Dgkh (NM_001081336) Mouse Untagged Clone

CAT#: MC223782

Dgkh (untagged) - Mouse diacylglycerol kinase, eta (Dgkh), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dgkh
• Synonyms: 5930402B05Rik; D130015C16; DGK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223782 representing NM_001081336
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGGCCGGCAGCCAGCACCACCCTCAGGGCGTCGCGGGAGGAGCGGTCGCTGGGGCCAGCGCGG
  TTTCCCCCACTGCTGCAGGACCGGGAGAGGATTCGTCCGACAGTGAAGCGGAGCAGGAGGGGCCCCAGAA
  GCTGATCCGTAAAGTGTCCACCTCTGGACAGATGCGGACCAAGACTAGTATTAAGGAAGGACAACTGCTG
  AAGCAAACCAGCTCTTTCCAAAGGTGGAAGAAGAGATACTTCAAGCTTCGCGGCCGCACACTTTATTATG
  CGAAAGACTCCAAGTCCTTGATATTTGATGAAGTTGACCTCTCAGATGCCAGTGTTGCGGAAGCAAGCAC
  AAAAAATGCCAACAACAGCTTTACGATCATCACTCCGTTCAGAAGACTGATGCTATGTGCTGAGAACAGG
  AAAGAGATGGAGGTCTGGATCAGCTCTCTGAAGTCAGTGCAGAGCAGAGAACCCTATGAGGTGGCCCAGT
  TCAATGTGGAACATTTCTCAGGGATGCACAACTGGTACGCCTGCTCCCACGCCCGGCCCACCTTCTGTAA
  CGTGTGCAGAGAGAGTCTCTCTGGAGTGACCTCCCACGGCCTGTCCTGTGAAGTGTGTAAGTTCAAGGCT
  CATAAAAGATGTGCAGTGAGGGCAACAAACAACTGCAAATGGACCACGCTGGCCTCCATCGGGAAGGACA
  TCATAGAGGACGAGGATGGTGTTGCCATGCCTCACCAGTGGCTCGAAGGCAACTTGCCTGTGAGTGCCAA
  GTGTGCAGTCTGTGACAAAACCTGTGGCAGTGTGCTTCGCCTGCAAGATTGGAAATGCCTTTGGTGTAAG
  ACAATGGTGCACACTGCCTGCAAAGATGTGTACCATCCCGTCTGTCCCCTTGGCCAGTGTAAAGTCTCCA
  TCATACCTCCGATTGCACTAAATAGCACAGACTCTGATGGTTTCTGTCGAGCAACCTTTTCGTTCTGTGT
  CAGCCCCCTCTTGGTGTTCGTCAACTCTAAGAGTGGAGATAATCAGGGAGTGAAGTTCCTTCGTCGCTTT
  AAACAGTTGCTCAACCCAGCTCAGGTGTTTGATTTAATGAACGGAGGGCCTTATTTAGGCTTGAGATTGT
  TTCAAAAGTTTGACAACTTTCGGATCCTTGTCTGTGGAGGAGATGGAAGTGTAGGCTGGGTTTTGTCAGA
  GATTGATAAACTGAACTTGCATAAACAGTGCCAGCTTGGGGTGCTACCCTTGGGTACCGGCAATGACCTT
  GCTCGAGTTCTTGGCTGGGGAGGTTCATATGATGATGACACCCAACTCCCTCAGATACTAGAGAAGCTGG
  AACGAGCCAGTACCAAAATGCTGGACAGGTGGAGTATAATGACCTATGAGCTCAAATTGCCAGCAAAGTC
  GTCTCTACTTCCTGAACCTGTGGCAGCAACTGAAGAATTTTACATGACAATTTATGAAGACTCCGTTGCA
  AACCATCTTACGAAAATCGTCAACTCTGATGAGCATGCGGTGGTCATATCGTCTGCCAAGATACTCTGTG
  AAACCGTGAAGGACTTCGTTGCCAAAGTGGAGAAGGCACAGGACAGAACACTGGAAAATACAGTAGTAGC
  CGAAGCCGTGGCCAGTAAATGCTCAGTCCTAAACGAGAAGCTTGAGCAGTTGCTACAAGCCTTGCACGCG
  GACTCTCAGGCCAGTCGAGTCCCCCCAGGAATCGGCCCTGCCATCCCCGAAGAGGACACCGTGGAGTCCG
  CCAGTGACGAGTCCTTGGGTGAAAGCAAGGACCAGCTGGTGAATGACATTGGGAAACCCTCCTCCCAGAA
