Lepr (NM_146146) Mouse Untagged Clone

CAT#: MC223793

Lepr (untagged) - Mouse leptin receptor (Lepr), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lepr
• Synonyms: db; diabetes; Leprb; LEPROT; Modb1; OB-RGRP; obese-like; obl; Obr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223793 representing NM_146146
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGTCAGAAATTCTATGTGGTTTTGTTACACTGGGAATTTCTTTATGTGATAGCTGCACTTAACC
  TGGCATATCCAATCTCTCCCTGGAAATTTAAGTTGTTTTGTGGACCACCGAACACAACCGATGACTCCTT
  TCTCTCACCTGCTGGAGCCCCAAACAATGCCTCGGCTTTGAAGGGGGCTTCTGAAGCAATTGTTGAAGCT
  AAATTTAATTCAAGTGGTATCTACGTTCCTGAGTTATCCAAAACAGTCTTCCACTGTTGCTTTGGGAATG
  AGCAAGGTCAAAACTGCTCTGCACTCACAGACAACACTGAAGGGAAGACACTGGCTTCAGTAGTGAAGGC
  TTCAGTTTTTCGCCAGCTAGGTGTAAACTGGGACATAGAGTGCTGGATGAAAGGGGACTTGACATTATTC
  ATCTGTCATATGGAGCCATTACCTAAGAACCCCTTCAAGAATTATGACTCTAAGGTCCATCTTTTATATG
  ATCTGCCTGAAGTCATAGATGATTCGCCTCTGCCCCCACTGAAAGACAGCTTTCAGACTGTCCAATGCAA
  CTGCAGTCTTCGGGGATGTGAATGTCATGTGCCGGTACCCAGAGCCAAACTCAACTACGCTCTTCTGATG
  TATTTGGAAATCACATCTGCCGGTGTGAGTTTTCAGTCACCTCTGATGTCACTGCAGCCCATGCTTGTTG
  TGAAACCCGATCCACCCTTAGGTTTGCATATGGAAGTCACAGATGATGGTAATTTAAAGATTTCTTGGGA
  CAGCCAAACAATGGCACCATTTCCGCTTCAATATCAGGTGAAATATTTAGAGAATTCTACAATTGTAAGA
  GAGGCTGCTGAAATTGTCTCAGCTACATCTCTGCTGGTAGACAGTGTGCTTCCTGGATCTTCATATGAGG
  TCCAGGTGAGGAGCAAGAGACTGGATGGTTCAGGAGTCTGGAGTGACTGGAGTTCACCTCAAGTCTTTAC
  CACACAAGATGTTGTGTATTTTCCACCCAAAATTCTGACTAGTGTTGGATCGAATGCTTCTTTTCATTGC
  ATCTACAAAAACGAAAACCAGATTATCTCCTCAAAACAGATAGTTTGGTGGAGGAATCTAGCTGAGAAAA
  TCCCTGAGATACAGTACAGCATTGTGAGTGACCGAGTTAGCAAAGTTACCTTCTCCAACCTGAAAGCCAC
  CAGACCTCGAGGGAAGTTTACCTATGACGCAGTGTACTGCTGCAATGAGCAGGCGTGCCATCACCGCTAT
  GCTGAATTATACGTGATCGATGTCAATATCAATATATCATGTGAAACTGACGGGTACTTAACTAAAATGA
  CTTGCAGATGGTCACCCAGCACAATCCAATCACTAGTGGGAAGCACTGTGCAGCTGAGGTATCACAGGCG
  CAGCCTGTATTGTCCTGATAGTCCATCTATTCATCCTACGTCTGAGCCCAAAAACTGCGTCTTACAGAGA
  GACGGCTTTTATGAATGTGTTTTCCAGCCAATCTTTCTATTATCTGGCTATACAATGTGGATCAGGATCA
  ACCATTCTTTAGGTTCACTTGACTCGCCACCAACGTGTGTCCTTCCTGACTCCGTAGTAAAACCACTACC
  TCCATCTAACGTAAAAGCAGAGATTACTGTAAACACTGGATTATTGAAAGTATCTTGGGAAAAGCCAGTC
  TTTCCGGAGAATAACCTTCAATTCCAGATTCGATATGGCTTAAGTGGAAAAGAAATACAATGGAAGACAC
  ATGAGGTATTCGATGCAAAGTCAAAGTCTGCCAGCCTGCTGGTGTCAGACCTCTGTGCAGTCTATGTGGT
  CCAGGTTCGCTGCCGGCGGTTGGATGGACTAGGATATTGGAGTAATTGGAGCAGTCCAGCCTATACGCTT
  GTCATGGATGTAAAAGTTCCTATGAGAGGGCCTGAATTTTGGAGAAAAATGGATGGGGACGTTACTAAAA
  AGGAGAGAAATGTCACCTTGCTTTGGAAGCCCCTGACGAAAAATGACTCACTGTGTAGTGTGAGGAGGTA
