Aff4 (NM_033565) Mouse Untagged Clone

CAT#: MC223774

Aff4 (untagged) - Mouse AF4/FMR2 family, member 4 (Aff4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aff4
• Synonyms: AF5Q31; Alf4; Laf4l; MCEF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223774 representing NM_033565
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGTGAAGACCGGAATGTGCTGCGCATGAAAGAAAGGGAAAGGCGGAATCAGGAAATTCAGCAGG
  GTGAAGACGCCTTCCCACCTAGTTCTCCTCTCTTTGCTGAGCCATACAAAGTCACTAGCAAAGAAGATAA
  GTTATCAAGTCGGATTCAGAGCATGCTTGGAAACTACGATGAAATGAAGGATTACATAGGAGACAGATCG
  ATACCAAAGCTTGTTGCAATTCCCAAGCCTGCAGTACCAACGACCACAGACGAGAAAGCAAACCCAAATT
  TTTTTGAGCAGAGACATGGAGGATCTCATCAGAGTAGCAAATGGACTCCTGTAGGCCCCGCCCCCAGCAC
  CTCTCAGTCTCAGAAACGCTCCTCTGCCCTGCAGAGTGGACACAGCAGCCAACGGAGTGGTGCAGGAGGG
  AGTGGCGCCAGCAGCAGTGGCCAGAGGCATGACCGTGACTCCTACAGCAGTAGCAGGAAAAAAGGCCAGC
  ACGGCTCAGAGCACTCCAAATCTCGATCCTCAAGCCCTGGAAAACCCCAGGCTGTTTCTTCATTAAGTTC
  TAGTCATTCCAGGTCTCATGGGAATGATCACCATAGCAAGGAACATCAGCGCTCCAAATCACCTCGGGAC
  CCTGATGCCAACTGGGATTCTCCTTCCCGCGGACCTTTTTCAAGTGGGCAACATTCAAGTCAGTCTTTCC
  CACCTTCTTTGATGTCAAAATCTAGTTCAATGTTACAGAAACCCACTGCCTATGTACGGCCCATGGATGG
  ACAGGAGTCTGTGGAACCAAAGCTGTCCTCTGAGCACTACAGCAGCCAGTCTCATGGTAATAGCATGACT
  GAGCTGAAGCCCAGCAGCAAAGCACATCTCACCAAGCTGAAAATTCCTTCCCGACCTTTGGATGCATCGG
  TTTCTGGTGATGTAAGCTGTGTAGATGAAATACTTAAAGAGATGACGCATTCATGGCCTCCCCCTCTAAC
  GGCTATTCATACACCATGCAAAACAGAACCTTCCAAATTCCCTTTTCCAACTAAGGAGTCGCAACAGTCT
  AACTTTGGCCCTGGAGAACAAAAAAGATACAGTACTGCTAAAACATCAAATGGACACCAGTCAAAATCTA
  TGTTAAAAGATGACTTAAAACTAAGCAGCAGTGAAGACAGTGATGGGGAACAGGATTGTGATAAGACAAT
  GCCAAGGAGTACACCAGGAAGTAACTCTGAACCTTCACACCATAACAGTGAGGGAGCAGACAACTCCAGG
  GATGACTCGAGCAGCCACAGTGGATCTGAGAGCAGCTCTGGGTCTGACTCGGAGAGTGAGAGCAGCTCCA
  GTGACAGTGAGGCTAATGAGCCGTCGCAGAGCGCATCTCCTGAGCCTGAACCACCACCAACAAACAAGTG
  GCAACTTGATAATTGGTTGAATAAAGTGAACCCACATAAAGTATCCCCAGCTTCCTCTGTAGACAGCAAC
  ATCCCATCTTCTCAAGCCTACAAGAAAGAAGGCCGGGAGCAGGGCACTGCCAGTAACTACACAGATCCAG
  GGGGGACAAAAGAAACGAGCTCTGCCACCCCAGGACGAGACTCTAAAACCATCCAAAAGGGATCAGAAAG
  TGGCCGTGGGAGGCAGAAGTCTCCTGCACAGAGCGACAGTACCACTCAGAGGAGAACTGTCGGCAAAAAA
  CAACCCAAAAAACCTGAGAAGTCAGCTGCTGAAGAGCCTCGTGGAGGCCTGAAGATAGAAAGTGAGACCC
