCACAGCCTCAGATGGCAGACCG
  GATGGAACCTCAACTGCCACTGTGAACATAGTGGTGACGGACGTCAATGACAACGCTCCCGTGTTCGATC
  CCTATCTGCCCAGGAACCTCTCTGTGGTGGAGGAAGAAGCCAATGCCTTTGTGGGTCAAGTCCGGGCAAC
  AGACCCAGATGCTGGGATAAACGGCCAAGTTCACTACAGCCTGGGGAACTTCAACAACCTCTTCCGCATC
  ACATCCAACGGGAGCATTTACACAGCCGTGAAGCTGAACAGGGAAGCCAGGGACCACTATGAACTGGTTG
  TCGTGGCAACAGATGGAGCAGTCCACCCTCGACATTCAACTCTGACACTGTACATCAAGGTGTTGGACAT
  TGATGATAACAGTCCTGTTTTTACCAATTCAACGTACACAGTTGTCGTTGAAGAGAATCTGCCAGCCGGG
  ACCTCCTTTCTTCAAATAGAGGCCAAGGATGTTGACCTTGGAGCCAATGTGTCATATCGGATCAGAAGCC
  CAGAAGTGAAACACCTTTTTGCACTGCATCCATTCACTGGAGAATTGTCTCTTCTGAGGAGTTTGGATTA
  TGAGGCCTTTCCGGACCAGGAGGCAAGCATCACATTCTTGGTGGAGGCCTTTGACATTTATGGGACTATG
  CCACCTGGTATAGCAACAGTCACGGTAATTGTGAAGGACATGAATGACTACCCTCCAGTGTTTAGCAAAC
  GCATCTACAAGGGGATGGTGGCTCCAGATGCAGTCAAGGGGACACCAATCACCACCGTTTATGCTGAAGA
  TGCGGACCCACCTGGGATGCCTGCAAGTAGGGTGAGGTATCGAGTGGACGACGTGCAGTTTCCATACCCA
  GCCAGTATTTTTGATGTAGAGGAAGATTCTGGAAGAGTAGTAACCCGCGTCAATCTTAATGAAGAGCCTA
  CTACGATTTTCAAGCTGGTGGTTGTGGCTTTTGATGACGGCGAACCTGTGATGTCCAGCAGTGCCACGGT
  GAGAATTCTTGTCTTACATCCTGGAGAGATCCCACGCTTCACCCAAGAGGAATACAGACCTCCTCCTGTA
  AGTGAGCTTGCGGCCAGAGGGACTGTAGTTGGTGTCATTTCTGCTGCTGCCATTAATCAGAGCATCGTGT
  ACTCCATTGTGGCAGGAAATGAGGAAGACAAGTTTGGAATCAACAATGTCACTGGGGTCATCTATGTGAA
  TTCACCATTGGATTACGAGACAAGGACCAGCTATGTGCTCCGGGTACAAGCAGATTCTCTGGAAGTGGTC
  CTTGCCAATCTCCGAGTCCCTTCAAAAAGTCTGTCTGTGATACCATGTTCATGGAGAACCCAGGTGTCAA
  AGTCTCTAGGACTGGAGCTAGGGGTGCCTGTGAGCCACAGTGTGGAATCTGGAACTAGAACTGGGTCCTC
  TACAAGAGCAGCAAGTGTTCCTATCCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142747
• Insert Size: 3531 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142747.1, NP_001136219.1
• RefSeq Size: 4954 bp
• RefSeq ORF: 3531 bp
• Locus ID: 11994
• UniProt ID: Q99PJ1
• Cytogenetics: 10 37.43 cM
• Gene Summary: Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (M) has a distinct 3' splice pattern, compared to variant A. The resulting isoform (SI-1), also known as protocadherin-15-secreted isoform 1, has a substantially shorter and distinct C-terminus, compared to isoform CD1-1.