  CGTGGTGAAGCATCGTACTGCCCACAATGGGACGTGGTCAGAAGATGTGGGAAATCGGACCAATCTCACT
  TTCCTGTGGACAGAACCAGCGCACACTGTTACAGTTCTGGCTGTCAATTCCCTCGGCGCTTCCCTTGTGA
  ATTTTAACCTTACCTTCTCATGGCCCATGAGTAAAGTGAGTGCTGTGGAGTCACTCAGTGCTTATCCCCT
  GAGCAGCAGCTGTGTCATCCTTTCCTGGACACTGTCACCTGATGATTATAGTCTGTTATATCTGGTTATT
  GAATGGAAGATCCTTAATGAAGATGATGGAATGAAGTGGCTTAGAATTCCCTCGAATGTTAAAAAGTTTT
  ATATCCACGATAATTTTATTCCCATCGAGAAATATCAGTTTAGTCTTTACCCAGTATTTATGGAAGGAGT
  TGGAAAACCAAAGATAATTAATGGTTTCACCAAAGATGCTATCGACAAGCAGCAGAATGACGCAGGGCTG
  TATGTCATTGTACCCATAATTATTTCCTCTTGTGTCCTACTGCTCGGAACACTGTTAATTTCACACCAGA
  GAATGAAAAAGTTGTTTTGGGACGATGTTCCAAACCCCAAGAATTGTTCCTGGGCACAAGGACTGAATTT
  CCAAAAGCCTGAAACATTTGAGCATCTTTTTACCAAGCATGCAGAATCAGTGATATTTGGTCCTCTTCTT
  CTGGAGCCTGAACCCATTTCAGAAGAAATCAGTGTCGATACAGCTTGGAAAAATAAAGATGAGATGGTCC
  CAGCAGCTATGGTCTCCCTTCTTTTGACCACACCAGACCCTGAAAGCAGTTCTATTTGTATTAGTGACCA
  GTGTAACAGTGCTAACTTCTCTGGGTCTCAGAGCACCCAGGTAACCTGTGAGGATGAGTGTCAGAGACAA
  CCCTCAGTTAAATATGCAACTCTGGTCAGCAACGATAAACTAGTGGAAACTGATGAAGAGCAAGGGTTTA
  TCCATAGTCCTGTCAGCAACTGCATCTCCAGTAATCATTCCCCACTGAGGCAGTCTTTCTCTAGCAGCTC
  CTGGGAGACAGAGGCCCAGACATTTTTCCTTTTATCAGACCAGCAACCCACCATGATTTCACCACAACTT
  TCATTCTCGGGGTTGGATGAGCTTTTGGAACTGGAGGGAAGTTTTCCTGAAGAAAATCACAGGGAGAAGT
  CTGTCTGTTATCTAGGAGTCACCTCCGTCAACAGAAGAGAGAGTGGTGTGCTTTTGACTGGTGAGGCAGG
  AATCCTGTGCACATTCCCAGCCCAGTGTCTGTTCAGTGACATCAGGATCCTCCAGGAGAGATGCTCACAC
  TTTGTAGAAAATAATTTGAGTTTAGGGACCTCTGGTGAGAACTTTGTACCTTACATGCCCCAATTTCAAA
  CCTGTTCCACGCACAGTCACAAGATAATGGAGAATAAGATGTGTGACTTAACTGTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_146146
• Insert Size: 3489 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146146.2, NP_666258.2
• RefSeq Size: 4126 bp
• RefSeq ORF: 3489 bp
• Locus ID: 16847
• UniProt ID: P48356
• Cytogenetics: 4 46.96 cM
• Gene Summary: Receptor for hormone LEP/leptin (Probable) (PubMed:11861497). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS (PubMed:10799542, PubMed:25383904, PubMed:11923481, PubMed:11861497). In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (PubMed:10660043, PubMed:12594516). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (PubMed:25383904, PubMed:11923481). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis (PubMed:12594516). Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus (PubMed:25383904). Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T-cells. Leptin increases Th1 and suppresses Th2 cytokine production (PubMed:9732873).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.