  AGCCGTGAAGCCCCGGGAGATAATGCTGCGGGCCAACAGTCTAAAGAAAGCGGTGAGGCAAGTCATTGAA
  GAAGCTGAAAAAGTTATGGATGAGCCAGCAGTGCAGCCCTCTGAGCCAGTCAGTCCGTCTTGTGACTATG
  ATACAGAAACAGATGAAGCTAAAGAGGACGACGCGAAAGAGTCGCTATCAGCCAAAACCACATCTCAGTC
  TCCTGATGCCCAGGCAAGCTGTGGCCATCCCCAAACTGACTCTGTCGCTGGTCCAGCCATGGCCACCACC
  AAAGAAAACCTCCCTGTGCTCAACACTAGAATCATCTGCCCAGGTTTGAGAGCAGGACTTGCTGCCTCCA
  TTGCTGGGAGTTCTATCATCAACAAAATGTTGCTGGCGAACATCGATCCTTTCGGGGCAACACCGTTTAT
  TGACCCAGATCTAGACTCACTAGATGGATACTCTGAAAAATGTGTCATGAACAACTACTTTGGGATTGGA
  TTAGATGCAAAAATTTCATTAGAATTTAATAATAAGAGAGAGGAGCACCCTGAAAAATGCAGAAGTCGAA
  CTAAAAACTTGATGTGGTACGGAGTCCTTGGGACCCGGGAGCTACTACAGAGATCGTACAAGAATCTGGA
  GCAGAGGGTTCAACTTGAGTGTGATGGGCAGTATATACCTCTTCCTAGTCTACAAGGCATAGCGGTGTTG
  AACATCCCTAGCTACGCCGGAGGCACCAACTTCTGGGGCGGAACCAAAGAGGATGATATATTTGCTGCGC
  CATCCTTTGATGACAAGATTCTAGAAGTTGTCGCAGTGTTTGACAGTGTGCAGATGGCAGTATCAAGGGT
  CATTAAATTGCAGCATCACCGGATAGCACAGTGCCGTACGGTAAAAATCACGATATTTGGCGACGAGGGA
  GTCCCAGTGCAGGTGGATGGTGAAGCATGGGTCCAGCCTCCAGGGATCATCAAGATTGTGCACAAAAACA
  GAGCTCAGATGCTAACCAGAGACAGAGCCTTTGAAAGCACCCTGAAGTCTTGGGAAGACAAGCAGAAGTG
  TGACTCTGGGAAGCCAGTTCTTCGAACCAACCTGTACATCCATCCCGCCCCTGACCTGGCAACAGAGGAA
  GTGTCACAGATGCGGCTCTGCTCCCAGGCTGCGGAGGAACTCATCACTAGGATTTGTGACGCAGCCACGA
  TTCACTGTCTCCTGGAGCAGGAGCTGGCACATGCAGTGAATGCCTGCTCACACGCCCTGAACAAAGCCAA
  CCCACGCTTCCCGGAGAGCCTTACGAGAGACACTGCCACCGAGATAGCCATCAACGTCAAGGCGCTCTAT
  AATGAGACAGAGGCTTTGCTAGTTGGCAGGGTCCCTTTGCACTTGGAATCTCCTCACGAAGAGCGGGTGT
  CCAGTGCCTTACACTCTGTGGAGATGGAGCTTCAGAAGTTAACGGAGATTCCCTGGCTTTATTACATCCT
  CCGCCCCAGTGAGGACGAGGAGCCTCCTCTGGATTGTACCAAAAGAAACAACAAAAGCACAGTCTTTCGC
  ATAGTGCCAAAGTTTAAAAAGGAAAAGGCTCAGAAACAGAAAACAAGTTCGCAGCCTGGACCTGGGGATT
  CCGAAAGTGGGTCATATGAAGCGAATTCTCCAGGGAATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081336
• Insert Size: 3471 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081336.1, NP_001074805.1
• RefSeq Size: 3967 bp
• RefSeq ORF: 3543 bp
• Locus ID: 380921
• UniProt ID: D3YXJ0
• Cytogenetics: 14 D3
• Gene Summary: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:27643686). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcripts and orthologous alignments from human (NM_001204504.1).