  CTGTGGACATGGCTGCCAGCATGCCCTCCAGCAGGCACAAAGCAGCCACCAAGGGCTCGAGGAAACCCAA
  CATCAAAAAGGAATCAAAATCTTCTCCTCGACCTACGGCAGAGAAGAAGAAGTACAAGTCAGCCAGTAAA
  CCGTCCCAGAAGTCTCGGGAGATCATAGAGACTGACACCTCGTCCTCAGACTCGGACGGCAGCGAGAGCC
  TGCCTCCCTCCTCACAGACTCCTAAGTACCCTGAAAGCAACAGGACTCCTGTCAAACCCTCCTCAGTGGA
  GGAAGAAGATAGCTTTTTTCGGCAACGAATGTTCTCTCCTATGGAAGAGAAGGAGCTCCTTTCCCCACTC
  AGTGAGCCTGATGACAGGTATCCACTCATTGTGAAGATTGACCTCAATCTTCTGACTCGAATACCCGGGA
  AGCCTTACAAAGAAACAGAGCCACCCAAGGGGGAAAAGAAAAATGTTCCAGAGAAACACTCGAGAGAGGT
  GCAGAAGCAAGCCTCAGAAAAGGCTTCCAACAAAGGCAAGAGGAAACACAAGAATGATGATGATACCCGA
  GCCAGTGAGAGCAAAAAACCCAAAACAGAGGACAAGAACTCATCAGGCCACAAGCCATCCAGCAGCAGAG
  AGTCATCTAAGCAGAGTAGTACAAAAGAAAAGGACTTGCTACCTTCACCTGCTGGGCCCATTCTTTCAAA
  AGATTCAAAAACAGAGCATGGTTCTCGGAAGAGGACTGTCAGTCAGTCGTCGTCCTTAAAGTCCAGTGGG
  ACCAGCAGCAAAGAGAACAGTGGCAGCAGCAGCAAGAGCAGCTCCTCCTCCACAGCGAAGCAGAAGAAGA
  CTGAAGGCAAGGGTCCCAGCAGCTCCAAGGAGGCCAAGGAGAAGGCTCCAAATAGCTCCTCTAATTGTCC
  TCCGTCTACACCAACTTCTGAGTCTTCGAAGCCTCGGAGAACAAAGCTTGCCTTTGATGACAGAAATTAT
  TCAGCAGACCATTATTTACAAGAAGCAAAAAAACTCAAACACAATGCAGATGCTTTGTCTGACAGGTTTG
  AGAAAGCCGTGTACTACCTTGATGCTGTAGTGTCTTTCATTGAGTGTGGGAATGCATTAGAGAAGAACGC
  TCAGGAATCCAAGTCCCCATTCCCTATGTACTCAGACACGGTGGAGCTCATTAAGTACACTATGAAACTA
  AAGAATTATTTGGCACCAGATGCTACAGCTGCAGACAAGAGACTCACAGTACTTTGCCTGAGATGCCAGT
  CATTATTGTACCTGAGGCTGTTCAAACTGAAGAAGGAGAACGCTCTCAAGTACTCCAAGACGCTAACTGA
  GCACCTGAAGAATTCTTACAGTAATTCTCAAGCACCTTCACCCGGCTTGGGGAGCAAAGCTGTTGGGATG
  CCCTCCCCTGTTTCTCCAAAACTGTCACCAGGCAATTCTGGAAGTTACTCTTCTGGGGGAAGTAGTGCTT
  CAGCAAGTGGTTCTTCAGTGACCATTCCACAGAAAATCCACCAGATGGCAGCAAGCTATGTCCAGGTTAC
  ATCTAACTTCCTCTATGCCACAGAAATTTGGGACCAAGCTGAACAGCTTTCCAAAGAACAAAAAGAATTC
  TTTGCTGAACTGGACAAAGTGATGGGCCCTCTCATCTTTAATGCAAGCATCATGACAGATCTGGCTCGTT
  ACACCCGGCAGGGACTGCATTGGCTTCGGCAGGATGCCAAGCTGATATCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_033565
• Insert Size: 3483 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033565.2, NP_291043.1
• RefSeq Size: 10165 bp
• RefSeq ORF: 3483 bp
• Locus ID: 93736
• UniProt ID: Q9ESC8
• Cytogenetics: 11 B1.3
• Gene Summary: Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression (By similarity).[UniProtKB/Swiss-Prot Function]