Tnks2 (NM_001163635) Mouse Untagged Clone

CAT#: MC223799

Tnks2 (untagged) - Mouse tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 (Tnks2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tnks2
• Synonyms: 5430432P15Rik; AA517131; AI662480; ARTD6; Tank2; TNKS-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223799 representing NM_001163635
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGGCCGCCGCTGCGCCGGCGGGGGCGCGGCCTGCGCGAGCGCCGGGGCCGAGGCCGTGGAGCCGT
  CCGCCCGCGAGCTGTTCGAGGCTTGCCGCAACGGGGACGTGGAGCGAGTCAAGAGGCTGGTGACGCCCGA
  GAAGGTGAACAGCCGCGACACGGCGGGCAGGAAGTCCACCCCGCTCCACTTCGCCGCAGGTTTTGGACGG
  AAGGATGTAGTTGAATATCTTCTTCAGAACGGTGCAAATGTGCAAGCGCGTGATGATGGGGGTCTTATTC
  CTCTTCATAACGCATGCTCTTTTGGTCATGCTGAAGTAGTCAATCTTCTTTTACAACACGGTGCAGACCC
  AAATGCTCGGGATAATTGGAATTATACTCCTCTCCATGAAGCTGCAATTAAAGGAAAAATTGATGTTTGC
  ATTGTGCTTTTACAGCATGGAGCTGAGCCAACCATCCGAAATACAGATGGAAGGACAGCATTGGATTTAG
  CAGACCCATCTGCCAAAGCTGTTCTGACTGGTGACTATAAGAAAGATGAACTTCTGGAAAGTGCCAGGAG
  TGGCAATGAGGAAAAAATGATGGCCCTGCTTACACCATTGAATGTCAACTGCCATGCAAGTGATGGCAGA
  AAGTCAACTCCATTGCATTTGGCAGCAGGATACAACAGAGTAAAAATTGTACAACTGTTACTGCACCATG
  GGGCTGATGTCCATGCTAAAGATAAAGGGGATCTGGTACCACTACACAATGCCTGCTCTTACGGTCATTA
  TGAAGTAACTGAACTTCTGGTCAAGCACGGTGCCTGTGTGAATGCAATGGACCTGTGGCAGTTCACTCCT
  CTTCATGAGGCAGCTTCTAAGAACAGGATTGAAGTATGTTCTCTCCTCCTAAGTTACGGGGCAGATCCAA
  CCCTGCTGAACTGTCACAATAAAAGTGCTATAGACCTGGCCCCCACGGCACAGCTGAAGGAAAGATTATC
  GTATGAATTTAAAGGCCATTCATTGCTGCAAGCTGCACGAGAAGCTGATGTTACAAGAATCAAAAAACAT
  CTTTCTCTGGAAATGGTGAATTTCAAACATCCTCAGACACATGAAACAGCACTGCATTGTGCTGCTGCAT
  CTCCATATCCCAAAAGAAAGCAGATATGTGAACTGCTGCTCAGAAAGGGAGCAAACACCAACGAAAAGAC
  TAAAGAATTCTTGACTCCTCTGCACGTGGCTTCTGAGAACGCTCACAATGATGTTGTTGAAGTAGTGGTG
  AAACATGAAGCAAAGGTTAATGCTTTGGATAGTCTTGGACAGACGTCATTACACAGAGCTGCACACTGTG
  GTCACCTGCAGACCTGCCGCCTGCTCCTGAGCTATGGGTGTGATCCAAACATCATATCCCTTCAGGGTTT
  CACCGCCTTGCAAATGGGAAATGAAAATGTGCAGCAGCTGCTCCAAGAGGGCGCCTCACTAGGTCACTCA
  GAGGCGGACAGACAGCTGCTGGAAGCTGCAAAGGCTGGTGACGTTGAAACTGTAAAAAAACTCTGTACTG
  TTCAGAGTGTCAACTGCAGAGACATTGAAGGACGCCAGTCAACCCCACTCCACTTTGCAGCTGGGTACAA
  CAGAGTGTCTGTGGTAGAATATCTGCTGCAACATGGAGCTGATGTTCATGCTAAAGACAAAGGAGGTCTT
  GTACCTTTACACAATGCATGTTCTTATGGACACTATGAAGTTGCAGAACTTCTTGTCAAGCATGGAGCAG
  TAGTCAATGTCGCTGACTTGTGGAAGTTCACACCTTTACATGAAGCTGCAGCAAAAGGAAAATATGAAAT
  ATGCAAACTTCTACTCCAGCATGGTGCAGACCCTACAAAGAAAAACAGAGATGGTAATACTCCTCTGGAT
  CTTGTTAAAGATGGAGATACAGATATTCAAGATCTACTTAGAGGTGATGCAGCTTTGCTGGATGCTGCCA
  AGAAGGGTTGTTTAGCCAGAGTGAAGAAATTGTCTTCTCCTGATAATGTAAATTGCCGTGATACCCAAGG
  TCGGCATTCTACACCTTTACATTTGGCAGCTGGTTATAATAACTTGGAAGTTGCTGAGTATTTATTGCAA
  CATGGAGCTGATGTGAATGCCCAGGACAAAGGAGGACTCATTCCTTTACATAACGCAGCATCGTATGGAC
  ATGTAGATGTAGCAGCTTTGCTGATAAAGTACAATGCGTGTGTCAATGCCACGGACAAATGGGCTTTCAC
  ACCGTTACATGAAGCAGCCCAAAAGGGACGGACACAGCTTTGTGCTTTATTGTTGGCCCATGGAGCTGAT
  CCTACTCTTAAAAATCAAGAAGGACAAACACCTTTAGATTTAGTTTCAGCAGATGATGTCAGTGCACTCT
  TGACAGCAGCCATGCCCCCCTCTGCTCTGCCTACGTGCTACAAACCTCAAGTGCTCAGTGGCGTGAGGGG
  CCCCGGAGCCACTGCAGATGCTCTGTCTTCAGGTCCGTCCAGCCCATCCAGCCTCTCTGCAGCCAGCAGC
  CTCGACAACTTATCTGGCAGCTTCTCGGAACTGTCCGCAGTGGTTAGTTCAAGTGCAGCAGAAGGTGCTA
  CTGGTTTGCAAAGAAAAGAGGATTCAGGAATCGATTTTAGTATAACTCAGTTCATAAGGAACCTTGGACT
  TGAGCACTTGATGGATATATTTGAGAGAGAACAGATTACCTTAGATGTCCTAGTTGAAATGGGCCACAAG
  GAACTGAAAGAGATTGGAATCAATGCCTATGGACATCGACACAAGCTGATTAAAGGAGTTGAAAGGCTGA
  TCTCTGGACAACAAGGTCTTAATCCATATTTAACTCTAAACAACTCTGGTAGTGGAACAATTCTCATAGA
  TCTGTCTCCTGATGATAAAGAATTTCAGTCTGTGGAAGAAGAGATGCAGAGTACTGTGCGGGAGCACAGA
  GATGGTGGTCACGCAGGCGGCGTCTTCAACAGATACAACATTCTCAAGATTCAGAAGGTTTGTAACAAGA
  AATTGTGGGAAAGATATACACACCGGAGAAAAGAAGTTTCTGAAGAAAACCACAACCATGCAAACGAAAG
  GATGTTATTTCATGGGTCTCCTTTTGTGAATGCGATTATCCATAAGGGCTTTGATGAAAGGCATGCATAC
  ATAGGTGGCATGTTTGGAGCTGGAATTTATTTTGCTGAGAACTCTTCCAAAAGCAATCAATATGTGTATG
  GAATTGGAGGTGGCACCGGATGTCCAATTCACAAAGACAGATCGTGTTACATTTGTCACAGGCAGCTGCT
  GTTTTGTCGAGTAACCTTGGGAAAGTCTTTCTTGCAGTTCAGCGCAATGAAAATGGCACATTCTCCTCCC
  GGCCATCACTCGGTCACTGGCCGGCCCAGTGTAAATGGCCTAGCATTAGCTGAATATGTTATTTACAGAG
  GAGAACAGGCTTATCCTGAATATCTAATTACTTACCAGATTGTAAGGCCTGAAGGTATGGTTGATGGCTG
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163635
• Insert Size: 3501 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163635.1, NP_001157107.1
• RefSeq Size: 6503 bp
• RefSeq ORF: 3501 bp
• Locus ID: 74493
• UniProt ID: Q3UES3
• Cytogenetics: 19 C2
• Gene Summary: Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length. Stimulates 26S proteasome activity.[UniProtKB/Swiss-Prot Function]